Labshake search
Citations for Illumina :
8401 - 8450 of 9193 citations for Mouse CXCL2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2020Quote: ... A second round of PCR was used to add the dual indices with the Nextra XT Index Kit (Illumina, San Diego, CA, USA). The quality of these sequencing libraries was determined by the Qubit® 2.0 Fluorometer ...
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bioRxiv - Developmental Biology 2021Quote: ... One microgram of total RNA was used to create each sequencing library using a Truseq RNA Sample Preparation Kit (Illumina, RS-122-2001). Briefly ...
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bioRxiv - Developmental Biology 2021Quote: ... ATAC-seq libraries for murine endothelial cells were processed as previously described (Buenrostro et al., 2015) and libraries were generated using the Nextera DNA Sample Preparation Kit (Illumina, FC-121-1030). The quality of purified DNA libraries was checked by Agilent High Sensitivity DNA kit (Agilent Technologies) ...
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bioRxiv - Developmental Biology 2021Quote: ... 500 ng of total RNA from each sample was used for the RNA-seq library preparation using the TruSeq stranded mRNA kit (Illumina, San Diego, CA) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... were prepared from HepG2 cell samples using 200–500 ng of total RNA following the manufacturer’s instructions for the TruSeq RNA kit (Illumina, San Diego, CA, USA). With this kit ...
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bioRxiv - Cell Biology 2019Quote: ... The rRNA depleted samples were fragmented and sequencing libraries prepared using the TruSeq Stranded Total RNA Library Prep Kit (Illumina, San Diego, CA). Briefly ...
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bioRxiv - Microbiology 2021Quote: ... Fragments were then indexed with 10 nucleotide barcode sequences using Nextera XT Index Kit v2 Set D primers (Illumina, San Diego, California, USA). Indexing PCR reactions were run in triplicate with a volume of 25 μL using 12.5 μL NEB Next High-Fidelity Master Mix ...
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bioRxiv - Developmental Biology 2021Quote: ... Libraries were prepared starting from 1000 ng of total RNA using the RNA Sample Prep Kit v2 (Illumina Inc, San Diego, CA, USA) including a poly-A selection step following the manufacturer’s instructions and sequenced as 2 x 100 nt paired-end reads using an Illumina HiSeq 2500 ...
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bioRxiv - Microbiology 2021Quote: ... Jude Children’s Research Hospital Hartwell Center with DNA libraries prepared using Nextera XT DNA-Seq library prep kits (Illumina, cat#FC-131-1024) with 96 dual-index bar codes and sequenced on an Illumina MiSeq personal genome sequencer ...
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bioRxiv - Genomics 2019Quote: ... We prepared cDNA libraries for the three biological replicates per genotype using TruSeq Stranded mRNA LT Sample Prep Kit (Illumina, San Diego, CA), and performed single-end sequencing using Illumina HiSeq 2000 at the Penn State Genomics Core Facility to obtain 100 bp reads at an average coverage of 36.0 million aligned reads/sample ...
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bioRxiv - Developmental Biology 2021Quote: ... RNA-seq libraries were generated using Illumina SureCell WTA 3’ Library Prep Kit for the ddSEQ System (6 cartridge version, cat.no. 20014280, Illumina, San Diego, CA, USA). Libraries were assessed for quality ...
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bioRxiv - Evolutionary Biology 2020Quote: ... was isolated by magnetic bead and converted into RNA libraries using the Illumina TruSeq RNA Library preparation kit v2 according to the manufacturer’s instructions (Illumina, San Diego, CA, USA). Libraries were sequenced on a HiSeq 2100 ...
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bioRxiv - Microbiology 2021Quote: ... The library was loaded at 6 pM and sequenced using the Miseq Reagent Kit v3 (600-cycle) (Illumina; catalog no., MS-102-3003).
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bioRxiv - Evolutionary Biology 2021Quote: ... Paired-end libraries were constructed from 100 ng of the total RNAs using TruSeq RNA Library Prep kits v2 (Illumina K.K., Tokyo, Japan) following the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2021Quote: ... A paired-end library was constructed from 1 µg of the DNA using TruSeq DNA PCR-Free LT Sample Prep kits (Illumina K.K., Tokyo, Japan) following the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2020Quote: ... A total amount of 3μg RNA per sample was used for mRNA purification and library construction via the Truseq™ RNA Sample Prep Kit (Illumina, CA, USA) followed the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2020Quote: ... libraries were prepared from 1 ng of cDNA using the Nextera® XT DNA Library Preparation Kit (Illumina Inc., San Diego, CA USA) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2020Quote: ... both ribosome footprinting and total RNA samples were depleted of rRNA using the Ribo-Zero Gold rRNA Removal Kit (H/M/R) (Illumina, catalog no. MRZG126) with the modification that the 50°C incubation was not performed for the ribosome footprinting samples ...
