Labshake search
Citations for Illumina :
6751 - 6800 of 9198 citations for Pyruvate Dehydrogenase Microplate Assay Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... In the second method purified PCR amplicons (100 ng) were used to prepare sequencing libraries by TruSeq Nano DNA LT Library Preparation Kit (Illumina) with the following modifications ...
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bioRxiv - Genomics 2020Quote: ... PCR amplified cDNA for each cell was examined using fragment analyzer (Advanced Analytical) and the concentration was normalized before it was used for library preparation (Nextera XT kit, Illumina). Primers were removed using AMpure XP beads (Beckman Coulter ...
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bioRxiv - Cell Biology 2021Quote: ... The generated cDNA was 3′ end-adenylated and ligated to Illumina Paired-end sequencing adapters and amplified by PCR using HiSeq SR Cluster Kit v4 cBot (Illumina). Libraries were analyzed on a 2100 Bioanalyzer (Agilent ...
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bioRxiv - Cancer Biology 2020Quote: The RNA-seq libraries (n=3 per experimental group) were prepared using the NEBNext Ultra II RNA library prep kit from Illumina (New England Biolabs Inc. ...
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bioRxiv - Cancer Biology 2020Quote: ... and then ribosomal RNA was removed from the samples by the Ribo-Zero kit (Illumina, San Diego, CA, United States). The sequencing library was prepared following the standard protocol for TruSeq Stranded Total RNA Sample Prep Kit (Illumina) ...
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bioRxiv - Cancer Biology 2020Quote: Strand specific RNA sequencing libraries were constructed using the Illumina TruSeq RNA Access Library Prep Kit (Illumina, San Diego, CA) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2020Quote: ... The clustering of the index-coded samples was performed on a cBot Cluster Generation System using PE Cluster Kit cBot-HS (Illumina) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... The clustering of the index-coded samples was performed on a cBot Cluster Generation System using HiSeq PE Cluster Kit cBot-HS (Illumina) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... and further used for the cDNA synthesis by using ScriptSeq™ v2 RNA-Seq kit (Epicentre, Illumina, Madison, WI USA). The cDNA was purified using AMPure XP (Beckman Coulter ...
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bioRxiv - Cancer Biology 2021Quote: ... the library was diluted at 4nM and loaded on the cartridge (MiSeq reagent kit V2 Nano 500 cycles; #MS-103-1003, Illumina) at 6 pM with 15% PhiX control DNA v3 (#FC-110-3001 ...
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bioRxiv - Cancer Biology 2021Quote: ... Edits were confirmed by sequencing after amplification using index primers based on the NexteraXT index kit V2 setA (#15052163, Illumina) and the high fidelity Taq polymerase Kapa (#KK2602 ...
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bioRxiv - Cell Biology 2021Quote: RNA isolated from colonic epithelial cells was prepared for next-generation sequencing using the stranded RNA library preparation kit with rRNA depletion (Illumina) and sequenced using an Illumina HiSeq 2500 with 100bp single reads ...
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bioRxiv - Cell Biology 2020Quote: ... 50,000 nuclei were purified from WT or Sun dKO cells and DNA was tagmented using the Nextera Library Prep Kit (Illumina). Tagmented DNA was purified using the Qiagen MinElute PCR Purification kit ...
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bioRxiv - Cell Biology 2021Quote: ... The preparation of RNAseq libraries were performed using the TruSeq Stranded mRNA Sample Prep Kit (Illumina, San Diego, CA, USA) at Novogene Co. ...
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bioRxiv - Cell Biology 2020Quote: ... Approximately 2 μg of total RNA was used to subselect poly(A)+ transcripts and generate strand-specific cDNA libraries (TrueSeq kit; Illumina).
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bioRxiv - Cancer Biology 2021Quote: ... Illumina-stranded total RNA libraries were prepared using the TruSeq Stranded Total RNA Sample Preparation Kit (Illumina, San Diego, CA). Briefly ...
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bioRxiv - Genomics 2020Quote: ... mouse cell lines and tumours for the purpose of retrotransposon and cryptic transcript expression analysis was performed using the KAPA Stranded mRNA-Seq Kit according to the provided protocol (Illumina). For expression analysis of coding genes ...
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bioRxiv - Cell Biology 2021Quote: Individually barcoded stranded mRNA-Seq libraries were prepared from high-quality total RNA samples (~600 ng/sample) using the Illumina TruSeq RNA Sample Preparation v2 Kit (Illumina) implemented on the liquid handling robot Beckman FXP2 ...
