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Citations for Illumina :
751 - 800 of 928 citations for Recombinant Mouse Icam2 Fc tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2019Quote: ... Genomic DNA were pooled in equimolar ratio according to sex and Pool-seq libraries were generated using the Truseq nano DNA sample prep kit (Illumina, ref. FC-121-4001) following the manufacturer’s instructions ...
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bioRxiv - Genomics 2020Quote: ... according to the instructions for qChIP-Seq and paired-end sequencing (2×75nt) was performed on a NextSeq 500/550 using a HighOutput v2 Kit for 150 cycles (Illumina #FC-404-2002, discontinued).
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bioRxiv - Genomics 2020Quote: ... according to the manufacturer’s instructions and paired-end sequencing (2 × 75 bp) was performed on a NextSeq 500/550 using a HighOutput v2 Kit for 150 cycles (Illumina #FC-404-2002, discontinued).
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bioRxiv - Genetics 2021Quote: Tagmentation plates were prepared by the combination of 420 uL of 1400 nuclei/uL solution with 540 uL 2X TD Buffer (Nextera XT Kit, Illumina Inc. FC-131-1024). From this mixture ...
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bioRxiv - Immunology 2021Quote: ... prior to pooling and sequencing on an Illumina NextSeq 500 sequencer utilizing a BRB-seq custom primer and the High Output v2 kit (75 cycles) (Illumina, Cat. #FC-404-2005). The sequencing configuration is as follows ...
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bioRxiv - Neuroscience 2019Quote: ... and 1% Input were generated for NDC and PSEN1M146L conditions at the UC San Diego IGM sequencing core using Illumina TruSeq LT adaptors (Illumina Cat. FC-121-2001). Sequencing was performed on an Illumina HiSeq4000 generating Single-Read ...
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bioRxiv - Cancer Biology 2020Quote: ... and poly-A sequencing libraries (HE2.1 and TIVE cells) were generated using the TruSeq RNA Sample Prep Kit (Illumina, catalog no. FC-122-1001).
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bioRxiv - Cancer Biology 2020Quote: ... The tagmented and multiplexed cDNA libraries were sequenced in Nextseq 500 using Nextseq 500 High Output v.2 kit (75 cycles, Illumina Cat# FC-404-2005) following Macosko et al ...
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bioRxiv - Cancer Biology 2022Quote: ... 50.000 MDA-MB-231 cells infected with shControl (C) or shLOXL2 (KD) were collected and treated with transposase Tn5 (Nextera Tn5 Transposase, Illumina Cat #FC-121-1030). DNA was purified using AMPure XP beads to remove big fragments (0.5x beads ...
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bioRxiv - Cell Biology 2019Quote: ... Libraries were barcoded and sequenced on a NextSeq Series Sequencing System (HUJI Center for Genomic Technologies) using Illumina flow cell (Illumina 500 NextSeq High Output v2 Kit, FC-404-2005; Illumina, San Diego, CA, USA). The sequencing data were uploaded to the usegalaxy.org (Afgan et al. ...
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bioRxiv - Cancer Biology 2020Quote: ... Sequencing was performed using the NextSeq 500/550 High-Output v2 (150 cycle) Kit (# FC-404-2002) on the NextSeq 550 platform (Illumina Inc, #SY-415-1002). Libraries were combined in an equimolar pool based on the library quantification results and run across 5 High-Output Flow Cell v2.5.
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bioRxiv - Genomics 2021Quote: ... Illumina Catalogue FC-404-2003) following the Illumina recommended denaturation and loading recommendations which included a 1% PhiX spike (PhiX Control v3 Illumina Catalogue FC-110-3001).
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bioRxiv - Cell Biology 2022Quote: ... an average of 500 pg of amplified cDNA was used to prepare the libraries following Nextera XT DNA kit (Illumina Ref. FC-131-1096). Libraries were multiplexed by 6 on a high-output flow cells ...
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bioRxiv - Genomics 2022Quote: ... Illumina Catalogue FC-404-2003) following the Illumina recommended denaturation and loading recommendations which included a 1 % PhiX spike in (PhiX Control v3 Illumina Catalogue FC-110-3001). Data was uploaded to Basespace (www.basespace.illumina.com ...
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bioRxiv - Microbiology 2023Quote: ... 5 µl of this reaction was used in a subsequent round of amplification using BC amp set 3 primers to add Illumina adapters to the sequences followed by amplification using the Nextera XT DNA Library Preparation Kit (Illumina cat. FC-131-1096) to add 5’ and 3’ indices to the library ...
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bioRxiv - Systems Biology 2022Quote: ... single reads and 7 bases index read on an Illumina HiSeq300 instrument using the TruSeq SBS Kit HS-v3 (50-cycle) (FC-401-3002, Illumina Inc, San Diego, CA).
