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Citations for Bioline :
151 - 200 of 480 citations for hsa let 7f RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2020Quote: ... PCR reactions were conducted using MyTaq (Bioline) DNA polymerase ...
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bioRxiv - Genetics 2020Quote: ... or MyTaq™ Extract-PCR Kit (Bioline). gDNA was prepared from blastocysts in a 20 µL solution of 185.5 mM pH 8.3 Tris-HCl ...
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Psi promotes Drosophila wing growth through transcriptional repression of key developmental networksbioRxiv - Developmental Biology 2020Quote: ... PCR using MyTaq polymerase (Bioline BIO-21113) was performed with 3 long extension cycles followed by 17 short extension cycles as described (Vogel ...
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bioRxiv - Genetics 2020Quote: ... Genotyping was performed by PCR of genomic tail DNA using the Extract PCR kit (Bioline, cat. No. BIO-21127). All mice were kept on C57BL/6J (The Jackson Laboratory ...
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bioRxiv - Plant Biology 2022Quote: ... PCR products were purified from agarose gels using the ISOLATE II PCR and Gel Kit (Bioline, Memphis, TN, USA), and quantified in a NanoDrop One C Spectrophotometer (Thermo Scientific ...
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bioRxiv - Cancer Biology 2022Quote: ... cDNA was prepared for RT-qPCR using the SensiFAST cDNA preparation kit according to manufacturer instructions (Bioline #65054). 1μL of cDNA was used per RT-qPCR reaction prepared with SYBR Lo-ROX (Bioline #94020) ...
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bioRxiv - Molecular Biology 2023Quote: ... cDNA was prepared for RT-qPCR using the SensiFAST cDNA preparation kit according to manufacturer instructions (Bioline #65054). 1µL of cDNA was used per RT-qPCR reaction prepared with SYBR Lo-ROX (Bioline #94020) ...
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bioRxiv - Developmental Biology 2019Quote: ... 0.4 μM zebrafish genotyping primers (Extended Data Fig.5A, Table 2) and 1 x MyTaq Red DNA Polymerase (Bioline) according to the manufacturer’s protocol in a T100 thermal cycler ...
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bioRxiv - Genetics 2020Quote: ... 1 μl forward and reverse primers (10 mM) and 0.5 μl (5 U/μl) BioTaq DNA polymerase (Bioline, London) and amplified using PCR thermal cycler (BiometraTOne ...
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bioRxiv - Genetics 2022Quote: ... Genotyping was performed directly from the lysates with region targeting primers (Supplementary Table 2) and MyTaq Red Mix (Bioline) followed by gel electrophoresis to reveal biallelic knockout clones ...
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bioRxiv - Plant Biology 2023Quote: ... according to the manufacturer’s instructions and first strand cDNA synthesis was performed with oligo dT primers using BioScript reverse transcriptase (Bioline). GdMYBSG6 sequences have been deposited in GenBank under the following accession numbers ...
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bioRxiv - Cancer Biology 2023Quote: ... 0.25uM forward and reverse oligo primers (see supplementary table 6) and SensiFAST SYBR Hi-ROX Kit (Bioline, BIO-92005) in 384-well plates with the LightCycler 480 Instrument II (Roche ...
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bioRxiv - Molecular Biology 2024Quote: ... cDNA was synthesized utilizing a gene-specific primer designed to target the 10xSunTag socRNA and Tetro Reverse Transcriptase (Bioline). The resulting cDNA was isolated via column-based purification using the GeneJet Gel Extraction Kit (Thermo Scientific) ...
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bioRxiv - Neuroscience 2021Quote: Genomic DNA extraction from tail snips and genotyping PCR reactions were performed using MyTaq Extract-PCR kit (Bioline, Taunton, MA). All the mice were genotyped by the fragment PCR method as indicated in the Jackson Laboratory website ...
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bioRxiv - Molecular Biology 2020Quote: ... cDNA products were used as the template for quantitative reverse transcription-PCR (qRT-PCR) using the SensiMix SYBR No-ROX kit (Bioline). For relative quantification of gene expression ...
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bioRxiv - Microbiology 2019Quote: ... Reverse transcription and quantitative real-time PCRs (qRT-PCR) were performed using the SensiFast SYBR No-ROX One Step kit (Bioline) in a Rotor-Gene Q Lightcycler (Qiagen) ...
