Labshake search
Citations for Illumina :
7551 - 7600 of 9193 citations for Mouse CXCL2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... M9-glycerol or at distinct phases of infection) was subjected to tagmentation using the Nextera DNA Library Prep Kit (Illumina). The DNA was mixed with 10 μL of 2x TD Buffer and 5 μL of Tagment DNA Enzyme TDE1 (diluted 1/50 in 0.5x TE buffer and 50 % (v/v ...
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bioRxiv - Molecular Biology 2023Quote: ... Purified libraries were controlled by capillary electrophoresis (LabChip - Perkin) and quantitated by qPCR (KAPA Library Quantification Kits Illumina Platforms - Roche). Pooled libraries (12 pM ...
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bioRxiv - Immunology 2023Quote: ... Whole-genome sequencing (WGS) was performed using the Illumina NovaSeq 6000 with the S4 reagent kit (300 cycles) (Illumina, USA) with 2 × 150 bp paired-end reads ...
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bioRxiv - Genomics 2023Quote: ... we prepared stranded cDNA libraries for 909 samples from 695 sepsis patients using NEB Ultra II LIbrary Prep kits (Illumina) with NEBNext Poly(A ...
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bioRxiv - Genomics 2023Quote: ... 1 ng of the purified fragment was then used to prepare sequencing libraries with the Nextera XT DNA Library Preparation Kit (Illumina, Cat # FC-131-2002 or Cat # FC-131-1024 ...
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bioRxiv - Genomics 2023Quote: ... The sequencing libraries were generated on the streptavidin C1 beads using Illumina Tagment DNA Enzyme and Buffer kit (Illumina, 20034198) and run on the Illumina Novaseq S4 flowcell.
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bioRxiv - Microbiology 2023Quote: ... Libraries were constructed using the Illumina Stranded Total RNA library preparation protocol and rRNA was removed using the Ribo-Zero Plus rRNA depletion kit (Illumina). Libraries were sequenced on the Illumina NovaSeq 6000 platform (paired-end ...
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bioRxiv - Microbiology 2023Quote: ... Libraries were sequenced on the Illumina NextSeq500 platform with paired ends for 2 x 150-base pair reads using a Mid-output cartridge Kit v2.5 (Illumina, 20024905). bcl2fastq software was used to demultiplex and compress bcl files into fastq (gz ...
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bioRxiv - Neuroscience 2023Quote: ... after which onboard clonal clustering of the patterned flow cell was performed using a NovaSeq 6000 S1 Reagent Kit v1.5 (200 cycle) (Illumina 20028318). A 2x101 cycle sequencing profile with dual index reads was completed using the following sequence profile ...
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bioRxiv - Molecular Biology 2023Quote: ... 11200g for five minutes prior to dividing the lysate for either nuclease foot-printing with RNaseI for generation of the ribosome profiling libraries or total RNA extraction followed by removal of rRNA with RiboZero (plant leaf kit, Illumina) for the corresponding RNA-Seq library.
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bioRxiv - Microbiology 2023Quote: ... The libraries were sequenced in a 250-bp paired-end run using the MiSeq Reagent Kit v2 (Illumina; 500 cycles).
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bioRxiv - Neuroscience 2023Quote: ... The cDNA samples that passed quality control were used to generate sequencing libraries using the Nextera XT DNA Library Preparation Kit (Illumina), following the user guide ...
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bioRxiv - Biochemistry 2023Quote: ... Libraries were then performed by PCR with a library preparation kit (Vazyme, TD501) and sequencing was done using the Novaseq system (Illumina) according to the manufacturer’s protocol.
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bioRxiv - Cancer Biology 2023Quote: ... Libraries were prepared by the Sequencing Facility at NCI-Leidos using the TruSeq Stranded mRNA Kit (Illumina, San Diego, CA) and sequenced paired-end on NextSeq (Illumina ...
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bioRxiv - Cancer Biology 2023Quote: ... The 100-cycle paired end sequencing run was performed using a NovaSeq 6000 S1 Reagent Kit v1.5 200 cycle (Illumina 20028318). Sequence data was converted to fastq files ...
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bioRxiv - Cancer Biology 2023Quote: ... RNA samples were sequenced using Truseq Stranded Total RNA Library Prep Kit and Illumina HiSeq4000 PE100 (Illumina, San Diego, CA) by the Genome Quebec Innovation Centre ...
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bioRxiv - Bioengineering 2023Quote: ... pair-end single index sequencing parameters were adjusted to 101/6/0/86 (as opposed to 74/6/0/86) to effectively utilize the capabilities of the NovaSeq 200 cycle reagent kit (Illumina). To demultiplex each sample and to generate the matrix of transcript counts in each cell ...
