Labshake search
Citations for Illumina :
7101 - 7150 of 9193 citations for Mouse CXCL2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... gDNA was either submitted for sequencing at the Microbial Genome Sequencing Center at the University of Pittsburgh or prepared for whole genome sequencing in house using the Nextera XT DNA Library Preparation Kit (Illumina). Libraries were sequenced using the NextSeq 550 or MiSeq platforms ...
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bioRxiv - Microbiology 2020Quote: ... One ng of the chromosomal DNA obtained was used in a library preparation using the Nextera XT library preparation kit (Illumina) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... Illumina libraries with an average insert size of 300 bps were constructed from purified genomic DNA using the Nextera XT kit (Illumina) and sequenced on Illumina's NovaSeq platform in a paired end 140 bp read configuration ...
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bioRxiv - Microbiology 2019Quote: ... we performed a second PCR step to add unique dual-index combinations to each sample using the Nextera XT index kit (Illumina). Second-step PCR amplifications were performed in a total volume of 25 μl using 2.5 μl of the PCR products ...
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bioRxiv - Immunology 2019Quote: RNA-seq libraries for CCR6+ ILC3 were prepared with the TruSeq Stranded Total RNA Library Prep Gold Kit (Illumina, 20020598). The sequencing was performed using Illumina NextSeq instrument (Ilumina ...
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bioRxiv - Immunology 2021Quote: VH libraries from sorted B cells were subjected to NGS on the MiSeq platform with the reagent kit V3 2 × 300 bp paired-end (Illumina), using an input concentration of 16 pM with 5% PhiX ...
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bioRxiv - Microbiology 2020Quote: ... The marbled eel adomavirus RCA products and discus total DNA were prepared with a Nextera XT DNA Sample Prep kit and sequenced using the MiSeq (Illumina) sequencing system with 2 × 250 bp paired-end sequencing reagents ...
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bioRxiv - Immunology 2021Quote: ... Libraries were sequenced on a NovaSeq 6000 Illumina platform to obtain 50-bp paired end reads (TruSeq Rapid kit; Illumina).
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bioRxiv - Microbiology 2021Quote: ... Libraries were prepared using 1 µg of RNA with TruSeq® stranded mRNA Preparation Kit (Illumina, San Diego, CA, USA) followed by sequencing with a Novaseq 6000 sequencer using 150 base read lengths in paired-end mode (Illumina ...
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bioRxiv - Immunology 2020Quote: ... 18 libraries were prepared for dual index paired-end sequencing on a HiSeq 2500 using a high-output kit (Illumina) at an average sequencing depth of 38.6e6 reads per library with > 93% of bases exceeding a quality score of 30 ...
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bioRxiv - Microbiology 2020Quote: ... paired-end cDNA libraries were prepared from 10 μg of total RNA following poly-A purification using the TruSeq Stranded mRNA kit (Illumina) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... Libraries were mixed together and prepared at a final concentration of 12 pM and run on MiSeq Reagent Kit v3 (Illumina) according to the manufacturer’s specifications.
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bioRxiv - Molecular Biology 2021Quote: The amplicons were sequenced on an Illumina MiSeq platform in 2 × 251 bp paired-end mode using MiSeq Reagent Nano Kit v2 500 cycles (Illumina). The sequenced reads were mapped to the human reference genome hg19 using the methylation analysis tool Bismark v0.18.1 (Babraham Bioinformatics)52 ...
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bioRxiv - Molecular Biology 2020Quote: ... The RNA libraries were sequenced at pair-end on NextSeq 500 Sequencer with Illumina NextSeq 500/550 high output kit v2.5 (150 cycles) (Illumina, 20024907). Salmon was used to map and quantify the reads against the worm database WS268 ...
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bioRxiv - Molecular Biology 2020Quote: Total RNA was isolated for two replicates of wildtype and PUS1 knockout HepG2 cell as described above and stranded poly(A)+ selected mRNA-seq libraries were performed by the MIT BioMicroCenter using the TruSeq Stranded mRNA Library Prep Kit from Illumina according to the manufacturers’ protocol ...
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bioRxiv - Molecular Biology 2020Quote: ... The libraries were prepared with the TruSeq Stranded mRNA Library Prep Kit followed by rRNA removal with Ribo-Zero Gold (Illumina) and were sequenced on a HiSeq4000 sequencer (Illumina).
