Labshake search
Citations for Illumina :
551 - 600 of 933 citations for NCR1 Mouse HEK293 Fc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Genomics 2022Quote: Tn5 integration was performed as previously published 90 on purified nuclei using the Nextera Illumina kit (Illumina, FC 121 1031) at 37 °C for 30 min ...
-
bioRxiv - Neuroscience 2022Quote: ... Each pool was sequenced on the Illumina NextSeq 500 after the addition of 5% PhiX sequencing control library (FC-110-3002; Illumina) using the following settings ...
-
bioRxiv - Molecular Biology 2022Quote: ... MCF7 and BT-474 libraries were independently amplified with index primers N7xx and S5xx of Nextera XT Index Kit v2 Set A (FC-131-2001, Illumina) and Nextera XT Index Kit v2 Set B (FC-131-2002 ...
-
bioRxiv - Neuroscience 2023Quote: ... mRNA-seq libraries were prepared using Smart-seq2 (Picelli et al., 2013) and a Nextera XT DNA Library Preparation kit (FC-131-1024, Illumina) following the protocol and the manufacturer’s instructions with minor modification ...
-
bioRxiv - Cancer Biology 2024Quote: Sequencing libraries were constructed from the cDNA using the Illumina Nextera XT DNA Library Preparation Kit (Illumina, FC-131-1096). The cDNA library was sequenced on a NovaSeq 6000 instrument using 150-bp paired-end reads.
-
bioRxiv - Evolutionary Biology 2024Quote: ... sequencing libraries were prepared from 1μg DNA using the TruSeq PCRfree DNA sample preparation kit (cat# FC-121-3001/3002, Illumina Inc.). The library preparation was performed according to the manufacturers’ instructions (guide#15036187).
-
bioRxiv - Developmental Biology 2024Quote: ... Libraries for sequencing were prepared from 600pg cDNA input using Nextera XT library preparation kit (Illumina, Cat: FC-131-1096) with i7 primers from Illumina (N70X ...
-
bioRxiv - Pathology 2024Quote: ... and 2ng of each sample was used to construct a pool of uniquely indexed samples (Illumina Cat# FC- 131-1096). A second amplification was performed with 12 cycles and cleaned up with AMPure XP beads ...
-
bioRxiv - Cancer Biology 2023Quote: ... in single-end mode using the NextSeq 500/550 High Output Kit (75 Cycles) (Illumina, cat. no. FC-404-2005) except for scCUTseq libraries from brain and skeletal muscle ...
-
bioRxiv - Molecular Biology 2023Quote: ATAC-seq was performed based on published protocols 83 using reagents from a Nextera sequencing kit (Illumina, FC-121-1030). Cells were detached using TrypLE Express (Gibco ...
-
bioRxiv - Molecular Biology 2023Quote: Selected FLIPS proviral amplicons underwent NGS library preparation using the Nextera XT DNA Library Preparation Kit (Illumina, #FC-131-1096) with indexing of 96-samples per run according to the manufacturer’s instructions (Illumina ...
-
bioRxiv - Genomics 2023Quote: ... The PhiX Control library was spiked in the sequencing sample at 10% (v/v) (Illumina, cat. no. FC-110-3001).
-
bioRxiv - Genetics 2023Quote: ... The nuclei was resuspended in the transposition reaction mix containing 25 μL 2x TD Buffer (Illumina, cat #FC-121-1030), 2.5 μL Tn5 Transposase (Illumina ...
-
bioRxiv - Microbiology 2023Quote: ... a PhiX spike-in of 2.5–5% was added to the pools (PhiX sequencing control v3; Illumina FC-110- 3001). Samples were run on the Illumina NovaSeq 6000 platform (single-read 1 ×85 cycles and 6 × i7 index cycles).
-
bioRxiv - Immunology 2023Quote: ... Paired-end sequencing libraries were prepared from amplified cDNA with the Nextera XT DNA sample Prep Kit (Illumina, #FC-131) according to the manufacturer’s instructions ...
-
bioRxiv - Physiology 2023Quote: ... mRNA-Seq libraries were then prepared using the Illumina Nextera XT DNA Library Preparation kit (Illumina Cat # FC-131-1024). 1 ng of cDNA was used as input and 12 amplification cycles were used during PCR enrichment ...
-
bioRxiv - Microbiology 2023Quote: ... All sequencing runs were performed with a spike-in of 1% PhiX control library V3 (Illumina, Cat. FC-110-3001). The sequencing mean library size was 134,629,540.5 reads [range ...