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bioRxiv - Evolutionary Biology 2023Quote: ... was performed on two lanes of an Illumina HiSeq3000 using a paired-end read length of 2×150 bp with the Illumina HiSeq3000 sequencing kits (Illumina) at the GeT-PlaGe core facility ...
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bioRxiv - Cancer Biology 2023Quote: Bead-purified cDNA pools were used for tagmentation-based library preparation with a Nextera XT DNA Library Preparation Kit (Illumina) using a custom PCR amplification strategy to generate 3’ biased libraries containing oligodT cell barcodes as previously published,60 with some modifications ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were processed according to Chromium 3’ Gene Expression V3 Kit (10X Genomics) using the manufacturer’s guidelines followed by sequencing on an S1 Novaseq chip (Illumina Inc.) at the Genomics Core of Icahn School of Medicine at Mount Sinai.
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bioRxiv - Cancer Biology 2023Quote: ... a total RNA input of 200 ng was used for library preparation using the Illumina Stranded Total RNA Prep with Ribo-Zero Plus Kit (Illumina) and IDT for Illumina RNA UD Indexes Set A ...
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bioRxiv - Cancer Biology 2023Quote: ... 100 ng DNase treated RNA was used to generate ribo-minus treated total RNA stranded libraries using Illumina Stranded Total RNA Prep Ligation with Ribo-Zero Plus kit (Illumina) according to manufactures’ instructions ...
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bioRxiv - Neuroscience 2023Quote: ... 500 ng of RNA was used as an input and libraries were prepared using the TruSeq Stranded mRNA library preparation kit from Illumina. The samples were sequenced on the NovaSeq 600 platform on the NovaSeq S4 300 cycle flowcell to generate 150 bp paired-end reads ...
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bioRxiv - Microbiology 2023Quote: ... then diluted to 150 pM in elution buffer supplied in the library prep kit and loaded to the iSeq100 flow cell for sequencing on iSeq100 (Illumina). After the NGS run ...
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bioRxiv - Cancer Biology 2023Quote: The RNA sequencing was performed by the Yale Center for Genome Analysis (YCGA) after the removal of rRNA from RNA samples using the Ribo-Zero rRNA Removal Kit from Illumina. The RNA libraries were created using the TrueSeq Small RNA Library preparation kit from Illumina ...
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bioRxiv - Molecular Biology 2023Quote: ... Purified multiplex libraries were diluted to 9 nM concentration (calculated based on Qubit dsDNA HS Assay Kit) and sequenced on a NovaSeq 6000 instrument (Illumina) from IntegraGen SA ...
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bioRxiv - Immunology 2023Quote: ... and DNA amplicon products where generated using Ig-specific variable and constant gene primers.87 Samples were indexed (Nextera Index Kit, Illumina), purified ...
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bioRxiv - Cancer Biology 2023Quote: ... The ChIPed and the Input DNA were used to prepare libraries by Kappa Hyperprep kit and sequenced in Nextseq (Illumina).
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bioRxiv - Microbiology 2023Quote: ... Samples were sequenced with the Illumina MiniSeq device using the Illumina MiniSeq Mid output 300 cycles kit (2×150 bp, paired-end, Illumina). Demultiplexing and trimming of the reads was performed using the Local Run Manager (Illumina).
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bioRxiv - Microbiology 2023Quote: ... Library preparations were carried out by Novogene (Beijing, China) using a TruSeq Stranded mRNA kit (Illumina, San Diego, CA, USA), as per manufacturer’s protocol ...
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bioRxiv - Plant Biology 2023Quote: ... The RNAseq library for each sample was prepared with a 1250 ng of total RNA using the TruSeq RNA Sample Preparation kit (Illumina) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2023Quote: RNA-seq libraries were prepared from 1 μg of total RNA using the TruSeq RNA Library Prep Kit v2 (Illumina) with poly(A ...
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bioRxiv - Immunology 2023Quote: ... 200 pg of purified WTA products from each pool were subjected for library construction with custom designed New-P5-SMART-PCR primer using the Nextera XT DNA library preparation kit (Illumina) following the detailed protocol provided by the manufacture ...
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bioRxiv - Microbiology 2023Quote: ... tuberculosis gDNA was fragmented with a Covaris S220 ultrasonicator and a whole genome library was prepared using the TruSeq Nano library preparation kit (Illumina). Library fragments containing Tn junctions were PCR-amplified from the whole genome library using the Tn-specific primer Mariner_1R_TnSeq_noMm and Illumina p7 primer (Table S4) ...
