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Citations for Illumina :
601 - 650 of 890 citations for GDNF family receptor alpha 1 GFRA1 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: ... The chromatin was then tagmented by resuspending beads in 29 µl Tagmentation Buffer (10 mM Tris-HCl pH 8.0, 5 mM MgCl2, 10% dimethylformamide) and adding 1 µl of transposase (Illumina). Samples were incubated at 37 °C for 10 min and the reaction was terminated by adding 150 µl RIPA buffer ...
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bioRxiv - Genetics 2022Quote: ... sequence reads were mapped to the mouse reference genome GRCm38/mm10 (iGenomes, Illumina; chromosomes 1-19, X, Y, M) using the Rsubread v1.28.1 package in R v3.4.4 ...
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bioRxiv - Pathology 2019Quote: ... NGS libraries were prepared from 1 ng of extracted DNA using Nextera®XT DNA Sample Preparation Kit (Illumina), and sequencing was performed on an Illumina MiSeq sequencer using MiSeq Reagent Kit v2 (500-cycles ...
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bioRxiv - Cell Biology 2019Quote: ... cDNA libraries were prepared using 1 µg of total RNA using the TruSeq RNA Sample Preparation Kit v2 (Illumina). RNA quality was assessed by an Agilent Bioanalyzer 2100 ...
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bioRxiv - Systems Biology 2019Quote: ... Washed AMPure XP beads attached to double-stranded cDNAs were directly eluted using 3.75 μL of 1× diluted Tagment DNA Buffer (Illumina) and mixed well using a vortex mixer and pipetting ...
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bioRxiv - Genetics 2020Quote: ... for PPI (see supplementary methods).14 The 148 additionally selected SNV markers (supplementary table 1) were genotyped in 1,012 F2 mice by Illumina BeadArray genotyping (Illumina Golden Gate assay ...
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bioRxiv - Synthetic Biology 2021Quote: ... cDNA was diluted to an average of 200 pg/µl and 100 pg cDNA from each cell was tagmented by adding 1 µl TD and 0.5 µl ATM from a Nextera XT DNA Library Preparation Kit (Illumina) to 0.5 µl diluted cDNA in each well of a fresh 384-well plate ...
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bioRxiv - Immunology 2020Quote: ... RNA-Seq libraries were prepared using 1 μg of end-repaired cDNA using the TruSeq Stranded RNA Kit (Illumina), however due to the method of amplification the libraries were not stranded ...
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bioRxiv - Cancer Biology 2019Quote: ... Paired-end sequencing libraries were prepared using 1 μg of purified RNA following the mRNA-Seq Sample Prep Kit according to the manufacturer’s instructions (Illumina). RNA-Seq libraries were sequenced on two lanes each of an Illumina GAIIx Genome Analyser to a minimum depth of 49 million reads ...
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bioRxiv - Genomics 2021Quote: ... 30 μL of NEBNext Ultra II Ligation Master Mix, 1 μL of NEBNext Ligation Enhancer, 2.5 μL of NEBNext Adapter for Illumina) at 20 °C for 15 min. ...
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bioRxiv - Developmental Biology 2022Quote: ... The PCR product was purified in a 1 % agarose gel and sequenced using a NextSeq 550 and NovaSeq (Illumina) to obtain 79+79-nt ...
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bioRxiv - Plant Biology 2022Quote: The triplicate total RNA samples used for single-end 1×75bp RNA-Seq on a NextSeq500 sequencing platform (Illumina). After examining the data quality using FastQC (v0.11.5) ...
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bioRxiv - Genomics 2022Quote: ... 5 μl of Index primer (both provided in NEBNext Multiplex Oligos for Illumina Index Primers Sets 1 and 2), and 25 μl NEBNext Ultra II Q5 Master Mix (PCR cycling conditions ...
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bioRxiv - Plant Biology 2023Quote: ... The library was diluted to 0.5 nM with EB in 18 μL and spiked with 1% PhiX Control v3 (Illumina) before being prepared for loading using a NovaSeq XP 2 lane kit v1.5 (20043130 ...
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Resolution of SARS-CoV-2 infection in human lung tissues is driven by extravascular CD163+ monocytesbioRxiv - Immunology 2024Quote: ... All cDNA libraries were sequenced on an Illumina NextSeq 500 instrument at the Boston University microarray and sequencing core according to Illumina and 10x Genomics guidelines with 1.4-1.8pM input and 1% PhiX control library spike-in (Illumina, USA).
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bioRxiv - Microbiology 2022Quote: ... RNA-Seq libraries were prepared using 1 ug of total RNA with TruSeq Stranded mRNA Library Prep kit (Illumina) following the manufacturer’s instructions ...