-
bioRxiv - Biochemistry 2024Quote: ... San Francisco Genomics CoLab using a Miniseq high output 150 cycles kit for paired end reads (Illumina FC-420-1002). Samples were demultiplexed and analysis performed using the University of California ...
-
bioRxiv - Bioengineering 2024Quote: ... The NGS library was run on an Illumina MiniSeq using a 300-cycle mid output kit (Illumina, FC-420-1004). Editing was quantified using CRISPResso2 in standard mode ...
-
bioRxiv - Synthetic Biology 2024Quote: ... and whole plasmid sequences were verified using either the Nextera XT DNA library prep kit (Illumina, Catalog #FC-131-1096) or whole plasmid sequencing services (Plasmidsaurus Inc. ...
-
bioRxiv - Cell Biology 2020Quote: ... Tagmentation was performed during 30 minutes at 25°C in a 50 μl reaction in TD Buffer (Illumina, FC-121-1030) containing 2.5 μl Tn5 Transposes (Illumina ...
-
bioRxiv - Molecular Biology 2021Quote: ... The crude nuclear prep was then centrifuged and resuspended in 1x TD buffer containing the Tn5 transposase (Illumina FC-121-1030). The transposition reaction was incubated at 37C for 30 minutes and immediately purified using the Qiagen MinElute kit ...
-
bioRxiv - Microbiology 2021Quote: ... Both mRNA and vRNA samples were fragmented by tagmentation using the Nextera XT DNA library preparation kit from Illumina (FC-131), indexed ...
-
bioRxiv - Neuroscience 2020Quote: ... Multiplex samples Pool (1.5pM including PhiX 1.5%) was loaded in NextSeq 500/550 High Output v2 kit (75 cycles) cartridge (Illumina; FC-404-1005) and loaded on NextSeq 500 System machine (Illumina) ...
-
bioRxiv - Molecular Biology 2020Quote: Deep sequencing was carried out on an Illumina NextSeq using commercially available kits from Illumina (Danyel Biotech, Cat #FC-404-2005) following the manufacturer’s protocols.
-
bioRxiv - Molecular Biology 2020Quote: ... Illumina sequencing libraries were prepared according to the protocol in the Nextera XT Library Sample Preparation kit (Illumina, FC-131-1096). Each well was mixed with 0.8 μl Nextera tagmentation DNA buffer (Illumina ...
-
bioRxiv - Genomics 2020Quote: ... 25 μL TD buffer and 2.5 μL TDE1 enzyme from Nextera DNA Library Prep Kit (Illumina, cat. no. FC-121-1030)) ...
-
bioRxiv - Microbiology 2019Quote: ... Illumina recommended denaturation and loading recommendations which included a 1% PhiX spike in (PhiX Control v3 Illumina Catalogue FC-110-3001). Whole genome sequencing data has been deposited in the Sequence Read Archive under PRJNA529870.
-
bioRxiv - Genetics 2019Quote: ... MS-103-2003) at a concentration of 15 nM with an addition of 25% Phix Control v3 (Illumina, FC-11-2003).
-
bioRxiv - Systems Biology 2019Quote: ... 2ng of the amplified cDNA was converted into sequencing library with the Nextera XT DNA Library kit (Illumina, FC-131-1024), according to supplied protocol ...
-
bioRxiv - Neuroscience 2019Quote: ... The diluted libraries were then loaded onto a reagent cartridge and forwarded to a sequencing run on the Illumina NextSeq 500 system (RRID: SCR_014983) by using a NextSeq 500/550 V2.5 kit (Illumina, USA, #FC-404) in accordance with manufacturer’s instructions ...
-
bioRxiv - Cancer Biology 2020Quote: ... TN5 tagmentation and library amplification realized 3’end fragments by Nextera XT DNA Sample Preparation Kit (Illumina, Cat#FC-131-1024) according to the manufacturer’s instructions while P5_TSO and Nextera_N7xx took the place of the custom primers ...
-
bioRxiv - Physiology 2021Quote: ... cDNA was subsequently tagmented and amplified for 12 cycles by using a Nextera XT DNA Library Preparation Kit (Illumina FC-131). Sequencing libraries were analyzed with Qubit and Agilent Bioanalyzer ...
-
bioRxiv - Cancer Biology 2020Quote: ... Samples were cleaned using Sample Purification Beads from the TruSeq Methyl Capture EPIC LT Library Prep Kit (Illumina, #FC-151-1002) according to the manufacturer’s instructions with modifications ...
