Labshake search
Citations for Illumina :
8651 - 8700 of 9681 citations for ssc mir 758 Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... RNA concentration was quantified using a Nanodrop and 1 µg of RNA per sample was submitted to the Abigail Wexner Research Institute Genomic Services Laboratory for library preparation using the TruSeq Stranded mRNA Kit (Illumina 20020594), library quality control ...
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bioRxiv - Genomics 2024Quote: 1 ng of psoralen pull-down DNA (or corresponding Input DNA from the same cells without pull-down) was used to prepare sequencing libraries using a Nextera XT library prep kit (Illumina, Inc) according to the manufacturer protocol ...
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bioRxiv - Cancer Biology 2024Quote: ... Libraries were prepared by the University of Colorado Genomics Shared Resource using Illumina TruSeq Stranded mRNA Library Prep kit (Illumina, #20020595) and sequenced using paired end 50 cycles 2 x 50 on the NovaSEQ 6000 with >30 million reads per sample obtained ...
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bioRxiv - Plant Biology 2024Quote: RNA-seq libraries were constructed by the POPS platform (IPS2) using the Illumina Stranded mRNA Prep kit (Illumina®, California, U.S.A.) according to the supplier’s instructions ...
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bioRxiv - Developmental Biology 2024Quote: ... The Tagment DNA Buffer and TDE1 enzyme are both contained in the Tagment DNA TDE1 Enzyme and Buffer Kit (Illumina, 20034197). The transposed chromatin was isolated using the Minelute PCR Cleanup kit (Qiagen ...
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bioRxiv - Molecular Biology 2024Quote: ... The nuclei were tested for viability using DAPI and 50,000 nuclei were taken for tagmentation reaction as per the manual (Illumina tagmentation kit). The processed samples were subjected to library preparation similar to ChIP-seq methods.
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bioRxiv - Developmental Biology 2024Quote: ... and samples with an RNA Integrity Number (RIN) >8 were used to generate RNA sequencing libraries using the Illumina® Stranded mRNA Prep kit (Illumina).
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bioRxiv - Plant Biology 2024Quote: ... Index-coded samples were clustered on a cBot Cluster Generation System using TruSeq PE Cluster Kit v3-cBot-HS (Illumina, USA) and sequenced on an Illumina Novaseq platform to generate 150bp paired-end reads with a sequencing depth of 10 million reads per sample ...
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bioRxiv - Microbiology 2024Quote: ... Purified 16S rRNA gene sequences were analyzed using a MiSeq Reagent Kit v3 and MiSeq system (Illumina, San Diego, CA, USA). Raw 16S rRNA gene sequences were analyzed using QIIME 2 (ver.2021.4 ...
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bioRxiv - Molecular Biology 2024Quote: ... validated for activity in a corresponding assay and three clones of each cell line were subjected to a genotyping array screening using a Infinium Global Screening Array-24 Kit (Illumina, #20030770) to check for chromosomal rearrangements in iPSCs clones ...
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bioRxiv - Microbiology 2024Quote: ... Metatranscriptomic libraries were prepared using the Illumina Stranded Total RNA library preparation kit with ribosomal depletion using Ribo-Zero Plus (Illumina Inc.). Short read Illumina NovaSeq 6000 sequencing on an SP flow cell of 2X150 bp was performed at the SNP/SEQ facility of the National Genomics Infrastructure in Uppsala ...
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bioRxiv - Synthetic Biology 2024Quote: ... Barcoded RNA-seq libraries were prepared from 1 μg of total RNA using the TruSeq Stranded mRNA Library Prep kit according to the manufacturer’s instructions (Illumina Inc., USA). RNA-seq libraries were sequenced on an Illumina NovaSeq 6000 System according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2024Quote: ... and strand-specific transcriptome sequencing (RNA-Seq) libraries were constructed using the KAPA RNA HyperPrep kit (Illumina, San Diego, CA, USA). Sequencing was carried out on an Illumina NextSeq 500 instrument at the BPF Next-Gen Sequencing Core Facility at Harvard Medical School ...
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bioRxiv - Microbiology 2024Quote: ... Libraries for all samples were prepared using the Nextera™ DNA Flex Library Prep Kit (Illumina Inc., San Diego, CA, USA), and the subsequent shotgun sequencing was executed on a NovaSeq 6000 system (Illumina Inc. ...
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bioRxiv - Microbiology 2024Quote: ... The genomic libraries were then sequenced on a MiSeq instrument with a MiSeq Reagent Kit v2 (300 cycles; 2 x 150 bp) (Illumina Inc.).
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bioRxiv - Microbiology 2024Quote: ... using 2 x 100-bp paired-end reads and 2 x 12-bp index reads with a 300-cycle kit (Illumina, 20012860).
