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Citations for Bioline :
151 - 200 of 292 citations for Recombinant Human Programmed Cell Death 1 Ligand 2 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: ... coli α-select chemically competent cells (Bioline BIO-85026) and colonies were selected on YT glucose plus 50 mg/mL spectinomycin HCl (Sigma-Aldrich S9007) ...
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bioRxiv - Molecular Biology 2021Quote: ... after which α-Select Bronze Efficiency Competent Cells (Bioline) were transformed with the ligation products ...
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bioRxiv - Developmental Biology 2019Quote: ... PCR reactions were resolved in 1 % agarose (Bioline) gels containing 0.5 μg/ml Ethidium Bromide (Sigma ...
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bioRxiv - Cancer Biology 2020Quote: ... DNA weight marker Hyperladder™ 1-kb (BIoline) and UltraRanger 1kb (Norgen Biotek ...
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bioRxiv - Genetics 2023Quote: ... Hyperladder 50 bp or Hyperladder 1 kb (Bioline) served as DNA fragment size markers.
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bioRxiv - Genomics 2023Quote: ... 1’ 72°C) by MyTaq Red mix (Bioline) using primers that add overhangs with EcoRI (MT024 ...
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bioRxiv - Immunology 2023Quote: ... The total qPCR reaction volume was 1.5 μl and consisted of 0.5 μl of cDNA (dilution 1/12) and 1 μl of SensiFAST™ SYBR® No-ROX Kit (Bioline) containing 0.4 μM of PCR primer (Eurogenetec SA)(primer list in sup ...
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bioRxiv - Cancer Biology 2020Quote: ... total RNA was extracted from cells using TriSure buffer (Bioline) followed by phenol/chloroform extraction and 1 μg of RNA was reverse transcribed with Quantitect RT kit (Qiagen ...
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bioRxiv - Immunology 2019Quote: ... Total RNA was extracted from cells using TriSure reagent (Bioline), with first strand cDNA being synthesized from total RNA and reverse transcribed using a Tetro cDNA synthesis kit (Bioline) ...
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bioRxiv - Microbiology 2019Quote: ... coli (alpha-select competent cells, Bioline or XL1-Blue, Stratagene) and plasmids were purified using Zyppy Plasmid Kits (Zymo Research Corporation) ...
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bioRxiv - Microbiology 2020Quote: ... Total RNA was extracted from cells using TriSure reagent (Bioline), with first strand cDNA being synthesized from total RNA and reverse transcribed using a Tetro cDNA synthesis kit (Bioline) ...
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bioRxiv - Microbiology 2021Quote: ... braarudii cells using the Isolate II RNA Mini Kit (Bioline) with on column DNA digestion ...
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bioRxiv - Molecular Biology 2023Quote: Total RNA was extracted from the cells using TRIsure (Bioline) following the manufacturer’s instruction ...
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bioRxiv - Molecular Biology 2024Quote: ... RNA from siRNA-treated cells was isolated using TRIsure (Bioline). Next ...
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bioRxiv - Microbiology 2019Quote: ... A second round of amplification was performed by transferring 5μl of 1:100 diluted Round 1 product into 15μl Bioline MyFi™ Mix (BIOLINE GmbH, Luckenwalde, Germany) reactions.
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bioRxiv - Immunology 2023Quote: ... the aqueous phase was mixed 1:1 v/v with 70% ethanol and loaded into a column of the Isolate II RNA Mini kit (Bioline, Cincinnati, OH). Total RNA was purified following the manufacturer’s recommendations ...
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bioRxiv - Molecular Biology 2021Quote: ... The final volume contained 1× SensiMix (Bioline, London, UK), 800 nM of each primer and 200nM of each probe and the PCR cycling conditions included an initial denaturation at 95°C for 10 minutes ...
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bioRxiv - Genomics 2023Quote: ... Samples were compared to 1 Kb hype ladder (Bioline). The primer sequences are listed in Table S9.
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bioRxiv - Cell Biology 2023Quote: ... and 1 unit of Taq polymerase (Bioline, Meridian Bioscience). The PCR conditions were an initial melting step of 94°C for 4 min ...
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bioRxiv - Molecular Biology 2020Quote: ... coli as per manufacturer’s protocol (α-Select Competent Cells, Bioline, Australia). The transformed cells were grown overnight (37° ...
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bioRxiv - Plant Biology 2019Quote: ... and transformed into Escherichia coli α-select chemically competent cells (Bioline) via heat shock ...
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Increased ACTL6A Occupancy Within mSWI/SNF Chromatin Remodelers Drives Human Squamous Cell CarcinomabioRxiv - Molecular Biology 2021Quote: ... cells were lysed directly on culture plates with TRIsure reagents (Bioline), and RNA was extracted using Direct-zol RNA MiniPrep kits (Zymo Research ...
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bioRxiv - Cancer Biology 2019Quote: Cells were lysed in TRIsure reagent (#BIO-38032, Bioline, London, UK), and RNA was extracted according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2021Quote: ... The amplified DNA was transformed into α-select competent cells (Bioline) by heat shock and plated on Luria-Bertani broth (LB ...
