Labshake search
Citations for Illumina :
451 - 500 of 650 citations for 1 Methoxy 4 1 methylbutyl benzene since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... Pooled libraries of 1 nM were denatured and diluted as per the Standard Normalization Method for the NextSeq® System (Illumina). Final loading concentration of 1.7 pM and 1% (v/v ...
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bioRxiv - Immunology 2020Quote: ... Final loading concentration of 1.7 pM and 1% (v/v) PhiX was used with the NextSeq® 500/550 High Output Kit v2 (150 cycles) (Illumina Samples were sequenced paired-end with 75 cycles of each read and single index of 6 cycles.
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bioRxiv - Immunology 2021Quote: ... whose lengths were about 600 base pairs were sequenced using an Illumina Novaseq 6000 S4 flowcell (67 bp read 1 and 140 bp read 2) (Illumina, USA). Only read2 contained the sequence regarding the definition of T cell clones.
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bioRxiv - Genetics 2023Quote: ... was used to isolate total RNA and approximately 1 ug of RNA was processed with the TruSeq Stranded mRNA Library Prep Kit (Illumina 20020594) to prepare libraries for paired-end sequencing on Nova-seq 6000 ...
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bioRxiv - Neuroscience 2022Quote: ... Single-end sequencing (i.e, 1×75bp) was performed in a single run on an Illumina NextSeq 500 (Illumina, San Diego, CA). Three independent experimental replicates were run for the Cebpg shRNA-mediated knockdown and shRNA control transgenic lines for a total of 6 libraries.
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bioRxiv - Developmental Biology 2023Quote: ... PolyA+ RNA-seq libraries were produced from 1 µg of RNA using the TruSeq® Stranded mRNA Library Prep (Illumina, 20020594) kit ...
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Dysregulated expanded endocannabinoid system as therapeutic targets of amyotrophic lateral sclerosisbioRxiv - Neuroscience 2024Quote: ... A library was independently prepared with 1 µg of total RNA for each sample by an Illumina TruSeq Stranded mRNA Sample Prep Kit (Illumina, Inc.). The first step in the workflow involved purifying the poly-A-containing mRNA molecules using poly-T-attached magnetic beads ...
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bioRxiv - Plant Biology 2024Quote: ... total RNA from Col-0 and hakai-1 (Mock and Hpa) three biological replicates (same samples used for Illumina RNA-seq) were pooled into a single sample ...
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bioRxiv - Microbiology 2024Quote: Libraries for Illumina short read sequencing were prepared from 1 ng of extracted DNA utilizing the Nextera XT DNA Library Prep Kit according to the manufacturer’s recommendations (Illumina Inc., USA). Sequencing was carried out in paired-end (2×300 base pairs ...
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bioRxiv - Molecular Biology 2022Quote: ... PolyA + fraction (1 ng for each sample) was used for whole-transcriptome library preparation using ScriptSeq v2 RNA-Seq kit (Illumina, USA). Libraries amplified in 14 PCR cycles were purified using Agencourt AMPure XP beads (Beckman-Coulter ...
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bioRxiv - Cancer Biology 2023Quote: ... RNAseq libraries were prepared from total RNA with the QuantSeq 3′ mRNA-Seq Library Prep Kit FWD for Illumina (Lexogen, 015.24) in combination with the UMI Second Strand Synthesis Module for QuantSeq FWD (Illumina, Read 1) (Lexogen ...
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bioRxiv - Developmental Biology 2024Quote: ... at 1/8th reaction volumes (except for Cas13d control and bckdk mRNA KD samples where 1/4th of the reaction volume were used) paired with IDT for Illumina DNA/RNA UD Indexes Set A (Illumina, 20027213), and purified using the Ampure XP bead-based reagent (Beckman Coulter ...
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bioRxiv - Genetics 2024Quote: ... Amplicon sequencing libraries were prepared as previously described54,70,71 and sequenced with a 1 x 75 single end read on a NextSeq instrument (Illumina, San Diego CA). Individual gRNA reads were extracted from FASTQ files with PoolQ and analyzed for enrichment using MAGeCK72 ...
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bioRxiv - Genomics 2024Quote: Both read 1 and read 2 files from each spatial region were mapped to the mm10 reference genome (source: Illumina igenomes) using the bowtie2 package (v2.5.1 ...
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bioRxiv - Molecular Biology 2024Quote: ... the pool of plasmids for each library (inputs) was amplified using primers bearing sequences from Illumina (Read 1 and Read 2) and complementary to PT1 and down to Moe (Figure 1C) ...
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bioRxiv - Genomics 2024Quote: ... where n is the number of reads covering each site and p is the NGS error rate (that we fixed to 1% as observed for the Illumina platform).
