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Citations for Illumina :
751 - 800 of 1014 citations for Tick Borne Encephalitis Virus Envelope Protein Human Fc tag since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... Single-indexed and multiplexed samples were run on an Illumina Next Seq 500 sequencer using a NextSeq 500 v2 kit (FC-404-2005; Illumina, Can Diago, CA) for paired-end sequencing.
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bioRxiv - Immunology 2021Quote: ... Libraries were quantified sing Qubit High-Sensitivity DNA kit and preparation kit and libraries were constructed using Nextera XT DNA tagmentation (Illumina FC-131-1096) using 800 pg of pooled cDNA library as input using index primers with format as in Gierahn et al [46] ...
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bioRxiv - Immunology 2022Quote: ... and pooled to form an equimolar sequencing library that was denatured and diluted to 1.2 pM with a 20% PhiX v3 control library (Illumina, Cat#FC-110-3001), as described in the Illumina Denature and Dilution protocol (Illumina ...
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bioRxiv - Microbiology 2022Quote: Sequencing libraries were prepared from 1 ng of each VSG PCR product using the Nextera XT DNA Library Preparation Kit (Illumina, FC-131-1096) following the manufacturer’s protocol except for the final cleanup step ...
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bioRxiv - Genomics 2022Quote: ... Indexed libraries were generated using Illumina Nextera XT DNA Library preparation Index Kit (Illumina, Cat No. FC-131-1096 and CF-131-1002), which is capable of multiplexing (up to 96 available indexes) ...
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bioRxiv - Genomics 2022Quote: ... Indexed libraries were generated using Illumina Nextera XT DNA Library preparation Index Kit (Illumina, Cat No. FC- 131-1096 and CF-131-1002). We performed 125 nt paired end sequencing for each library independently on the same chip (one library per lane ...
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bioRxiv - Genomics 2022Quote: ... 2 µL of each P7 and P5 of Nextera XT Index Kit v2 index primers (Illumina Catalogue No. FC-131-2001 to 2004) were added to each well ...
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bioRxiv - Microbiology 2022Quote: ... Purified libraries were sequenced on Illumina MiSeq platform (reagent kits: v2 300-cycles, paired-end mode) at 8 pM loading concentration with 25% PhiX spike-in (Illumina FC-110-3001). Custom sequencing primers were spiked into reagent cartridge (well 12 ...
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bioRxiv - Neuroscience 2023Quote: ... Multiplex samples Pool (1.65 pM including PhiX 1%) was loaded in NextSeq 500/550 High Output v2 kit (75 cycles) cartridge (catalog #FC-404-1005; Illumina, San Diego, CA) and loaded on NextSeq 500 System machine (Illumina) ...
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bioRxiv - Genomics 2022Quote: ... spun at 300G for 12 min at 4 ºC and resuspended in 20 uL transposition mix: 1X TD buffer and 2 uL of TDE1 (Illumina #FC-121-1030), 0.01% Digitonin in DMSO ...
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bioRxiv - Molecular Biology 2022Quote: ... before final pooling and subsequent run on a MiniSeq Sequencing System using the Miniseq High Output Kit (75 cycles) (Illumina #FC-420-1001) with a 10% of phiX spike-in.
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bioRxiv - Biophysics 2023Quote: ... The DNA concentration was measured using the Qubit dsDNA BR assay kit and sequenced using a HiSeq SBS v4 50 cycle kit (Illumina FC-401-4002) and HiSeq SR Cluster Kit v4 (Illumina GD-401-4001 ...
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bioRxiv - Immunology 2023Quote: ... 100,000 nuclei per sample were isolated using an iodixanol gradient and ATAC-seq was subsequently performed according to the original protocol (Buenrostro et al, 2013) using Nextera DNA Sample preparation kit (Illumina, FC-121-1030). After library amplification ...
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bioRxiv - Genomics 2023Quote: ... The filtered nuclei were pelleted at 500 r.c.f for 5 min in a pre-chilled fixed-angle centrifuge and then resuspended in 25 μl of 1.25x Tagment DNA Buffer (Nextera XT Kit, Illumina Inc. FC-131-1024). For cell cultures ...
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bioRxiv - Cancer Biology 2023Quote: ... of libraries were pooled and subjected to sequencing of approximately 20 million reads per sample using the Mid Output v2 kit (Illumina, FC-404-2001) on a Illumina NextSeq550 following the manufacturer’s instructions.
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bioRxiv - Genomics 2023Quote: ... about 1.5 ng purified pre-amplified cDNA sample was fragmented in four 20 μl tagmentation mix (10 μl 2x TD buffer, 5 μl Nextera XT (Illumina, FC-131-1096), and 5 μl cDNA sample ...
