Labshake search
Citations for Illumina :
6601 - 6650 of 8781 citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: Sequencing libraries were made according to the Illumina standardized protocol using the Nextera XT DNA sample preparation kit (Illumina Inc.). All DNA samples were quantitated by Qubit HS (ThermoFisher Scientific ...
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bioRxiv - Cancer Biology 2021Quote: ... Approximately 500 ng of total RNA was used as input for library preparation using TruSeq RNA Sample Preparation Kit v2 (Illumina).
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bioRxiv - Immunology 2021Quote: ... cDNA concentrations were normalised and 0.125 ng of each cDNA was used in a quarter volume of a Nextera XT DNA library preparation kit (Illumina), following pooling of equal volumes from each well and purification with 0.6x AMPure XP SPRI beads ...
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bioRxiv - Genetics 2020Quote: ... Transposition reaction was performed according the manufacturer’s protocol for Nextra Tn5 transpoase Nextra kit (Illumina, Cat. No.: FC-121-1030). Transposed DNA fragments were purified by Qiagen MiniElute PCR purification kit (Qiagen) ...
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bioRxiv - Immunology 2020Quote: ... Two hundred ng of total RNA were used to generate libraries using the TruSeq Stranded mRNA Sample Preparation Kit (Illumina). Single-end reads of 100bp for each sample were produced with Illumina’s HiSeq2500v4 instrument ...
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bioRxiv - Immunology 2020Quote: ... Single cell RNA libraries were prepared according to the 10x Genomics Chromium Single Cell 3’ Reagent Kits v2 User Guide and sequenced on a HiSeq4000 (Illumina).
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bioRxiv - Genomics 2021Quote: ... cells and CD14+ monocytes were generated using the SureCell WTA 3′ Library Prep Kit for the ddSEQ System (20014280, Illumina) with the following modifications ...
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bioRxiv - Genomics 2021Quote: ... RNA libraries were prepared for sequencing by the Cancer Genomics Shared Resource (Wake Forest School of Medicine, Winston-Salem, NC) using the TruSeq-stranded total RNA kit (Illumina), which includes a ribosomal depletion step ...
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bioRxiv - Genomics 2021Quote: ... The samples were amplified for 5-8 cycles as determined by qPCR for Illumina sequencing using Nextera library preparation kit (Illumina) and samples were paired-end sequenced on HiSeq4000 ...
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bioRxiv - Genetics 2020Quote: ... Ribosomal RNA was subtracted by hybridization from total RNA samples using the RiboZero Magnetic Gold H/M/R Kit (Illumina) and the rRNA-subtracted samples were quantified with a Qubit 2.0 (RNA HS Kit ...
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bioRxiv - Genetics 2021Quote: ... one 150 bp paired end (350 bp insert size) Illumina library was prepared from the same DNA (NEBNext Ultra II DNA Library Prep Kit for Illumina) and sequenced to approximately 140 fold coverage (24.6 Gb ...
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bioRxiv - Evolutionary Biology 2020Quote: ... 250 ng of purified genomic DNA was used from each sample for library preparation using the Nextera DNA Flex Library Prep Kit (Illumina) per the manufacturer’s protocols ...
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bioRxiv - Genetics 2021Quote: ... 700 ng of genomic DNA was sheared to an average size of 400 bp using a Covaris LE220 and was used as input to generate a whole-genome library using the TruSeq PCR-free kit (Illumina). The resulting DNA libraries were sequenced on Illumina Hi-seq X instrument to generate 2×150bp paired-end reads.
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Nonsense Mediated RNA Decay Is a Unique Vulnerability of Cancer Cells with SF3B1 and U2AF1 MutationsbioRxiv - Cell Biology 2021Quote: ... RNA-sequencing libraries were then prepared from RNA isolated from the bulk mutant AML bone marrow cells and the normal CD34+ bone marrow cells using TruSeq Stranded Total RNA kit (Illumina) described above ...
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bioRxiv - Cell Biology 2021Quote: ... and then depleted of rRNAs and processed for RNA-Seq library construction using a TruSeq Stranded mRNA Library Prep Kit (Illumina). RNA-Seq data were acquired on an Illumina Hiseq 4000 sequencer ...
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bioRxiv - Genomics 2021Quote: ... Genomic DNA of 17 new isolates and 14FG141 and Mur_S3 of the previous 141 were extracted using a Qiagen DNeasy Plant Mini kit (Venlo, Netherlands. Catalogue ID: 69104) and sequenced by Novogene (Beijing, China) (Illumina HiSeq2500 ...
