Labshake search
Citations for Froggabio :
1 - 50 of 74 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2024Quote: ... Regular PCR was performed using 2X Taq FroggaMix (Froggabio, Concord, ON, Canada) with gene-specific primers (Table 1).
-
bioRxiv - Developmental Biology 2024Quote: RNA was isolated from a pool of 10 embryos using The GENEzol™ TriRNA Pure Kit (FroggaBio). RNA was then directly used in the Luna® Universal One-Step RT-qPCR Kit (New England BioLabs) ...
-
bioRxiv - Developmental Biology 2024Quote: RNA was isolated from a pool of 10 embryos at 50% epiboly using the GENEzol™ TriRNA Pure Kit (FroggaBio). RNA samples were DNase treated using the Invitrogen™ DNA-free™ DNA Removal Kit (Thermo Fisher Scientific ...
-
bioRxiv - Physiology 2024Quote: ... using SensiFAST SYBR No-ROX Kit (Cat no. BIO-98050, Bioline, Froggabio, Canada). The amplification reaction contained 1 μL of diluted cDNA ...
-
bioRxiv - Evolutionary Biology 2024Quote: ... the plasmid DNA was extracted from bacteria using a plasmid extraction kit (FroggaBio) to validate the plasmid single mutant libraries ...
-
bioRxiv - Genetics 2024Quote: ... and ran samples on 2% agarose gels stained with RedSafe (FroggaBio) at 95V for 30 min and visualized under UV light (Bio-Rad ChemiDoc Imaging System ...
-
bioRxiv - Physiology 2024Quote: ... Total RNA was extracted from cultured cells using a commercial kit following the manufacturer’s instructions (Genaid, Froggabio or Nagel-Macherey). One μg of total RNA was reverse-transcribed as per the manufacturer’s instructions (VWR and BioRad) ...
-
bioRxiv - Biochemistry 2024Quote: ... Amplicons were visualized on a 1% agarose gel stained with RedSafe Nucleic Acid Staining Solution (FroggaBio). pET15b-AtGAD1 was transformed into E ...
-
bioRxiv - Microbiology 2024Quote: ... 5 µl of BLUelf prestained ladder with MW 5-245 kDa (FroggaBio) was loaded ...
-
bioRxiv - Microbiology 2024Quote: ... The above three PCR fragments were purified using the Gel/PCR purification kit (Froggabio, CA) and inserted into EcoRI/XbaI-digested pUC19 using the NEBuilder® HiFi DNA Assembly Cloning Kit ...
-
bioRxiv - Physiology 2024Quote: ... and separated via SDS-PAGE alongside the IRIS11 Prestained Protein Ladder (FroggaBio, Toronto, ON, Canada), in a mini-PROTEAN Tetra System (BioRad ...
-
bioRxiv - Synthetic Biology 2024Quote: DNA concentrations were measured by fluorometry using the DeNovix dsDNA Broad Range Assay (FroggaBio, catalog number: DSDNA-BROAD-EVAL) and purity ratios were assessed using a Nanodrop spectrophotometer ...
-
bioRxiv - Cancer Biology 2024Quote: ... or MicroChemi Chemiluminescent imager using the GelCapture Software (version 2.2.2.0) (FroggaBio Inc.). Digital images were quantified using Image J (version 1.52p ...
-
bioRxiv - Cancer Biology 2024Quote: ... FroggaBio BLUelf Prestained Protein ladder (FroggaBio, Concord, Om, Canada) was used to estimate protein molecular weight ...
-
bioRxiv - Cell Biology 2024Quote: ... stained with Red Safe dye (Froggabio: 21141) using a Sub-Cell GT Agarose Gel Electrophoresis System (BioRad ...
-
bioRxiv - Neuroscience 2023Quote: ... mRNA was reverse transcribed into single-stranded cDNA using the SensiFAST cDNA Synthesis Kit for RT-PCR (Cat. No BIO-65054, FroggaBio). After conversion to cDNA ...
