Labshake search
Citations for Illumina :
651 - 700 of 793 citations for Human Immunodeficiency Virus GP41 Protein HIV 1 Group O since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Genomics 2024Quote: ... The sequencing library was prepared with 1 μg of DNA using the TruSeq PCR-free DNA sample preparation kit (cat# 20015962, Illumina), according to the TruSeq DNA PCR-free Reference Guide ...
-
bioRxiv - Immunology 2024Quote: ... the antibody barcode library was pooled at a ratio of 1:5 with the gene expression library and sequenced on a NextSeq 2000 (Illumina) to an average depth of 34,000 reads per cell.
-
bioRxiv - Neuroscience 2024Quote: ... Libraries were sequenced Paired-End 51 bases (in addition: 8 bases for index 1 and 8 bases for index 2) setup using the NovaSeq 6000 instrument (Illumina). SP Flow-Cell was loaded at a final concentration in Flow-Lane loaded of 400pM and including 1% PhiX ...
-
bioRxiv - Neuroscience 2024Quote: ... Libraries were sequenced Single-reads 76 bases (in addition: 8 bases for index 1 and 8 bases for index 2) on NextSeq 500 (Illumina) using the NextSeq 500 High Output Kit 75-cycles (Illumina ...
-
bioRxiv - Neuroscience 2023Quote: ... Female samples after MR2 were sequenced (single-end reads, 1×50 nt) with Illumina NextSeq 2000 (Illumina, San Diego, USA). Quality checks and sequencing were performed by StarSEQ GmbH (Mainz ...
-
bioRxiv - Genetics 2024Quote: ... we aligned the corresponding short reads to the human reference GRCh38 using bwa mem23 (v 0.7.17-r1188; -t 48 -R “@RG\tID:1\tLB:lib1\tPL:ILLUMINA\tSM:
\tPU:unit1”). Next ... -
bioRxiv - Molecular Biology 2024Quote: ... CUT&RUN libraries (10–20 Mio reads per sample and 1/3 of reads for the controls) were sequenced on a NovaSeq 6000 (Illumina) paired end 100 bp at the Deep Sequencing Facility (Max Planck Institute for Immunobiology and Epigenetics ...
-
bioRxiv - Molecular Biology 2024Quote: ... Illumina indexes were added by amplifying 1 ng DNA to a 50 μl PCR reaction using Kapa HS enzyme and unique dual indexing primers (Illumina) for 11 cycles (Denaturation 95°C 3min ...
-
bioRxiv - Cancer Biology 2024Quote: ... the sequencing was performed under the setting of single read 1×51 bp to generate ∼30 million reads per sample on HiSeq 1000 sequencer (Illumina).
-
bioRxiv - Cancer Biology 2024Quote: ... Libraries were pooled and single-end sequenced (1×75 bp) on the Illumina NextSeq 500 using the High-Output V2 Kit (Illumina). Read data were processed in BaseSpace ...
-
bioRxiv - Cancer Biology 2023Quote: ... Libraries were pooled and single end sequenced (1×75) on the Illumina NextSeq 500 using the High output V2 kit (Illumina).
-
bioRxiv - Systems Biology 2024Quote: ... according to the Ovation RRBS Methyl-Seq System 1-16 protocol for the first read and the Read 2 primer (Illumina) for the second read ...
-
bioRxiv - Microbiology 2020Quote: ... MDA-amplified PCR products were then diluted to DNA concentrations of 0.2 ng μL−1 and used in metagenomic library preparations with the Nextera XT DNA Library Prep Kit (Illumina, San Diego), then quantified on an Agilent 2100 Bioanalyzer System (Agilent Genomics ...
-
bioRxiv - Molecular Biology 2020Quote: Libraries of eDNA and iDNA samples at 50 ng μL−1 were sequenced using a MiSeq PE300 benchtop sequencer (Illumina, USA). Library preparation was done with a TruSeq DNA PCR-Free kit (LT Lib PREP KT-ST B PhiX control v3) ...
-
bioRxiv - Developmental Biology 2021Quote: ... Illumina TruSeq RNA libraries were prepared from 1 μg total RNA and sequenced to generate 50 base pair single-end reads using the the HiSeq 1500 (Illumina, USA) at the Children’s Mercy OMICs Research Core Lab (Kansas City ...
-
bioRxiv - Microbiology 2019Quote: ... Illumina recommended denaturation and loading recommendations which included a 1% PhiX spike in (PhiX Control v3 Illumina Catalogue FC-110-3001). Whole genome sequencing data has been deposited in the Sequence Read Archive under PRJNA529870.
-
bioRxiv - Bioengineering 2019Quote: ... sequencing adaptors and unique dual barcodes combinations were attached to each library PCR amplicons using the Nextera XT Index Kit primers (index 1 N7XX and index 2 S5XX, Illumina, CA), and the amplicon size and concentration were confirmed using a Bioanalyzer (Agilent ...
