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Citations for Protean :
151 - 200 of 217 citations for 7 N Fmoc aminocoumarin 4 acetic acid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... Blue Native PAGE gel experiments were performed in a cold room (4 °C) using 10% precast gels (BioRad Mini-PROTEAN) and run at 170 V for 55 minutes ...
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bioRxiv - Physiology 2023Quote: ... Total protein (5∼15 µg) was loaded and separated by 4–20% pre-made SDS-PAGE gels (#4568095, BioRad, Mini-PROTEAN TGX Stain-Free Precast Gels ...
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bioRxiv - Microbiology 2023Quote: ... Denatured samples (15 µL) were separated by SDS-PAGE using pre-cast 4 - 20% gels (Mini.PROTEAN TGX Gels, Bio-Rad) and transferred onto a 0.45 µm PVDF membrane (Thermo Scientific™) ...
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bioRxiv - Cancer Biology 2023Quote: ... Ten micrograms of total protein extract underwent electrophoresis in stain-free 4–15% gradient Tris-glycine SDS-polyacrylamide gels (Mini PROTEAN® TGX Stain-Free™ Gels ...
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bioRxiv - Cancer Biology 2023Quote: ... 15 micrograms of total protein were separated using stain-free 4–15% gradient Tris-glycine SDS-polyacrylamide gels (Mini PROTEAN® TGX Stain-Free™ Gels ...
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bioRxiv - Microbiology 2024Quote: ... and the cleared lysate was loaded onto 4-20% Tris-glycine gels (MINI-Protean TGX Pre-cast, Bio-Rad, 4561095) and electrophoresed at 200 V for 30 min ...
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bioRxiv - Microbiology 2024Quote: ... 10 μg of total protein for both NT and Rab siRNA-transfected samples were resolved in 4-20% gradient SDS-PAGE (Mini-PROTEAN Gel ...
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bioRxiv - Molecular Biology 2021Quote: ... 25 μg of total protein extracts were run on a gradient (15-4%) Polyacrylamide gel (MINIi-PROTEAN TGX Precast Gels, Biorad). Proteins were transferred on nitro-cellulose membrane and checked with Red Ponceau staining for efficient transfer ...
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bioRxiv - Biochemistry 2020Quote: ... 20 μl of the protein samples were separated on 4-20% SDS-PAGE (Mini-PROTEAN TGX Precast Protein Gels, Bio-Rad) at 20 mA and 250 V as the limiting voltage.
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bioRxiv - Microbiology 2021Quote: ... Protein concentration was then checked via SDS-PAGE (Bio-Rad 4–20 % Mini-PROTEAN® TGX Stain-Free™ protein gel) and InstantBlue protein stain (Expedeon ...
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bioRxiv - Cell Biology 2022Quote: ... 25µg of protein was loaded per lane into an SDS-PAGE gel (Bio-Rad Mini-Protean pre-cast gels 4-20%) and gels were run at 160V for approximately 90 minutes ...
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bioRxiv - Developmental Biology 2021Quote: Proteins extracted from Padi4CTL and Padi4CKO were subjected to SDS–PAGE (4–20% Mini-PROTEAN® TGX™ Precast gel, Biorad) and then transferred onto a nitrocellulose membrane ...
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bioRxiv - Biophysics 2021Quote: ... Electrophoresis was performed at 200 V for 30 min on a 4–20% polyacrylamide gel (Mini-PROTEAN® TGX™, BioRad) in SDS running buffer (25 mM Tris ...
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bioRxiv - Molecular Biology 2022Quote: ... Protein concentration was checked afterwards via SDS-PAGE (Bio-Rad 4–20% Mini-PROTEAN® TGX Stain-Free™ protein gel) and InstantBlue protein stain (Expedeon ...
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bioRxiv - Biochemistry 2023Quote: ... The total volume of samples was applied to precast gel (Mini-Protean TGX Gels, 4-15% gel, Bio-Rad, CA, USA). SDS-PAGE was carried out following manufacturer instructions ...
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bioRxiv - Microbiology 2022Quote: ... 20 μg of proteins of each sample or both input and eluates from the IPs were heated in Laemmli loading buffer at 95°C for 5 min and loaded on a SDS-polyacrylamide electrophoresis gel (Bio-Rad 4–20% Mini-PROTEAN® TGX Stain-Free™ Protein Gels ...
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bioRxiv - Molecular Biology 2023Quote: ... 20 µl of lysate was separated on a 4-20% resolving gel (Bio-Rad Mini-PROTEAN TGX Precast Gel #456-1094), and transferred to a PVDF membrane for analysis using α-FLAG antibody (Sigma Aldrich #F3165 ...
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Osh6 requires Ist2 for localization to the ER-PM contacts and efficient phosphatidylserine transportbioRxiv - Cell Biology 2020Quote: ... 1.5 μl for bound franction for GFP detection) were loaded on SDS–PAGE (4-20% Mini-PROTEAN TGX Stain-Free, Bio-Rad) and analyzed by Western blot.