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bioRxiv - Microbiology 2020Quote: ... Influenza virus M gene copy number per μL RNA was determined by qRT-PCR using the SensiFAST Probe Lo-ROX One-Step qRT-PCR System Kit (Bioline) on the ABI 7500 Real Time PCR System (Applied Biosystems ...
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bioRxiv - Microbiology 2021Quote: ... Quantitative real time-PCR (qRT-PCR) analysis was performed in triplicate on 50 ng cDNA using the SensiFAST SYBR & Fluorescein kit (Bioline) and a Roche Lightcycler 96 ...
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bioRxiv - Physiology 2020Quote: ... Genomic DNA extraction from tails snips and genotyping PCR reactions were performed using MyTaq Extract-PCR kit (Bioline, Taunton, MA). Mice were genotyped using a fragment PCR method with the primers shown in the table below and indicated on the Jackson Laboratory website ...
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bioRxiv - Microbiology 2020Quote: ... PCR products were generated in 25 µl reactions containing 12.5 µl Bioline PCR Master Mix (Bioline USA Inc, Taunton, MA), 10.0 µl MG H2O ...
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bioRxiv - Microbiology 2023Quote: ... Reverse transcription and quantitative real-time PCRs (qRT-PCR) were performed using the SensiFast SYBR No-ROX One Step kit (Bioline) in a Rotor-Gene Q lightcycler (Qiagen) ...
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bioRxiv - Genomics 2023Quote: ... 1 μg of the entry vector was also digested with EcoRI-HF and NheI-HF and the linearised product was purified from a 2% agarose gel using PCR Isolate II PCR and Gel Kit (Bioline). The digested and purified reporter pool was then ligated into 80 ng of the linearised entry vector using Takara ligation kit v1.0 (#6021 ...
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bioRxiv - Microbiology 2023Quote: ... If a band was visualised at ∼480 bp the PCR product was purified using an ISOLATE II PCR and Gel Kit (Bioline).
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bioRxiv - Microbiology 2023Quote: ... PCR products that produced bands of the correct size were purified using the ISOLATE II PCR and Gel Kit (Bioline) as per manufacturer’s protocol and submitted for sequencing using an Applied Biosystems 3730xl capillary analyser (Macrogen) ...
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bioRxiv - Plant Biology 2024Quote: ... Quantitative Real-time PCR (qRT-PCR) was carried out using SensiFAST™ SYBR® No-ROX One-Step Kit (Bioline) and the results were analysed using the 2−ΔΔCt method (Livak & Schmittgen ...
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bioRxiv - Developmental Biology 2019Quote: ... PCR reactions were resolved in 1 % agarose (Bioline) gels containing 0.5 μg/ml Ethidium Bromide (Sigma ...
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bioRxiv - Cancer Biology 2020Quote: ... PCRs were conducted using SensiFast (Bioline, London, UK) on the CFX384 RT-PCR detection system (Bio-Rad) ...
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bioRxiv - Cell Biology 2022Quote: ... PCR machine using the SensiMix SYBR kit (Bioline). Results were quantified using the 2−ΔΔCt method ...
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bioRxiv - Physiology 2020Quote: ... PCR was performed with Biomix red kit (Bioline) and PCR products were resolved using the QIAxcelcapillary system (QIAGEN ...
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bioRxiv - Immunology 2020Quote: ... PCR was performed using a BIOTAQ kit (Bioline). Csf1rMeriCreMer mice were genotyped using iCre-F-YL (5’- GTCTCCAACCTGCTGACTGTGC-3’ ...
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bioRxiv - Molecular Biology 2024Quote: ... utilizing SensiFast Lo-ROX qRT-PCR Mastermix (Bioline) in both biological and technical triplicate ...
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bioRxiv - Developmental Biology 2022Quote: ... qPCR reactions were carried out using 1.25 ng total RNA equivalent RT reaction in sensiFAST SYBR No-Rox mix (Bioline) in presence of 600 nM of each primer ...
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bioRxiv - Biochemistry 2021Quote: ... RT-qPCR was carried out using SensiMix™ SYBR® and Fluorescein kit (QT615-05; Bioline, London, United Kingdom). The following primers were used ...