-
bioRxiv - Neuroscience 2022Quote: ... Nuclei were pelleted (500xg, 10 min, 4 °C) and tagmented with the Nextera DNA Library Prep Kit (Illumina, FC-121-1030) for 1 h at 37 °C ...
-
bioRxiv - Microbiology 2020Quote: ... ChIP-seq libraries were sequenced on the Illumina NextSeq 500 system with a 20% phiX spike-in (Illumina, FC-110-3001) to generate 75 bp single-end reads (NextSeq 500/550 High Output v2 kit) ...
-
bioRxiv - Immunology 2020Quote: ... The second round PCR (8 cycles, 70°C annealing temperature) was performed using Nextera XT index primers (Illumina, FC-131-2001) which introduce 8 base pair indices on the 5’ and 3’ termini of the amplicon for data demultiplexing of each sample screened ...
-
bioRxiv - Neuroscience 2022Quote: ... Amplified cDNA (0.125-0.375 ng) was used for generating sequencing libraries with the Illumina Nextera XT DNA kit (Illumina, FC-131-1096). All libraries were sequenced as paired-end 75 using the Illumina HiSeq4000 system.
-
bioRxiv - Synthetic Biology 2019Quote: ... The RNAseq data was generated on an Illumina NextSeq500 75 SE high-output flow-cell (Illumina, cat no. FC-404-2005). The run yielded approximately 550 million reads ...
-
bioRxiv - Evolutionary Biology 2019Quote: We assessed input gDNA quantity using Qubit and normalised the samples to 20ng/ul as described in TruSeq®DNA PCR-Free Library Prep Reference Guide (#FC-121-3001, Illumina) prior fragmentation to 350bp with Covaris S2 ...
-
bioRxiv - Molecular Biology 2020Quote: We performed 8 additional cycles of PCR with Nextera 24-Index kit for indexing before sample pooling (Illumina, FC-121-1011), for which we used 7.5 μl of the above elute as template ...
-
bioRxiv - Physiology 2020Quote: ... Samples were sequenced Single-reads 76 bases using the NextSeq 500 High Output Kit 75-cycles (Illumina, Cat# FC-404-1005), and primary data analysis was performed with the Illumina RTA version 2.4.11 and Basecalling Version bcl2fastq-2.20.0.422.
-
bioRxiv - Microbiology 2020Quote: ... aeruginosa (Pae5 and Pae1505) genomic DNA sequencing libraries were prepared using Nextera DNA library prep kit (Illumina, Cat # FC-121-1031) and utilizing the Nextera DNA Sample Preparation Index Kit (Illumina ...
-
bioRxiv - Cell Biology 2020Quote: ... and DNA libraries for sequencing were constructed using Nextera XT DNA Library Prep kit (FC-131-1024, Illumina, San Diego, CA). The 24 prepared libraries were pooled and submitted to Michigan State University Genomics Facility ...
-
bioRxiv - Genomics 2021Quote: ... Libraries were prepared based on a 20X scaled-down Nextera XT DNA Library Prep Kit (Illumina, Cat. No. FC-131-1024) protocol (35 ...
-
bioRxiv - Genomics 2021Quote: ... and 8.8 µl of paired i7 and i5 Nextera XT Index Kit V2 indexing primers (Illumina, Cat. No. FC-131-2001) were added to each Tagmentation reaction ...
-
bioRxiv - Neuroscience 2021Quote: ... Tagmentation by Tn5 was performed using reagents from the Nextera DNA Sample Preparation Kit (FC-121-1030, Illumina; San Diego, CA) as previously described [5] ...
-
bioRxiv - Genomics 2020Quote: ... and sequenced on an Illumina NextSeq 500 (NextSeq control software v2.0.2/Real Time Analysis v2.4.11) using a 150-cycle NextSeq 500/550 High Output Reagent Kit v2 (Illumina, FC-404-2002) in standalone mode as follows ...
-
bioRxiv - Immunology 2021Quote: ... single reads and 7 bases index read on an Illumina HiSeq2000 instrument using the TruSeq SBS Kit HS-v3 (50-cycle, FC-401-3002, Illumina Inc). RNA seq data in FASTQ form from each sample were first quality assessed using fastQC ...
-
bioRxiv - Genomics 2020Quote: ... Nuclei were resuspended in 50 ul of the transposase reaction buffer (Nextera DNA library prep kit (Illumina cat. FC-121- 1030) and 22.5 ul nuclease-free water ...