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bioRxiv - Physiology 2022Quote: Boiled samples of left ventricular free wall (20 μg) were separated (4 % stacking gel) and resolved via 7.5 % sodium dodecyl sulfate-polyacrylamide gel electrophoresis (Mini-PROTEAN SDS-PAGE, BioRad). Samples were loaded alongside 10 µL protein ladder (Precision Plus Protein Standards Kaleidoscope ladder ...
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bioRxiv - Biochemistry 2021Quote: ... 10 μg of total protein was run on a 4–15% SDS-PAGE gel (Mini-PROTEAN TGX Precast Protein Gel; Bio-Rad). Proteins were then transferred onto a PVDF membrane using a Trans-Blot® SD Semi-Dry Transfer Cell (Bio-Rad ...
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bioRxiv - Biophysics 2020Quote: ... Inputs (2 μl) and elutions (10 μl) samples were analyzed by 4-15% SDS-PAGE (MINI-PROTEAN TGX stain-free, Bio-Rad) and staining with Quick Coomassie (Generon ...
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bioRxiv - Microbiology 2020Quote: ... and boiled for 5 min prior to loading on 4-20% SDS-PAGE gradient gels (Mini-PROTEAN TGX precast gels; Bio-Rad). Precision Plus Protein Unstained Protein Standards (Bio-Rad ...
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bioRxiv - Cell Biology 2020Quote: ... and denatured for 5 min at 95°C in reducing Laemmli buffer before SDS-PAGE analysis under denaturing conditions (4–20 % Mini-PROTEAN TGX Gels). The proteins were then transferred to nitrocellulose membranes (Bio-Rad Laboratories ...
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bioRxiv - Cell Biology 2021Quote: ... Purified septin complexes were analyzed by SDS-PAGE using 4-20% precast polyacrylamide gels (Mini-PROTEAN TGX Gels from Bio-Rad, 4561095), or hand-casted 12% or 15% Tris-glycine polyacrylamide gels ...
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bioRxiv - Cell Biology 2021Quote: ... 10 µg of mESC lysate were separated on 4–12% or 10% SDS-PAGE gels (pre-cast NuPage® GE healthcare or Mini-Protean TGX Stain free precast gels ...
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bioRxiv - Cancer Biology 2022Quote: ... Equal amounts of proteins (30-60µg) were separated via SDS polyacrylamide gel electrophoresis (SDS-PAGE) using precast 4-20% gradient polyacrylamide gels (Mini-Protean TGX, Bio-Rad). Proteins were transferred onto polyvinylidene fluoride (PVDF ...
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bioRxiv - Biophysics 2020Quote: ... supplemented with DTT (0.1 M endconcentration) and then subjected to SDS-PAGE on 12% or 4–20% gradient gels (mini-PROTEAN TGX; Bio-Rad). Proteins were transferred to PVDF membranes (TransBlot® TurboTM LF PVDF ...
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bioRxiv - Microbiology 2021Quote: Lysates in the standard SDS sample buffer were heated and then resolved on a 4–20% SDS/PAGE gradient gel (Mini-PROTEAN, Bio-Rad), followed by electroblotting on a PVDF membrane via a Trans Blot Turbo (Bio-Rad ...
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bioRxiv - Cell Biology 2022Quote: ... and denatured for 10 min at 95°C in reducing Laemmli buffer before SDS-PAGE analysis under denaturing conditions (4–20 % Mini-PROTEAN TGX Gels). The proteins were then transferred to nitrocellulose membranes (Bio-Rad Laboratories ...
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bioRxiv - Microbiology 2022Quote: ... The proteins in the lysates were separated by SDS-polyacrylamide gel electrophoresis (SDS-PAGE) electrophoresis using gradient (4–20 %) PAGE gels (Bio-Rad, Mini-PROTEAN TGX), with a molecular mass marker (Bio-Rad ...
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bioRxiv - Molecular Biology 2023Quote: ... 100 μg of cell lysates were separated on a 4-20% resolving gel (Bio-Rad Mini-PROTEAN TGX Precast Gel #456-1094), transferred to a PVDF membrane for analysis using anti-FLAG (Sigma Aldrich #F3165 ...
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bioRxiv - Microbiology 2020Quote: ... Cellular lysates were separated by SDS-PAGE using 15-20 µg total cellular protein per lane (4-20% Mini-PROTEAN TGX gels (BioRad), 120 V for 1 hour) ...
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bioRxiv - Biochemistry 2020Quote: Cross-linked samples of purified ROS-GC1 fragment (aa 814-1110) were analyzed by SDS-PAGE using gradient gels (4-20%, Mini-PROTEAN TGX gels, Biorad). For in-gel digestion ...
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bioRxiv - Biochemistry 2022Quote: ... The separation and distribution of proteins was confirmed by SDS-PAGE gel (4–20% Mini-PROTEAN® TGX™ Precast Protein Gels, Biorad). Fractions containing high molecular weight species were pooled together and concentrated to <500 µL using a 100-kDa cutoff concentrator ...
