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Citations for Bioline :
51 - 100 of 480 citations for hsa mir 635 RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... 4 were generated from 200 ng genomic DNA (gDNA) using junction-specific primers (Table S4) by a two-step nested PCR with Velocity polymerase (Bioline). The first PCR reaction was performed for 15 cycles with 60 °C annealing and 30 s elongation and then purified with AMPure XP PCR beads (Beckman Coulter) ...
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bioRxiv - Plant Biology 2021Quote: ... Amplicons for sequencing were generated by PCR using degenerate primers (see Supplemental Table 1) for each locus using My Taq HS mix (Bioline) and gel purification ...
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bioRxiv - Neuroscience 2019Quote: ... PCR reactions were performed using 5μl cDNA mix and 200nM custom-designed primers (Table 1) and SensiMix (Bioline, London, UK). qPCR data were analysed using ΔCt methods as described previously [Isles et al. ...
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bioRxiv - Molecular Biology 2020Quote: ... and 1 μL of DNA was used in a PCR reaction using 0.3 μΜ primers and 5U of MyTaq DNA polymerase (Bioline).
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bioRxiv - Microbiology 2021Quote: ... Viral vRNA and mRNA were detected by polarity-specific quantitative RT-PCR using the SensiFast Probe No-ROX one-step kit (Bioline). Each 20 µL reaction contained 5 μl of RNA ...
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bioRxiv - Immunology 2021Quote: ... The real-time quantitative PCR (RT-qPCR) analysis was carried out by using the 2x SensiMix PLUS SYBR No-ROX Kit (Bioline) in a Rotor-Gene 6000 (Corbett ...
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bioRxiv - Microbiology 2021Quote: ... the K13 locus was amplified directly from whole blood using the primer pair p26+p27 (Table S9) and the MyTaq™ Blood-PCR Kit (Bioline). Primer pairs p39+p40 and p50+p51 were used to amplify fd and mdr2 ...
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bioRxiv - Synthetic Biology 2021Quote: ... The DNA was used as template for PCR amplification of the targeted sites with primers listed on Supplementary Table 3 and using MyTaq™ DNA Polymerase (Bioline). Subsequently ...
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bioRxiv - Cancer Biology 2020Quote: ... Primers were designed using NCBI primer blast (Table 3) and qRT-PCR was performed using SensiFAST™ SYBR Lo-ROX Kit (#BIO-94020, Bioline) on the BIO-RAD CFX38 Real time PCR machine ...
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bioRxiv - Physiology 2021Quote: ... the K13 locus was amplified directly from whole blood using the primer pair p26+p27 (Supplementary file 5) and the MyTaq™ Blood-PCR Kit (Bioline). Primer pairs p39+p40 and p50+p51 were used to amplify fd and mdr2 ...
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bioRxiv - Molecular Biology 2020Quote: ... using Random Primer (Bioline Inc.) by incubating the reaction mixture at 50°C for 30 minutes ...
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bioRxiv - Cell Biology 2021Quote: ... using random primers (Bioline, UK). One µl of cDNA was specifically and quantitatively amplified using Biotool 2x SybrGreen qPCR master mix (Stratech ...
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bioRxiv - Molecular Biology 2022Quote: ... using Random Primer (Bioline Inc.) by incubating the reaction mixture at 50°C for 30 minutes ...
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bioRxiv - Immunology 2021Quote: ... The master mix was prepared with 2.5 mL of 2X buffer containing Taq-polymerase was prepared from the TaqMan RT-PCR kit (Bioline #BIO-78005). From the kit ...
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bioRxiv - Microbiology 2020Quote: ... The final PCR primer concentration was 500 nM in a volume of 10 μl with the SensiFast SYBR No-ROX Kit (Bioline, London, UK). The following amplification program was used ...
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bioRxiv - Microbiology 2023Quote: ... SdhC and SdhD was performed with primers listed in Table 2 and the following reaction conditions: PCR amplification used MyTaq DNA Polymerase (Bioline, London, UK), with 0.25 μM each forward and reverse primer ...
