Labshake search
Citations for Illumina :
9101 - 9150 of 10000+ citations for Cow Putative Phospholipase B Like 2 PLBD2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2023Quote: ... and 1.5 ug RNA was used for library preparation at the High-throughput Sequencing Facility at UNC Chapel Hill using the Stranded mRNA-Seq kit (Kapa Biosystems. The NovaSeq 6000 sequencer (Illumina) generated 50bp paired-end reads with a read depth of 23-35 million reads per biological replicate ...
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bioRxiv - Genetics 2023Quote: ... Genomic DNA libraries were prepared either with the Westburg NGS DNA Library prep kit, or with the KAPA DNA HyperPrep (Kapa Biosciences, KK8504) and adapters IDT for Illumina TruSeq DNA UD Indexes (Illumina 20022370), according to the manufacturer recommendations ...
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bioRxiv - Genomics 2023Quote: Three samples of 50 ng each of genomic DNA were sheared and processed using the Nextera DNA Library Prep Kit (Illumina, USA) according to the manufacturer’s instructions ...
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bioRxiv - Physiology 2023Quote: The cDNA library was then prepared from 500 ng of total RNA using the TruSeq stranded mRNA library kit (Illumina, USA) according to the manufactureŕs protocol ...
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bioRxiv - Plant Biology 2023Quote: ... 1 µg DNA from all the samples was used to prepare sequencing libraries with TruSeq Nano DNA HT Sample Preparation Kit (Illumina, USA) and qualified libraries were sequenced on the Illumina HiSeq2500 platform to obtain 2x150 bp reads at approximately 40x coverage ...
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bioRxiv - Cancer Biology 2023Quote: ... paired-end sequencing was performed on an Illumina NovaSeq 6000 sequencer using 100 cycle sequencing kit (Illumina Inc., San Diego, CA) to a minimum read depth of 40 M reads/library ...
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bioRxiv - Cancer Biology 2024Quote: ... Isolated nuclei were resuspended in 50 μL of Transposition mix (Illumina Tagment DNA Enzyme 100 nM, TD-Buffer 2X (kit from Illumina, 20034197), PBS 0.33% ...
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bioRxiv - Cell Biology 2024Quote: ... libraries were sequenced on a High-output flow cell (400M clusters) using the NextSeq® 500/550 High Output 150 cycles kit (Illumina), in paired- end 76/76 nt mode ...
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Dysregulated expanded endocannabinoid system as therapeutic targets of amyotrophic lateral sclerosisbioRxiv - Neuroscience 2024Quote: ... A library was independently prepared with 1 µg of total RNA for each sample by an Illumina TruSeq Stranded mRNA Sample Prep Kit (Illumina, Inc.). The first step in the workflow involved purifying the poly-A-containing mRNA molecules using poly-T-attached magnetic beads ...
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bioRxiv - Molecular Biology 2023Quote: ... 500 ng of total RNA were used for library construction with the TruSeq stranded mRNA library prep kit (Illumina, Inc., USA), including a poly-A enrichment step ...
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bioRxiv - Microbiology 2023Quote: ... dual indices and adaptors were linked to the amplicon using the Nextera XT Index Kit® (Illumina, San Diego, CA, USA) following the thermal cycler program ...
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bioRxiv - Microbiology 2023Quote: ... DNA libraries of each sample were prepared for whole genome sequencing (WGS) using a Nextera XT kit (Illumina, San Diego, CA). Briefly ...
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bioRxiv - Genomics 2023Quote: ... A total of 4 µg of RNA per sample was subjected to rRNA depletion using the RiboZero Plant Leaf kit (Illumina, MRZPL1224) following the manufacturer’s recommendations ...
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bioRxiv - Genomics 2023Quote: ... Pooled libraries were loaded at a final concentration of 750 pM and sequenced on an Illumina NextSeq 2000 using a P3 2x150 kit (Illumina Inc). De-multiplexing ...
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bioRxiv - Genomics 2023Quote: ... and pair end sequenced on the Illumina Miseq using the MiSeq Reagent Nano Kit v2 (300-cycles) (Illumina, MS-102-2002).
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bioRxiv - Neuroscience 2023Quote: ... The denatured library with a final loading concentration of 400 pM in a pool was loaded on the S4 FC using Illumina SBS kits (Illumina, 20012866) with the following setting on the NovaSeq 6000 ...
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bioRxiv - Neuroscience 2023Quote: ... and a duplicate unrelated control sample was diluted to 5 ng/µl in low TE provided in AmpliSeq Library PLUS (384 Reactions) kit (Illumina, 20019103). AmpliSeq was carried out following the manufacturer’s protocol (document 1000000036408v07) ...