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bioRxiv - Genomics 2022Quote: Library preparation from 1-10ng rRNA-depleted RNA was performed using NEBNext UltraII Directional RNA Library Preparation Kit (Illumina, #E7760) following the vendor protocols and complementing cleaning steps with NEBNext Sample Purification Beads (#E7767) ...
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bioRxiv - Neuroscience 2022Quote: ... approximately 10 ng of total RNA per sample was used for library construction with the Ultra-Low-RNA-seq RNA Sample Prep Kit (Illumina) and sequenced using the Illumina HiSeq 4000 instrument according to the manufacturer’s instructions for 100bp paired end read runs ...
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bioRxiv - Synthetic Biology 2022Quote: ... sequencing was performed on an Illumina MiSeq (MiSeq Reagent kit v3, 300 cycles) following the manufacture’s run protocols (Illumina, Inc.). The MiSeq Control Software Version 2.2 ...
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bioRxiv - Molecular Biology 2022Quote: ... Both 5’-tir-P enriched and oxidized samples were subjected to a sRNA library construction by the TruSeq Small RNA Library Preparation kit (Illumina). The resulting libraries were sent to BGI (Hongkong ...
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bioRxiv - Molecular Biology 2022Quote: ... followed by library construction using non-stranded (Replicate 1) or stranded (Replicates 2 and 3) TruSeq mRNA Library Prep Kit (Illumina). The resulting libraries were sequenced on Hiseq4000.
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bioRxiv - Microbiology 2022Quote: ... Sequence libraries were prepared from the extracted DNA samples using the Nextera DNA Flex Library Prep Kit (Illumina, Cat. # 20018705) following a low volume variation of the standard protocol.29
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bioRxiv - Cell Biology 2022Quote: ... The resulting cDNAs from individual cells were used for the construction of NGS libraries with the Nextera XT DNA sample preparation kit (Illumina). Libraries were pooled ...
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bioRxiv - Biochemistry 2022Quote: ... 50 ng of DNase I treated total RNA and three-stage purified mRNA from the two biological replicates were fragmented using the TruSeq RNA Library Prep Kit v2 fragmentation buffer (Illumina). First-strand cDNA synthesis was performed using the random hexamer primer ...
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bioRxiv - Genetics 2022Quote: ... RNAs were sent to the genomic platform GeT-Plage (Toulouse, France) for the cDNA library preparation (TruSeq Stranded mRNA kit, Illumina) and the sequencing (NovaSeq 6000 Sequencing System ...
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bioRxiv - Neuroscience 2022Quote: Total RNAseq libraries were prepared from 1 microgram of total RNA from frozen brain tissue using the TruSeq Stranded Total RNA with Ribo-Zero Gold kit (Illumina) according to the protocol specifications ...
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bioRxiv - Developmental Biology 2022Quote: ... poly(A)-selected RNA-seq libraries were prepared by Novogene Co. using the NEBNext UltraII RNA library prep kit for Illumina (cat. #E7775) and sequenced on a NovaSeq6000 (Illumina) to produce paired-end 150-bp reads (average 40.4 million reads per sample) ...
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bioRxiv - Plant Biology 2022Quote: ... polymorpha wild-type and Mpgun1 mutant spores were prepared using 200 ng of DNase-treated total RNA as a template for the TruSeq Stranded Total RNA kit with Ribo-Zero Plant (Illumina). The libraries were sequenced on an Illumina HiSeq 4000 platform (150 nt paired-end reads ...
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bioRxiv - Immunology 2022Quote: ... 500 ng total RNA was used as starting material from which ribosomal RNA was depleted using Ribo-Zero Plus rRNA Depletion Kit (Illumina). The rRNA depleted samples were fragmented ...
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bioRxiv - Immunology 2022Quote: ... 1000ng RNA from each sample was used as a template for preparing Illumina compatible libraries using the TruSeq RNA Library Prep Kit v2 (Illumina). Library sizes were checked using D5000 high sensitivity tape on the TapesSation 2200 (Agilent) ...
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bioRxiv - Physiology 2022Quote: ... The pooled libraries were sequenced on NextSeq 500 Illumina sequencer using NextSeq 500/550 High Output Kit v2.5 (75 cycles) (Illumina #20024906) in a single read mode.