-
bioRxiv - Neuroscience 2020Quote: ... RNA-seq libraries were prepared from 1 μg of total RNA using the TruSeq Stranded mRNA LT Sample Prep kit (Illumina, USA). Sequencing was performed on the Illumina NovaSeq 6000 (CNS-RNA-seq cohort subset ...
-
Cis-regulatory divergence underpins the evolution of C3-C4 intermediate photosynthesis in MoricandiabioRxiv - Plant Biology 2021Quote: ... cDNA libraries were prepared using 1 μg of total RNA with the TruSeq RNA Sample Preparation Kit (Illumina, San Diego, USA). The cDNA library was qualified on the Agilent Technologies 2100 Bioanalyzer to check the library quality and fragment size of the sample ...
-
bioRxiv - Cell Biology 2021Quote: ... Sequencing libraries were constructed from 1 ng of pre-amplified cDNA using DNA library preparation kit (TruePrep DNA Library Prep Kit V2 for Illumina, Vazyme). Libraries were sequenced on a HiSeq-PE150 ...
-
bioRxiv - Cancer Biology 2020Quote: ... 1 µg of total RNA was used for library preparation using the TruSeq Stranded mRNA kit (Illumina, cat# RS-122-2103) after rRNA depletion using Ribo-Zero plus (Illumina ...
-
bioRxiv - Microbiology 2022Quote: ... 1 µg of gDNA per sample was analyzed using the TruSeq® DNA PCR-Free Low-Throughput Library Prep Kit (Illumina) according to the manufacturer’s directions ...
-
bioRxiv - Microbiology 2022Quote: ... and sequenced on an Illumina NovaSeq using single-end 1×100 reads with the Illumina NovaSeq SP reagent kit (Illumina, 20027464). A total of 122,190,150 raw sequence reads (mean of 4,213,453 per sample (n = 29 ...
-
bioRxiv - Cell Biology 2019Quote: ... Total RNA (1 μg) was used for extracting rRNA using the Ribo-Zero rRNA Removal Kit (Illumina, San Diego, CA, USA) according to the manufacturer’s instructions ...
-
bioRxiv - Genomics 2019Quote: ... The sequencing was performed by doing a cluster generation and 150 cycles paired-end sequencing of the 24 libraries in 1 lane using the HiSeq X system and v2.5 sequencing chemistry (Illumina Inc., USA). Read data were delivered to us as fastq.
-
bioRxiv - Molecular Biology 2021Quote: RNA-seq libraries were prepared with 1 µg of total RNA for each sample using the Illumina TruSeq mRNA kit (Illumina, USA) as recommended by the manufacturer using the Illumina CD RNA indexes (Illumina ...
-
bioRxiv - Systems Biology 2020Quote: ... Final loading concentration of 1.8 pM and 1% PhiX (v/v) was used with the NextSeq® 500/550 High Output Kit v2 (150 cycles) (Illumina). Samples were sequenced paired-end with 75 cycles and single index of 6 cycles.
-
bioRxiv - Immunology 2021Quote: ... Samples were sequenced on an Illumina Novaseq 6000 S4 flowcell (67 bp read 1 and 140 bp read 2) (Illumina, USA) to a depth of approximately 100,000 reads per cell ...
-
bioRxiv - Immunology 2020Quote: ... Pooled libraries of 1 nM were denatured and diluted as per the Standard Normalization Method for the NextSeq® System (Illumina). Final loading concentration of 1.7 pM and 1% (v/v ...
-
bioRxiv - Immunology 2020Quote: ... Final loading concentration of 1.7 pM and 1% (v/v) PhiX was used with the NextSeq® 500/550 High Output Kit v2 (150 cycles) (Illumina Samples were sequenced paired-end with 75 cycles of each read and single index of 6 cycles.
-
bioRxiv - Immunology 2021Quote: ... whose lengths were about 600 base pairs were sequenced using an Illumina Novaseq 6000 S4 flowcell (67 bp read 1 and 140 bp read 2) (Illumina, USA). Only read2 contained the sequence regarding the definition of T cell clones.
-
bioRxiv - Molecular Biology 2022Quote: ... PolyA + fraction (1 ng for each sample) was used for whole-transcriptome library preparation using ScriptSeq v2 RNA-Seq kit (Illumina, USA). Libraries amplified in 14 PCR cycles were purified using Agencourt AMPure XP beads (Beckman-Coulter ...
-
bioRxiv - Neuroscience 2022Quote: ... Single-end sequencing (i.e, 1×75bp) was performed in a single run on an Illumina NextSeq 500 (Illumina, San Diego, CA). Three independent experimental replicates were run for the Cebpg shRNA-mediated knockdown and shRNA control transgenic lines for a total of 6 libraries.