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bioRxiv - Immunology 2020Quote: ... The six enriched libraries from bulk expansion were combined into one library pool and sequenced on a NovaSeq sequencer with a NovaSeq 6000 S4 Reagent Kit (300 cycles (Illumina). All enriched V(D)J libraries were targeted for sequencing depth of at least 5,000 raw read pairs per cell ...
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bioRxiv - Immunology 2021Quote: ... Amplified cDNA was fragmented and appended with dual-indexed bar codes using the Nextera XT DNA Library Preparation kit (Illumina). Libraries were validated by capillary electrophoresis on an Agilent 4200 TapeStation ...
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bioRxiv - Immunology 2020Quote: ... High-quality RNA (approximately 500 ng; RIN 8) was used for nondirectional paired-end mRNA library preparation (TruSeq Sample Preparation Kit; Illumina).
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bioRxiv - Immunology 2020Quote: ... Samples were prepared using the Lexogen 3’ QuantSeq mRNA-seq Library Prep Kit FWD (Lexogen) and sequenced on the Illumina NovsSeq 6000 system (Illumina).
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bioRxiv - Immunology 2020Quote: ... First strand cDNA was subjected to limited PCR amplification followed by Tn5 transposon-based fragmentation using the Nextera XT DNA Library Preparation Kit (Illumina). Samples were then PCR amplified for 12 cycles using barcoded primers such that each sample carries a specific combination of eight base Illumina P5 and P7 barcodes for subsequent pooling and sequencing ...
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bioRxiv - Immunology 2021Quote: ... paired-end libraries were prepared from total RNA by ribosomal depletion using the Yeast Ribo-Zero rRNA Removal Kit (Illumina) and then using the TruSeq Stranded total RNA-Seq kit (Illumina ...
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bioRxiv - Immunology 2020Quote: ... One ng of pre-amplified cDNA was used to generate barcoded Illumina sequencing libraries (Nextera XT library preparation kit - Illumina) in 8 μl reaction volume ...
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bioRxiv - Immunology 2020Quote: ... Pooled libraries were sequenced using the NextSeq 500/550 High Output Kit v2 for 75 cycles (Illumina FC-404-2005). Base calling accuracy was greater than 80% (>=Q30 ...
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bioRxiv - Immunology 2021Quote: ... PolyA RNA selected RNA Libraries were generated by the LMS Core Genomics Facility using the TruSeq Stranded mRNA Library Prep Kit (Illumina). Paired-ended sequencing was performed on an Illumina HiSeq 2500 sequencer ...
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bioRxiv - Immunology 2020Quote: ... and cDNA libraries were generated using Illumina Nextera XT kit (Illumina, Inc., 5200 Illumina Way San Diego, CA, 92122 USA). Next Generation Sequencing and quality control were performed at Medgenome Inc (Foster City ...
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bioRxiv - Immunology 2020Quote: ... the 2nd SGA env PCR products were cleaved into approximately 300 bp products by tagmentation using the Nextera DNA Library Prep Kit (Illumina). Indices from the Nextera Index Kit (Illumina) ...
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bioRxiv - Immunology 2021Quote: ... Libraries from 24 samples with unique identifiers were pooled and run on an Illumina NextSeq 500 using the 75 cycles NextSeq High Output kit (Illumina).
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bioRxiv - Immunology 2020Quote: ... 0.3 to 0.5 ng of pre-amplified cDNA was used to generate barcoded Illumina sequencing libraries (Nextera XT library preparation kit, Illumina) in an 8μL reaction volume ...
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bioRxiv - Microbiology 2021Quote: ... Libraries were prepared following the RNA-seq sample work flow using the Scriptseq Complete kit for bacteria and indexed with unique Scriptseq Index PCR primers (Illumina).
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bioRxiv - Immunology 2020Quote: ... Single cell libraries from sorted samples were generated using a UMI-based droplet-partitioning platform with the Chromium single cell 3’ library and gel bead kit v2 and v3 (10X Genomics) and then sequenced using a NovaSeq S2 (Illumina). Technical information relevant to the scRNA-Seq samples is found in Table S3.
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bioRxiv - Immunology 2021Quote: ... Stranded RNA libraries were then prepared from 1 μg total RNA using the TruSeq Stranded mRNA Prep Kit (Illumina, USA) following the manufacturer’s protocol ...
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bioRxiv - Immunology 2020Quote: ... Samples from the experiment comparing splenic NKTFH and NKTeff cells from αGalCer-challenged mice with unchallenged splenic iNKT cells were sequenced via a 100 x 100 bp paired-end read strategy using the NovaSeq6000 sequencing platform (NovaSeq 6000 S4 P200 kits - Illumina). Sequencing for all other samples was performed according to a 50 bp single-end strategy using the HiSeq2500 sequencer (HiSeq SBS Kit v4 ...
