Labshake search
Citations for Illumina :
451 - 500 of 1045 citations for Siglec 6 Human HEK 293 Fc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... Index i7 (6 pb barcode) was read with primer HP8 (Illumina).
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bioRxiv - Genomics 2020Quote: ... Human libraries were sequenced on a NovaSeq 6000 (Illumina) and mouse libraries on a NextSeq 500 (Illumina).
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bioRxiv - Cancer Biology 2023Quote: ... mRNA expression log intensity levels (Illumina Human v3 microarray) were used as the expression levels of the genes ...
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bioRxiv - Developmental Biology 2021Quote: ... Cells were counted and 50,000 cells were isolated for processing with the Nextera Tn5 enzyme (Illumina FC-131-1096) as previously described (Buenrostro et al. ...
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bioRxiv - Genomics 2020Quote: ... 0.5 μl of each index primers i5 and i7 from Nextera Index Kit (Illumina, catalog no. FC-121-1011) and 1.5 μl H2O ...
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bioRxiv - Genomics 2020Quote: ... Hiseq3000 SBS&Cluster high-throughput NGS library preparation kit (Cat. No. FC-410-1002, Illumina, San Diego, CA, USA), (VAHTS Universal DNA Library Prep Kit (ND608-01 ...
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bioRxiv - Genomics 2020Quote: ... 0.5 μl of each index primers i5 and i7 from Nextera Index Kit (Illumina, catalog no. FC-121-1011) and 1.5 μl H2O ...
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bioRxiv - Genomics 2020Quote: ... Samples that passed quality checks were tagmented with the Nextera DNA Library Prep Kit (Illumina cat# FC-121-1030). Tagmented samples were cleaned using the QIAquick DNA column (Qiagen cat# 28104) ...
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bioRxiv - Immunology 2021Quote: ... and amplicons were used to perform the index PCR using the Nextera XT index v2 (Illumina #FC-131-1002). The index PCR products were cleaned with the Agencourt AMPure XP kit (Beckman Coulter #A63881 ...
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bioRxiv - Developmental Biology 2019Quote: ... magnetic beads were then washed 2x in 1ml 10uM TrisHCl pH=7.4 and finally resuspended in 24 ul of pre-warmed tagmentation buffer (FC-121-1030, Illumina). 1 ul of TDE1 enzyme was added to the mix and the beads were incubated for 120” at 37°C ...
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bioRxiv - Microbiology 2019Quote: ... MS-103-2003) at a concentration of 15 nM with addition 25% Phix Control v3 (Illumina, FC-11-2003). The Mi-Seq separates all sequencing data by sample during post-run processing using recognised barcoded indices to generate FASTAQ files ...
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bioRxiv - Molecular Biology 2019Quote: ... Nuclei were re-suspended in transposase reaction mix (25 μL 2×TD buffer, 2.5 μL transposase (FC-121-1030, Illumina) and 22.5 μL nuclease-free water ...
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bioRxiv - Systems Biology 2019Quote: ... Libraries were loaded with a concentration of 2.2pM on 75 cycle high output flow cells (Illumina, FC-404-2005) and sequenced on a NextSeq 500 (Illumina ...
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bioRxiv - Molecular Biology 2019Quote: ... MS-103-2003) at a concentration of 15 nM with addition 25% Phix Control v3 (Illumina, FC-11-2003). The MiSeq separated all sequences by sample during post-run processing by recognised indices and to generate FASTQ files.
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bioRxiv - Genomics 2019Quote: ... Then perform tagmentation using 2 μl of Tn5 transposase and 12.5 ul 2 × TD buffer (Illumina #FC-121-1031) at 37°C for 1h with 650 rpm shaking ...
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bioRxiv - Genomics 2019Quote: ATAC-seq was carried out as previously described using the Nextera DNA Library Prep Kit (Illumina #FC-121-1030)60 ...
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bioRxiv - Evolutionary Biology 2019Quote: ... MS-103-2003) at a concentration of 15 nM with addition 25% Phix Control v3 (Illumina, FC-11-2003).
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bioRxiv - Microbiology 2019Quote: ... Viral DNA libraries were generated by Nextera XT DNA Library Preparation Kit (Cat. No. FC-131-1096, Illumina, USA) by a slightly modified manufactures protocol divided into” Genomic DNA tagmentation” and “PCR clean-up” ...