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bioRxiv - Genomics 2021Quote: ... and each sample was uniquely indexed using Nextera XT Index Kit V2 indexing primers (Illumina, Cat. No. FC-131-2001). Indexed libraries were purified with Omega Mag-Bind Total Pure NGS Beads ...
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bioRxiv - Genomics 2021Quote: The read sequences in FASTQ format were first trimmed by removing six bases from random primers of TruSeq DNA Methylation Kit (Illumina) and the last 50 bases of low-quality reads ...
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bioRxiv - Genomics 2021Quote: ... Bisulfite-treated DNA samples (50 ng) were subjected to 10 cycles of PCR using random primers from the TruSeq DNA Methylation Kit (Illumina) to add adapters ...
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bioRxiv - Physiology 2021Quote: ... Single-indexed strand-specific cDNA library from total RNA samples (500 ng input with RIN ≥ 5) was prepared using TruSeq Stranded mRNA Library Prep Kit (Illumina) as per directions by the manufacturer ...
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bioRxiv - Physiology 2021Quote: ... Poly-A enriched complementary DNA (cDNA) libraries were constructed using the TruSeq RNA Sample Preparation Kit (Illumina; San Diego, USA). Briefly ...
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bioRxiv - Physiology 2021Quote: ... were clustered and amplified on cBot using HiSeq 3000/4000 PE Cluster Kit and sequenced (2×75 bp paired-end reads) on HiSeq4000 (Illumina) using HiSeq 3000/4000 PE SBS Kit ...
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bioRxiv - Plant Biology 2021Quote: ... RNA libraries were prepared using ~950 ng of the DNAase treated total RNA using the NEBNext Ultra Directional RNA Library Prep Kit (Illumina) and size selected for 200bp insert size ...
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bioRxiv - Microbiology 2021Quote: Extracted DNA was prepared for sequencing by the University of Minnesota Genomics Center (UMGC) using the Nextera XT DNA Library Prep Kit (Illumina). Sequencing of the libraries was performed by UMGC on an Illumina NovaSeq (2 × 150 bp reads) ...
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bioRxiv - Physiology 2020Quote: ... single-cell barcoded cDNA libraries were generated with 10X Chromium preparation system using the Chromium Single Cell 3’ Library & Gel Bead Kit v3 and sequenced by Illumina HiSeq ...
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bioRxiv - Microbiology 2021Quote: ... 1.5 μg of purified RNA for each sample was processed using the Ribozero rRNA Removal Kit (Gram-negative bacteria) (Illumina) according to the manufacturer’s instructions except that reaction volumes were reduced by 50% ...
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bioRxiv - Microbiology 2020Quote: ... The obtained partitions were further processed using the Chromium Single Cell 3’ Reagent Kit (version 2, 10x Genomics) to generate Nextera XT sequencing libraries that were sequenced on a HiSeq3000 (Illumina), using a single flow cell lane for each library.
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bioRxiv - Neuroscience 2021Quote: ... Next-generation sequencing (NGS) libraries were prepared using 150 pg of cDNA input in the Nextera XT DNA Library Preparation Kit (Illumina), followed by 12 cycles of PCR ...
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bioRxiv - Genomics 2021Quote: ... sequencing libraries from each extraction were prepared using the DNA Prep kit and indexed using DNA/RNA UD indexes (Illumina), and subsequently subjected to paired-end sequencing on either an Illumina MiSeq® or NextSeq 2000® instrument ...
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bioRxiv - Plant Biology 2020Quote: ... RNA was processed by the Technion Genome Center as following: RNA libraries were prepared using TruSeq RNA Library Prep Kit v2 (Illumina), according to manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2021Quote: ... and subjected to a second PCR to introduce dual index sequences using Nextera XT Index Kits (Illumina, San Diego, CA). In the second PCR ...
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bioRxiv - Cell Biology 2020Quote: ... RNA-seq libraries were prepared using the Illumina TruSeq stranded mRNA library preparation kit and sequenced on HiSeq4000 sequencer (Illumina). RNA-seq data were aligned to the mm9 reference genome using STAR 2.3.0e61 ...
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bioRxiv - Cell Biology 2020Quote: ... denatured and diluted to 10 pM with pre-chilled hybridization buffer and loaded into TruSeq PE v3 flowcells on an Illumina cBot followed by indexed paired-end sequencing (101 + 7 + 101 bp) on an Illumina HiSeq 2000 using TruSeq SBS Kit v3 chemistry (Illumina). Paired de-multiplexed fastq files were generated using CASAVA software (Illumina ...