-
bioRxiv - Neuroscience 2023Quote: ... real-time PCR was performed using the SensiFAST SYBR No-ROX Kit (Cat. No BIO-98005, FroggaBio) using a Rotor-Gene 6000 (Corbett Life Science) ...
-
bioRxiv - Cell Biology 2023Quote: ... The cells were counted using the CellDrop automated cell counter (Froggabio, ON). The lysis buffer used contained 100 mM HEPES (pH= 8-8.5 ...
-
bioRxiv - Plant Biology 2023Quote: ... was performed on an Agilent AriaMx RealTime PCR instrument using the SensiFAST SYBR No-ROX qPCR 2X master mix (FroggaBio, Canada) according to the manufacturer’s protocol ...
-
bioRxiv - Immunology 2023Quote: ... and DNA isolation kits were from FroggaBio (Concord, Canada), and all laboratory chemicals were from Bioshop Canada (Burlington ...
-
bioRxiv - Immunology 2023Quote: ... cDNA was synthesized from 1 µg RNA using SensiFAST cDNA Synthesis Kit (FroggaBio; Concord, Canada). qPCR was performed using a QuantStudio™ 7 Flex Real-Time PCR System in conjunction with a SybrGreen System (Thermo Scientific ...
-
bioRxiv - Molecular Biology 2023Quote: ... samples were visualized on a on a 1% agarose gel with a 100 bp ladder (Froggabio) for reference ...
-
bioRxiv - Molecular Biology 2023Quote: ... PCR amplification was verified on a 1% agarose gel with a 100 bp ladder (Froggabio) for reference.
-
bioRxiv - Bioengineering 2023Quote: ... Gel was casted by the addition of 2.4 g agarose (FroggaBio, Cat#A87) into 120 mL of 0.5x TBE ...
-
bioRxiv - Cancer Biology 2023Quote: ... Samples were denatured at 100°C and separated on 10% SDS-PAGE gels with BLUeye Prestained Protein Ladder (FroggaBio, Concord ON). Protein was then semi-dry transferred to a PVDF membrane and incubated in Li-Cor Intercept® Blocking Buffer (Cedarlane ...
-
bioRxiv - Cancer Biology 2023Quote: ... PCR amplification of the Jak3 pseudokinase domain was performed using MyTaq HS Red Master Mix (FroggaBio) and primers FWD ...
-
bioRxiv - Synthetic Biology 2023Quote: ... we amplified the cassette by PCR using Taq (Froggabio T-500) or Q5 (NEB M0491 ...
-
bioRxiv - Neuroscience 2023Quote: ... RT-qPCR analysis was carried out using the Lo-Rox SYBR 2x mix (FroggaBio Inc #CSA-01195) under the following conditions ...
-
bioRxiv - Cell Biology 2023Quote: ... Cells were routinely tested for mycoplasma (FroggaBio, 25235). For amino acid starvation experiments ...
-
bioRxiv - Biochemistry 2022Quote: ... except for an ubiquitin specific primary antibody (1:1000 dilution; UBCJ2, Mono- and polyubiquinated conjugates monoclonal antibody, FroggaBio).
-
bioRxiv - Cell Biology 2022Quote: ... according to LipoD293 reagent instructions (FroggaBio cat #SL100668.1): Lentiviral packaging plasmids pMD2.g (Addgene #12269) ...
-
bioRxiv - Evolutionary Biology 2022Quote: ... were transfected into CHO-K1 cells grown to 80-90% cell confluency using the transfection reagent PolyJetTM (FroggaBio, Concord Ontario), according to the manufacturer protocol ...
-
bioRxiv - Cell Biology 2022Quote: ... Detection was performed with Western Lightning® Plus-ECL substrate (NEL103001EA, FroggaBio) and exposed to x-ray film (sc-201697 ...
-
bioRxiv - Synthetic Biology 2022Quote: ... 2.4g of agarose (FroggaBio, Cat#A87) was added to 120 mL 0.5X TBE and microwaved until dissolved ...
