Labshake search
Citations for Illumina :
7201 - 7250 of 9484 citations for Cow Fibrinogen Like Protein 1 FGL1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... Libraries were base-pair selected based on Agilent 2100 Bioanalyzer profiles and normalized determined by KAPA Library Quantification Kit (Illumina). The libraries were built from 5 different mice per genotype ...
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bioRxiv - Neuroscience 2021Quote: ... We fragmented the rRNA-depleted RNA samples and processed them into strand-specific cDNA libraries using TruSeq Stranded Total LT Sample Prep Kit (Illumina) and then sequenced them on NextSeq 500 ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Subsequent to second round PCR amplification MiSeq sequencing libraries were generated using Nextera XT DNA Sample Preparation Kits (Illumina, Inc.). Amplicons for each sample were tagged using Nextera XT Index Kits (Illumina ...
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bioRxiv - Genetics 2021Quote: ... and ribosomal RNA was depleted from 5 ug of total RNA using the Ribo-Zero™ Gold Kit (Illumina, Inc.). Depleted mRNA was fragmented and converted to first strand cDNA ...
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bioRxiv - Plant Biology 2021Quote: ... using 10 mM of pooled normalized libraries on the cBOT with a TruSeq PE Cluster Kit v3-cBot-HS (Illumina). Subsequently Illumina HiSeq 4000 sequencing was performed to generate the reads.
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bioRxiv - Cancer Biology 2021Quote: ... and purified RNA was delivered to GENEWIZ (South Plainfield, NJ) for mRNA preparation with the TruSeq RNA V2 kit (Illumina) and 150bp paired-end sequencing on the Illumina HiSeq2500 ...
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bioRxiv - Neuroscience 2021Quote: ... Libraries were prepared using Smart-Seq2 64 and modified for sequencing using the Nextera XT DNA Sample Preparation Kit (Illumina) with 300 pg of cDNA as input material ...
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A tale of two transcriptomic responses in agricultural pests via host defenses and viral replicationbioRxiv - Genomics 2020Quote: ... The rRNA-depleted samples were used for TruSeq Stranded RNA Sample Prep kit to produce 5′ to 3′ strand-specific cDNA libraries (Illumina). A TruSeq SBS sequencing kit version 3 (Illumina ...
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bioRxiv - Developmental Biology 2021Quote: ... rRNA was depleted using Ribo-Zero™ Magnetic Gold Kit for rRNA depletion (Human/Mouse/Rat) (Epicentre for Illumina #MRZG12324) according to manufacturer’s guidelines ...
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bioRxiv - Developmental Biology 2021Quote: ... The libraries were prepared from 300 ng of RNA from each sample using TruSeq Stranded mRNA HT Kit and TruSeq Stranded mRNA Sample Preparation protocol 15031047 (Illumina). Sequencing was performed with NovaSeq 6000 SP Sequencing System (Illumina ...
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bioRxiv - Cell Biology 2019Quote: Libraries of template molecules suitable for strand-specific high-throughput DNA sequencing were created using a TruSeq Stranded Total RNA with Ribo-Zero Gold Prep Kit (RS-122-2301; Illumina) as previously described49 ...
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bioRxiv - Microbiology 2021Quote: ... diluted to a final concentration of 4pM and sequenced using the MiSeq Reagent Kit v3 (600-cycle, paired-end, Illumina) on a MiSeq sequencer (Illumina) ...
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bioRxiv - Genomics 2021Quote: ... RNA sample preparations were performed as described previously [22] using the ARTseq™ and Epicentre’s Ribo-Zero™ kits (Illumina). Briefly ...
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bioRxiv - Genomics 2020Quote: ... and sequenced at the Max Planck Genome Centre (Cologne, Germany) using the HiSeq sequencing kit on a HiSeq2000 cycler (Illumina). Paired-end sequencing was performed with a 100 base pair read length and for each sequencing library ...
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bioRxiv - Genomics 2020Quote: ... DNA pools and from one single individual of each sex were constructed using the Genomic DNA Sample Preparation Kit (Illumina) according to manufacturer’s instructions and sequenced on the Illumina HiSeq2000 platform (Illumina ...
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bioRxiv - Genomics 2020Quote: RNA from a subset of human fetal (n = 3) and mouse (n = 8) cortex tissue samples was prepared with TruSeq Stranded mRNA Sample Prep Kit (Illumina) and subjected to 125bp paired-end sequencing using a HiSeq2500 (Illumina) ...
