Labshake search
Citations for Illumina :
6751 - 6800 of 9151 citations for Rat Endoplasmin HSP90b1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: ... MEFs and NPCs were used to prepare NGS libraries based on the NEB Next Ultra II directional RNA library preparation kit from Illumina. As default ...
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bioRxiv - Microbiology 2022Quote: ... After DNAse treatment 50ng-1ug of total RNA was used to prepare DNA libraries with Truseq RNA Library Prep Kit v2 (Illumina) following manufacture’s instruction ...
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bioRxiv - Microbiology 2022Quote: ... 1 ug of total RNA from each tissue was enriched for polyadenylated RNA and prepared for next-generation sequencing using the TruSeq Stranded mRNA Library Prep Kit (Illumina) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... The viral spike sequences were verified using unbiased NGS sequencing (KAPA HyperPrep library kit with RiboErase, followed by Illumina sequencing). All cells and virus stocks tested negative for mycoplasma using MycoAlert Plus reagents (Lonza) ...
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bioRxiv - Genomics 2022Quote: ... A library was prepared using a TruSeq DNA PCR-Free library preparation kit according to manufacturer guidance and was sequenced on a NextSeq500 instrument (Illumina) with a NextSeq High Output v2 300 cycle kit ...
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bioRxiv - Genomics 2022Quote: Whole-Genome sequencing libraries were prepared from 250 ng of genomic DNA by tagmentation with the NexteraFlex kit from Illumina. Subsequently ...
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bioRxiv - Microbiology 2022Quote: ... Illumina sequencing libraries were prepared using the Nextera XT sample prep kit and sequenced on the Illumina MiSeq platform (Illumina) with 300-bp paired-end read configuration (MiSeq Reagent Kit v3) ...
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bioRxiv - Microbiology 2022Quote: ... Messenger RNA was enriched by first depleting ribosomal RNA from samples using the Ribo-Zero rRNA removal (bacteria) magnetic kit (Illumina). The purified mRNA was prepared for Illumina sequencing using the NEBNext Ultra RNA Library Prep Kit for Illumina according to the manufacturer’s instructions in combination with AMPure XP reagent for clean-up of adaptor-ligated DNA ...
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bioRxiv - Microbiology 2022Quote: ... RNAseq libraries were prepared from total RNA using the Nextera DNA Flex library preparation kit (Illumina, Inc., San Diego, CA) according to the manufacturer’s instructions where the first-strand cDNA was synthesized using SuperScript II Reverse Transcriptase (Thermo Fisher ...
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bioRxiv - Genomics 2022Quote: ... Illumina library prep was performed using Nextera DNA Flex Library Preparation kit (cat no. 20018704) and sequenced on the iSeq100 (Illumina).
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bioRxiv - Neuroscience 2022Quote: ... and finally converted into DNA libraries using custom rpi primers (RNA PCR Primer Index) adapted from the Truseq Small RNA kit (Illumina)106 ...
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bioRxiv - Genomics 2022Quote: ... A paired-end library was prepared with the TruSeq DNA PCR-Free LT Sample Preparation Kit (Illumina, #FC-121-3001) from 1 µg of the genomic DNA ...
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bioRxiv - Microbiology 2022Quote: ... Further library preparation of rRNA-depleted samples was performed using TruSeq Stranded mRNA library preparation kit (Illumina, RS-122-2101) according to the manufacturer’s instructions apart from omitting the initial steps for mRNA pull-down ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Sequencing was performed on a MiSeq system with the reagent kit v3 with 600 cycles (Illumina, San Diego, CA, USA) as recommended by the manufacturer and resulted in a minimum average coverage of 88× per strain (coverage range was from 88× to 157×) ...
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bioRxiv - Cell Biology 2022Quote: ... RNA library was constructed with 10x-Genomics Single Cell 3’ RNA sequencing kit v3 as previously described(18) sequenced using a NextSeq500 (Illumina). Cell clusters were characterized using an automated annotation tool(19 ...
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bioRxiv - Immunology 2022Quote: ... The clustering of the index-coded samples was performed on a cBot Cluster Generation System using PE Cluster Kit cBot-HS (Illumina) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2022Quote: ... Illumina sequencing libraries were prepared from the PCR-amplified cDNA from mutant lungs and controls using the Nextra DNA library prep kit (Illumina). Libraries were sequenced on a NovaSeq 6000 S Prime flowcell (Illumina ...
