Labshake search
Citations for Illumina :
451 - 500 of 1549 citations for Glucagon like peptide 1 receptor GLP1R Human HEK293 Fc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... Full-length cDNA was then processed with a Nextera XT DNA Library Preparation Kit (Illumina, FC-131-1096). This kit aims to fragment and add adapter sequences onto template DNA with a single tube Nextera XT tagmentation reaction and so generate multiplex sequencing libraries ...
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bioRxiv - Cancer Biology 2020Quote: ... RNA-sequencing was performed using NextSeq75 High Output v2 kit and NextSeq 500 (Illumina; cat# FC-404-2005). Using TruSeq 3’ SE adaptor sequence AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC ...
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bioRxiv - Microbiology 2022Quote: ... A second PCR step was performed with Illumina Nextera XT Index Kit v2 (Illumina, Cat.:FC-131-2001) and Ex Taq DNA Polymerase (TaKaRa Bio ...
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bioRxiv - Physiology 2022Quote: ... Samples were pooled and sequenced on the Illumina NextSeq 500/550 High Output platform (Illumina, FC-404-2002) up to 18 samples per lane with 1% PhiX spike as a control.
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bioRxiv - Plant Biology 2021Quote: ... the Tn5 reaction was prepared and mixed well with nuclei using the Nextera reagents (Illumina, FC-121-1030) as follows ...
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bioRxiv - Microbiology 2020Quote: ... Libraries were pooled in equimolar concentrations and sequenced using an Illumina NextSeq 500 sequencer (Illumina, FC-404-2005). At least 95% of the reads generated presented a Q score of ≥ 30 ...
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bioRxiv - Neuroscience 2020Quote: ... The full-length cDNA output was processed with Nextera XT DNA library preparation kit (Illumina #FC-131-1024) according to manufacturer’s instructions ...
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bioRxiv - Genetics 2021Quote: ... The nuclei were resuspended in the transposition reaction mix (2x TD Buffer (Illumina Cat #FC-121-1030, Nextera), 2.5⍰μl Tn5 Transposase (Illumina Cat #FC-121-1030 ...
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bioRxiv - Genomics 2019Quote: ... equivalent to the protocol in the Illumina TruSeq PCR-Free Sample Preparation Guide (Illumina cat# FC-121-2001). Center-specific details are available from the TOPMed website82 ...
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bioRxiv - Genomics 2020Quote: ... Libraries were then prepared following the instructions of the TruSeq DNA Sample Preparation Kit (Illumina, #FC-121-2001) and sequenced on the HiSeq 2000 ...
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bioRxiv - Cancer Biology 2019Quote: ... Tagmentation and library preparation was performed using the Nextera XT DNA Library Preparation Kit (Illumina, Cat# FC-131) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2021Quote: ... Each sample library was uniquely barcoded and quantified by qPCR using a PhiX Control (Illumina, FC-110-3001) standard curve ...
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bioRxiv - Genetics 2021Quote: ... DNA libraries for whole genome sequencing were prepared with Nextera DNA Library Prep Kit (FC-121-1031, Illumina). Libraries for RADseq were prepared according to the procedures of Adapterama III74 with few modifications ...
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bioRxiv - Genetics 2021Quote: ... and Illumina libraries were prepared with different indexes using Nextera XT Library Prep Kit (Illumina, FC-131-1096). Prior to sequencing ...
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bioRxiv - Physiology 2021Quote: Sequencing libraries were prepared using the Illumina Nextera XT DNA Sample Preparation kit (Illumina, Ref. FC-131-1096) and the combination of 384 Unique Dual Indexes (Illumina-Set A to D ...
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bioRxiv - Neuroscience 2020Quote: ... Approximately 50.000 nuclei were used for the transposition reaction using hyperactive Tn5 transposase (Illumina Cat #FC-121-1030) followed by 13 cycles of PCR amplification ...
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bioRxiv - Immunology 2020Quote: ... the sequencing library was then created from cDNA using the Illumina Nextera XT method (Illumina FC-131-1096). All libraries were combined and sequenced on Illumina HiSeq4000 lanes ...