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bioRxiv - Genomics 2020Quote: ... The cDNA libraries were prepared using Illumina Nextera XT Library Prep Kits and sequenced using the NovaSeq 6000x System from Illumina. NovaSeq S2 100-cycle sequencing was performed at the Broad Institute Genomics Platform.
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bioRxiv - Evolutionary Biology 2021Quote: ... Approximately 30% of Illumina libraries were constructed in-house with the Nextera DNA Library Preparation Kit (Illumina, FC-121-1030) using a protocol based off of previously published protocols51,52 ...
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bioRxiv - Immunology 2020Quote: ... RNA-seq libraries were prepared from both eluate and 10% input mRNA using the TruSeq mRNA library prep kit (Illumina), subjected to quality control (MultiQC) ...
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bioRxiv - Microbiology 2021Quote: ... the two plasmid DNA mixtures were sequenced using an Illumina MiSeq instrument with reagent kit version 3 (2x 300 cycles) as recommended by the manufacturer (Illumina)) ...
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bioRxiv - Microbiology 2021Quote: ... The purified libraries underwent qPCR with KAPA Universal Quant Kit and were subjected for next-generation sequencing on NEXTseq 500 (Illumina).
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bioRxiv - Immunology 2020Quote: ... Libraries were sequenced to obtain more than 20 million 100 × 100 bp paired-end reads (S4 P200 flow cell and sequencing Kit; Illumina) mapping uniquely to mouse mm10 mRNA reference.
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Rbx1 Cullin-Ring ligase E3 and Ube2m neddylation E2 differentially governs the fitness of Treg cellsbioRxiv - Immunology 2020Quote: ... Approximate 1.5pM of the library for each sample was loaded onto a NextSeq 500 system and sequenced using High Output sequencing kits (75× 2bp) (Illumina). After discarding the cells with high mitochondrial gene ...
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bioRxiv - Microbiology 2019Quote: ... Ribosomal RNA (rRNA) was then depleted from each sample (5 µg each) using the bacterial Ribo-Zero rRNA Removal Kit (Illumina). The integrity of the RNA was evaluated using an RNA 6000 Nano LabChip and an Agilent 2100 Bioanalyzer (Agilent Technologies ...
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bioRxiv - Microbiology 2020Quote: Sequencing libraries were prepared from 50 ng of phage genomic DNA using the Nextera DNA Sample Preparation Kit (Illumina, USA) at the University of Cambridge Sequencing Facility ...
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bioRxiv - Microbiology 2020Quote: ... Libraries were prepared using the NEB Next Ultra II kit (New England Biotech, Ipswich, MA) and paired-end sequenced (2×150bp) on a NextSeq system (Illumina). Adaptors were removed using cutadapt v1.10 [28] and reads assembled using SPAdes v3.7.1 [29] ...
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bioRxiv - Microbiology 2020Quote: ... Dual-indexed sequencing libraries were prepared from the lysates using Nextera XT DNA Library Preparation Kit (Illumina FC-131-1096), and sequenced on an Illumina Miseq benchtop sequencer (Illumina ...
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bioRxiv - Neuroscience 2021Quote: ... Sequencing was carried out by running 150 cycles on Illumina HiSeq 2500 using TruSeq Rapid SBS Kit (FC-402-4001, Illumina) according to the manufacturer’s instructions.
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bioRxiv - Microbiology 2020Quote: ... Purified PCR products were combined in equimolar concentrations and sequenced on an Illumina MiSeq (Miseq Reagent Kit V2, 50-cycle; Illumina) using primer PLM49 ...
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bioRxiv - Microbiology 2020Quote: ... gDNA was either submitted for sequencing at the Microbial Genome Sequencing Center at the University of Pittsburgh or prepared for whole genome sequencing in house using the Nextera XT DNA Library Preparation Kit (Illumina). Libraries were sequenced using the NextSeq 550 or MiSeq platforms ...
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bioRxiv - Microbiology 2020Quote: ... One ng of the chromosomal DNA obtained was used in a library preparation using the Nextera XT library preparation kit (Illumina) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... ribosomal RNA (rRNA) was depleted from the total RNA extracts using the Ribo-Zero Gold (human-mouse-rat) kit (Illumina) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... Illumina libraries with an average insert size of 300 bps were constructed from purified genomic DNA using the Nextera XT kit (Illumina) and sequenced on Illumina's NovaSeq platform in a paired end 140 bp read configuration ...
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bioRxiv - Microbiology 2019Quote: ... we performed a second PCR step to add unique dual-index combinations to each sample using the Nextera XT index kit (Illumina). Second-step PCR amplifications were performed in a total volume of 25 μl using 2.5 μl of the PCR products ...