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bioRxiv - Neuroscience 2020Quote: ... and sequenced on an Illumina HiSeq 2500 using HiSeq SBS kit v4 250 cycle kit (Illumina, FC-401-4003). A standard Illumina pipeline was used to generate fastq files ...
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bioRxiv - Physiology 2021Quote: ... Libraries were prepared from ∼150 pg of DNA using the Nextera XT DNA preparation kit (Illumina, FC-131-1096) and Nextera XT 96-Index kit (Illumina ...
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bioRxiv - Cell Biology 2021Quote: ... Libraries were loaded with a concentration of 2.2pM on 75 cycle high output flow cells (Illumina, FC-404-2005) and sequenced on a NextSeq 500/550 (Illumina ...
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bioRxiv - Cell Biology 2021Quote: ... and metagenomic DNA sequencing libraries were constructed using the Nextera XT DNA Library Prep Kit (FC-131-1096, Illumina) and sequenced on a NextSeq500 Sequencing System as 2 x 150 nucleotide paired-end reads ...
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bioRxiv - Neuroscience 2019Quote: ... we tagmented 600 pg of DNA using the Nextera XT DNA Sample Preparation Kit (Illumina, cat # FC-131-1096). Libraries were further amplified with 12 PCR cycles using custom P5-TSO hybrid and custom Nextera-compatible primers with different indexes ...
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bioRxiv - Molecular Biology 2020Quote: ... 2.5μL Tn5 transposase and 22.5μL ddH2O) using reagents from the Nextera DNA library Preparation Kit (Illumina #FC-121-103). Samples were then incubated at 37°C for 30min ...
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bioRxiv - Molecular Biology 2022Quote: ... Nuclei pellets were resuspended gently in transposition reaction mix (25 µL 2X TD Buffer [Illumina Cat #FC-121-1030], 2.5 µL transposase [Illumina Cat #FC-121-1030] and 22.5 µL nuclease-free water ...
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bioRxiv - Neuroscience 2021Quote: ... Pooled libraries were sequenced using the NextSeq 500/550 High Output v2 kit (75 cycles, Illumina, FC-404-2005) with single reads on a NextSeq500 (Illumina).
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bioRxiv - Neuroscience 2021Quote: ... nuclei were extracted from cells and treated with transposition mixture containing Nextera Tn5 Transposase for (Illumina, FC-121-1030). Transposed fragments were then purified using QIAGEN MinElute columns (QIAGEN ...
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bioRxiv - Microbiology 2021Quote: ... and libraries were prepared with a Nextera XT DNA kit (catalog number FC-131-1096; Illumina, San Diego, CA). Normalization ...
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bioRxiv - Cancer Biology 2021Quote: ... and sequencing was followed previously reported protocols.67,68 The sequencing library was constructed by using the Nextera XT tagmentation kit (Cat# FC-131-1096, Illumina), and the HiSeq2500 instrument (Illumina ...
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bioRxiv - Cancer Biology 2021Quote: ... Library construction was performed using the Nextera XT Library Prep kit (#FC-131-1024, Illumina, San Diego, California, USA) and custom barcode adapters (sequences available upon request) ...
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bioRxiv - Microbiology 2019Quote: ... Libraries were constructed with the Nextera XT DNA Library Prep Kit (FC-131-1096, Illumina, San Diego, California, USA). All reads were finished using CLC Genomics Workbench v ...
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bioRxiv - Genetics 2019Quote: Sequencing libraries were prepared using the truseq Nano DNA sample preparation kit (T FC-121-4001/4002, Illumina Inc) extracting 100 ng DNA for each sample ...
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bioRxiv - Molecular Biology 2020Quote: ... 120 μl pooled 20 pM library and 15 μl denatured 20 pM PhiX control library (Illumina, FC-110-3001) after a 2 minute heat treatment at 96 °C followed by a 5 min incubation on ice ...
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bioRxiv - Genomics 2021Quote: ... Each sample library was uniquely barcoded and quantified by qPCR using a PhiX Control v3 (Illumina, FC-110-3001) standard curve ...