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bioRxiv - Developmental Biology 2024Quote: ... sequencing was performed at The McDermott Center Next Generation Sequencing (NGS) Core at UT Southwestern using TruSeq Stranded mRNA Sample Preparation Kit (Illumina). The reads were then used as reference of single organoid ribo-seq under same condition ...
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bioRxiv - Molecular Biology 2024Quote: ... Libraries were prepared with the VAHTS Stranded mRNA-seq Library Prep Kit (Vazyme Biotech) after poly(A) selection and sequenced on a NextSeq 2000 system (Illumina) or using the TruSeq protocol (Illumina ...
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bioRxiv - Microbiology 2024Quote: ... The following index PCR for both gene libraries was conducted with the Nextera XT index kit v2 (Illumina Inc., USA). The PCR product was purified using the LabopassTM DNA purification kit (Cosmogenetech Inc. ...
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bioRxiv - Molecular Biology 2023Quote: Dual indexed FFPE libraries were constructed with a 500 ng input of total RNA utilizing the TruSeq Stranded Total RNA Library Construction riboGold kit (Illumina). Two pools of five samples each were pooled at an equimolar ratio yielding ∼900ng (Pool 1 ...
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bioRxiv - Microbiology 2023Quote: ... and sequenced as 2 x 150 bp reads on the MiSeq instrument using the MiSeq Reagent Micro kit v2 (Illumina). Raw image files were converted to fastq files using bcl2fastq (v2.20.0.422 ...
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bioRxiv - Bioengineering 2023Quote: ... MiSeq Illumina library preparation was performed using barcoded DNA with the Nextera XT Index kit V2 (Illumina, San Diego, CA), following the manufacturer’s instructions for dual indexing ...
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bioRxiv - Microbiology 2023Quote: ... Total RNA samples were generated and quality was corroborated as described previously.46 rRNA depletion using the Ribo-Zero RNA Plus rRNA Depletion Kit (Illumina), library preparation ...
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bioRxiv - Physiology 2024Quote: ... 150 base-pair paired-end read RNA-seq was performed with HiSeq 3000/4000 PE Cluster Kit (PE-410-1001; Illumina) and HiSeq 3000/4000 SBS Kit (300 Cycles ...
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bioRxiv - Physiology 2024Quote: ... was used for RNA-seq library preparation with a TruSeq Stranded mRNA Library Prep Kit for NeoPrep (NP-202-1001; Illumina) that allows polyA-oligo(dT)-based purification of mRNA ...
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bioRxiv - Plant Biology 2024Quote: ... Strand-specific total RNA-Sequencing libraries were prepared using the TruSeq Stranded Total RNA with Ribo-Zero Plant kit according to the manufacturer’s specifications (Illumina 20020610). ERCC RNA Spike-In Control Mix (Ambion 4456740 ...
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Genetic gradual reduction of OGT activity unveils the essential role of O-GlcNAc in the mouse embryobioRxiv - Developmental Biology 2024Quote: ... Library preparation was performed by EMBL Genomics Core Facility using the TruSeq Stranded mRNA Library Preparation kit according to the manufacturer’s instructions (Illumina #20020594). All libraries were pooled and sequenced in one run (75 bp single-end mode ...
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bioRxiv - Evolutionary Biology 2024Quote: ... RNA was fragmented by hydrolysis for 2 minutes at 94° and cDNA synthesis was performed with random hexamers following the TruSeq Stranded RNA kit protocol (Illumina). To achieve strand specificity ...
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bioRxiv - Immunology 2024Quote: Bulk RNA-Seq: Libraries were generated using a QuantSeq 3’ mRNA-Seq Library Prep Kit (Lexogen) and sequenced using a NovaSeq (Illumina). After adaptor trimming ...
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bioRxiv - Developmental Biology 2024Quote: ... Pooled and size-selected sequencing libraries were sequenced on an Illumina NextSeq 550 instrument using a High Output kit (Illumina) with 38-bp paired-end reads setting.
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bioRxiv - Cell Biology 2024Quote: ... The library preparation and RNA sequencing was performed by Birmingham Genomic centre using Lexogen QuantSeq 3’ mRNA-Seq Library Prep Kit FWD from Illumina. Libraries were quantified using a PicoGreen Quant-iT kit and sized with a D1000 tape ...
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ZNF143 binds DNA and stimulates transcription initiation to activate and repress direct target genesbioRxiv - Genomics 2024Quote: ... 0.1% Tween-20 in PBS) and added 2.5µl of TDE1 Tn5 transposase (Illumina Tagment DNA TDE1 Enzyme and Buffer Kit). We incubated the transposition reaction at 37°C for 30 min ...