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bioRxiv - Genomics 2020Quote: ... One-step reverse-transcription quantitative PCR (RT-qPCR) was prepared with 50 ng of RNA using the SensiFAST™ SYBR® No-ROX One-Step kit (Bioline) on an Eco™ Real-Time PCR System (Ilumina) ...
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bioRxiv - Cancer Biology 2020Quote: Real-time RT-PCR reactions were performed in 20ml volumes with 10 ml of SensiFAST SYBR Lo-ROX Kit (Bioline, Taunton, MA) 2ml of cDNA template and 0.5ml each of the forward and reverse primers of the gene of interest (GOI) ...
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bioRxiv - Plant Biology 2021Quote: ... Real-time quantitative reverse transcriptase PCR (RT-qPCR) was performed using a Biorad CFX384 Optics Module with SensiFAST SYBER No-ROX (Bioline Meridian Biosystems) using gene-specific primers listed in Supplementary Table 1 ...
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bioRxiv - Genomics 2021Quote: ... One-step reverse-transcription quantitative PCR (RT-qPCR) was performed with the SensiFAST™ SYBR® No-ROX One-Step kit (Bioline®) on an Eco™ Real-Time PCR System (Ilumina® ...
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bioRxiv - Microbiology 2021Quote: The origin of viral genes present in samples were determined by gene-specific reverse transcription polymerase chain reaction (RT-PCR) using a SensiFast Probe No-ROX one-step reverse transcription kit (Bioline, Meridian Biosciences, Ohio, USA). Each 20 µL reaction contained 5 μl of virus sample in 0.05% Triton-X 100 ...
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bioRxiv - Molecular Biology 2023Quote: ... This cDNA library was then PCR barcoded with PCR Primer 1.0 (5’-AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACG-3’) and a sample-specific barcode primer (5’-CAAGCAGAAGACGGCATACGAGATNNNNNNGTGACTGGAGTTCAGACGTG-3’) using MyTaq Red Mix (Bioline) for 15 cycles ...
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bioRxiv - Plant Biology 2019Quote: ... 0.4 μL 10 μM specific primer pairs (mixture of forward and reverse primers) was mixed with 10 μL SensiFAST SYBR (Bioline) mastermix and 9.6 μL of cDNA ...
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bioRxiv - Genetics 2023Quote: ... An 800–base pair region surrounding the target site of the Cypor sgRNA was amplified (forward primer 5′-GTTTGCGGGTGTTAGCTCTTC-3′; reverse primer 5′-TTGGTGGGTAAATCACACCGT-3′) using MyTaq Red Mix (Bioline). The amplicon was purified using the PCR clean-up and gel extraction kit (Macherey-Nagel ...
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bioRxiv - Cancer Biology 2021Quote: ... and oligo (dT)18 primers (Bioline #BIO-38029). The RT-qPCR reaction was performed using SensiFAST SYBR Hi-ROX Kit (Bioline #BIO-92020 ...
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bioRxiv - Molecular Biology 2021Quote: ... qPCR reactions were set up using sensiFAST SYBR Lo-ROX kit (Bioline). The qPCR was performed as described above for RIP-qPCR.
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bioRxiv - Cell Biology 2019Quote: ... qPCR reactions were set up using SensiMix 2x Mastermix (Bioline Cat# QT615-20) and oligonucleotides targeting genes of interest (IDT ...
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bioRxiv - Cell Biology 2021Quote: ... qPCR reactions were set up using SensiFast 2× Mastermix (Bioline Cat# BIO-94020) and oligonucleotides targeting genes of interest (IDT ...
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bioRxiv - Microbiology 2020Quote: ... Primers used in this analysis were obtained from Bioline Inc (Taunton ...
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bioRxiv - Molecular Biology 2020Quote: ... random hexamers primers and Tetro cDNA synthesis kit (Bioline) were used for cDNA synthesis and GAPDH was used as internal control ...
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bioRxiv - Molecular Biology 2023Quote: ... The final product was diluted to 1:20 and 2 µL of the dilution were used in each 10-µl RT-qPCR reaction along with 400 nM of primers gene- or isoform-specific primers (Table S4B) and 1X SensiFAST SYBR No-ROX Mix (Bioline, cat#BIO-98005). Quantitative PCR was performed in CFX384 Touch Real-Time PCR Detection System (Bio-Rad) ...