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bioRxiv - Evolutionary Biology 2022Quote: ... sequencing library with a peak insert size of 535 bp was constructed using 200 ng of genomic DNA with a TruSeq Nano LT library preparation kit (Cat # FC-121-4002, Illumina, San Diego, CA, USA) according to the kit supplier’s instructions ...
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bioRxiv - Evolutionary Biology 2022Quote: ... library with a peak span size of 1450 bp and a mean span size of 1027 bp was prepared from 570 ng of genomic DNA by tagmentation using an MP library preparation kit (Cat # FC-132-1001, Illumina, San Diego, CA, USA), without size selection ...
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bioRxiv - Cancer Biology 2023Quote: ... cDNA samples were then diluted to 0.2-0.3 ng per uL and 1 ng of cDNA was carried over into library preparation using the Nextera XT DNA Library Preparation Kit (Illumina, cat. # FC-131-1096) following the manufacturer’s protocol ...
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bioRxiv - Physiology 2023Quote: ... A second round of PCR was performed with 50 ng DNA input for 8 cycles to attach Nextera XT indices and adapters using the Illumina Nextera XT Index Kit (Illumina, Catalog #FC-131-200x) and Phusion HF PCR master mix (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2023Quote: 0.5×105 MIA PaCa-2 cells for each treated condition/replicate infected was collected and treated with transposase Tn5 (Nextera Tn5 Transposase; Illumina Cat #FC-121-1030). DNA was purified using AMPure XP beads to remove large fragments (0.5 × beads ...
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bioRxiv - Immunology 2023Quote: ... The library was then denatured and diluted to 1.2 pM according to standard Illumina’s protocols (for 4 nM libraries) and was spiked with 22% of 20 pM PhiX control (Illumina, cat. no: FC-110-3001). The 1.2 pM library was sequenced on the NextSeq using the NextSeq 500/550 Mid Output Kit v2.5 (300 Cycles ...
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bioRxiv - Immunology 2023Quote: ... Quantified library pool was diluted to 1nM and further diluted as per protocol and sequenced on Illumina MiniSeq (Illumina, Cat. No. FC-420-1001) to check for quality of reads ...
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bioRxiv - Developmental Biology 2023Quote: ... ATAC-seq libraries were generated as described previously (Buenrostro, Giresi et al. 2013) using the Nextera XT DNA Library preparation kit (Illumina catalog: FC-131-1024). Sequencing was performed on an Illumina HiSeq 2500 with 30 - 40 million 75bp paired-end reads ...
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bioRxiv - Plant Biology 2024Quote: ... RNA-sequencing (RNA-seq) libraries for triple replicates of both Col-0 and mac3a/mac3b were prepared utilizing the Illumina TruSeq toolkit (Illumina, Cat# FC-122-1001). Paired-end reads were processed with Trimmomatic (Bolger et al. ...
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bioRxiv - Microbiology 2023Quote: ... Libraries prepared by targeted amplification of the variable V3-V4 regions of the bacterial 16S rRNA gene and attachment of Nextera XT indexes (Illumina Catalog #FC-131-2001) with PrimeStar Taq DNA Polymerase (Takara ...
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bioRxiv - Cell Biology 2024Quote: ... 8 bases for index 1 and 8 bases for index 2) on NextSeq 500 using the NextSeq 500 High Output Kit 75-cycles (Illumina, Cat# FC-404-1005). Flow lanes were loaded at 1.8pM ...
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bioRxiv - Cell Biology 2024Quote: ... 8 bases for index 1 and 8 bases for index 2) on NextSeq 500 using the NextSeq 500 High Output Kit 75-cycles (Illumina, Cat# FC-404-1005). Flow lanes were loaded at 1.8pM ...
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bioRxiv - Genetics 2023Quote: ... The mm10 mouse genome manifest from Illumina was used as reference ...
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bioRxiv - Evolutionary Biology 2020Quote: ... The good quality samples were used to prepare gDNA libraries for all four pooled samples with TruSeq® Nano DNA Library Prep from Illumina (Catalog#FC-121-9010DOC). The libraries were further used for paired-end sequencing with HiSeq2000 (Illumina ...
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bioRxiv - Genomics 2020Quote: ... We run the final pooled library at a concentration of 1.8 pM on a mid-output flow cell (NSQ® 500 Mid Output KT v2 300 cycles; Illumina Catalogue No. FC-404-2003) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... pooled and sequenced with 75 basepair single reads to a depth ranging from 13 to 55 million reads per sample on a Illumina NextSeq 500 using a High Output 75 cycles kit (Illumina, catalog number: FC-404-2005).
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bioRxiv - Genomics 2019Quote: ... We constructed a genomic DNA library using a modified version of the Nextera DNA Library Prep kit (#FC-121-1031, Illumina, Inc., San Diego, CA, USA) meant to conserve reagents [71] ...
