Labshake search
Citations for Illumina :
7601 - 7650 of 9681 citations for ssc mir 758 Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... and used for metagenomic library preparation with TruSeq® Nano DNA LT Sample Prep Kit (Illumina, San Diego, CA, USA). The high-throughput sequencing was performed on an Illumina Miseq platform (2 × 300bp).
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bioRxiv - Microbiology 2021Quote: ... Genomic junctions of each secondary gene replacement were amplified by first Tagmenting the DNA with TDE1 from the Nextera DNA Sample Preparation Kit (Illumina) and subsequently performing PCR with primers specific for the Nextera transposon (2A-R ...
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bioRxiv - Microbiology 2021Quote: ... Samples were sequenced using a MiSeq sequencing system and MiSeq V3 (2 × 300 bp) reagent kits (Illumina, San Diego, CA).
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bioRxiv - Evolutionary Biology 2021Quote: ... and then sequenced as 101 paired-end reads on the HiSeq2500 platform (using the HiSeq® SBS Kit v4, Illumina) at the Genomics Facility Basel of the University of Basel and the Department of Biosystems Science and Engineering of the ETH Zürich.
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bioRxiv - Physiology 2021Quote: ... Samples were subject to another bead clean up prior to A-tailing and ligation of adapters as per kit instructions (Illumina). Following a third bead clean-up ...
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bioRxiv - Microbiology 2020Quote: ... Samples were multiplexed on a single MiSeq flowcell using Nextera XT barcodes and normalised using the bead-based normalisation kit (Illumina). Sequencing of 41 pooled genomes was performed simultaneously at Makerere University ...
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bioRxiv - Developmental Biology 2020Quote: ... Clustering of the index-coded samples was assessed on a cBot Cluster Generation System using the TruSeq SR Cluster Kit v3-cBot-HS (Illumina) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2019Quote: Transcriptome sequencing cDNA libraries of macrophage RNA were prepared using a stranded mRNA polyA selection (Truseq stranded mRNA kit, Illumina). For each sample ...
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bioRxiv - Cancer Biology 2019Quote: ... RNA sequencing library preparation was performed with the High Throughput TruSeq Stranded mRNA Library Prep Kit (Illumina, # RS-122-2103) following the manufacturer’s protocol at half reaction volume ...
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bioRxiv - Plant Biology 2020Quote: ... The clustering of the index-coded samples was performed on a cBot Cluster Generation System using HiSeq PE Cluster Kit cBot-HS (Illumina) according to the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2020Quote: ... The samples were sequenced by single-end reads (75 bp) following the protocol of the NextSeq 500 High Output kit V2 (75 cycles) (Illumina) on an Illumina NextSeq 500 system.
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bioRxiv - Cancer Biology 2020Quote: ... libraries for mRNA sequencing were prepared using the QuantSeq 3’ mRNA FWD kit (Lexogen) prior to sequencing on the Nextseq500 (High output, SR75, Illumina). On average 7.0 ± 1.9M high-quality reads were generated per samples ...
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bioRxiv - Evolutionary Biology 2020Quote: ... The RNA-seq library of this sample was prepared using the TruSeq Stranded mRNA LT Sample Prep kit (RS-122-2101, Illumina) with 500 ng total RNA as input ...
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bioRxiv - Neuroscience 2020Quote: ... Sequencing was performed on Illumina HiSeq 4000 paired-end at 2 × 126 bp using the TruSeq SBS kit v4-HS (Illumina).
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bioRxiv - Genomics 2021Quote: Total RNA and nascent RNA samples were subjected to strand-specific RNA-seq library preparation with the KAPA RNA HyperPrep kit with RiboErase (Illumina), which included 1st and 2nd strand synthesis and ribosomal RNA depletion ...
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bioRxiv - Immunology 2021Quote: ... The cDNA was enriched for V(D)J cDNA using the Human B cell Chromium Single Cell V(D)J Enrichment Kit (Cat#1000016) followed by library construction to add the priming sites used by Illumina sequencers ...
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bioRxiv - Plant Biology 2021Quote: ... RNA-seq libraries were prepared from total RNA after rRNA depletion with the Ribo-Zero rRNA Removal Kit (Plant Leaf) (Illumina) using the NEXTflex Rapid Directional qRNA-Seq Kit (Bioo Scientific) ...
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bioRxiv - Microbiology 2021Quote: Sequencing libraries were made according to the Illumina standardized protocol using the Nextera XT DNA sample preparation kit (Illumina Inc.). All DNA samples were quantitated by Qubit HS (ThermoFisher Scientific ...