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bioRxiv - Neuroscience 2024Quote: ... Eluted libraries were quantified using a Qubit dsDNA HS Assay Kit and sequenced on a NovaSeq 6000 (Illumina, San Diego, California).
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bioRxiv - Microbiology 2024Quote: ... Amplicons were sequenced using Illumina’s v3 600-cycle (paired-end) reagent kit on a MiSeq (Illumina Inc., San Diego, CA, USA). Reads were demultiplexed based on the combination of index (CASAVA 1.8 ...
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bioRxiv - Microbiology 2024Quote: ... The cDNA libraries were constructed using the NEBNext® Ultra II Directional RNA Library Prep Kit for Illumina® and sequenced in paired-end (2×150 bp) on a NovaSeq 6000 (Illumina). After the confirmation of multiple virus infections in H ...
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bioRxiv - Microbiology 2024Quote: ... Sequence libraries were prepared and pooled using the DNA prep and the NextSeq 500 mid-output reagent kits (Illumina, CA, USA). Illumina NextSeq 500 platform ...
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bioRxiv - Neuroscience 2024Quote: ... Half of the obtained dephosphorylated footprint RNA was processed further for rRNA depletion using Ribo-Zero Gold rRNA Removal Kit (Human/Mouse/Rat) (Illumina, MRZG12324). For each reaction ...
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bioRxiv - Cancer Biology 2021Quote: ... Either 250ng or 75ng of gDNA were used for library preparation with either a Truseq Custom Amplicon Kit (Illumina, San Diego, USA) or an Ampliseq for Illumina Custom Panel (Illumina ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were lysed for 5 min followed by transposase reaction and library amplification using Nextera DNA Library Prep Kit (Illumina, California, USA). Libraries were then size-selected (240-360 bp ...
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bioRxiv - Molecular Biology 2021Quote: ... 1 ug of treated RNA was then input into the Illumina TruSeq Small RNA Library Preparation Kit (Illumina; Cat #: RS-200-0012) with 11 cycles of PCR amplification ...
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bioRxiv - Microbiology 2021Quote: ... The library was generated using the transposon-based Nextera™ XT Sample Preparation Kit using 15 cycles (Illumina, San Diego, CA, USA) and the concentration of DNA libraries was measured by Quant-iT™ DNA HS Assay Kit ...
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bioRxiv - Evolutionary Biology 2020Quote: ... The barcoded stranded mRNA-sequencing libraries were prepared using the Illumina TruSeq mRNA v2 sample preparation kit (Illumina, San Diego, CA, USA) with poly-A mRNA enrichment ...
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bioRxiv - Developmental Biology 2020Quote: Libraries were sequenced on an Illumina HiSeq X™ using HiSeq X™ Five Reagent Kit v2(Illumina, Cat# FC-502-2021).
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bioRxiv - Evolutionary Biology 2021Quote: ... We then prepared tagmentation-based whole genome libraries for low coverage sequencing by enzymatically shearing DNA diluted to approximately 2.5 ng/ul using the Illumina Tagment DNA TDE1 Enzyme and Buffer Kits (Illumina, San Diego, CA) at 55°C for 5 minutes ...
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Host transcriptomic profiling of COVID-19 patients with mild, moderate, and severe clinical outcomesbioRxiv - Genomics 2020Quote: ... as previously described.12,13,14 Libraries were then sequenced using the NovaSeq SP Reagent kit (2 × 150 cycles) from Illumina (San Diego, CA, USA) to generate a minimum of 15M reads per sample (Supplementary Table 1).
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bioRxiv - Genomics 2020Quote: ... Complementary RNA (cRNA) was synthesized and biotinylated using the Illumina™ TotalPrep™-96 RNA Amplification Kit (Illumina Inc., San Diego, CA). cRNA samples were then hybridized to HumanHT-12 v3 Expression BeadChips (Illumina ...
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bioRxiv - Synthetic Biology 2022Quote: ... Indexes were added to the amplified DNA using i5 and i7 primers from the Nextera XT Index Kit (Illumina # FC-131-1002). Indexed samples were loaded into a MiSeq Reagent Kit v3 600-cycle (Illumina # MS-102-3003 ...
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bioRxiv - Bioengineering 2022Quote: ... Illumina sequencing of the spirulina genome was performed on a MiSeq instrument using a MiSeq Reagent Kit v2 (Illumina, MS-102-2003).
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bioRxiv - Genomics 2022Quote: ... USA) from which ~500bp insert indexed sequencing libraries were generated using the Illumina TruSeq v2 Library Construction Kit (Illumina, San Diego, CA). Single-end 100-bp reads were generated from D ...
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bioRxiv - Neuroscience 2021Quote: ... Hippocampus RNA-seq libraries were prepared in accordance with New England Biolab protocols (NEBNext_ Ultra II RNA library Prep kit for Illumina, NEB, USA). Briefly ...