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bioRxiv - Immunology 2019Quote: RNA-seq libraries for CCR6+ ILC3 were prepared with the TruSeq Stranded Total RNA Library Prep Gold Kit (Illumina, 20020598). The sequencing was performed using Illumina NextSeq instrument (Ilumina ...
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bioRxiv - Immunology 2021Quote: VH libraries from sorted B cells were subjected to NGS on the MiSeq platform with the reagent kit V3 2 × 300 bp paired-end (Illumina), using an input concentration of 16 pM with 5% PhiX ...
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bioRxiv - Microbiology 2020Quote: ... The marbled eel adomavirus RCA products and discus total DNA were prepared with a Nextera XT DNA Sample Prep kit and sequenced using the MiSeq (Illumina) sequencing system with 2 × 250 bp paired-end sequencing reagents ...
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bioRxiv - Immunology 2021Quote: ... Libraries were sequenced on a NovaSeq 6000 Illumina platform to obtain 50-bp paired end reads (TruSeq Rapid kit; Illumina).
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bioRxiv - Immunology 2020Quote: ... 18 libraries were prepared for dual index paired-end sequencing on a HiSeq 2500 using a high-output kit (Illumina) at an average sequencing depth of 38.6e6 reads per library with > 93% of bases exceeding a quality score of 30 ...
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bioRxiv - Microbiology 2020Quote: ... paired-end cDNA libraries were prepared from 10 μg of total RNA following poly-A purification using the TruSeq Stranded mRNA kit (Illumina) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... Libraries were mixed together and prepared at a final concentration of 12 pM and run on MiSeq Reagent Kit v3 (Illumina) according to the manufacturer’s specifications.
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bioRxiv - Molecular Biology 2021Quote: The amplicons were sequenced on an Illumina MiSeq platform in 2 × 251 bp paired-end mode using MiSeq Reagent Nano Kit v2 500 cycles (Illumina). The sequenced reads were mapped to the human reference genome hg19 using the methylation analysis tool Bismark v0.18.1 (Babraham Bioinformatics)52 ...
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bioRxiv - Molecular Biology 2020Quote: ... The RNA libraries were sequenced at pair-end on NextSeq 500 Sequencer with Illumina NextSeq 500/550 high output kit v2.5 (150 cycles) (Illumina, 20024907). Salmon was used to map and quantify the reads against the worm database WS268 ...
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bioRxiv - Molecular Biology 2020Quote: Total RNA was isolated for two replicates of wildtype and PUS1 knockout HepG2 cell as described above and stranded poly(A)+ selected mRNA-seq libraries were performed by the MIT BioMicroCenter using the TruSeq Stranded mRNA Library Prep Kit from Illumina according to the manufacturers’ protocol ...
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bioRxiv - Molecular Biology 2020Quote: ... The libraries were prepared with the TruSeq Stranded mRNA Library Prep Kit followed by rRNA removal with Ribo-Zero Gold (Illumina) and were sequenced on a HiSeq4000 sequencer (Illumina).
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bioRxiv - Immunology 2020Quote: ... The six enriched libraries from bulk expansion were combined into one library pool and sequenced on a NovaSeq sequencer with a NovaSeq 6000 S4 Reagent Kit (300 cycles (Illumina). All enriched V(D)J libraries were targeted for sequencing depth of at least 5,000 raw read pairs per cell ...
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bioRxiv - Immunology 2021Quote: ... Amplified cDNA was fragmented and appended with dual-indexed bar codes using the Nextera XT DNA Library Preparation kit (Illumina). Libraries were validated by capillary electrophoresis on an Agilent 4200 TapeStation ...
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bioRxiv - Immunology 2020Quote: ... High-quality RNA (approximately 500 ng; RIN 8) was used for nondirectional paired-end mRNA library preparation (TruSeq Sample Preparation Kit; Illumina).
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bioRxiv - Immunology 2020Quote: ... Samples were prepared using the Lexogen 3’ QuantSeq mRNA-seq Library Prep Kit FWD (Lexogen) and sequenced on the Illumina NovsSeq 6000 system (Illumina).
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bioRxiv - Immunology 2020Quote: ... First strand cDNA was subjected to limited PCR amplification followed by Tn5 transposon-based fragmentation using the Nextera XT DNA Library Preparation Kit (Illumina). Samples were then PCR amplified for 12 cycles using barcoded primers such that each sample carries a specific combination of eight base Illumina P5 and P7 barcodes for subsequent pooling and sequencing ...