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bioRxiv - Cancer Biology 2021Quote: ... Approximately 500 ng of total RNA was used as input for library preparation using TruSeq RNA Sample Preparation Kit v2 (Illumina).
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bioRxiv - Immunology 2021Quote: ... cDNA concentrations were normalised and 0.125 ng of each cDNA was used in a quarter volume of a Nextera XT DNA library preparation kit (Illumina), following pooling of equal volumes from each well and purification with 0.6x AMPure XP SPRI beads ...
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bioRxiv - Genetics 2020Quote: ... Transposition reaction was performed according the manufacturer’s protocol for Nextra Tn5 transpoase Nextra kit (Illumina, Cat. No.: FC-121-1030). Transposed DNA fragments were purified by Qiagen MiniElute PCR purification kit (Qiagen) ...
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bioRxiv - Immunology 2020Quote: ... Two hundred ng of total RNA were used to generate libraries using the TruSeq Stranded mRNA Sample Preparation Kit (Illumina). Single-end reads of 100bp for each sample were produced with Illumina’s HiSeq2500v4 instrument ...
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bioRxiv - Immunology 2020Quote: ... Single cell RNA libraries were prepared according to the 10x Genomics Chromium Single Cell 3’ Reagent Kits v2 User Guide and sequenced on a HiSeq4000 (Illumina).
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bioRxiv - Genomics 2019Quote: ... was isolated from 1 μg of total RNA and 12 RNA-Seq libraries prepared using the TruSeq RNA Sample Preparation Kit (Illumina). Libraries were sequenced on an Illumina HiSeq 2000 platform ...
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bioRxiv - Genetics 2019Quote: ... 100ng of genomic DNA was used to prepare Bisulfite libraries with the TruSeq Nano kit (Illumina, San Diego, CA, USA) according to the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2019Quote: ... and mate-pair libraries of 3 kb and 6 kb insert sizes were prepared using the Nextera Mate Pair Sample Preparation Kit (cat. No. FC-132-1001, Illumina). We then assessed library quality using the HS DNA Kit (Agilent ...
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bioRxiv - Evolutionary Biology 2019Quote: ... Individual cDNA libraries were prepared for each individual using the TruSeq Stranded mRNA Sample Preparation kit (Illumina, San Diego, CA) in combination with a Poly-A selection step ...
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bioRxiv - Cancer Biology 2019Quote: ... The multiplexed RNA-seq library was normalized to 4nM with Suspension Buffer (provided in Nextera XT kit) to be sequenced on the NextSeq 500 Sequencer (Illumina).
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bioRxiv - Microbiology 2019Quote: ... The duplicated cDNA libraries for each of the ten host-location combinations were obtained using the Nextera XT library preparation kit and sequenced by Illumina HiSeq 2500 fast mode ...
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bioRxiv - Physiology 2019Quote: ... The input was quantified by using Qubit RNA BR Assay Kit and RNA-seq was conducted using NextSeq Series RNA-seq Solution (Illumina) at the Center of Genomics and Bioinformatics ...
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bioRxiv - Immunology 2019Quote: ... Amplicon mixtures corresponding to 10:1 ratios of heavy chain + lambda: kappa were submitted for 300 forward × 250 reverse sequencing with MiSeq v3 kits (Illumina) at the Chan Zuckerberg Biohub Genomics Center ...
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bioRxiv - Microbiology 2019Quote: ... bacterial genomic DNA was prepared for sequencing on the Illumina MiSeq by the Yale Center for Genomic Analysis using the TruSeq DNA LT Sample Prep Kit (Illumina). Purified libraries were barcoded ...
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bioRxiv - Microbiology 2019Quote: ... The quality of the DNA samples was verified before DNA libraries were prepared using a Nextera XT Library Prep kit (Illumina). Paired-end sequencing was performed using a NextSeq 500 sequencer (Illumina ...
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bioRxiv - Genomics 2019Quote: ... (2) six cases prepared with the Nextera Mate-pair Library Construction Kit (hereafter called Nextera, Illumina, San Diego, CA, USA) released by the 1000 Genomes Project 25 were used for comparison.
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bioRxiv - Microbiology 2019Quote: ... and paired-end sequences (2 x 250-bp) were generated on an Illumina MiSeq instrument with a 500-cycle MiSeq Reagent v2 kit (Illumina). Library preparation and sequencing was done at the Genetic Diversity Center Zurich (GDC) ...