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bioRxiv - Cell Biology 2022Quote: The iROND samples and embryonic RNA samples were reverse transcribed into cDNA libraries using the TruSeqTM RNA Sample Preparation Kit (Illumina). RNA sequencing was performed with an Illumina HiSeq™ 3000 or HiSeq X Ten platform ...
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bioRxiv - Cell Biology 2022Quote: ... The clustering of the index-coded samples was performed on a cBot Cluster Generation System using PE Cluster Kit cBot- HS (Illumina) according to the manufacturer’s instructions.
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bioRxiv - Neuroscience 2021Quote: ... ~600 pg of purified cDNA was used to produce a RNA-seq library using Nextera XT DNA Library Preparation kit (FC-131-1024, Illumina), following the manufacturer instructions.
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bioRxiv - Microbiology 2020Quote: ... products above the typical 75-nucleotide phage attP were gel purified and prepped for deep-sequencing with the TruSeq Nano kit (Illumina). DNA was sequenced using 150-nucleotide single-end read with an Illumina MiSeq ...
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bioRxiv - Molecular Biology 2020Quote: One μg of total RNA of high quality (RIN>8) was used for sequencing with a TruSeq RNA sample preparation kit (Illumina). We here included 99 islet samples in addition to the 89 islet samples and processed them uniformly following the same protocol as described previously (8) ...
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bioRxiv - Microbiology 2020Quote: Clustering of index-coded samples was performed on a cBot Cluster Generation System using TruSeq PE Cluster Kit v4-cBot-HS (Illumina) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2021Quote: Sequencing libraries were prepared with the Illumina TruSeq stranded mRNA sample preparation kit according to the manufacturer’s protocol (Illumina, USA). In brief ...
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bioRxiv - Plant Biology 2021Quote: ... This modified version of the SMART-seq2 and SCRB-seq protocols allowed multiplexed pooling of 96 samples before library preparation with the Nextera XT DNA sample prep kit (Illumina). The protocol returned 3’-biased cDNA fragments similar to the Drop-Seq protocol (Macosko et al. ...
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bioRxiv - Microbiology 2020Quote: ... The clustering of the index-coded samples was performed on a cBot Cluster Generation System using TruSeq PE Cluster Kit v3-cBot-HS (Illumina), and the library preparations were then sequenced on an Illumina NovaSeq 6000 platform and 150 bp paired-end reads were generated ...
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bioRxiv - Immunology 2020Quote: ... Use of 250 pg of cDNA with 1/5 reaction of Illumina Nextera XT kit (Illumina, San Diego, CA, USA). The length distribution of the cDNA libraries was monitored using DNA High Sensitivity Reagent Kit on the Perkin Elmer Labchip GX system (PerkinElmer ...
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bioRxiv - Immunology 2020Quote: ... PCR products for TCRα and TCRβ were either subjected to Sanger sequencing or multiplexed with barcodes and subjected to MiSeq sequencing (Han et al., 2014) using True Seq Nano kit (Illumina). For Miseq data ...
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bioRxiv - Immunology 2020Quote: ... The indexed libraries were pooled and diluted to 1.5pM for paired end sequencing (2× 76 cycles) on a NextSeq 500 instrument using the v2 150 cycle High Output kit (Illumina) as per manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... Biological triplicate samples for each strain were then pooled at equal RNA concentrations and ribosomal RNA was removed using a Ribo-Zero Kit for Gram Positive Bacteria (Illumina). Following this ...
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bioRxiv - Microbiology 2021Quote: ... The purified DNA was then diluted to 0.25 ng/μL and prepared for sequencing with the Nextera XT DNA library prep kit (Illumina, USA). Libraries were sequenced on an Illumina MiSeq using a 300 cycle v2 kit (150 nt paired end reads) ...
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bioRxiv - Microbiology 2021Quote: ... Library preparation and sequencing was carried out by MR DNA following Illumina library preparation protocols using MiSeq Reagent Kit v3 (Illumina).
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bioRxiv - Developmental Biology 2021Quote: ... Libraries were generated using Kapa Biosystems library preparation kit (#KK8201) and multiplexed libraries were sequenced on a 1×75 flow cell on the HiSeq2000 platform (Illumina). Reads were filtered and trimmed to remove adapter-derived or low-quality bases using Trimmomatic and checked again with FASTQC ...
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bioRxiv - Developmental Biology 2021Quote: ... sRNA libraries for dicer-null embryos were prepared using Illumina TruSeq Small RNA Sample Prep kit (Illumina, RS-200-0012) and sequenced on HiSeq 50bp single-end sequencing platform ...
