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Citations for Protean :
101 - 150 of 168 citations for Recombinant Human IL21 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2019Quote: ... and analysed for protein content using 12% SDS-PAGE gels (Mini-PROTEAN TGX Stain-Free Precast Gels, Bio-Rad). Protein gels were imaged using a BioRad Gel Doc EZ Imager and relative protein band intensity was determined using the BioRad ImageLab software.
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bioRxiv - Cell Biology 2021Quote: ... Proteins were resolved in SDS-PAGE gel (4-20% gradient polyacrylamide gel electrophoresis, Mini-PROTEAN® Precast Gels, Biorad). Blots were blocked for 1 hour with 5% non-fat dry milk in PBS/0.1% tween-20 (Sigma Aldrich) ...
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bioRxiv - Bioengineering 2021Quote: ... A sample containing 1-20µg of protein was loaded into a 4-15% precast gel (Bio-Rad Mini-ProTEAN) and run at 60V for 20min followed by 160V for 1 hour ...
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bioRxiv - Molecular Biology 2020Quote: ... equal amounts of total protein were loaded onto 4–20% SDS-PAGE gels (Mini-PROTEAN TGX Gel, Bio-Rad). After SDS-PAGE ...
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bioRxiv - Biochemistry 2020Quote: ... EV proteins were electrophoresed on a 12.5 % polyacrylamide gel (50V, 30 min) to obtain a single band (Mini-Protean II electrophoresis cell ...
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bioRxiv - Neuroscience 2020Quote: ... Ten micrograms of proteins was loaded on a 4-20% precast polyacrylamide gels (Mini Protean gels, Bio Rad, France). Then ...
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bioRxiv - Microbiology 2022Quote: ... the denaturalized proteins were separated in a 12% SDS-PAGE precast gel (Mini-PROTEAN TGX Precast Gels, Bio-Rad). Electrophoresis was run at 250 V (PowerPac Basic power supply ...
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bioRxiv - Microbiology 2022Quote: ... The samples were then separated on a gradient gel (4-20% Mini-PROTEAN® TGX™ Precast Protein Gels) with standard running buffer (0.4% (w/v ...
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bioRxiv - Microbiology 2023Quote: ... Samples were then analyzed by 4-20% SDS-PAGE gels (Mini-PROTEAN TGX Precast Protein Gels, Bio-Rad™) alongside size standards (Precision Protein Plus ...
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bioRxiv - Plant Biology 2023Quote: ... 20 µL samples were separated by 4-15% SDS-PAGE (Mini-PROTEAN® TGX™ Precast Protein Gels, BioRad). A mixture of recombinant proteins was used as a standard ...
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bioRxiv - Cell Biology 2024Quote: Protein extracts dissolved in Lämmli sample buffer or SDS sample buffer were separated by SDS-PAGE (Biorad Mini Protean) and transferred to ethanol activated Amersham Hybond-P 0.45 µm PVDF membranes (Cytiva ...
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bioRxiv - Cell Biology 2024Quote: Equal protein amounts of cell lysates and mitochondria-enriched fractions were separated using SDS-PAGE gels (PreCast Mini-PROTEAN Tris Glycine TGX ...
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bioRxiv - Physiology 2020Quote: ... Equal amounts of protein were resolved on SDS-PAGE (10% Mini-PROTEAN® TGX Stain-Free™ Gels, Bio-Rad) and transferred to polyvinyl difluoride membranes (Bio-Rad) ...
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bioRxiv - Systems Biology 2019Quote: ... Equal amounts of protein were loaded onto the acrylamide gel (Bio-Rad Mini or Midi PROTEAN® TGX precast gels). For the transfer of proteins to the PVDF membrane ...
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bioRxiv - Developmental Biology 2021Quote: ... Around 40 µg of proteins were loaded per lane of a 4-12% precast gel (Bio-Rad Mini-PROTEAN®). Proteins were transferred on PVDF-membrane and blocked for 1h with 5% skimmed milk ...
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bioRxiv - Genetics 2022Quote: ... 60 μg of protein was separated on 10% SDS/PAGE (Mini-PROTEAN TGX Gels,12%,10-well, 4561043, BIO-RAD) and transferred onto nitrocellulose membrane (#1620115 ...
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bioRxiv - Pathology 2019Quote: ... and lysates were prepared as previously described. (Lennikov, Saddala et al. 2019) Thirty micrograms of total proteins were separated by SDS-PAGE gel (Mini-Protean Precast Acrylamide Gels ...