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bioRxiv - Immunology 2020Quote: ... Sequencing for all other samples was performed according to a 50 bp single-end strategy using the HiSeq2500 sequencer (HiSeq SBS Kit v4; Illumina). Post-sequencing ...
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bioRxiv - Microbiology 2021Quote: ... The library pool was then loaded into flow cell of MiSeq Reagent Kit V2 (2 × 150 cycles) and sequenced in a MiSeq platform (Illumina), according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: Libraries for each sample were sequenced on a NextSeq550 75 Cycle High Output sequencing kit (Illumina Inc., Sunnyvale, CA, USA). For each library ...
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bioRxiv - Immunology 2021Quote: ... ii) fragmentation and adapter ligation (NEBNext® UltraTM II FS DNA Library Prep Kit for Illumina, NEB, cat. no. E7805S), and iii ...
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bioRxiv - Microbiology 2021Quote: ... The clustering of the index-coded samples was performed on a cBot cluster generation system using HiSeq PE Cluster Kit v4-cBot-HS (Illumina) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2021Quote: ... using the TruSeq DNA PCR-free library prep kit with fragments of 150 bp and ii.) Nextera Mate Pair library prep with fragments of 2,000 bp (Illumina, USA). The short-read paired-end libraries were sequenced in two independent lanes and the mate-pair library in one lane using the Illumina HiSeq4000 platform (Illumina ...
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bioRxiv - Genomics 2021Quote: ... Canada) using the 300-cycle MiSeq Reagent Kit V2 on a MiSeq 2.0 Nano flowcell (Illumina, San Diego, CA, USA), paired-end 2×150 bp ...
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bioRxiv - Genomics 2021Quote: ... The index-coded samples were clustered with a cBot Cluster Generation System using a HiSeq 4000 PE Cluster Kit (Illumina) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2021Quote: ... Single and paired-end sequencing (150 to 250 cycles, V2 kits) was done on an Illumina MiSeq instrument with automatic adapter trimming (Illumina). Raw reads in fastq format that passed filters were used for analysis using CLC Genomics Workbench 12.0 (Qiagen) ...
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bioRxiv - Genomics 2021Quote: ... The libraries were sequenced on the S4 300 cycle kit flow (2×151 paired end reads) using the XP workflow as outlined by Illumina.
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bioRxiv - Evolutionary Biology 2021Quote: ... Sequencing libraries were prepared from 1µg DNA using the TruSeq PCRfree DNA sample preparation kit (cat# FC-121-3001/3002, Illumina Inc.) targeting an insert size of 350bp ...
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bioRxiv - Genomics 2021Quote: ... The 20 ul preamp was then mixed with 25 ul TD buffer and 5 ul TDE1 buffer (Nextera kit, FC-131-1096, Illumina) and incubated for 5 min at 55 C and held at 10 C ...
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bioRxiv - Genomics 2021Quote: ... 50 ng of amplicons were used for the library generation with the TruSeq Nano DNA Library Prep kit for NeoPrep (Illumina). The sequencing was conducted on a HiSeq1500 (Illumina ...
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Glycogen Synthase Kinase 3 regulates the genesis of the rare displaced ganglion cell retinal subtypebioRxiv - Neuroscience 2021Quote: ... Stranded RNA-Seq libraries were constructed from 100 ng high-quality total RNA (RIN > 8) using the TruSeq Stranded mRNA Library Preparation Kit (Illumina). Paired-end sequencing of 40 bases length was performed on a NextSeq 500 system (Illumina) ...
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bioRxiv - Genomics 2021Quote: ... Genomic DNA library was prepared and sequenced using a TruSeq DNA PCR-Free Library Prep Kit (Illumina, San Diego, CA).
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bioRxiv - Genomics 2021Quote: Plate-based RNA sample prep was performed on the PerkinElmer Sciclone NGS robotic liquid handling system using Illumina’s TruSeq Stranded mRNA HT sample prep kit utilizing poly-A selection of mRNA following the protocol outlined by Illumina in their user guide (https://support.illumina.com/sequencing/sequencing_kits/truseq-stranded-mrna.html ...
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bioRxiv - Genomics 2021Quote: ... and adapter ligation) of the biotinylated DNA was done manually and adapter ligation was done using a TruSeq DNA Index kit (Illumina). The Hi-C library was quantified using a KAPA library quantification kit (Roche) ...