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Citations for Protean :
251 - 300 of 400 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... An equal amount of protein from control and each experiment was loaded onto 4-20% SDS-PAGE gels (Mini-PROTEAN TGX Gels, Bio-Rad, USA) and transferred to the nitrocellulose membranes (Amersham™ Protran® 0.45 μM ...
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bioRxiv - Biophysics 2022Quote: ... were applied to the PAGE lane (10 wells, Any kD Mini-PROTEAN TGX Protein Gels, BioRad). The cathodic and anodic buffers were 25 mM Tris ...
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bioRxiv - Cancer Biology 2022Quote: ... Proteins were then separated using SDS-PAGE under denaturing conditions (4–20% Mini-PROTEAN TGX Gels) and transferred onto nitrocellulose membranes by semi-dry turbo blot (BioRad Laboratories) ...
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bioRxiv - Biophysics 2022Quote: ... The reactions were analyzed on denaturing polyacrylamide gel electrophoresis (Mini-PROTEAN TBA-Urea Precast Gels, BioRad), run for 30 minutes at 250 V ...
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bioRxiv - Bioengineering 2022Quote: ... and the equivalent of 10 μg of chlorophyll was loaded per lane on a Tris-Glycine based SDS-gel (Mini-Protean®TGX ...
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bioRxiv - Biochemistry 2022Quote: ... The protein concentration was measured on Tecan (Schoeller) and approximately 20 μg of protein lysate was loaded on the 10-well protein gels (Mini-PROTEAN TGX, Bio-Rad) and run at 120 V ...
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Dynamic states of eIF6 and SDS variants modulate interactions with uL14 of the 60S ribosomal subunitbioRxiv - Biochemistry 2022Quote: ... Quenched crosslinking reactions were then run on a Bio-Rad precast 4-20% gel (Mini-Protean TGX) at 150 V for 45 min ...
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bioRxiv - Cell Biology 2022Quote: ... 10 ug of protein was loaded onto precast gel (4561095, 4–20% Mini-PROTEAN® TGX™ Precast Protein Gels ...
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bioRxiv - Developmental Biology 2022Quote: ... 20 μL sample was run on an SDS-PAGE gel (Mini-PROTEAN TGX ...
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bioRxiv - Cell Biology 2022Quote: ... Whole-cell lysates were subjected to SDS–PAGE on 4–20% gradient gels (Mini-PROTEAN TGX; Bio-Rad). Proteins were transferred to polyvinylidene difluoride (PVDF ...
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bioRxiv - Cell Biology 2022Quote: ... Twenty to 40 µl of the lysate were loaded into a 12 % polyacrylamide gel for SDS-PAGE electrophoretic (BioRad. Mini-PROTEAN) separation of proteins ...
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bioRxiv - Cell Biology 2022Quote: Equal concentration of proteins from EV samples (10μg) were loaded onto precast SDS-polyacrylamide gels (BioRad, Mini Protean TGX Cat# 456-1086) and transferred to Immobilon-E PVDF Transfer membrane (Sigma ...
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bioRxiv - Plant Biology 2022Quote: ... Samples were resolved by SDS-PADE (precast 4-20% Mini-PROTEAN® TGX™: 4561095) assembled in gel tank apparatus (Mini-PROTEAN® system ...
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bioRxiv - Physiology 2022Quote: For the separation of myosin heavy chain isoforms a large format SDS-PAGE setup with integrated cooling core was used (Protean II xi, Bio-Rad Laboratories, Inc.). A 5% stacking gel ...
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bioRxiv - Microbiology 2022Quote: ... Equal amounts of total protein were loaded on a 4-20% gradient SDS-PAGE gel (Mini Protean TGX, Bio-Rad), then blotted onto nitrocellulose membranes and probed with anti-villin mouse monoclonal antibody (1:1000 ...
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bioRxiv - Microbiology 2022Quote: ... the denaturalized proteins were separated in a 12% SDS-PAGE precast gel (Mini-PROTEAN TGX Precast Gels, Bio-Rad). Electrophoresis was run at 250 V (PowerPac Basic power supply ...
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bioRxiv - Microbiology 2022Quote: ... The pellet obtained was dissolved in 10 μL of 20 mM Tris-HCl (pH 7.2) and 10 μL of Laemmli sample buffer (Mini-PROTEAN® Tetra Cell-Bio-Rad). The solubilized proteins were heated (95 °C ...