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bioRxiv - Developmental Biology 2020Quote: ... prior to pooling and sequencing using the Illumina NextSeq 500 platform using a custom primer and the High Output v2 kit (75 cycles) (Illumina, #FC-404-2005). The library loading concentration was 2.2 pM and sequencing configuration as following ...
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bioRxiv - Developmental Biology 2020Quote: ... 500 ng of total RNA was used for each RNA-seq library preparation using a TruSeq Standard mRNA kit (Illumina, San Diego, CA) as described in our previous publications (Khristi et al. ...
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bioRxiv - Developmental Biology 2021Quote: ... This nuclear pellet was used to prepare ATAC-seq libraries using the Nextera DNA Library Preparation Kit (Illumina, San Diego, CA, Cat #FC1211030). The pellet was suspended in 5 μL buffer TD before adding 2.5 μL water and 2.5 μL tagment DNA enzyme ...
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bioRxiv - Developmental Biology 2021Quote: Approximately 50,000 nephron progenitor cells were used to generate each sequencing library for the assay for transposase-accessible chromatin (ATAC-seq) using the Nextera DNA Flex Library Prep Kit (Illumina FC-121-1030) with modifications according to a previously published method (Buenrostro et al. ...
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Host transcriptomic profiling of COVID-19 patients with mild, moderate, and severe clinical outcomesbioRxiv - Genomics 2020Quote: ... RNA libraries were then prepared for shotgun transcriptome sequencing using the TruSeq Stranded Total RNA Library kit from Illumina (San Diego, CA, USA) as previously described.12,13,14 Libraries were then sequenced using the NovaSeq SP Reagent kit (2 × 150 cycles ...
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bioRxiv - Genomics 2020Quote: ... The remaining 74 paired-end short insert (350 nt) sequencing libraries were constructed using 100 ng sample DNA and the TruSeq DNA Nano kit (Illumina, San Diego, CA) with coverage between 8-40x (Kitchen et al ...
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bioRxiv - Genomics 2020Quote: ... Multiple adapters were used for pooling libraries (KAPA Single-Indexed Adapter Kit KK8700 and NEBNext® Multiplex Oligos for Illumina NEB #E7535S/L). The DNA samples were sequenced using the Illumina NextSeq 500 platform and 150-nucleotide single-end reads were generated ...
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bioRxiv - Genomics 2022Quote: ... One ng of the pooled amplicons was used for library construction with the Nextera XT DNA library preparation kit (#FC-131–1096; Illumina, San Diego, CA), according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... Beads were resuspended in 25 μl of 2X TD Buffer and combined with Tn5 enzyme from the Nextera DNA Sample Preparation Kit (Illumina, FC-121-1030) and water to final volume 50 μl ...
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bioRxiv - Molecular Biology 2021Quote: ... and 2.5 µl of each Nextera Index 1 (N7XX) and Nextera Index 2 (N5XX) primers from a Nextera DNA Sample Preparation Index Kit (Illumina, FC-121-1011). PCR was performed according to the following protocol ...
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bioRxiv - Molecular Biology 2021Quote: ... The beads were resuspended in 50 μl of the following PCR master mix with indexed primers from the Nextera DNA Sample Preparation Index Kit (Illumina, FC-121-1011): Phusion HF 2X (NEB ...
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bioRxiv - Molecular Biology 2021Quote: ... Granulosa cells were lysed and tagmentation was performed on the nuclei using the Illumina Tagment DNA TDE1 Enzyme and Buffer Kits (Illumina, San Diego, USA). DNA purification was performed using the Monarch PCR & DNA Cleanup Kit (NEB ...
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bioRxiv - Molecular Biology 2020Quote: ... The resulting library was diluted to 10-pM in 600-μL of HT1 hybridization buffer (Illumina Nextera XT kit Cat#FC-131-1024) and 10-μL was loaded onto a 300-cycle MiSeq Nano v2 flow cell (Illumina Cat#MS-102-2002 ...
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bioRxiv - Molecular Biology 2020Quote: ... Oxford Nanopore Technologies’ Direct RNA sequencing (DRS) libraries were constructed as follows: intracellular RNA was immediately ribo-depleted using Ribo-Zero kits (Illumina, San Diego, CA) upon RNA extraction and used within 24 hours of ribodepletion (stored on ice) ...
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bioRxiv - Molecular Biology 2021Quote: ... DNA quality control was performed in a fragment analyzer and sequencing libraries were constructed using a Nextera DNA Flex kit (now renamed to Illumina DNA Prep, Illumina, San Diego, CA) and 2×150 BP paired-end sequencing was performed in an Illumina NextSeq500 ...
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bioRxiv - Molecular Biology 2019Quote: ... Genomic DNA from approximately 15-20 progeny per mutant were pooled and sequencing libraries were prepared with a Nextera kit (Illumina, FC-121-1030). All whole genome sequencing data is available on NCBI SRA (accession #PRJNA526508) ...