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bioRxiv - Synthetic Biology 2024Quote: ... Barcoded RNA-seq libraries were prepared from 1 μg of total RNA using the TruSeq Stranded mRNA Library Prep kit according to the manufacturer’s instructions (Illumina Inc., USA). RNA-seq libraries were sequenced on an Illumina NovaSeq 6000 System according to the manufacturer’s protocol ...
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bioRxiv - Genomics 2024Quote: We then pooled the original and targeted libraries at a 2:1 molar concentration and sequenced them together on a NovaSeq 6000 (Illumina, 20012850) with a S1 Reagent Kit v1.5 (200 cycles ...
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bioRxiv - Genetics 2024Quote: ... 1 µg of total RNA was used with the TruSeq RNA Sample Prep Kit v.2 (Illumina, San Diego, CA, USA). The cDNA libraries were quantified by quantitative Real-Time PCR (qPCR) ...
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bioRxiv - Cancer Biology 2024Quote: ... Libraries were prepared from 1 ng amplicon DNA by using the Nextera XT library prep kit (Illumina, San Diego, CA, USA) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2021Quote: ... Total RNA (1 μg) from each sample was used for NEBNext PolyA selection and Ultra Directional RNA Library preparation (Illumina, E7490, E7420). Prepared libraries were sequenced using 2×150 bp paired-end read chemistry on an Illumina HiSeq 4000 platform.
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bioRxiv - Molecular Biology 2021Quote: ... 1 ug of treated RNA was then input into the Illumina TruSeq Small RNA Library Preparation Kit (Illumina; Cat #: RS-200-0012) with 11 cycles of PCR amplification ...
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bioRxiv - Genomics 2020Quote: ... Samples were prepared for sequencing using 1 ug of genomic DNA following the TruSeq PCR free kit protocol (Illumina, FC-121-3001). Resulting libraries were quality checked on an Agilent DNA 1000 bioanalyzer (Agilent Technologies ...
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bioRxiv - Plant Biology 2020Quote: 42 RNA samples from experiment 1 were used for mRNA-Seq library preparation using the TruSeq Stranded mRNA Library Prep Kit (Illumina, CA, USA). Libraries were multiplexed and single-end 100 nt RNA-Seq was performed on a Novaseq 6000 at the GIGA Center (University of Liege ...
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bioRxiv - Genomics 2022Quote: ... a library was prepared from 1 μg of genomic DNA and a TruSeq DNA PCR-free Sample Preparation Kit (Illumina, CA, USA). Paired-end (151 bp per read ...
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bioRxiv - Genomics 2022Quote: ... Libraries were prepared with 1 μg of total RNA for each sample using a TruSeq Stranded mRNA Sample Prep Kit (Illumina, CA, USA). Paired-end sequencing (151 bp ...
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bioRxiv - Microbiology 2022Quote: ... The V4 region of the 16S rRNA gene was amplified from 1 or 7μl DNA and sequenced with a MiSeq (Illumina, San Diego, CA) using the 500 cycle MiSeq Reagent Kit ...
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bioRxiv - Cell Biology 2020Quote: ... cDNA was diluted 1:15 and subsequently tagmented and amplified for 12 cycles using the Nextera XT DNA Library Preparation Kit (Illumina FC-131). Individual libraries were QC’d by Qubit and Agilent Bioanalyzer ...
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bioRxiv - Genetics 2020Quote: ... One sequencing experiment (Experiment 1) was performed according to the manufacturer’s directions for the TruSeq Small RNA Sample Preparation Kit (Illumina, RS-200-0012). The protocol was adapted to incorporate a 2S blocking DNA oligo for removal of prevalent Drosophila small ribosomal RNA from the sequencing library [67] ...
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bioRxiv - Genomics 2020Quote: ... The cDNA library was generated from 1 µg of total RNA using TruSeq RNA Sample Prep Kit (Illumina, San Diego, CA, USA) following manufacturer’s protocols ...
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bioRxiv - Immunology 2020Quote: ... The Illumina RNA-seq cDNA library was prepared using 1 μg of RNA from each sample and TruSeq V2 kits (Illumina, CA, USA), with reduced RNA fragmentation time (3 minutes ...
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bioRxiv - Plant Biology 2021Quote: ... Libraries for RNA-Seq were prepared from 1 µg of total RNAs with the TruSeq Stranded mRNA Library Prep Kit (Illumina, CA, USA), multiplexed and sequenced in two runs with an Illumina NextSeq500 device (high throughput mode ...
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bioRxiv - Systems Biology 2023Quote: ... 0.5 µL of universal primer and 0.5 µL of index primer (each at 0.5µM concentration; NEBNext multiplex oligos for Illumina index primers set 1 E7335). This mix has been amplified for 15 cycles (same amplification conditions as before) ...
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bioRxiv - Genomics 2023Quote: 1 ng of genomic DNA from each sample was used with Illumina MiSeq Nextera XT DNA Library Preparation Kit (Illumina, Inc., USA), the paired-end library was constructed with an insert (500 bp ...