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bioRxiv - Microbiology 2022Quote: ... The proteins in the lysates were separated by SDS-polyacrylamide gel electrophoresis (SDS-PAGE) electrophoresis using gradient (4–20 %) PAGE gels (Bio-Rad, Mini-PROTEAN TGX), with a molecular mass marker (Bio-Rad ...
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bioRxiv - Microbiology 2022Quote: ... Proteins were run on a 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis and the transfer to a nitrocellulose membrane (Amersham™ Protran®) was performed in a wet system (Transfer chamber mini-Protean, BioRad). Membranes were blocked with 5% low fat powdered milk (Roth ...
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bioRxiv - Microbiology 2022Quote: ... loaded onto SDS-PAGE gels (Mini-PROTEAN Precast Protein Gels ...
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bioRxiv - Plant Biology 2022Quote: ... 20 μg of protein (15-well gels; Bio-Rad Mini-PROTEAN tetra system) was analyzed for MAPK phosphorylation by immunoblotting using the primary antibody anti-pERK MAPK (Phospho-p44/p42 MAPK ...
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bioRxiv - Microbiology 2022Quote: ... 20 μg of proteins of each sample or both input and eluates from the IPs were heated in Laemmli loading buffer at 95°C for 5 min and loaded on a SDS-polyacrylamide electrophoresis gel (Bio-Rad 4–20% Mini-PROTEAN® TGX Stain-Free™ Protein Gels ...
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bioRxiv - Cancer Biology 2021Quote: ... 30 μg of denatured proteins were separated by SDS-PAGE (Mini-PROTEAN TGX Gels 10%, Bio-Rad Laboratories) and transferred to 0.45 μm nitrocellulose membranes ...
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bioRxiv - Bioengineering 2021Quote: ... from Bio-Rad (4–20% Mini-PROTEAN® TGX™ Precast Protein Gels ...
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bioRxiv - Cancer Biology 2021Quote: ... Samples were cooled briefly and loaded onto 4-20% pre-cast gels (Bio-Rad Mini-PROTEAN TGX Gels Cat# 4561095) and run at 100-120V using the Bio-Rad Mini-PROTEAN Tetra System ...
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bioRxiv - Molecular Biology 2021Quote: ... for 5 min at 120 V (Bio-Rad Mini-PROTEAN®). The gel was stained following the protocol of Bio-Safe Coomassie Brilliant Blue G-250 and was destained overnight (details in Supplementary file 2) ...
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bioRxiv - Molecular Biology 2021Quote: ... 25 μg of total protein extracts were run on a gradient (15-4%) Polyacrylamide gel (MINIi-PROTEAN TGX Precast Gels, Biorad). Proteins were transferred on nitro-cellulose membrane and checked with Red Ponceau staining for efficient transfer ...
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bioRxiv - Neuroscience 2021Quote: ... 10 μg of protein per sample were loaded and separated by SDS-PAGE on 4-15 % gradient stain-free tris-glycine gels (Mini Protean TGX, BioRad, Hercules, CA), transferred to low fluorescence PVDF membranes (45 μm ...
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bioRxiv - Neuroscience 2021Quote: ... Proteins were separated in 10% polyacrylamide gels (Mini-PROTEAN TGX Stain-Free Gels, Bio-Rad) and transferred to polyvinyl difluoride membranes (Bio-Rad ...
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bioRxiv - Molecular Biology 2021Quote: ... or 4-15% gel (Mini-PROTEAN® TGX Stain-Free™; BIORAD). Proteins were transferred to nitrocellulose membranes (Amersham Protran 0.2um nc ...
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bioRxiv - Molecular Biology 2021Quote: ... Equal volumes of leaf protein were separated by SDS-PAGE (Mini-PROTEAN® TGX stain-free™ precast gels ...
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bioRxiv - Microbiology 2021Quote: ... run on a native gel (12% Mini-PROTEAN® TGX™ Precast Protein Gels, BioRad), and transferred to a membrane using BioRad’s Trans-Blot Turbo Transfer System (high molecular weight settings) ...
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bioRxiv - Molecular Biology 2021Quote: ... Proteins were separated on 4– 20% gradient gels (Mini-PROTEAN TGX; Bio-Rad, Hercules, CA) and transferred to nitrocellulose membrane (Bio-Rad) ...