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bioRxiv - Microbiology 2019Quote: ... The 1 kb molecular weight marker (HyperLadder IV, Bioline, UK) was used in together with the amplified products ...
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bioRxiv - Microbiology 2020Quote: ... for 1 minute in 0.2 mL TRIsure (Bioline: BIO-38033). Homogenized tissue was brought to 1 mL volume with 0.8 mL TRIsure ...
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bioRxiv - Genetics 2020Quote: ... and 1 Unit MyTaqTM (Bioline; Meridian Bioscience Inc., London, UK) by incubation for 2 min at 96°C ...
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bioRxiv - Genomics 2023Quote: ... supplemented with 1:100 10 mg/mL Proteinase K (Bioline). TIDE was performed as described before (40) ...
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bioRxiv - Molecular Biology 2022Quote: Cells or frozen tissue samples were homogenised and lysed in TRIsure (Bioline). Total RNA was isolated using EconoSpin All-In-One Mini Spin Columns (EconoSpin 1920-250 ...
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bioRxiv - Biochemistry 2022Quote: ... Protein overexpression was performed using Escherichia coli BL21 (DE3) pLysS cells (BIOLINE) in super broth supplemented with 200 μg/mL ampicillin (AMRESCO ...
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bioRxiv - Cancer Biology 2022Quote: Cells were plated at 1M/mL and harvested in TRIsure (Bioline #38033). RNA was extracted using Direct-zol RNA MicroPrep columns (Zymo #R2062 ...
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bioRxiv - Genomics 2023Quote: ... cells were harvested and resuspended in 800 μl TRIsure (#BIO-38032; Bioline) and stored at -80 °C until further use ...
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bioRxiv - Cancer Biology 2023Quote: Cells were plated at 1M/mL and harvested in TRIsure (Bioline #38033). RNA was extracted using Direct-zol RNA MicroPrep columns (Zymo #R2062 ...
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bioRxiv - Microbiology 2021Quote: ... using 5 μl product with 1 μl 5X loading dye (BioLine) to verify bands of approximately 450 bp ...
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bioRxiv - Synthetic Biology 2023Quote: ... Molecular weight markers used were the 1 kb HyperLadder (from Bioline). T4 DNA ligase and buffer were purchased from New England Biolabs ...
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bioRxiv - Molecular Biology 2023Quote: ... The resultant bands were compared to a 1 Kb HyperLadder (Bioline) using the expected sizes per gene region as follows ...
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bioRxiv - Cancer Biology 2021Quote: ... RNA was extracted from cultured cells using ISOLATE II RNA Mini Kit (Bioline) and used as a template to generate cDNA with Tetro cDNA Synthesis Kit (Bioline) ...
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bioRxiv - Microbiology 2021Quote: Total RNA was isolated from cells with Isolate II RNA Mini Kit (Bioline) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: RNA was isolated from cells using the ISOLATE II RNA Mini Kit (Bioline) and concentration was measured by Nanodrop (Thermo Scientific) ...
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bioRxiv - Molecular Biology 2020Quote: ... buffer with 1 mg/ml Proteinase K (Bioline, Cat. No. BIO-37084) to pre-lyse the cells ...
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bioRxiv - Microbiology 2019Quote: ... A 1 kilobase molecular-weight size marker (Hyperladder 1KB from Bioline UK) was used to confirm that the 16S ribosomal RNA hypervariable region had been isolated (supplementary material) ...
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bioRxiv - Microbiology 2021Quote: ... 1% SDS (Sigma-Aldrich)] supplemented with 5 μL Proteinase K (20 mg mL−1; Bioline Australia) to lyse cells ...
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bioRxiv - Molecular Biology 2022Quote: ... supplemented with 1 mg/ml proteinase K (Bioline, cat. no. BIO-37084) by incubating them at 55 °C for at least 2 hours up to overnight ...
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bioRxiv - Biochemistry 2023Quote: ... RNA was extracted from 293T cells using the Isolate II RNA mini kit (Bioline) and cDNA synthesised using oligo(dT ...
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bioRxiv - Molecular Biology 2024Quote: ... RNA was isolated from cells 3 hours after inducing socRNA expression using TRIsure (Bioline). Subsequently ...
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bioRxiv - Molecular Biology 2020Quote: ... or HyperLadder 1 kb (range 200 bp to 10 kb, BIO-33053, Bioline) to determine product sizes.
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bioRxiv - Plant Biology 2020Quote: ... The PCR product was visualized on a 1% (w/v) agarose gel (Bioline) by electrophoresis at 90 V for 1 h and the 1.5 kb target band was collected for purification using NucleoSpin® Gel and PCR Clean-up kit (Macherey-Nagel ...
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bioRxiv - Biochemistry 2022Quote: ... RNA samples (1 µg) were reverse transcribed using SensiFAST cDNA synthesis kit (Bioline) according to manufacturer’s protocol and subjected to Real-time quantitative PCR (RT-qPCR ...
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bioRxiv - Cell Biology 2020Quote: Total RNA was extracted from cell pellets using the Isolate II RNA Mini Kit (Bioline) RNA extraction Kit following manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: DNA was isolated from cells using the Isolate II Genomic DNA kit (Bioline, NSW, Australia) following the manufacturer’s protocol ...