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bioRxiv - Genomics 2019Quote: ... and sequenced on an Illumina NextSeq 500 instrument with a High Output v2 reagent kit (Illumina #FC-404-2005), reading 86 nt for read 1 and 6 nt for the P7 index read ...
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bioRxiv - Genetics 2019Quote: ... All sequencing followed the protocol in the Illumina TruSeq PCR-Free Sample Preparation Guide (Illumina cat# FC-121-2001), and used PCR-free library preparation kits purchased from KAPA Biosystems (see https://www.nhlbiwgs.org/topmed-whole-genome-sequencing-project-freeze-5b-phases-1-and-2 for additional details) ...
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bioRxiv - Physiology 2020Quote: ... which was processed into the sequencing library using the Nextera XT DNA Library Preparation Kit (FC-131-1096, Illumina) with unique barcode sequences for each set ...
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bioRxiv - Cancer Biology 2021Quote: ... Transposed fragments were amplified and purified as described previously (Buenrostro 2015) with Nextera Index Kit (FC-121-1011, Illumina). qPCR was performed to determine the optimal number of cycles to amplify the library to reduce artifacts associated with saturation PCR of complex libraries ...
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bioRxiv - Genomics 2021Quote: Sequencing libraries were generated using the sequencing kit: TruSeq SBS Kit v5 – GA (36 Cycle) (FC- 104-5001, Illumina). Samples were then sequenced on an Illumina GA-IIx sequencer using paired-end (PE ...
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bioRxiv - Genomics 2021Quote: ... and tagmented using the enzyme and buffer provided in the Nextera Library Prep Kit (Illumina, cat. FC-121-1031). Tagmented DNA was then purified using the MinElute PCR purification kit (Qiagen ...
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bioRxiv - Cancer Biology 2022Quote: ... PCR-free libraries were prepared from 1μg DNA using the TruSeq PCRfree DNA sample preparation kit (cat# FC-121-3001/3002, Illumina) targeting an insert size of 350bp ...
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bioRxiv - Cell Biology 2022Quote: ... 2.5 µl of Tagment DNA buffer 1.25 µl of Amplicon Tagment Mix (Nextera XT kit, Illumina FC-131-1096) were added ...
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bioRxiv - Genomics 2022Quote: ... 600 pg cDNA from each sample plate was used in a modified Nextera XT (Illumina, Cat. FC-131-1024) library preparation but using the P5NEXTPT5 primer and the tagmentation time of 5 mins ...
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bioRxiv - Genetics 2022Quote: ... Normalised DNA libraries were clustered on Illumina cBot then sequenced using Illumina HiSeq X Ten platform using HiSeq X Ten Reagent Kit v2.5 kits (FC-501-2501, Illumina). Paired end sequencing was performed using the 2×150bp chemistry to achieve an average output of approximately >120 Gb of data per library.
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bioRxiv - Cell Biology 2023Quote: ... Samples were diluted to 0.2 ng/ul in TE and tagmented (Nextera XT DNA Library Preparation Kit (#FC-131-1096, Illumina). Indexing was performed using the Nextera XT Index Kit (#FC-131-1001 ...
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bioRxiv - Cell Biology 2022Quote: ... 10μl of the tagmented chromatin was mixed with 2.5μl of Nextera PCR primer cocktail and 7.5μl of Nextera PCR master-mix (Illumina FC-121-1030) in low-binding PCR tubes ...
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bioRxiv - Microbiology 2023Quote: ... Libraries were pooled in equimolar concentrations and sequenced using an Illumina NextSeq 500/550 sequencer (Illumina, FC-404-2005). At least 95% of the reads generated presented a Q score of ≥ 30 ...
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bioRxiv - Microbiology 2024Quote: ... This PCR product was prepared for sequencing using the Nextera XT DNA Sample Prep Kit (Illumina, FC-131-1096) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... Two arrays were sequenced per sequencing run with an Illumina 75 Cycle NextSeq500/550v2 kit (Illumina FC-404–2005) at a final concentration of 2.4 pM ...
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bioRxiv - Cancer Biology 2023Quote: ... Samples were added with 50mL trans-position reaction mix of Nextera DNA library preparation kit (FC-121-1031, Illumina). DNA was amplified by PCR and incubated at 37°C for 30 minutes ...