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bioRxiv - Pathology 2023Quote: ... All cDNA libraries were sequenced on an Illumina NextSeq 500 instrument according to Illumina and 10X Genomics guidelines with 1.4–1.8 pM input and 1% PhiX control library spike-in (Illumina, USA). Sequencing data were processed using 10X Genomics’ Cell Ranger pipeline to generate feature/barcode matrices from raw count data.
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bioRxiv - Developmental Biology 2023Quote: ... bead-bound complexes were resuspended in tagmentation mixture (25µL reaction containing 1 µL of enzyme in 1X buffer, Illumina) and incubated at 37°C for 25minutes ...
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bioRxiv - Developmental Biology 2023Quote: ... Sequencing libraries were prepared using 1 μg of total RNA with the TruSeq Stranded mRNA Sample Prep Kit (Illumina) and the TruSeq single-index adaptor (Illumina) ...
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bioRxiv - Pathology 2023Quote: ... Barcoded libraries from each experimental sample were combined in equimolar concentrations of 1.5 pM prior to sequencing at 75bp x 1 (single-end) read metric on a NextSeq 550 (Illumina) system.
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bioRxiv - Immunology 2023Quote: ... The samples were sequenced in a paired-end run (read 1: 28bp, read 2: 91bp) on a NovaSeq6000 platform using S1 v1.5 (100 cycles) sequencing kits (Illumina). Bcl2fastq software (v2.20.0.422 ...
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bioRxiv - Immunology 2022Quote: ... 5,000-50.000 cells per experiment were centrifuged 5 minutes at 8°C, and resuspended in 25 µl transposase mixture (12.5 µl Tagmentation DNA buffer, 1 µl Tn5 transposase (Illumina), 10.75 µl nuclease-free water ...
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bioRxiv - Microbiology 2023Quote: ... 1 ng of DNA was used to prepare DNA libraries using the Nextera XT DNA Library Preparation Kit (Illumina) and genomic DNA was fragmented with Illumina Nextera XT fragmentation enzyme ...
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bioRxiv - Molecular Biology 2024Quote: ... 1 μg RNA and a TruSeq RNA Library Prep Kit v2 (RS-122-2001, Illumina, San Diego, CA, USA) were used ...
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bioRxiv - Cancer Biology 2023Quote: ... 1 ng DNA was used to generate the sequencing library by using Nextera XT DNA Library Preparation Kit (Illumina).
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bioRxiv - Bioengineering 2023Quote: ... 10x libraries were pooled and charged with 1% PhiX on one SP lane of the NovaSeq 6000 instrument (Illumina) using the NovaSeq 6000 SP Reagent Kit v1.5 Raw sequencing data of the modules viability study were processed with Cellranger80 ...
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bioRxiv - Molecular Biology 2023Quote: ... Pelleted nuclei were incubated in transposase reaction mix (1 X TD reaction buffer, 2.5 µl Tn5 transposase (Nextera, Illumina)) for 30 min at 37 °C ...
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bioRxiv - Developmental Biology 2024Quote: ... The mRNA-Seq libraries were prepared with 1 μg of total RNA using the TruSeq RNA Library Prep Kit v2 per the manufacturer’s instructions (Illumina). Three independent biological replicates were prepared for each condition and libraries were sequenced on an Illumina HiSeq 4000 instrument (single-end ...
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bioRxiv - Genomics 2024Quote: The read 1 fastq file from each spatial region was mapped on the mm10 reference genome (source: Illumina igenomes) using the STAR package (v 2.7.9a ...
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bioRxiv - Cell Biology 2024Quote: ... we prepared the mRNA-Seq libraries using 1 μg of total RNA and the TruSeq RNA Library Prep Kit v2 according to the manufacturer’s instructions (Illumina). The samples were sequenced on an Illumina HiSeq 4000 instrument (single-end ...
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bioRxiv - Genomics 2024Quote: ... We added a FORMAT (GT) and SAMPLE (0/1) column to the VCF and used hap.py (https://github.com/Illumina/hap.py) to compare the v4.2.1 benchmark VCF and BED (https://ftp-trace.ncbi.nlm.nih.gov/ReferenceSamples/giab/release/ChineseTrio/HG005_NA24631_son/NISTv4.2.1/GRCh38/) ...
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bioRxiv - Cell Biology 2020Quote: ... RNA-Seq libraries were generated from 1 μg of total RNA from duplicated samples per condition using the TruSeq LT RNA Library Preparation Kit v2 (Illumina) following the manufacturer’s protocol ...