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bioRxiv - Cancer Biology 2022Quote: ... 40 μg of total protein was separated on a 4–20% SDS-PAGE gel (mini-PROTEAN TGX gels, Bio-Rad) along with PageRuler Prestained protein ladder (Cat ...
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bioRxiv - Microbiology 2022Quote: ... Equal amounts of total protein were loaded on a 4-20% gradient SDS-PAGE gel (Mini Protean TGX, Bio-Rad), then blotted onto nitrocellulose membranes and probed with anti-villin mouse monoclonal antibody (1:1000 ...
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bioRxiv - Microbiology 2024Quote: ... 10 μg of total protein for both NT and Rab siRNA-transfected samples were resolved in 4-20% gradient SDS-PAGE (Mini-PROTEAN Gel ...
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bioRxiv - Molecular Biology 2023Quote: 20-25μg of protein lysate with 4x Laemmli buffer (cat# 1610747; Bio-Rad Laboratories was separated on 4-15% Mini-PROTEAN TGX Precast Protein Gels (cat# 4561086 ...
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bioRxiv - Molecular Biology 2023Quote: ... 20 µg of extracted proteins mixed with 2× SDS sample buffer were separated on 10% SDS PAGE gel (Mini PROTEAN TGX Precast Gel ...
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bioRxiv - Cancer Biology 2023Quote: ... Ten micrograms of total protein extract underwent electrophoresis in stain-free 4–15% gradient Tris-glycine SDS-polyacrylamide gels (Mini PROTEAN® TGX Stain-Free™ Gels ...
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bioRxiv - Physiology 2023Quote: ... Total protein (5∼15 µg) was loaded and separated by 4–20% pre-made SDS-PAGE gels (#4568095, BioRad, Mini-PROTEAN TGX Stain-Free Precast Gels ...
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bioRxiv - Cancer Biology 2023Quote: ... 15 micrograms of total protein were separated using stain-free 4–15% gradient Tris-glycine SDS-polyacrylamide gels (Mini PROTEAN® TGX Stain-Free™ Gels ...
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bioRxiv - Synthetic Biology 2024Quote: ... Protein purity and size were validated on an SDS-PAGE gel (Bio-Rad Mini-PROTEAN TGX and Mini-TETRA cell). Purified proteins were stored at –80°C.
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bioRxiv - Molecular Biology 2021Quote: ... 25 μg of total protein extracts were run on a gradient (15-4%) Polyacrylamide gel (MINIi-PROTEAN TGX Precast Gels, Biorad). Proteins were transferred on nitro-cellulose membrane and checked with Red Ponceau staining for efficient transfer ...
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bioRxiv - Cell Biology 2022Quote: ... 25µg of protein was loaded per lane into an SDS-PAGE gel (Bio-Rad Mini-Protean pre-cast gels 4-20%) and gels were run at 160V for approximately 90 minutes ...
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bioRxiv - Immunology 2019Quote: ... and equal amounts of proteins were resolved by electrophoresis on 12% Tris-HCl gels (Mini-PROTEAN TGX gels; Bio-Rad, #4561043) and transferred onto polyvinylidene difluoride membranes (Trans-Blot Turbo Transfer Pack ...
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bioRxiv - Developmental Biology 2021Quote: Proteins extracted from Padi4CTL and Padi4CKO were subjected to SDS–PAGE (4–20% Mini-PROTEAN® TGX™ Precast gel, Biorad) and then transferred onto a nitrocellulose membrane ...
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bioRxiv - Cell Biology 2020Quote: Proteins were separated on a polyacrylamide gel with 10% of sodium dodecyl sulfate(SDS) (Mini Protean III; Bio-Rad, Richmond, CA), and they were transferred for 2 hours at 48°C to nitrocellulose membranes of 0.45 μm ...
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bioRxiv - Genomics 2021Quote: ... Proteins were subjected to electrophoresis through a 12% Glycine SDS-PAGE gel (Mini-PROTEAN TGX Stain-Free Precast gel, Bio-Rad) and transferred onto a polyvinylidene difluoride (PVDF ...
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bioRxiv - Plant Biology 2023Quote: ... Ten micrograms of denatured microsomal proteins were separated by SDS-PAGE using 10% polyacrylamide gel (Mini-PROTEAN TGX precast gel, Biorad) and tris/glycine buffer ...
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bioRxiv - Physiology 2024Quote: ... 5 ug (BAT) or 10 ug (ingWAT) of protein was separated on a 12% polyacrylamide gel (Mini-PROTEAN® TGXTM, BioRad). Proteins were then transferred to a PVDF membrane (BioRad ...