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bioRxiv - Bioengineering 2022Quote: ... Approximately 20 μL of the denatured phage was loaded in a 4-20 % gradient gel (Mini-PROTEAN® TGX Stain-Free gel, Biorad) and separated by electrophoresis at 120 V ...
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bioRxiv - Cell Biology 2019Quote: ... Protein samples were heated at 95°C for 5 minutes and 10µL were loaded on SDS-PAGE gels (4-20%Mini-PROTEAN TGX Stain-Free, BioRad). After electrophoresis ...
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bioRxiv - Immunology 2022Quote: ... alum pellets were resuspended in sample loading buffer and boiled at 95 °C for ten minutes before being applied to a precast polyacrylamide gel (4–20% Mini-PROTEAN®, Bio-Rad). Proteins were then transferred to a nitrocellulose membrane using the Trans-Blot Turbo transfer system (Bio-Rad) ...
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bioRxiv - Plant Biology 2022Quote: ... Proteins immunoprecipitated with anti-FLAG antibody were separated by SDS-PAGE (4-15% Mini-PROTEAN®TGX™ Precast Protein Gels (Bio-RAD)) ...
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bioRxiv - Plant Biology 2024Quote: ... a 20 μL mixture of 14N- and 15N-labeled samples was loaded onto a Tris-glycine 4-20% polyacrylamide gel (Mini-PROTEAN TGX; Bio-Rad) and the proteins of the sample separated by electrophoresis at 60 V for 1 h ...
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bioRxiv - Biochemistry 2024Quote: Samples were incubated at 95°C for 10 minutes and loaded onto SDS-polyacrylamide gels (Mini-Protean TGX gels 4-20%, Bio-Rad). Following gel electrophoresis ...
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bioRxiv - Plant Biology 2023Quote: ... Protein extractions containing 5 µg of Chl with 1 x SDS loading buffer were boiled at 100℃ for 5 min and loaded onto a 4 - 20% polyacrylamide gel (Mini Protean TGX, Biorad Laboratories). Proteins were transferred to a PVDF-FL membrane on a Biorad semidry blotting system ...
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bioRxiv - Neuroscience 2021Quote: ... 10 μg of protein per sample were loaded and separated by SDS-PAGE on 4-15 % gradient stain-free tris-glycine gels (Mini Protean TGX, BioRad, Hercules, CA), transferred to low fluorescence PVDF membranes (45 μm ...
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bioRxiv - Bioengineering 2020Quote: ... 1 µg of each protein sample was separated in one dimension using 4-20% tris-glycine gels (Mini-PROTEAN TGX, Bio-Rad Laboratories, Inc.). A 1:6 dilution of the PageRuler Plus pre-stained protein ladder (10 µl ...
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bioRxiv - Neuroscience 2020Quote: ... 10 μg of protein per sample were loaded and separated using SDS-PAGE on 4-15% gradient stain-free tris-glycine gels (Mini Protean TGX, BioRad, Hercules, CA), transferred to low fluorescence PVDF membranes (45 μm ...
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bioRxiv - Biochemistry 2021Quote: ... The samples were then subjected to sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE, Bio-Rad Mini-PROTEAN® TGX™ 4-15% Gel) according to manufacturer’s protocols for electrophoresis at 110 V for 70 min ...
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bioRxiv - Neuroscience 2020Quote: ... 10 μg of protein per sample were loaded and separated by SDS-PAGE on 4-15 % gradient stain-free tris-glycine gels (Mini Protean TGX, BioRad, Hercules, CA), transferred to low fluorescence PVDF membranes (45 μm ...
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bioRxiv - Microbiology 2024Quote: ... DNA substrates used were produced by hybridization of different oligonucleotides (TableSup1) and results of the assay were analyzed on SDSPAGE (Mini-PROTEAN TGX gels – 4-20%).
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Osh6 requires Ist2 for localization to the ER-PM contacts and efficient phosphatidylserine transportbioRxiv - Cell Biology 2020Quote: ... Protein samples were heated at 55°C for 10 min and 10 μl was loaded on SDS–PAGE (4-20% Mini-PROTEAN TGX Stain-Free, Bio-Rad) and analyzed by Western blot.
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bioRxiv - Immunology 2021Quote: ... with 0.5 μl of b-mercaptoethanol per well and heated at 70°C for 10 minutes before separation on a polyacrylamide gel (Bio-Rad Mini-PROTEAN TGX Gel 4-15%) and transferred to a PVDF membrane ...
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bioRxiv - Biochemistry 2021Quote: ... Eluted proteins were analysed by 4—15% sodium dodecyl sulfate polyacrylamide (SDS-PAGE) gel electrophoresis (Mini-PROTEAN® TGX™ Precast Gels by Bio-rad) using broad range molecular weight markers (PageRuler™ Plus Prestained Protein Ladder ...