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bioRxiv - Genetics 2021Quote: ... Libraries were sequenced by NextSeq 550 High Output with 21 bp for read 1 and 72 bp for read 2 (Illumina). Sequenced reads were aligned using STAR (v2.7.6a ...
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bioRxiv - Genetics 2021Quote: ... The scRNAseq samples were then sequenced on 1/7th of a single S4 lane on a NovaSeq 6000 platform (Illumina). For the ScISOr-Seq library ...
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bioRxiv - Microbiology 2020Quote: ... pooled with 1% denatured PhiX DNA (sequencing control) and sequenced in a MiSeq platform (2× 251 cycles paired-end run; Illumina). New PCR reactions using combinations of the primers described above were carried out to cover regions with low coverage for each sample ...
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bioRxiv - Microbiology 2019Quote: Two overlapping IN-PCR regions corresponding to the 288 amino acids of HIV-1 IN were sequenced with the MiSeq NGS platform (Illumina). The amplicons were purified with Agencourt AMPure XP (Beckman Coulter ...
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bioRxiv - Microbiology 2019Quote: Total RNA (1 µg) from hASCs was used to construct sequencing libraries with the Truseq Stranded Total RNA LT Kit (Illumina). Quality of RNA and constructed libraries was determined via 2100 Bioanalyzer ...
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bioRxiv - Developmental Biology 2021Quote: ... 10%(v/v) N,N-dimethyl formamide) and 1 μl of Tagment DNA Enzyme from Nextera Sample Preparation Kit (Illumina) were added to the DNA-beads complex and incubated for 70 sec at 37 °C ...
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bioRxiv - Developmental Biology 2020Quote: ... Libraries were generated using Kapa Biosystems library preparation kit (#KK8201) and multiplexed libraries were sequenced on a 1×75 High output flow cell on the NextSeq550 platform (Illumina). Reads were filtered and trimmed to remove adapter-derived or low-quality bases using Trimmomatic and checked again with FASTQC ...
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bioRxiv - Genomics 2020Quote: ... Other libraries were sequenced on NextSeq 500 (1x 28 / 1×91 cycles plus 8 base index cycle) using the v2 150 cycle High Output kit (Illumina) as per the manufacturer’s instructions ...
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bioRxiv - Genetics 2019Quote: The accuracy of the variant calls in the filtered dataset was evaluated in two ways: 1) the concordance of genotype calls by Illumina sequencing and by TaqMan allelic discrimination assays (described in Marini et al. ...
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bioRxiv - Genomics 2019Quote: Libraries were prepared using 1 µg of total RNA in the randomized order using TruSeq RNA Sample Prep Kit v2 (Illumina) to generate cDNA as per manufacturer’s instructions using adapters from both Box A and Box B ...
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bioRxiv - Cell Biology 2019Quote: A minimum of 1 µg total RNA in 50 µl was used for library preparation using the TruSeq RNA sample preparation kit (Illumina) following the manufacturer’s protocol at Novogene ...
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bioRxiv - Genetics 2020Quote: ChIP-seq and DNase-seq libraries were prepared from 1-5 ng ChIP DNA or DNase DNA samples using NEBNext Ultra II DNA library Prep Kit (Illumina). Libraries were sequenced on an Illumina NextSeq 500 with single-end or paired-end 75 bp reads.
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bioRxiv - Neuroscience 2019Quote: ... We pooled and sequenced the libraries with paired-end reads (50 bases for read 1 and 25 bases for read 2) on two flowcells with a NextSeq 500 instrument (Illumina).
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bioRxiv - Genomics 2019Quote: ... two different strategies were implemented in order to help in genome scaffolding: 1) Nextera Mate-Pair Preparation Kit (Illumina Inc) was used to make two mate pairs (MP ...
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bioRxiv - Microbiology 2019Quote: ... Amounts of 1 ng cDNA were used to prepare NGS sequencing libraries using Nextera XT DNA Library Prep Kit (Illumina), with unique index adaptors for each sample ...
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bioRxiv - Genomics 2019Quote: ... and marker-wise (HWE P value > 1 × 10−6, call rate > 95%, and for the GSA array additionally by Illumina GenomeStudio GenTrain score > 0.6 ...
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bioRxiv - Systems Biology 2020Quote: ... then performed paired-end sequencing of these libraries (38 cycles read 1 + 37 cycles read 2) on a NextSeq 500 (Illumina). After sequencing ...
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bioRxiv - Immunology 2020Quote: ... cDNA libraries were prepared from 1 μg of total RNA using the TruSeq Stranded mRNA kit (Illumina, San Diego, CA) and the Sciclone NGSx Workstation (Perkin Elmer ...