-
bioRxiv - Cancer Biology 2023Quote: ... pre-treatment and post-treatment/acquired resistant biopsies were obtained from patients receiving various immune checkpoint inhibitor treatments (PD-L1, PD-1, CTLA-4 targeted therapies) for RNA-seq analysis (Illumina HiSeq2500) from formalin fix paraffin embedded samples ...
-
bioRxiv - Cancer Biology 2023Quote: ... RNAseq libraries were prepared from total RNA with the QuantSeq 3′ mRNA-Seq Library Prep Kit FWD for Illumina (Lexogen, 015.24) in combination with the UMI Second Strand Synthesis Module for QuantSeq FWD (Illumina, Read 1) (Lexogen ...
-
bioRxiv - Genetics 2023Quote: ... was used to isolate total RNA and approximately 1 ug of RNA was processed with the TruSeq Stranded mRNA Library Prep Kit (Illumina 20020594) to prepare libraries for paired-end sequencing on Nova-seq 6000 ...
-
bioRxiv - Developmental Biology 2023Quote: ... PolyA+ RNA-seq libraries were produced from 1 µg of RNA using the TruSeq® Stranded mRNA Library Prep (Illumina, 20020594) kit ...
-
Dysregulated expanded endocannabinoid system as therapeutic targets of amyotrophic lateral sclerosisbioRxiv - Neuroscience 2024Quote: ... A library was independently prepared with 1 µg of total RNA for each sample by an Illumina TruSeq Stranded mRNA Sample Prep Kit (Illumina, Inc.). The first step in the workflow involved purifying the poly-A-containing mRNA molecules using poly-T-attached magnetic beads ...
-
bioRxiv - Plant Biology 2024Quote: ... total RNA from Col-0 and hakai-1 (Mock and Hpa) three biological replicates (same samples used for Illumina RNA-seq) were pooled into a single sample ...
-
bioRxiv - Microbiology 2024Quote: Libraries for Illumina short read sequencing were prepared from 1 ng of extracted DNA utilizing the Nextera XT DNA Library Prep Kit according to the manufacturer’s recommendations (Illumina Inc., USA). Sequencing was carried out in paired-end (2×300 base pairs ...
-
bioRxiv - Genomics 2021Quote: ... Total RNA (1 μg) from each sample was used for NEBNext PolyA selection and Ultra Directional RNA Library preparation (Illumina, E7490, E7420). Prepared libraries were sequenced using 2×150 bp paired-end read chemistry on an Illumina HiSeq 4000 platform.
-
bioRxiv - Molecular Biology 2021Quote: ... 1 ug of treated RNA was then input into the Illumina TruSeq Small RNA Library Preparation Kit (Illumina; Cat #: RS-200-0012) with 11 cycles of PCR amplification ...
-
bioRxiv - Genomics 2020Quote: ... Samples were prepared for sequencing using 1 ug of genomic DNA following the TruSeq PCR free kit protocol (Illumina, FC-121-3001). Resulting libraries were quality checked on an Agilent DNA 1000 bioanalyzer (Agilent Technologies ...
-
bioRxiv - Genomics 2019Quote: ... 500bp and 800bp) and long insert mate-pair reads (4-6Kb, 8-10Kb and 1-20Kb) were sequenced using HiSeq 2500 (Illumina Inc., USA) at SciGenom Labs ...
-
bioRxiv - Genomics 2019Quote: TruSeq libraries were prepared with 1 µg of total RNA using the TruSeq Stranded mRNA Library Prep Kit (Illumina, #RS-122-2101) and following the manufacturer's instructions ...
-
bioRxiv - Plant Biology 2020Quote: 42 RNA samples from experiment 1 were used for mRNA-Seq library preparation using the TruSeq Stranded mRNA Library Prep Kit (Illumina, CA, USA). Libraries were multiplexed and single-end 100 nt RNA-Seq was performed on a Novaseq 6000 at the GIGA Center (University of Liege ...
-
bioRxiv - Genomics 2022Quote: ... a library was prepared from 1 μg of genomic DNA and a TruSeq DNA PCR-free Sample Preparation Kit (Illumina, CA, USA). Paired-end (151 bp per read ...
-
bioRxiv - Genomics 2022Quote: ... Libraries were prepared with 1 μg of total RNA for each sample using a TruSeq Stranded mRNA Sample Prep Kit (Illumina, CA, USA). Paired-end sequencing (151 bp ...
-
bioRxiv - Microbiology 2022Quote: ... The V4 region of the 16S rRNA gene was amplified from 1 or 7μl DNA and sequenced with a MiSeq (Illumina, San Diego, CA) using the 500 cycle MiSeq Reagent Kit ...
-
bioRxiv - Cell Biology 2020Quote: ... cDNA was diluted 1:15 and subsequently tagmented and amplified for 12 cycles using the Nextera XT DNA Library Preparation Kit (Illumina FC-131). Individual libraries were QC’d by Qubit and Agilent Bioanalyzer ...