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bioRxiv - Molecular Biology 2021Quote: ... Libraries were prepared using an Illumina® TruSeq® Stranded Total RNA kit with ribosomal depletion by Ribo-Zero Gold solution from Illumina® according to the manufacturer’s protocol and sequenced on an Illumina® HiSeq 4000 (2x 75 bp).
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bioRxiv - Molecular Biology 2021Quote: ... Individual RNA-Seq libraries were prepared from each pool of extracted RNA using the ScriptSeq™ v2 RNA-Seq library preparation kit (Illumina, USA), using 12 cycles of PCR amplification ...
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bioRxiv - Genomics 2020Quote: ... The library was constructed using the Illumina TruSeq DNA Sample preparation kit according to manufacturer’s instructions (Illumina, Inc., San Diego, CA, USA). Next-generation sequencing data was obtained using the Illumina HiSeq 2000 ...
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bioRxiv - Genomics 2020Quote: ... One hundred ng of total RNA was used to construct an Illumina sequencing library using the TruSeq RNA-seq Sample Prep kit according to the manufacturer’s recommended protocols (Illumina, San Diego, USA). The libraries were sequenced for 101 or 151 bp paired-ends on an Illumina HiSeq2500 sequencer using the standard protocol (Illumina).
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bioRxiv - Immunology 2021Quote: ... The pool was titrated to 1.8 -1.9 pM and sequenced as 2 × 75 bp reads in a total of nine runs on the NextSeq 550 instrument using the high output v2.5 kit (Illumina, San Diego, CA).
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bioRxiv - Genomics 2020Quote: ... Genomic DNA libraries containing 300∼500 bp fragments were constructed using Nextera XT DNA Library Preparation Kit (Illumina Inc., San Diego, USA). The WGS was performed using 100 bp paired-end sequencing protocol under Illumina platform using HiSeq4000 sequencer (Macrogen ...
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bioRxiv - Microbiology 2019Quote: ... The sequencing library was prepared by fragmenting and tagging the purified gDNA using the Nextera XT DNA Sample Preparation Kits (Illumina, Cambridge, UK) to manufactures instructions ...
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bioRxiv - Genomics 2019Quote: TruSeq libraries were prepared with 1 µg of total RNA using the TruSeq Stranded mRNA Library Prep Kit (Illumina, #RS-122-2101) and following the manufacturer's instructions ...
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bioRxiv - Bioengineering 2020Quote: ... cDNA libraries were single-end sequenced in 76 cycles using a NextSeq 500 Kit v2 (FC-404-2005, Illumina, San-Diego, CA). High-throughput sequencing was performed using NextSeq500 sequencing system (Illumina ...
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bioRxiv - Systems Biology 2019Quote: ... denatured in NaOH and combined with HT1 plus a 1% PhiX spike at a final running concentration of 10pM.The flow-cell was clustered using HiSeq PE Cluster Kit v3 (Illumina, PE-401-3001) utilising the Illumina PE HiSeq Cluster Kit V3 cBot recipe V8.0 method on the Illumina cBot ...
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bioRxiv - Genomics 2019Quote: ... The cBot Cluster Generation System was used to cluster index-coded samples in libraries by TruSeq PE Cluster Kit v3-cBot-HS (Illumina, CA, USA). In each library ...
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bioRxiv - Microbiology 2019Quote: ... Normalized library pools were sequenced on an Illumina MiSeq machine using the 600-cycle MiSeq Reagent Kit v3 (Illumina, MS-102-3003). All FASTQ files are available on request.
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Integrative analysis of hexaploid wheat roots identifies signature components during iron starvationbioRxiv - Plant Biology 2019Quote: ... libraries were prepared from the total RNA using Illumina TruSeq stranded mRNA library prep kit as per the instructions (Illumina Inc., USA). The generated mean of the libraries fragment size distribution was 559 bp ...
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bioRxiv - Plant Biology 2019Quote: Poly(A)-enriched RNA-sequencing (mRNA-seq) libraries were prepared using the Illumina TruSeq Stranded mRNA Sample Preparation Kit (Illumina, CA, USA) using 1.3 μg input of extracted total RNA ...
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bioRxiv - Microbiology 2019Quote: ... Paired-end sequencing (2 × 300 bp) was conducted on Illumina MiSeq using MiSeq v3 600-cycle kit (Illumina, San Diego, CA, USA). Sequence data was processed through MiSeq Reporter - BaseSpace for FASTQ Workflow generation followed by taxonomic classification based on Greengenes database (http://greengenes.lbl.gov/) ...
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bioRxiv - Genomics 2019Quote: ... The normalised TruSeq 550 bp and Nextera XT libraries were loaded on Illumina NextSeq 500 platform using NextSeq 500/550 v2 sequencing reagent kit (Illumina Inc., USA) and 150 bp paired-end sequencing was performed ...