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bioRxiv - Microbiology 2021Quote: ... Samples were boiled at 65 °C for 10 minutes and separated by SDS-Page (Bio-Rad 4–20 % Mini-PROTEAN® TGX Stain-Free™ protein gel), visualized by InstantBlue protein stain (Expedeon ...
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bioRxiv - Biochemistry 2021Quote: ... 10 μg of total protein was run on a 4–15% SDS-PAGE gel (Mini-PROTEAN TGX Precast Protein Gel; Bio-Rad). Proteins were then transferred onto a PVDF membrane using a Trans-Blot® SD Semi-Dry Transfer Cell (Bio-Rad ...
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bioRxiv - Bioengineering 2021Quote: ... Fractions were evaluated by SDS-PAGE (4-20% Mini-PROTEAN TGX, ThermoFisher), pooled ...
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bioRxiv - Biochemistry 2021Quote: ... 10 µl samples were first run in SDS-PAGE (Mini-Protean TGX 4-20% gradient gel, BioRad). The proteins were then transferred to a nitrocellulose membrane (Trans-Blot Turbo ...
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bioRxiv - Developmental Biology 2021Quote: ... Around 40 µg of proteins were loaded per lane of a 4-12% precast gel (Bio-Rad Mini-PROTEAN®). Proteins were transferred on PVDF-membrane and blocked for 1h with 5% skimmed milk ...
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bioRxiv - Physiology 2021Quote: ... equal amounts of protein were separated by SDS-PAGE (Mini-PROTEAN® TGX; Bio-Rad, USA), transferred onto PVDF membranes (GE Healthcare ...
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bioRxiv - Neuroscience 2021Quote: ... Thirty or 20 microgramms were respectively loaded on 4-20% precast gel (Mini-Protean TGX precast gels, Bio-Rad) for APP and tau proteins ...
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bioRxiv - Neuroscience 2021Quote: A 30 μL aliquot of either medium or lysate for each sample was heated at 95 °C for 10 min and loaded on two separate SDS-PAGE gels (Any kD™ Mini-PROTEAN® TGX Stain-Free Protein Gels; BioRad). Proteins were electrophoretically transferred to polyvinylidene fluoride (PVDF ...
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bioRxiv - Microbiology 2021Quote: ... Protein concentration was then checked via SDS-PAGE (Bio-Rad 4–20 % Mini-PROTEAN® TGX Stain-Free™ protein gel) and InstantBlue protein stain (Expedeon ...
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bioRxiv - Microbiology 2021Quote: ... (Protean 3D® ...
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bioRxiv - Microbiology 2021Quote: The eluted IP samples were separated by molecular weight using SDS-PAGE (precast 4-20% Mini-PROTEAN TGX Stain-Free Protein Gels ...
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bioRxiv - Physiology 2021Quote: ... Samples (10 µg) were resolved on 4-20% polyacrylamide gels (Mini Protean TGX Stain-Free gels, BioRad) and transferred to nitrocellulose membrane with Trans-Blot® Turbo™ Transfer system (Bio-Rad) ...
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bioRxiv - Immunology 2021Quote: ... with 0.5 μl of b-mercaptoethanol per well and heated at 70°C for 10 minutes before separation on a polyacrylamide gel (Bio-Rad Mini-PROTEAN TGX Gel 4-15%) and transferred to a PVDF membrane ...
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bioRxiv - Cell Biology 2021Quote: ... Proteins were resolved in SDS-PAGE gel (4-20% gradient polyacrylamide gel electrophoresis, Mini-PROTEAN® Precast Gels, Biorad). Blots were blocked for 1 hour with 5% non-fat dry milk in PBS/0.1% tween-20 (Sigma Aldrich) ...
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bioRxiv - Cell Biology 2021Quote: ... Purified septin complexes were analyzed by SDS-PAGE using 4-20% precast polyacrylamide gels (Mini-PROTEAN TGX Gels from Bio-Rad, 4561095), or hand-casted 12% or 15% Tris-glycine polyacrylamide gels ...
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bioRxiv - Cell Biology 2021Quote: ... ran on a 4-20 % protein gel (Bio-Rad Mini-PROTEAN TGX, #4561093), stained with InstantBlue® (Expedeon ...
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bioRxiv - Cell Biology 2021Quote: ... 10 µg of mESC lysate were separated on 4–12% or 10% SDS-PAGE gels (pre-cast NuPage® GE healthcare or Mini-Protean TGX Stain free precast gels ...