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bioRxiv - Evolutionary Biology 2022Quote: Genomic DNA was extracted from Bergerac and N2 control lines as previously described (Konrad et al. 2018) with libraries prepared using the Nextera DNAflex library kit (Illumina, San Diego, CA). Libraries were sequenced on the Illumina Novaseq6000 platform (2×150 bp ...
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bioRxiv - Genomics 2019Quote: For the bacterial DNA libraries for sequencing on Illumina platform were prepared applying Nextera XT DNA Library Preparation Kit (Illumina, San Diego, USA) with small modifications (Baym et al. ...
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bioRxiv - Genomics 2020Quote: ... The dissolved DNA was then tagmented using 0.6 μl TD Tagmentation buffer and 0.3 μl ATM Tagmentation enzyme from Nextera DNA Library Prep Kit (Illumina, catalog no. FC-121-1030) for 5min at 55 °C ...
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bioRxiv - Genomics 2020Quote: ... The 300 ng gDNA of all four samples were used to prepare paired-end libraries with the Nextera ™ DNA Flex Library Preparation kit with an average insert size of 600 bp for all four samples according to the manufacturer’s instructions (Illumina Inc., San Diego, CA).
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bioRxiv - Genomics 2020Quote: ... Approximately 1 μg of amplified DNA was sequenced by our core sequencing facility with a 600-cycle MiSeq Reagent Kit v3 (Illumina, MS-102-3003) following the manufacturer’s instructions ...
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bioRxiv - Genomics 2020Quote: ... and libraries were constructed by the Lexogen QuantSeq 3’ mRNA-Seq Library Kit FWD (Lexogen, Vienna, Austria).19 All RNA libraries were sequenced by the Illumina HiSeq4000 (Illumina, San Diego, CA). Raw sequencing data were aligned to the reference genome (GRCh37 ...
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bioRxiv - Genomics 2020Quote: ... we first generated pseudo-microarray variant calls from GIAB benchmark variants of HG002 and HG005 in the benchmark regions by extracting the variant sites used by a popular commercial microarray kit (Illumina Infnium OmniExpress-24). We obtained the microarray sites from the CSV manifest file (GRCh38 version ...
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bioRxiv - Molecular Biology 2021Quote: ... The library was prepared with PCR products pooled in equimolar amounts following the manufacture’s protocol and loaded in a Micro MiSeq Reagent Kit v2 (500-cycles) (Illumina, San Diego, CA, USA) on a MiSeq platform (Illumina) ...
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bioRxiv - Microbiology 2019Quote: ... 2 µL of each P7 and P5 of Nextera XT Index Kit v2 index primers (Illumina Catalogue No. FC-131-2001 to 2004) were added to each well ...
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bioRxiv - Genomics 2019Quote: ... Four libraries were paired-end sequenced (75 nt each) with the NextSeq 500 using the Mid Output v2 kit (150 cycles) (Illumina, #FC-404-2001).
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bioRxiv - Genomics 2019Quote: ... prior to pooling and sequencing using the Illumina NextSeq 500 platform using custom ReadOne primer (IDT) and the High Output v2 kit (75 cycles) (Illumina, #FC-404-2005). The library loading concentration was 2.2 nM ...
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bioRxiv - Plant Biology 2019Quote: ... Libraries for RNA expression analysis were prepared in duplicates from 2 μg of total RNA using the TrueSeq Stranded mRNA Sample Prep Kit (Illumina, San Diego, CA) following the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2019Quote: 1 µg of total RNA from each sample was used for RNA sequencing library preparation with the TruSeq® Stranded mRNA Library Prep kit (Illumina, catalog # 20020594). The libraries were barcoded by TruSeq® RNA Unique Dual Indexes (Illumina ...
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bioRxiv - Developmental Biology 2019Quote: ... and ∼1 ug of each sample was provided to the Genome Sequencing Core at the University of Kansas for library preparation using the TruSeq stranded mRNA kit (Illumina, San Diego, CA). Single Read 100 (SR100 ...
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bioRxiv - Cancer Biology 2019Quote: ... mRNA sequencing libraries were generated from 50 ng total RNA using the Illumina Neoprep TruSeq stranded mRNA library prep kit (Illumina, NP-202-1001). RNA libraries were sequenced paired-end (2 × 75 bp ...
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bioRxiv - Cancer Biology 2020Quote: ... Libraries were pooled and sequenced (paired-end 2*75bp) on a NextSeq500 using the NextSeq 500/550 High Output Kit v2 according to the manufacturer’s instructions (Illumina, San Diego, CA, USA). Raw sequencing data generated by the Illumina NextSeq500 instrument were mapped to the mouse reference genome using the hisat2 software(Kim et al. ...