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bioRxiv - Neuroscience 2019Quote: ... polyadenylated RNA was purified from the total RNA samples using Oligo dT conjugated magnetic beads and prepared for single-end sequencing according to the Illumina TruSeq RNA Sample Preparation Kit v2 (# RS-122-2001, Illumina). The libraries were sequenced for paired-end 75 cycles using the TruSeq SBS kit on NextSeq 500 system ...
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bioRxiv - Genomics 2019Quote: ... Libraries were quality-controlled prior to sequencing based on Agilent 2100 Bioanalyzer profiles and normalized using the KAPA Library Quantification Kit (Illumina). Libraries were sequenced on the Illumina NextSeq500 platform using paired-end 43bp mode with 15 million reads per sample for pooled libraries and paired-end 75bp mode with 1-3 million reads per sample for single-cell and single-nucleus libraries.
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bioRxiv - Neuroscience 2019Quote: ... cells were pooled and sequenced in an Illumina NextSeq500 instrument using 2 × 75 paired end reads on a NextSeq high-output kit (Illumina). After de-multiplexing the raw reads to single-cell datasets ...
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bioRxiv - Cancer Biology 2019Quote: ... cDNA libraries were constructed using the SMARTer universal low input RNA kit (Clonetech) and sequenced using a HiSeq 2500 Sequencer (Illumina) 100bp SR.
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bioRxiv - Systems Biology 2019Quote: ... Library preparation was performed using the TruSeq Stranded mRNA LT Sample Prep Kit (Illumina USA, Cat. No. RS-122-2101) and all samples were sequenced in single end mode in one run on an Illumina NextSeq (NS500720 ...
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bioRxiv - Immunology 2019Quote: ... cDNA was quantified using Biotium AccuClear High Sensitivity DNA quantification reagent and normalised to 1 ng/µl in a total volume of 500 nl before library preparation using Nextera XT DNA Sample Preparation Kit (Illumina). 125 nl of in-house index adapters (Integrated DNA Technologies) ...
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bioRxiv - Evolutionary Biology 2019Quote: ... The index-coded samples were clustered with a cBot Cluster Generation System using a TruSeq PE Cluster Kit v3-cBot-HS (Illumina), following the manufacturer’s instructions ...
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bioRxiv - Genomics 2019Quote: ... Sequencing libraries of A and B containing 6 bp indexes were prepared using the TruSeq RNA sample prep kit (Illumina) following a modification of the manufacturer’s protocol ...
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bioRxiv - Genomics 2021Quote: ... cells and CD14+ monocytes were generated using the SureCell WTA 3′ Library Prep Kit for the ddSEQ System (20014280, Illumina) with the following modifications ...
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bioRxiv - Genomics 2021Quote: ... RNA libraries were prepared for sequencing by the Cancer Genomics Shared Resource (Wake Forest School of Medicine, Winston-Salem, NC) using the TruSeq-stranded total RNA kit (Illumina), which includes a ribosomal depletion step ...
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bioRxiv - Genomics 2021Quote: ... The samples were amplified for 5-8 cycles as determined by qPCR for Illumina sequencing using Nextera library preparation kit (Illumina) and samples were paired-end sequenced on HiSeq4000 ...
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bioRxiv - Genetics 2020Quote: ... Ribosomal RNA was subtracted by hybridization from total RNA samples using the RiboZero Magnetic Gold H/M/R Kit (Illumina) and the rRNA-subtracted samples were quantified with a Qubit 2.0 (RNA HS Kit ...
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bioRxiv - Genetics 2021Quote: ... one 150 bp paired end (350 bp insert size) Illumina library was prepared from the same DNA (NEBNext Ultra II DNA Library Prep Kit for Illumina) and sequenced to approximately 140 fold coverage (24.6 Gb ...
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bioRxiv - Evolutionary Biology 2020Quote: ... 250 ng of purified genomic DNA was used from each sample for library preparation using the Nextera DNA Flex Library Prep Kit (Illumina) per the manufacturer’s protocols ...
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Nonsense Mediated RNA Decay Is a Unique Vulnerability of Cancer Cells with SF3B1 and U2AF1 MutationsbioRxiv - Cell Biology 2021Quote: ... RNA-sequencing libraries were then prepared from RNA isolated from the bulk mutant AML bone marrow cells and the normal CD34+ bone marrow cells using TruSeq Stranded Total RNA kit (Illumina) described above ...