-
bioRxiv - Molecular Biology 2022Quote: ... An aliquot of both cell lines was subjected to mycoplasma testing (e-Myco™ VALiD Mycoplasma PCR Detection Kit, Froggabio, 25239) and confirmed negative.
-
bioRxiv - Neuroscience 2022Quote: ... Cells were routinely tested for mycoplasma using the e-myco VALID mycoplasma testing kit (FroggaBio, 25239). Cells were maintained at 37°C temperature and 5% CO2 in a humidified atmosphere.
-
ZMYM2 is essential for methylation of germline genes and active transposons in embryonic developmentbioRxiv - Molecular Biology 2022Quote: ... and first-strand cDNA was generated using the SensiFast cDNA Synthesis kit (FroggaBio, DD-BIO-65053). Quantitative PCR was then performed using PowerUP SYBR™ Green PCR (Invitrogen ...
-
bioRxiv - Microbiology 2022Quote: ... The resulting plasmids were then purified using the Geneaid High-Speed Mini Kit (Froggabio, Concord, ON) and then sequenced at the Université Laval sequencing center ...
-
bioRxiv - Microbiology 2022Quote: ... RNA extractions were performed using either a Presto Mini RNA Yeast Kit from FroggaBio (cat. RBYD050) or an RNeasy Mini Kit from Qiagen (cat ...
-
bioRxiv - Developmental Biology 2022Quote: ... Proteins were separated on 10% SDS-PAGE gels and transferred to 0.22 μm PVDF membrane (FroggaBio, USA) by an electric current at 300 mA for 70 min ...
-
bioRxiv - Molecular Biology 2022Quote: ... and imaged on a MicroChemi 2.0 Imager (FroggaBio). Antibodies and concentrations used for western blots can be seen in Appendix 3.
-
bioRxiv - Developmental Biology 2022Quote: ... 10 μL of SensiFAST SYBR No-ROX Mix (FroggaBio, BIO-98050) and 1 μL of cDNA ...
-
bioRxiv - Molecular Biology 2022Quote: ... and detected with MicroChemi4.2 (FroggaBio).
-
bioRxiv - Immunology 2022Quote: ... Reverse transcriptase quantitative polymerase chain reaction (RT-qPCR) was performed using SensiMix SYBR No-Rox master mix (Froggabio, Toronto, Canada) on a QuantStudio5 instrument (Applied biosystems ...
-
bioRxiv - Molecular Biology 2021Quote: ... DNA was amplified using primers specific to different genetically engineered alleles (Supplementary Table 1) using FroggaMix (FroggaBio FBTAQM). PCR conditions started with 95°C for 3 min ...
-
bioRxiv - Molecular Biology 2021Quote: ... RNA was extracted from a pool of five heads for each treatment using the GENEzol™ TriRNA Pure Kit (Froggabio). RNA samples were DNase treated using the Invitrogen™ DNA-free™ DNA Removal Kit (Thermo Fisher Scientific) ...
-
bioRxiv - Molecular Biology 2021Quote: ... a 10 μl qPCR reaction is set up using SensiFAST SYBR No-ROX kit (FroggaBio/Bioline, catalogue number BIO-98050), forward and reverse primers (Table 2) ...
-
bioRxiv - Immunology 2021Quote: ... and filtered through a 0.22 μm PES filter (FroggaBio, Concord, Canada) prior to storage at −80 °C until use.
-
bioRxiv - Immunology 2021Quote: ... filtered through a 0.22 μm PES filter (FroggaBio), and aliquoted into sterile 1.5 mL microfuge tubes for storage at −80 °C.
-
bioRxiv - Immunology 2021Quote: ... and RNA quality was examined by electrophoresing 1 μg of RNA on a 1% agarose gel containing 1% bleach (Aranda et al., 2012) and 1 × RedSafe nucleic acid staining solution (FroggaBio) in 1 × TAE buffer at 100 V for 35 min ...