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bioRxiv - Evolutionary Biology 2021Quote: ... except for two adult gibbosus and tuberosus males for which the non-strand-specific TruSeq Total RNA with Ribo-Zero kit (Illumina) was used ...
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bioRxiv - Evolutionary Biology 2020Quote: ... and mate-pair libraries of 3 kb insert size were prepared using the Nextera Mate Pair Sample Preparation Kit (cat. No. FC-132-1001, Illumina). We then assessed library quality using the HS DNA Kit (Agilent ...
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bioRxiv - Microbiology 2021Quote: ... the libraries were clustered on a cBot Cluster Generation System using a TruSeq PE Cluster Kit v3-cBot-HS (Illumina) and sequenced using an Illumina NovaSeq 6000 System.
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bioRxiv - Microbiology 2021Quote: ... A second PCR reaction was performed according to the manufacturers protocol to obtain sequence libraries with adaptor sequences from the TruSeq DNA LT Sample Prep Kit (Illumina). PCR products were purified using magnetic Agencourt AMPure XP beads (Beckman Coulter ...
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bioRxiv - Developmental Biology 2021Quote: Libraries were prepared for 150 bp paired-end sequences using a TruSeq Stranded mRNA Sample Preparation Kit (Illumina, CA, USA). Namely ...
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bioRxiv - Cell Biology 2021Quote: Libraries were prepared with TruSeq Stranded Total Library preparation kit (ref 20020598) according to supplier recommendations (Illumina, San Diego, CA). Briefly ...
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bioRxiv - Microbiology 2021Quote: ... cDNA library was prepared by using NEB Next Ultra RNA Library Prep Kit for Illumina (New England Biolab, Cat# E7530) and whole genome sequencing was performed by Miseq (Illumina).
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bioRxiv - Genomics 2021Quote: ... of Korea) for library preparation with 350 bp targeted insert size using TruSeq DNA PCR Free preparation kit (Illumina, USA) and sequencing on HiSeq X device (Illumina ...
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bioRxiv - Immunology 2021Quote: ... Sequencing was performed in the Human Immunology Core Facility at the University of Pennsylvania using an Illumina 2× 300-bp paired-end kit (Illumina MiSeq Reagent Kit v3 ...
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Enhancer Remodeling Promotes Tumor-Initiating Activity in NRF2-Activated Non-Small Cell Lung CancersbioRxiv - Cancer Biology 2020Quote: ... 4 μg of total RNA from A549 cells was subjected to rRNA removal using the Ribo-Zero Gold kit (Illumina). cDNA sequencing libraries were then prepared using the SureSelect Strand-Specific RNA library preparation kit (Agilent Technologies ...
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bioRxiv - Neuroscience 2021Quote: ... Pooled sequencing libraries were loaded at a concentration of 1.8pM with 10% PhiX spike-in and sequenced using eight Illumina NextSeq 150 Cycle Hi-Output v2.5 kits (Illumina #20024907) on an Illumina NextSeq 550 System ...
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bioRxiv - Neuroscience 2021Quote: Sequencing of the pooled libraries was completed by the NIDDK Genomics Core on an Illumina NextSeq 550 system using a NextSeq 150 Cycle Hi-Output v2.5 kit (Illumina #20024907), generating a total of 400 million reads for an estimated sequencing depth of 40,000 reads per cell ...
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bioRxiv - Neuroscience 2021Quote: ... Libraries were base-pair selected based on Agilent 2100 Bioanalyzer profiles and normalized determined by KAPA Library Quantification Kit (Illumina). The libraries were built from 5 different mice per genotype ...
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bioRxiv - Neuroscience 2021Quote: ... We fragmented the rRNA-depleted RNA samples and processed them into strand-specific cDNA libraries using TruSeq Stranded Total LT Sample Prep Kit (Illumina) and then sequenced them on NextSeq 500 ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Subsequent to second round PCR amplification MiSeq sequencing libraries were generated using Nextera XT DNA Sample Preparation Kits (Illumina, Inc.). Amplicons for each sample were tagged using Nextera XT Index Kits (Illumina ...
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bioRxiv - Genetics 2021Quote: ... and ribosomal RNA was depleted from 5 ug of total RNA using the Ribo-Zero™ Gold Kit (Illumina, Inc.). Depleted mRNA was fragmented and converted to first strand cDNA ...
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bioRxiv - Plant Biology 2021Quote: ... using 10 mM of pooled normalized libraries on the cBOT with a TruSeq PE Cluster Kit v3-cBot-HS (Illumina). Subsequently Illumina HiSeq 4000 sequencing was performed to generate the reads.