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bioRxiv - Genetics 2023Quote: ... Pooled libraries were diluted and processed for 35-bp paired-end sequencing on an Illumina NextSeq 500 instrument (ID: NB501524) using the NextSeq 500 High Output v2 kit (75 cycles) (Illumina; FC-404-2005) in accordance with the manufacturer’s instructions.
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bioRxiv - Genomics 2023Quote: ... The pelleted nuclei were gently resuspended with 50 µl of transposition reaction containing 25 µL 2X Tagmentation buffer (Illumina Cat FC-121-1030), 5 µL Tn5 transposase (Illumina Cat FC-121-1030 ...
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bioRxiv - Genetics 2023Quote: ... Pooled libraries were diluted and processed for either 75-bp single-end or 35-bp paired-end sequencing on an Illumina NextSeq 500 instrument (ID: NB501524) using the NextSeq 500 High Output v2 kit (75 cycles) (Illumina; FC-404-2005) in accordance with the manufacturer’s instructions.
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bioRxiv - Developmental Biology 2023Quote: ... 600 pg of pre-amplified cDNA were then used as input for Tn5 transposon tagmentation by the Nextera XT DNA Library Preparation Kit (Illumina, FC-131-1096) followed by 12 cycles of library amplification ...
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bioRxiv - Biochemistry 2023Quote: ... The DNA concentration was measured using the Qubit dsDNA BR assay kit and sequenced using a HiSeq SBS v4 50 cycle kit (Illumina FC-401-4002) and HiSeq SR Cluster Kit v4 (Illumina GD-401-4001 ...
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bioRxiv - Genomics 2023Quote: ... The tagmentation and library preparation was performed with the Nextera DNA Library Preparation Kit with 8 cycles of PCR amplification (Illumina #FC-121-1030).
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bioRxiv - Genomics 2023Quote: ... 4 μl of 1 ng amplicon DNA was combined with a mix containing 1 μl of Amplicon Tagment Mix (Illumina, FC- 131-1096) and 5 μl of Tagment DNA Buffer (Illumina ...
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bioRxiv - Developmental Biology 2023Quote: ... Libraries were generated from the resulting cDNA (0.2ng/ul per sample) using the Nextera XT DNA library preparation kit (Illumina, Cat.no. FC-131-1024) as previously described68 ...
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bioRxiv - Cancer Biology 2023Quote: ... The cell pellet was resuspended in Illumina TD buffer and 2.5 µl of Tn5 enzyme was added to the transposition reaction (Illumina, Cat # FC-121-1030). Nuclei were incubated at 37°C for 60 minutes and fragmented DNA was purified using the MinElute kit (Qiagen ...
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bioRxiv - Systems Biology 2023Quote: ChIP-seq and input libraries were sequenced on the Illumina NextSeq 500 system with a ∼20% phiX spike-in (Illumina, FC-110-3001) to generate 75 bp single-end reads (NextSeq 500/550 High Output v2 kit) ...
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bioRxiv - Cell Biology 2023Quote: ... prior to pooling and sequencing using the Illumina NextSeq 500 platform using a custom primer and the High Output v2 kit (75Lcycles) (Illumina, #FC-404-2005). The library loading concentration was 2.4 pM ...
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bioRxiv - Genomics 2023Quote: ... a total of 0.75 ng of DNA was combined with 2.5 µl 1/35th diluted transposome (Illumina; purchased in kit # FC-121-1011), and 1X Tris Buffer (10X Tris Buffer ...
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bioRxiv - Physiology 2023Quote: ... Each donor library pool was sequenced twice using the Illumina NextSeq500 with NextSeq 500/550 High Output v2 or v2.5 75-cycle kits (Illumina, USA; FC-404-2005). Libraries were sequenced using the following cycle parameters ...
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bioRxiv - Genomics 2023Quote: ... Isolated nuclei were lysed and transposed for 30 minutes at 37°C using the prokaryotic Tn5 transposase system (Nextera DNA library kit, Illumina, FC-121–1030). Transposed DNA was then purified on Diapure columns (Diagenode Cat# C03040001) ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Libraries were constructed by following a previous protocol that optimizes reagent usage for microbial genomes [85] with the Nextera DNA Library Preparation Kit (Illumina, FC-121-1030) and sequenced on an Illumina HiSeq 2500 (UNH ...
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bioRxiv - Developmental Biology 2023Quote: ... and transferred to 3.0 μl Tn5 transposome mixture (0.25 μl Tn5 transposome, 2.5 μl tagmentation buffer, Illumina, FC-121-1030; 0.25 μl H2O). The samples were incubated in 37 °C water bath for 30 min ...
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bioRxiv - Developmental Biology 2023Quote: ... The cDNA was then amplified using 10 cycles and subsequently (0.33 ng) used to construct a pool of uniquely indexed samples using the Nextera XT kit (Illumina, Cat# FC-131-1096). Finally ...