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bioRxiv - Synthetic Biology 2022Quote: ... using Qiagen RNeasy Plus kit.200ng of extracted RNA was used to produce SARS-CoV-2 amplicon libraries using the NEBNext SARS-CoV-2 FS Library Prep Kit (Illumina) using the VarSkip Short Express Protocol with 25 minutes fragmentation step and 8 cycles of PCR enrichment ...
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bioRxiv - Pathology 2022Quote: ... PCR-mediated adaptor addition and amplification of libraries was performed using the Nextera library preparation protocol (Nextera XT DNA Library Preparation kit, Illumina), as described51 ...
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bioRxiv - Molecular Biology 2022Quote: ... MEFs and NPCs were used to prepare NGS libraries based on the NEB Next Ultra II directional RNA library preparation kit from Illumina. As default ...
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bioRxiv - Microbiology 2022Quote: ... After DNAse treatment 50ng-1ug of total RNA was used to prepare DNA libraries with Truseq RNA Library Prep Kit v2 (Illumina) following manufacture’s instruction ...
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bioRxiv - Microbiology 2022Quote: ... 1 ug of total RNA from each tissue was enriched for polyadenylated RNA and prepared for next-generation sequencing using the TruSeq Stranded mRNA Library Prep Kit (Illumina) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... The viral spike sequences were verified using unbiased NGS sequencing (KAPA HyperPrep library kit with RiboErase, followed by Illumina sequencing). All cells and virus stocks tested negative for mycoplasma using MycoAlert Plus reagents (Lonza) ...
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bioRxiv - Genomics 2022Quote: ... A library was prepared using a TruSeq DNA PCR-Free library preparation kit according to manufacturer guidance and was sequenced on a NextSeq500 instrument (Illumina) with a NextSeq High Output v2 300 cycle kit ...
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bioRxiv - Genomics 2022Quote: Whole-Genome sequencing libraries were prepared from 250 ng of genomic DNA by tagmentation with the NexteraFlex kit from Illumina. Subsequently ...
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bioRxiv - Microbiology 2022Quote: ... Illumina sequencing libraries were prepared using the Nextera XT sample prep kit and sequenced on the Illumina MiSeq platform (Illumina) with 300-bp paired-end read configuration (MiSeq Reagent Kit v3) ...
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bioRxiv - Microbiology 2022Quote: ... Messenger RNA was enriched by first depleting ribosomal RNA from samples using the Ribo-Zero rRNA removal (bacteria) magnetic kit (Illumina). The purified mRNA was prepared for Illumina sequencing using the NEBNext Ultra RNA Library Prep Kit for Illumina according to the manufacturer’s instructions in combination with AMPure XP reagent for clean-up of adaptor-ligated DNA ...
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bioRxiv - Microbiology 2022Quote: ... RNAseq libraries were prepared from total RNA using the Nextera DNA Flex library preparation kit (Illumina, Inc., San Diego, CA) according to the manufacturer’s instructions where the first-strand cDNA was synthesized using SuperScript II Reverse Transcriptase (Thermo Fisher ...
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bioRxiv - Genomics 2022Quote: ... Illumina library prep was performed using Nextera DNA Flex Library Preparation kit (cat no. 20018704) and sequenced on the iSeq100 (Illumina).
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bioRxiv - Neuroscience 2022Quote: ... and finally converted into DNA libraries using custom rpi primers (RNA PCR Primer Index) adapted from the Truseq Small RNA kit (Illumina)106 ...
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bioRxiv - Genomics 2022Quote: ... A paired-end library was prepared with the TruSeq DNA PCR-Free LT Sample Preparation Kit (Illumina, #FC-121-3001) from 1 µg of the genomic DNA ...
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bioRxiv - Microbiology 2022Quote: ... Further library preparation of rRNA-depleted samples was performed using TruSeq Stranded mRNA library preparation kit (Illumina, RS-122-2101) according to the manufacturer’s instructions apart from omitting the initial steps for mRNA pull-down ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Sequencing was performed on a MiSeq system with the reagent kit v3 with 600 cycles (Illumina, San Diego, CA, USA) as recommended by the manufacturer and resulted in a minimum average coverage of 88× per strain (coverage range was from 88× to 157×) ...
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bioRxiv - Cell Biology 2022Quote: ... RNA library was constructed with 10x-Genomics Single Cell 3’ RNA sequencing kit v3 as previously described(18) sequenced using a NextSeq500 (Illumina). Cell clusters were characterized using an automated annotation tool(19 ...