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bioRxiv - Neuroscience 2022Quote: ... RT-qPCR was performed with 1/10 diluted cDNA with the SensiFAST SYBR no-rox kit (Bioline, London, UK) and Lightcycler® 480 (Roche Molecular Biochemicals ...
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bioRxiv - Developmental Biology 2021Quote: ... PCR was done using gene-specific primers (see Supplemental Table 1) in technical triplicates on a LightCycler 480 system using the Sensifast SYBR Master mix (Bioline). The ratio of experimental target mRNA to an ACTIN control for each sample was calculated by Applied Biosystems software ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Quantitative PCR was carried out using CTS gene specific primer pairs (Supplemental Data 8) with SYBR green-based visualization of product amplification (Bioline). Changes in CTS gene transcript levels relative to Actin (ACT1 ...
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bioRxiv - Cancer Biology 2021Quote: ... and qPCR reactions were conducted using gene-specific primers with SensiFAST SYBR No-ROX kit (Bioline, #Cat no: BIO-98005) as described previously (Sibai et al. ...
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bioRxiv - Genetics 2019Quote: ... We tested the primers on DNA extracted from the liver of Mongolian gerbil and fat sandrat using the MyTaq Red Mix (Bioline). In each 25ul PCR reaction ...
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bioRxiv - Plant Biology 2020Quote: ... PCR was done using gene-specific primers in technical triplicates on a LightCycler 480 system using the Sensifast SYBR Master mix (Bioline). The ratio of experimental target mRNA to Actin control for each sample was calculated by Applied Biosystems software ...
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bioRxiv - Cell Biology 2020Quote: Real-Time PCR analysis was performed using TaqMan gene expression assays using primers (see Resources Table) and SensiFast Probe Lo-ROX Kit (cat.# BIO-84020, Bioline) following manufacturer’s recommendation ...
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bioRxiv - Genetics 2022Quote: ... 1 μg of RNA was then reverse transcribed with random hexamer and anchored oligo dT primers using SensiFast cDNA synthesis kit (Bioline). Quantitative PCR was performed on the diluted cDNA using SensiFast probe (Bioline ...
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bioRxiv - Molecular Biology 2022Quote: ... ChIP DNA and input DNA were diluted 5X and amplified using 500 nM primers and SensiFAST SYBR (No-ROX Kit, Bioline) using a LightCycler 480 (Roche).
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bioRxiv - Cancer Biology 2023Quote: ... 1 μl of the dilution was mixed with 0.41 μM primers (Supplemental Table S4) and 1x Sensifast SYBR non-Rox kit (Bioline Bio98005). The real-time PCR program cycled as follows ...
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bioRxiv - Genomics 2023Quote: ... final libraries were quantified by qPCR comparison to previously-sequenced NGS libraries using Illumina P5/P7 qPCR primers and SensiMix SYBR (Bioline).
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bioRxiv - Cell Biology 2020Quote: ... PCR bands were extracted from the agarose gel and purified using and Isolate II PCR and gel kit (Bioline, London, UK) and sent for Sanger sequencing (GATC Biotech ...
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bioRxiv - Microbiology 2022Quote: ... A few microliters of each PCR product were run on an agarose gel to assess the success of the PCR reaction and the remains cleaned through an Isolate II PCR and Gel kit (Bioline, USA) and sent for sequencing with primer C1-N-2191 ...
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bioRxiv - Microbiology 2021Quote: ... For the PCR master mix (MM) MangoTaq DNA polymerase and MyTaq DNA Polymerase were used and all PCR reagents were from Bioline (London, UK). To reduce the PCR inhibitors 0.4 μg μL−1 of bovine serum albumin (BSA ...
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bioRxiv - Molecular Biology 2020Quote: ... For RT-qPCR cDNA was prepared from 1 µg of total RNA using the SensiFAST cDNA Synthesis Kit (BIO-65054, Bioline) and analysed by qPCR using the SensiFast SYBR Lo-ROX Kit (BIO-94050 ...
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bioRxiv - Plant Biology 2020Quote: ... PCR was performed with BIOTAQ DNA Polymerase (Bioline, UK) using the following program ...
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bioRxiv - Molecular Biology 2020Quote: ... PCR of cDNA was performed using MyFi Taq (Bioline) following the manufacturer’s instructions ...