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bioRxiv - Immunology 2023Quote: ... Nuclei were resuspended in the Nextera transposition reaction mix (25 μL 2x TD Buffer, 2.5 μL Nextera Tn5 transposase (Illumina Cat #FC-121-1030), and 22.5 μL nuclease free H2O ...
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bioRxiv - Developmental Biology 2024Quote: ... Approximately 1 ng of cDNA per cell was transformed into a single-cell library using the Nextera XT DNA Library prep kit (Illumina FC-131-1096) and following the manufacturer’s instructions with slight modifications ...
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bioRxiv - Genomics 2024Quote: ... and the final sequencing library was amplified utilizing the KAPA HiFi HotStart Uracil+ ReadyMix PCR Kit along with index primers from the Nextera XT Index Kit (Illumina, FC-131-1001). The amplification protocol involved an initial step at 98°C for 45 seconds ...
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bioRxiv - Microbiology 2024Quote: ... using the standard procedure recommended by the manufacturer with a 0.5% addition of the PhiX control library (Illumina, cat. no. FC-110-3001).
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bioRxiv - Microbiology 2024Quote: ... Sequencing was performed using Pair-end 2×100 cycle mode on the Illumina NovaSeq 6000 system (NovaSeq 6000 S1 Reagent Kit v1.5 200 cycles Illumina, cat. no. 20028317; 0.5% of the PhiX control library, Illumina, cat. no. FC-110-3001) and the standard clustering procedure.
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bioRxiv - Microbiology 2023Quote: ... The cDNA libraries were then fragmented and amplified for sequencing using the Illumina Nextera XT DNA Library Preparation kit (Illumina, FC-131-1096) using custom primers that enabled the specific amplification of only the 3’ ends ...
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bioRxiv - Immunology 2024Quote: ... paired-end libraries were constructed from 1 µg of the initial genomic material using the TruSeq DNA v2 Sample Prep Kit (Illumina, #FC-121-2001) as per the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... and R2-Uni13 (5’-GTC TCG TGG GCT CGG AGA TGT GTA TAA GAG ACA GAG TAG AAA CAA GG-3’) (adaptor and barcode oligonucleotide sequences from Illumina, Inc., San Diego, CA, USA). Annealing and extension steps were performed for 30 s at 55oC and 7 m at 72oC ...
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bioRxiv - Genomics 2020Quote: ... The remaining 1μl re-dissolved contents were tagmented using 0.6 μl TD Tagmentation buffer and 0.3 μl ATM Tagmentation enzyme from Nextera XT DNA Library Prep Kit (Illumina, catalog no. FC-121-1030) for 5min at 55 °C ...
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bioRxiv - Genomics 2020Quote: ... The pool was denatured with sodium hydroxide according to Illumina recommendation and spiked with 5% PhiX (PhiX control v3 Illumina Catalogue FC-110-3001) before loading (Fig 1).
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bioRxiv - Genomics 2019Quote: ... Genomic DNA were pooled in equimolar ratio according to sex and Pool-seq libraries were generated using the Truseq nano DNA sample prep kit (Illumina, ref. FC-121-4001) following the manufacturer’s instructions ...
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bioRxiv - Genomics 2020Quote: ... according to the instructions for qChIP-Seq and paired-end sequencing (2×75nt) was performed on a NextSeq 500/550 using a HighOutput v2 Kit for 150 cycles (Illumina #FC-404-2002, discontinued).
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bioRxiv - Genomics 2020Quote: ... according to the manufacturer’s instructions and paired-end sequencing (2 × 75 bp) was performed on a NextSeq 500/550 using a HighOutput v2 Kit for 150 cycles (Illumina #FC-404-2002, discontinued).
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bioRxiv - Genetics 2021Quote: Tagmentation plates were prepared by the combination of 420 uL of 1400 nuclei/uL solution with 540 uL 2X TD Buffer (Nextera XT Kit, Illumina Inc. FC-131-1024). From this mixture ...
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bioRxiv - Immunology 2021Quote: ... prior to pooling and sequencing on an Illumina NextSeq 500 sequencer utilizing a BRB-seq custom primer and the High Output v2 kit (75 cycles) (Illumina, Cat. #FC-404-2005). The sequencing configuration is as follows ...
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bioRxiv - Neuroscience 2019Quote: ... and 1% Input were generated for NDC and PSEN1M146L conditions at the UC San Diego IGM sequencing core using Illumina TruSeq LT adaptors (Illumina Cat. FC-121-2001). Sequencing was performed on an Illumina HiSeq4000 generating Single-Read ...
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bioRxiv - Cancer Biology 2020Quote: ... and poly-A sequencing libraries (HE2.1 and TIVE cells) were generated using the TruSeq RNA Sample Prep Kit (Illumina, catalog no. FC-122-1001).