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bioRxiv - Developmental Biology 2020Quote: ... Nuclei were then resuspended in transposase reaction mix for 30 min at 37 °C (Illumina, Fc-121-1030). The samples were purified using Qiagen MiniElute kit (QIAGEN ...
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bioRxiv - Genomics 2020Quote: ... we used 0.2ng of cDNA per cell and one-eighth of the Illumina NexteraXT (Illumina FC-131-1096) reaction volume ...
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bioRxiv - Developmental Biology 2022Quote: ... nuclei were extracted from cells and treated with transposition mixture containing Nextera Tn5 Transposase (Illumina, FC-121-1030) for 30 minutes at 37°C with 1000 RPM mixing ...
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bioRxiv - Cancer Biology 2022Quote: ... before being processed for Illumina sequencing via the Nextera XT DNA Library Preparation kit (Illumina; FC-131-1096). Sequencing was performed on an Illumina platform.
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bioRxiv - Genomics 2022Quote: ... Nuclei pellets were resuspended in 50 μL transposition reaction containing 2.5 μL Tn5 transposase (FC-121-1030; Illumina). The reaction was incubated in a 37°C heat block for 45 min ...
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bioRxiv - Neuroscience 2022Quote: Sequencing libraries were prepared using the Illumina Nextera XT DNA Sample Preparation kit (Illumina, Ref. FC-131-1096) and the combination of 384 Combinatorial Dual Indexes (Illumina-Set A to D ...
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bioRxiv - Microbiology 2024Quote: ... DNA for Illumina sequencing was prepared using the Nextera XT DNA library prep kit (Illumina FC-131-1096) with the Nextera XT v2 Index Kit B (Illumina FC-131-2002 ...
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bioRxiv - Genetics 2024Quote: ... The 0.2 M NaOH was used to denature the pooled library and PhiX library (Illumina, FC-110-3001). After diluting the libraries with pre-chilled HT1 to 12 pM ...
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bioRxiv - Microbiology 2023Quote: Adapter ligation and barcoding were performed using the Nextera XT Index Kit (#FC-131-1002, Illumina®, UK). PCR conditions involved 3 min of denaturation at 98°C ...
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bioRxiv - Synthetic Biology 2023Quote: The sequencing libraries were mixed with 20–30% of PhiX spike-in DNA control (Illumina #FC-110-3001) for better cluster generation on the flow cell and sequenced by Illumina MiSeq (MiSeq v3 150-cycles kit #MS-102-3001 or 300-cycles kit #MS-102-3003) ...
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bioRxiv - Molecular Biology 2023Quote: ... Pooled amplicons were then sequenced on an Illumina MiniSeq system (300 cycles, Illumina sequencing kit #FC-420-1004) following the manufacturer’s protocol ...
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bioRxiv - Genomics 2023Quote: Next-generation sequencing libraries were prepared using the Nextera XT DNA Library Preparation Kit (FC-131-1096, Illumina). Following fluorometric quantification ...
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Planarians employ diverse and dynamic stem cell microenvironments to support whole-body regenerationbioRxiv - Developmental Biology 2023Quote: Subsequent library preparation was performed according to manufacturer’s directions for the Nextera XT kit (Illumina, FC-131-1096) starting with 600 pg of cDNA and using a specific P5-Truseq PCR hybrid oligo in place of the Nextera XT i5 adapter (15ul Nextera PCR mix ...
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bioRxiv - Genomics 2022Quote: ... 2.5 μL each of Nextera i5 and i7 indexed amplification primers (Nextera Index Kit, Illumina, FC-121—1011) and 10 μl of nuclease free H2O ...
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bioRxiv - Genomics 2022Quote: ... To the 1.5 μl concentrated air DNA extraction we added 2.5 μl Illumina Nextera reaction buffer (FC-121-1030, Illumina), 1 μl 1 pg/μl Lambda DNA (SD0011 ...
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bioRxiv - Genomics 2023Quote: ... Sequencing libraries for ATAC samples were prepared using the Nextera DNA library preparation kit (Illumina, #FC-121-1030) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2023Quote: ... 12.5 μl of a master mix containing 7.5 μl of Nextera PCR Master Mix (Illumina, FC-131-1096) and 2.5μl of each Index primer i7 (Illumina ...