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bioRxiv - Cancer Biology 2023Quote: DNA libraries were produced from 50 ng of genomic DNA using the Nextera Rapid Capture Exome kit (Illumina, San Diego, USA) as per manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: Poly-A enriched strand-specific libraries were generated with the TruSeq mRNA V2 sample preparation kit (Illumina, cat# RS-122-2001), ribosomal RNA depleted strand-specific RNA libraries with the TruSeq Stranded Total RNA LT sample preparation kit with Ribo-Zero Gold (Illumina ...
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Activation of glucocorticoid receptor signaling inhibits KSHV-induced inflammation and tumorigenesisbioRxiv - Cancer Biology 2023Quote: ... mRNA was isolated using Oligo(dT) beads and library was prepared following the Illumina TruSeq Stranded mRNA Library Prep Kit (Illumina, 20020594). Sequencing was carried out using Illumina HiSeq 3000 with the 50-bp single-read sequencing module ...
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bioRxiv - Microbiology 2023Quote: ... Sequencing libraries were prepared using the Ribo-Zero rRNA Removal Kit (Gram-positive Bacteria) (Illumina Inc., San Diego, CA, United Stated) and the TruSeqStranded mRNA library prep kit (Illumina Inc) ...
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bioRxiv - Genomics 2023Quote: ... The pool was sequenced on an entire lane of a NextSeq 500 HO flow cell to generate paired-end 151 bp reads using the NextSeq 500 High Output Kit v2.5 (300 cycles) (Illumina, Cat # 20024908).
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bioRxiv - Immunology 2023Quote: ... Illumina sequencing of the 8 samples in 100 million reads/sample was done with the NextSeq 500 High Output Kit v2 (300 cycles) sequencer (Illumina Inc) generating paired end reads of approximately 2 × 75 bp in length ...
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bioRxiv - Genomics 2023Quote: ... Ribosomal RNA was depleted from 500 ng total RNA using the Illumina Ribo-Zero Plus rRNA Depletion Kit (Illumina, Cat. # 20037135). RNA was converted to cDNA ...
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bioRxiv - Microbiology 2023Quote: ... 220 ng of total RNA was used to prepare libraries for sequencing using an Illumina TruSeq Stranded mRNA Library Prep kit (Illumina, 20020594) and SuperScript II Reverse Transcriptase (Invitrogen ...
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bioRxiv - Genomics 2023Quote: ... after RNase I digestion (50 units nuclease per 40 µg of RNA; the nuclease was included in TruSeq Mammalian Ribo Profile Kit, Illumina, RPHMR12126), adding 15 µl of SUPERase-IN (Invitrogen ...
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bioRxiv - Genomics 2023Quote: ... A total of 4 µg of RNA per sample was subjected to rRNA depletion using the RiboZero Plant Leaf kit (Illumina, MRZPL1224) following the manufacturer’s recommendations ...
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bioRxiv - Plant Biology 2023Quote: ... Samples were checked for quality with Agilent Bioanalyzer and those with > 7.0 RNA integration number were processed with the mRNA Seq Kit (Illumina, cat. # 1004814). 7 microliters of oligo dT on Sera-magnetic beads and 50 μL of binding buffer were used for isolation of mRNA ...
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bioRxiv - Physiology 2024Quote: ... and between 187 and 500 ng per sample were used as input for the TruSeq stranded mRNA library kit (Illumina, USA) following the manufacturers manual ...
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bioRxiv - Microbiology 2024Quote: ... mRNA was used to generate the cDNA library according to the NEBNext® UltraTM II RNA Library Prep Kit protocol (Illumina), which was then sequenced using the Illumina HiSeq 2000 system ...
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bioRxiv - Microbiology 2024Quote: Libraries for Illumina short read sequencing were prepared from 1 ng of extracted DNA utilizing the Nextera XT DNA Library Prep Kit according to the manufacturer’s recommendations (Illumina Inc., USA). Sequencing was carried out in paired-end (2×300 base pairs ...
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bioRxiv - Genomics 2023Quote: The 200 µL of the lysates described above were treated with RNase I (50 units nuclease per 40 µg of RNA; the nuclease was included in TruSeq Mammalian Ribo Profile Kit, Illumina, RPHMR12126) for 1 hour at room temperature with gentle mixing ...
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bioRxiv - Molecular Biology 2023Quote: ... 1 μg of total RNA was subjected to poly(A) enrichment and library preparation using the TruSeq® Stranded mRNA Library Prep Kit (Illumina) following the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2024Quote: ... The subsequent steps of library preparation were all carried out on the Agilent NGS Bravo workstation in 96-well plates following the instructions for the Illumina TruSeq Stranded mRNA kit (Illumina Cat no: 20020595, Illumina, USA). mRNA was purified from 300-800 ng of total RNA through selective-binding on poly dT-coated beads ...