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bioRxiv - Neuroscience 2020Quote: ... Five hundred ng of RNA per sample was used to prepare a dual-indexed strand-specific cDNA library using TruSeq Stranded mRNA library Prep Kit (Illumina). The resulting libraries were assessed for their quality and size distribution using Qubind Agilent 2100 Bioanalyzer ...
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bioRxiv - Cancer Biology 2020Quote: ... then 60 ng was used for library preparation using KAPA Stranded RNA-Seq Library Preparation Kit (Illumina, KR0934 – v 1.13). The samples were barcoded and pooled in sets of eight per run to generate paired 150-bp reads with NextSeq 550 Sequencing System (Illumina) ...
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bioRxiv - Microbiology 2020Quote: ... Libraries diluted to 4 nM were sequenced with NextSeq 500 High Output Kit v2 (75 cycles) on a NextSeq 500 (Illumina). Small RNA libraries have been submitted to the NCBI sequence read archive (SRA ...
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bioRxiv - Plant Biology 2021Quote: ... Libraries were constructed using the Nextera DNA preparation kit and sequenced on an Illumina Hi-Seq 2500 platform (paired-end 125 bp reads) (Illumina). Quality control reports for the raw sequencing reads were generated using FastQC (Andrews ...
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bioRxiv - Plant Biology 2021Quote: ... Samples with a RIN value of > 7 were used for library preparation (Illumina TruSeq Stranded Total RNA kit, Illumina, USA) and sequenced on an Illumina HiSeq 4000 to obtain 150bp paired-end reads ...
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bioRxiv - Microbiology 2020Quote: Sequencing libraries were prepared for each product of long PCR (ranging from 15 to 33 kb) using the Nextera XT DNA Sample Preparation Kit (Illumina) and sequenced on the Illumina MiSeq platform to generate paired-end (PE ...
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bioRxiv - Microbiology 2021Quote: ... Sequencing was performed by employing an Illumina HiSeq 2500 system and the HiSeq Rapid SBS kit V2 (2×250 bp) as recommended by the manufacturer (Illumina). Metagenomic reads were further processed as described previously (Eze et al ...
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bioRxiv - Genomics 2020Quote: ... Wild-type cultured aortic ECs were used to generate the immunoprecipitated and input control libraries using TruSeq DNA Sample Prep Kit v2 (Illumina) following manufacturer’s direction ...
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bioRxiv - Microbiology 2021Quote: ... 5–10 × 106 50-bp paired-end reads were obtained for each sample on an Illumina HiSeq 2500 using the TruSeq SBS Kit v3 (Illumina). Post-run demultiplexing and adapter removal were performed and fastq files were inspected using fastqc (Andrews S ...
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bioRxiv - Genomics 2019Quote: ... Library preparation was performed as per the 10X Genomics Genome Library protocol (https://support.10xgenomics.com/genome-exome/library-prep/doc/user-guide-chromium-genome-reagent-kit-v2-chemistry) and uniquely barcoded libraries were prepared and multiplexed for sequencing by Illumina HiSeq ...
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bioRxiv - Genomics 2019Quote: ... and then subjected to library construction using a TruSeq DNA PCR-Free HT sample prep kit (Illumina; San Diego, CA). The libraries were sequenced on a HiSeq 2500 system (Illumina ...
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bioRxiv - Cancer Biology 2021Quote: ... Tumor DNA was isolated using the Qiagen Blood and Tissue Kit and sgRNA cassette was amplified similarly to the in vitro screen and analyzed by Illumina sequencing ...
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bioRxiv - Microbiology 2021Quote: ... libraries were prepared as described previously (Steglich et al., 2018) and sequenced on an Illumina NextSeq 500 machine using a Mid-Output kit (Illumina) with 300 cycles ...
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bioRxiv - Microbiology 2021Quote: ... On-board cluster generation and paired-end sequencing was completed on the NextSeq 550 (Ilumina) using a High Output 150 cycle kit (Illumina). The average cluster density was 177 k/mm2 resulting in 420 million reads passing filter per run ...
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bioRxiv - Molecular Biology 2019Quote: Genomic DNA for whole-genome sequencing (WGS) analysis was isolated directly from bacterial colonies using the Nextera DNA Flex Microbial Colony Extraction kit (Illumina) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2020Quote: ... Each library was adjusted to a concentration of 20 pM and was sequenced with a MiSeq Reagent kit v3 for 150 cycles by an Illumina MiSeq Sequencing System (Illumina). An efficiency of 25 million sequence reads per flow cell was achieved.