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bioRxiv - Immunology 2023Quote: ... and sequencing was performed in a 1×100 bp single-read format using a NovaSeq 6000 v1.5 100-cycle reagent kit (Illumina, Cat no. 20028316). Base calling was performed with Illumina Real Time Analysis (RTA ...
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bioRxiv - Genomics 2023Quote: ... Libraries were prepared using the QuantSeq 3’ mRNA-Seq Library Prep Kit FWD for Illumina and UMI Second Strand Synthesis Module for QuantSeq FWD (Illumina, Read 1) from Lexogen (015.96 and 081.96 ...
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bioRxiv - Evolutionary Biology 2023Quote: ... and g2997a) at the forward primer binding site used in the ‘round 1 PCR’ step below (the step attaching partial Illumina sequencing adaptors), thus excluding PCR amplification of the competitor viruses during sequencing library preparation such that only the library viruses ...
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bioRxiv - Molecular Biology 2022Quote: ... 1 µg of total RNA was used as input for the TruSeq RNA Library Prep Kit v2 (Illumina, Inc., San Diego, CA) and the manufacturer’s protocol was followed ...
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bioRxiv - Neuroscience 2024Quote: ... Illumina Sequencing libraries were prepared using the TruSeq low sample protocol from 1 µg of total RNA (Illumina, San Diego, CA, USA). The resulting libraries were sequenced in paired-end mode ...
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bioRxiv - Genomics 2024Quote: DNA concentration was normalized to 1 ng/μl and subjected to library preparation using Nextera XT DNA Library Preparation Kit (Illumina, CA, USA) according to the manufacturer’s instructions and sequenced with NextSeq550 using Mid Output Kit v2.5 (300 cycles ...
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bioRxiv - Genetics 2023Quote: ... 10 ng of amplicon DNA from PCR reaction 1 was combined with 10 µL of Nextera adapter index primers (Illumina Cat#20027213) and 25 µL Kapa HiFi Hotstart ReadyMix ...
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bioRxiv - Neuroscience 2023Quote: ... Female and male samples after MR1 were sequenced (single-end reads, 1×75 nt) with Illumina NextSeq 500 (Illumina, San Diego, USA). Female samples after MR2 were sequenced (single-end reads ...
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bioRxiv - Immunology 2022Quote: ... Amplified (1 ng) cDNA was used to generate barcoded libraries using the Nextera XT DNA library preparation kit (Illumina, San Diego, CA). The size of the libraries was checked using the Agilent 2100 Bioanalyzer High Sensitivity DNA chip and their concentration was determined using the Qubit® fluorometer (ThermoFisher Scientific ...
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bioRxiv - Genomics 2022Quote: ... we generated a combination of noisy ONT sequencing (MinION R.9.4.1 flow cells with the LSK-109 ligation library prep kit) and Illumina sequencing (PE 150 bp, ∼49x coverage; Illumina NovaSeq 6000). Based on published ONT benchmarking (https://nanoporetech.com/accuracy) ...
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Lysyl oxidase regulates epithelial differentiation and barrier integrity in eosinophilic esophagitisbioRxiv - Cell Biology 2023Quote: ... Sequencing libraries were constructed from total RNA (1 μg) using a TruSeq Stranded mRNA Library Prep (Illumina Inc., San Diego, CA, USA). RNA sequencing was performed on Illumina HiSeq2000 platform ...
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bioRxiv - Genomics 2024Quote: ... Total RNA-seq libraries were generated from 1 μg of DNase-treated RNA using the TruSeq Stranded total RNA library preparation kit (Illumina, Cat# 15031048) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... Sequencing libraries for Δ2 and Δ3 lines were prepared with 1 μg of gDNA per sample using the TruSeq® DNA PCR-Free Low-Throughput Library Prep Kit (Illumina, 20015962) according to the manufacturer’s directions ...
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bioRxiv - Genomics 2024Quote: ... and 1 μL of TAE polymerase (TruePrep® DNA Library Prep Kit V2 for Illumina®, Cat. no. TD501-02, Vazyme Biotech). PCR thermocycling was carried out in Biometra Tone 96G thermocyclers (Analytik Jena) ...
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bioRxiv - Immunology 2024Quote: ... cDNA was diluted to an average of 200 pg/µl and 100 pg cDNA from each cell was tagmented by adding 1 µl TD and 0.5 µl ATM from a Nextera XT DNA Library Preparation Kit (Illumina: FC-131-1096) to 0.5 µl diluted cDNA in each well of a fresh 384-well plate ...
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bioRxiv - Systems Biology 2024Quote: ... All libraries were dual-indexed with 8 nucleotide indexes using NEBNext® Multiplex Oligos for Illumina® (Index Primers Set 1) and were sequenced on NextSeq 2000 (Illumina) either by 2×150bp or 2×50bp configuration.