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bioRxiv - Plant Biology 2021Quote: ... RT-qPCR reactions using SYBR green chemistry (SensiFast; Bioline) were set up with a CAS-1200N robotic liquid handling system (Corbett Research ...
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bioRxiv - Molecular Biology 2020Quote: ... For RT-qPCR the SensiFAST SYBR Lo-ROX Kit (Bioline) was used ...
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bioRxiv - Microbiology 2019Quote: ... cDNA was generated by reverse-transcription using Sensifast RT (Bioline) at 25°C for 10 mins ...
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bioRxiv - Plant Biology 2021Quote: ... RT-qPCR reactions were made using SensiFAST master-mix (Bioline), in 20 μl volumes ...
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bioRxiv - Plant Biology 2019Quote: ... RT-qPCR reactions were made using SensiFAST master-mix (Bioline), each with 20 ng of cDNA ...
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bioRxiv - Genomics 2023Quote: ... Reverse transcription was carried out with Tetro RT enzyme (Bioline). The 20 µl RT reaction mix consisted of 2.5 µl of total RNA (at 300 ng/µl) ...
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bioRxiv - Synthetic Biology 2021Quote: ... We performed colony PCR using BioRed PCR mix (Bioline) to screen for genome modifications ...
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bioRxiv - Molecular Biology 2022Quote: ... RT-qPCR was performed using SensiFAST SYBR No-ROX Kit (Bioline) and 0.3 μM of forward and reverse primers ...
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bioRxiv - Molecular Biology 2022Quote: ... RT-qPCR was conducted with SensiFast SYBR lo- ROX Kit (Bioline) on a Quant Studio 6 Flex real-time PCR system (Thermo Fisher Scientific).
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bioRxiv - Microbiology 2022Quote: ... RT-qPCR was performed using the SensiFAST SYBR No-ROX kit (Bioline) according to the manufacturer’s instructions on a CFX96 Touch real-time PCR instrument (Bio-Rad) ...
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bioRxiv - Cancer Biology 2023Quote: ... RT–qPCR was performed using the SensiFAST Syber Low-ROX kit (Bioline) QuantStudio 3 RealTime qPCR (Applied Biosystems) ...
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bioRxiv - Genomics 2023Quote: ... PCR products purified with Isolate II PCR and Gel Kit (BIOLINE) and cloned into the entry vector pCR®8/GW/TOPO (#250020 Invitrogen ...
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bioRxiv - Genomics 2021Quote: ... 10 pmoles each primer and 0.25 μL (1.25 U) MyTaq DNA Polymerase (Bioline). Thermal cycling conditions were 95°C for 1 min followed by a 5-cycle touch-down (95°C for 30 s ...
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Increased ACTL6A Occupancy Within mSWI/SNF Chromatin Remodelers Drives Human Squamous Cell CarcinomabioRxiv - Molecular Biology 2021Quote: ... cDNAs were mixed with indicated primers and SensiFAST SYBR lo-ROX reagents (Bioline), and quantitative PCR (qPCR ...
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bioRxiv - Bioengineering 2021Quote: ... 400 nM of each primer and 1x SensiFast SYBR® No-ROX (Bioline) in the LightCycler 480 System (Roche ...
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bioRxiv - Molecular Biology 2022Quote: ... with primers for Rps26 and Klf2 genes using SensiFast No-Rox kit (Bioline). The expression of Klf2 was calculated using the 2-ΔΔCt ...
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bioRxiv - Physiology 2023Quote: ... 800 nM of forward and reverse primers and SensiFAST SYBR Lo-ROX (Bioline). We ran gene-specific qPCR plates and each sample was analysed in duplicate ...
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bioRxiv - Microbiology 2021Quote: ... After PCR verification in agarose gel and PCR clean-up using the ISOLATE II PCR and Gel Kit (Bioline), the remaining plasmid template was digested with DpnI at 37°C for 3h ...
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bioRxiv - Molecular Biology 2020Quote: ... and purified using the PCR Isolate II PCR and Gel Kit (Bioline) or by ExoSAP (for primers see Table S4) ...