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bioRxiv - Systems Biology 2023Quote: Sequencing libraries were prepared by tagmenting 10 ng WTA DNA with Nextera XT kit (Illumina #FC-131-1024). Indexing PCR was primed with a nextera i7 index primer (e.g ...
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bioRxiv - Genomics 2023Quote: ... Pooled male and pooled female libraries were constructed using a Truseq nano kit (Illumina, ref. FC-121-4001) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... The amplified fragments were sequenced using a 150-cycle MiniSeq High Output Reagent Kit (Illumina, FC-420-1002) on an Illumina MiniSeq ...
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bioRxiv - Microbiology 2023Quote: ... The amplified barcodes were sequenced using a 75-cycle MiniSeq High Output Reagent Kit (Illumina, FC-420-1001) on an Illumina MiniSeq at Michigan State University ...
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ATP-dependent citrate lyase Drives Left Ventricular Dysfunction by Metabolic Remodeling of the HeartbioRxiv - Molecular Biology 2024Quote: ... using a HiSeq 3000/4000 SBS 300-cycle kit (Cat#FC-410-1003, Illumina, Inc., San Diego, CA) for 2×150 paired-end reads ...
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bioRxiv - Synthetic Biology 2024Quote: ... The whole plasmid sequences were verified using Nextera XT DNA library prep kit (Illumina, Catalog #FC-131-1096) or whole plasmid sequencing services provided by Plasmidsaurus Inc ...
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bioRxiv - Developmental Biology 2021Quote: ... Cells were counted and 50,000 cells were isolated for processing with the Nextera Tn5 enzyme (Illumina FC-131-1096) as previously described (Buenrostro et al. ...
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bioRxiv - Genomics 2020Quote: ... 0.5 μl of each index primers i5 and i7 from Nextera Index Kit (Illumina, catalog no. FC-121-1011) and 1.5 μl H2O ...
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bioRxiv - Genomics 2020Quote: ... Hiseq3000 SBS&Cluster high-throughput NGS library preparation kit (Cat. No. FC-410-1002, Illumina, San Diego, CA, USA), (VAHTS Universal DNA Library Prep Kit (ND608-01 ...
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bioRxiv - Genomics 2020Quote: ... 0.5 μl of each index primers i5 and i7 from Nextera Index Kit (Illumina, catalog no. FC-121-1011) and 1.5 μl H2O ...
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bioRxiv - Genomics 2020Quote: ... Samples that passed quality checks were tagmented with the Nextera DNA Library Prep Kit (Illumina cat# FC-121-1030). Tagmented samples were cleaned using the QIAquick DNA column (Qiagen cat# 28104) ...
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bioRxiv - Immunology 2021Quote: ... and amplicons were used to perform the index PCR using the Nextera XT index v2 (Illumina #FC-131-1002). The index PCR products were cleaned with the Agencourt AMPure XP kit (Beckman Coulter #A63881 ...
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bioRxiv - Developmental Biology 2019Quote: ... magnetic beads were then washed 2x in 1ml 10uM TrisHCl pH=7.4 and finally resuspended in 24 ul of pre-warmed tagmentation buffer (FC-121-1030, Illumina). 1 ul of TDE1 enzyme was added to the mix and the beads were incubated for 120” at 37°C ...
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bioRxiv - Microbiology 2019Quote: ... MS-103-2003) at a concentration of 15 nM with addition 25% Phix Control v3 (Illumina, FC-11-2003). The Mi-Seq separates all sequencing data by sample during post-run processing using recognised barcoded indices to generate FASTAQ files ...
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bioRxiv - Molecular Biology 2019Quote: ... Nuclei were re-suspended in transposase reaction mix (25 μL 2×TD buffer, 2.5 μL transposase (FC-121-1030, Illumina) and 22.5 μL nuclease-free water ...
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bioRxiv - Systems Biology 2019Quote: ... Libraries were loaded with a concentration of 2.2pM on 75 cycle high output flow cells (Illumina, FC-404-2005) and sequenced on a NextSeq 500 (Illumina ...