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bioRxiv - Molecular Biology 2020Quote: ... the purified sample was used as the template for barcoding with Nextera XT Index Kit v2 (FC-131-1002 - Illumina Inc, San Diego, CA, USA). The finished libraries were normalized to 4 nM and pooled ...
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bioRxiv - Cancer Biology 2019Quote: All libraries were sequenced using the Illumina HiSeq 2000/2500 system with TruSeq SBS Kit v3 - HS (200-cycles) reagents (Illumina, Part no. FC-401-3001), to generate paired-end 101 bp reads.
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bioRxiv - Evolutionary Biology 2020Quote: ... The libraries were sequenced after on-board cluster generation for 80 cycles using 1× HiSeq Rapid SBS Kit v2 (Illumina, Cat. No. FC-402-4022) and HiSeq SR Rapid Cluster Kit v2 (Illumina ...
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bioRxiv - Cell Biology 2022Quote: ... Tagmentation products were amplified by PCR by adding 1.25 µl of each N and S primers (Illumina Nextera XT 96-index kit FC-131- 1002) and 3.75 µl of NPM solution and using the following thermocycler settings ...
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bioRxiv - Cancer Biology 2023Quote: ... we dispensed 600 nL of tagmentation reaction mix containing Tagmentation DNA buffer (TD) and Amplicon Tagment Mix (ATM) at 2:1 v/v ratio (Nextera kit, Illumina, cat. no. FC-131-1096) into each well and performed tagmentation at 55° C for 5 min followed by hold at 4° C in a PCR thermocycler ...
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bioRxiv - Immunology 2023Quote: ... Up to 200pg cDNAs were used to generate the dual index Illumina libraries using Nextera XT DNA Library Prep Kit (Illumina Cat No. FC-131-1096) and Nextera XT Index Kit v2 Set A (Illumina ...
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bioRxiv - Genomics 2020Quote: ... The pooled library was run at a final concentration of 1.8 pM on an Illumina NextSeq500 instrument using a mid-output flow cell (NSQ® 500 Mid Output KT v2 300 cycles; Illumina Catalogue No. FC-404-2003) following the Illumina recommended denaturation and loading parameters ...
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bioRxiv - Genomics 2020Quote: ... and mouse libraries on a NextSeq 500 (Illumina).
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bioRxiv - Neuroscience 2022Quote: ... Ribo-Zero Gold (Human/Mouse/Rat) Kit (Illumina; NEBNext® rRNA Depletion Kit (Human/Mouse/Rat)(E6350 ...
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bioRxiv - Microbiology 2023Quote: ... ‘human/mouse’ and ‘bacteria’ Ribo-Zero kits (Illumina). RNA quality and concentration were verified by BioAnalyzer (Agilent Technologies ...
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bioRxiv - Neuroscience 2020Quote: ... the nuclei-containing pellet was resuspended with 50 µl of the transposase reaction mix which contained 25 µl of the 2xTD reaction buffer and 2.5 µl of Tn5 Transposase (Nextera DNA Library Preparation Kit, Illumina, FC-121-1030; note: these reagents are now available in the Illumina Tagment DNA TDE1 Enzyme and Buffer Kit). Transposition was performed for 30 minutes at 37°C after which the transposed DNA was purified using MinElute PCR Purification Kit (Qiagen ...
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bioRxiv - Microbiology 2023Quote: ... half of the samples had libraries prepared using the Accel-NGS kit (Washtenaw County, Michigan) and the other half using the Nextera XT kit (Illumina, San Diego, CA; cat# FC-131-1024). The 40 virome libraries were sequenced with Illumina HiSeq-1TB ...
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bioRxiv - Neuroscience 2023Quote: ... Mouse GRCm38/mm10 genome annotation was accessed from Illumina’s iGenomes repository (available at https://support.illumina.com/sequencing/sequencing_software/igenome.html ...
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bioRxiv - Cancer Biology 2019Quote: ... and TruSeq Stranded Total RNA Human/Mouse/Rat (Illumina, 20020596) with 100 ng of input and 13 PCR cycles ...
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bioRxiv - Genetics 2019Quote: ... the Ribo-Zero rRNA Removal kit (Human/Mouse/Rat, Illumina) was employed to deplete ribosomal RNA from 20 µg of total human or mouse brain RNA according to the manufacture’s instruction ...
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bioRxiv - Molecular Biology 2022Quote: ... The Ribo-Zero rRNA Removal Kit (Illumina human/mouse/rat) was applied to remove the rRNAs ...
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bioRxiv - Cell Biology 2023Quote: ... Infinium Mouse Methylation BeadChip (Illumina, Inc., San Diego, CA, USA) arrays were used to profile DNA methylation genome wide ...