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bioRxiv - Physiology 2024Quote: ... Stranded libraries were prepared with 100 ng of total RNA with the TruSeq Stranded mRNA Library Prep for NeoPrep Kit (Illumina, Inc.) per the manufacturer’s instructions ...
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bioRxiv - Genomics 2024Quote: ... Each strain had four short-read libraries prepared using the TruSeq Stranded mRNA Library Prep Kit (Illumina, San Diego, CA, USA), following the manufacturer’s recommendations ...
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bioRxiv - Developmental Biology 2024Quote: ... RNA-seq libraries were then prepared using the Illumina TruSeq RNA Library Prep Kit v2 (Illumina, catalog no. RS-122-2001), adhering to the manufacturer’s instructions ...
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bioRxiv - Genetics 2024Quote: Total RNA from mouse glomeruli was sequenced on a HiSeq 4000 following standard workflow using NEBNext Ultra II RNA Library Preparation kit (New Englab Biolabs, MA)(Illumina, CA) and paired-end sequencing (Genewiz ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Short-inserts of 150-bp paired-end libraries were prepared for each individual using a Nextera XT DNA Library Preparation Kit (Illumina, Japan) and 16 PCR cycles ...
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bioRxiv - Genetics 2024Quote: ... DNA was submitted to the Mammalian Genotyping Core at University of North Carolina to be genotyped using the Infinium Global Diversity Array-8 v.10 Kit (Illumina #20031669).
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bioRxiv - Genomics 2024Quote: ... ChIP-seq libraries were prepared from 10 ng of immunoprecipitated DNA using TruSeq ChIP Library Preparation Kit (Illumina, IP-202-1012) according to the manufacturer’s instructions with minor modifications ...
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bioRxiv - Evolutionary Biology 2024Quote: ... nerve chain and ovaries of each female was extracted using standard protocols (DNA used for ONT sequencing: Macherey-Nagel NucleoBond HMW DNA kit, DNA used for Illumina sequencing ...
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bioRxiv - Molecular Biology 2024Quote: Amplicons were sequenced on a NextSeq 500 using a NextSeq 500/550 High Output v2.5 75 cycle kit (Illumina cat #20024906) or on a NextSeq 2000 using a NextSeq 1000/2000 P2 Reagents 100 cycle kit (Illumina cat #20046811) ...
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bioRxiv - Genomics 2024Quote: ... RNA libraries for RNA-Seq were prepared using the Illumina TruSeq Stranded Total RNA Ribo-Zero Plant kit (Illumina, CA, US), and sequenced on the Illumina platform in paired-end mode and 150 bp read-length (Psomagen ...
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bioRxiv - Cancer Biology 2024Quote: ... RNA concentration was quantified using a Nanodrop and 1 µg of RNA per sample was submitted to the Abigail Wexner Research Institute Genomic Services Laboratory for library preparation using the TruSeq Stranded mRNA Kit (Illumina 20020594), library quality control ...
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bioRxiv - Genomics 2024Quote: 1 ng of psoralen pull-down DNA (or corresponding Input DNA from the same cells without pull-down) was used to prepare sequencing libraries using a Nextera XT library prep kit (Illumina, Inc) according to the manufacturer protocol ...
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bioRxiv - Cancer Biology 2024Quote: ... Libraries were prepared by the University of Colorado Genomics Shared Resource using Illumina TruSeq Stranded mRNA Library Prep kit (Illumina, #20020595) and sequenced using paired end 50 cycles 2 x 50 on the NovaSEQ 6000 with >30 million reads per sample obtained ...
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bioRxiv - Plant Biology 2024Quote: RNA-seq libraries were constructed by the POPS platform (IPS2) using the Illumina Stranded mRNA Prep kit (Illumina®, California, U.S.A.) according to the supplier’s instructions ...
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bioRxiv - Developmental Biology 2024Quote: ... The Tagment DNA Buffer and TDE1 enzyme are both contained in the Tagment DNA TDE1 Enzyme and Buffer Kit (Illumina, 20034197). The transposed chromatin was isolated using the Minelute PCR Cleanup kit (Qiagen ...
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bioRxiv - Molecular Biology 2024Quote: ... The nuclei were tested for viability using DAPI and 50,000 nuclei were taken for tagmentation reaction as per the manual (Illumina tagmentation kit). The processed samples were subjected to library preparation similar to ChIP-seq methods.