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bioRxiv - Cancer Biology 2020Quote: ... Equal amounts of proteins (30-60µg) were separated via SDS polyacrylamide gel electrophoresis (SDS-PAGE) using precast 10% polyacrylamide gels (Mini-Protean TGX, Bio-Rad). Proteins were transferred onto polyvinylidene fluoride (PVDF ...
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bioRxiv - Cell Biology 2022Quote: ... Appropriate volumes of samples were diluted to provide equal protein amounts and used for SDS-PAGE (BIO-RAD, Mini-PROTEAN TGX Gels). Western blot analysis was performed on 3 samples for wild-type and banprw337 mutants ...
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bioRxiv - Synthetic Biology 2019Quote: ... The purity and size of the proteins were validated on SDS-PAGE gel (Mini-PROTEAN TGX and Mini-TETRA cell, Bio-Rad). Purified proteins were stored at –20° C ...
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bioRxiv - Microbiology 2020Quote: ... Samples were checked for purity and presence of protein using 15% SDS-PAGE (Polyacrylamide gel electrophoresis, Mini Protean 3 System, Bio-Rad) and Coomassie Brilliant Blue (Merck ...
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bioRxiv - Cell Biology 2022Quote: Equal concentration of proteins from EV samples (10μg) were loaded onto precast SDS-polyacrylamide gels (BioRad, Mini Protean TGX Cat# 456-1086) and transferred to Immobilon-E PVDF Transfer membrane (Sigma ...
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bioRxiv - Cancer Biology 2023Quote: ... Equal amounts of proteins (30-60µg) were resolved via SDS polyacrylamide gel electrophoresis (SDS-PAGE) using precast polyacrylamide gradient gels (Mini-Protean TGX, Bio-Rad) and transferred onto polyvinylidene fluoride (PVDF ...
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bioRxiv - Cancer Biology 2019Quote: ... Nuclear extracts containing the total of 40 µg protein were separated on a 10% Tris-SDS PAGE gel (Bio-Rad Mini-PROTEAN® TGX). Gels were transferred on nitrocellulose membranes using Bio-Rad TransBlot system ...
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bioRxiv - Plant Biology 2019Quote: Nectars and the protein extracts were separated by stain-free 1D SDS-PAGE (Bio-Rad’s Mini PROTEAN® TGX Stain-Free™ Gels). For western blot assays ...
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bioRxiv - Microbiology 2020Quote: ... Cellular lysates were separated by SDS-PAGE using 15-20 µg total cellular protein per lane (4-20% Mini-PROTEAN TGX gels (BioRad), 120 V for 1 hour) ...
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bioRxiv - Synthetic Biology 2021Quote: ... The purity and size of the proteins were validated on a SDS-PAGE gel (Mini-PROTEAN TGX and Mini-TETRA cell, Bio-Rad). Purified proteins were stored at –20° C.
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bioRxiv - Synthetic Biology 2023Quote: ... The purity and size of the proteins were validated on an SDS-PAGE gel (Mini-PROTEAN TGX and Mini-TETRA cell, Bio-Rad). Purified proteins were stored at 4° C.
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bioRxiv - Biochemistry 2023Quote: Protein purity and homogeneity was assessed using both denaturing and native polyacrylamide gel electrophoresis (Mini-PROTEAN TGX Stain-Free Precast Gels Bio-Rad). FabWT F1 and SER variants (S1-S7 ...
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bioRxiv - Genetics 2024Quote: ... Protein samples were prepared and denatured in Laemmli Buffer and SDS-PAGE was performed under reducing conditions (Mini-PROTEAN® Tetra, Bio-Rad) before transfer onto nitrocellulose membranes ...
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bioRxiv - Synthetic Biology 2024Quote: ... The purity and size of the proteins were validated on an SDS–PAGE gel (Mini-PROTEAN TGX and Mini-TETRA cell, Bio-Rad). Purified proteins were stored at –20 °C.
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bioRxiv - Bioengineering 2020Quote: ... 1 µg of each protein sample was separated in one dimension using 4-20% tris-glycine gels (Mini-PROTEAN TGX, Bio-Rad Laboratories, Inc.). A 1:6 dilution of the PageRuler Plus pre-stained protein ladder (10 µl ...
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bioRxiv - Neuroscience 2022Quote: ... The proteins (20-30 μg) of the tissue protein samples were subjected to 12% sodium dodecyl sulfate polyacrylamide gel electrophoresis (Bio-Rad mini-PROTEAN TGX Gels, # 4561044) and transferred to polyvinylidene fluoride (PVDF ...