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Citations for Bioline :
51 - 100 of 105 citations for Recombinant E. coli LT IIc His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2019Quote: ... and transformed into Escherichia coli α-select chemically competent cells (Bioline) via heat shock ...
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bioRxiv - Molecular Biology 2023Quote: ... coli (TOP10- or Stbl3) using Isolate II plasmid miniprep kit (Bioline).
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bioRxiv - Microbiology 2022Quote: ... utilizing the SensiMix™ SYBR® Hi-ROX Kit (Bioline, Meridian Bioscience; Cincinnati, OH), as previously described (primers ...
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bioRxiv - Molecular Biology 2020Quote: ... 1.6ul reverse primer (5uM) and 10ul SensiFAST SYBR Hi-ROX mix (Bioline, Cat: BIO92020). The plate was then loaded into an Agilent AriaMax real time PCR machine (Agilent Technologies ...
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bioRxiv - Evolutionary Biology 2019Quote: ... using the SensiFAST™ Sybr® Hi-ROX Kit (#BIO-92020; Bioline, London, UK). Amplification was performed in a reaction volume of 10 μL containing 500 nM of each of the forward and reverse primers and 4 μL of a two-fold dilution of C ...
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bioRxiv - Cell Biology 2020Quote: ... subsequently diluted in water (1:4) and mixed with SensiFAST SYBR Hi-Rox (Bioline) and the appropriate primers at a final concentration of 10 μM ...
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Isonicotinamide extends yeast chronological lifespan through a mechanism that diminishes nucleotidesbioRxiv - Biochemistry 2021Quote: ... and quantitated by real-time PCR reactions using SensiMix SYBR Hi-ROX Mastermix (Bioline) run on a StepOne Plus thermocycler (Applied Biosystems) ...
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bioRxiv - Genomics 2022Quote: ... Quantification was carried using SensiFAST™ SYBR® Hi-ROX One-Step Kit (Bioline). Absolute RNA quantification was obtained by interpolating its CT value against the standard curve ...
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bioRxiv - Microbiology 2020Quote: ... coli were extracted using the ISOLATE II Plasmid Mini kit (Bioline, Australia) according to manufacturer’s instruction ...
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bioRxiv - Biochemistry 2022Quote: ... Protein overexpression was performed using Escherichia coli BL21 (DE3) pLysS cells (BIOLINE) in super broth supplemented with 200 μg/mL ampicillin (AMRESCO ...
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bioRxiv - Cancer Biology 2021Quote: ... The RT-qPCR reaction was performed using SensiFAST SYBR Hi-ROX Kit (Bioline #BIO-92020) on the StepOnePlus™ platform (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2021Quote: ... Real-time PCR was carried out using the SensiFAST SYBR Hi-ROX kit (Bioline, BIO92005). Briefly ...
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bioRxiv - Immunology 2022Quote: ... Quantitative PCR were performed on 20ng cDNA using and SensiFAST Probe Hi-ROX kits (BioLine) on a Quant Studio III (Applied BioSystem) ...
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bioRxiv - Neuroscience 2023Quote: Human samples: qPCR experiments were conducted using the SensiFAST Probe Hi-ROX Master Mix (Bioline) and the TaqMan Gene Expression Assays (Applied Biosystems ...
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bioRxiv - Microbiology 2020Quote: ... prat2 expression was assessed with the SensiFAST™ SYBR® Hi-ROX One-Step Kit (Bioline) according to manufacturer’s recommendations with specific primers PP25361 (Forward ...
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bioRxiv - Biochemistry 2020Quote: ... using TaqMan™ Gene Expression Assays (FAM-labelled) and the SensiFASTTM Probe Hi-ROX kit (Bioline). ΔCt was calculated as Ct [Target]-Geometric mean (Ct [HPRT1] ...
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bioRxiv - Cancer Biology 2019Quote: ... Quantitative-RT PCR was then performed using the SensiFAST SYBR Green Hi-ROX master mix (Bioline). Primers were designed (Sigma Aldrich ...
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bioRxiv - Evolutionary Biology 2022Quote: ... qPCR was performed on an Applied Biosystems StepOnePlus system using Sensifast Hi-Rox Sybr kit (Bioline) with the following PCR cycle ...
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bioRxiv - Biochemistry 2023Quote: ... using TaqMan™ Gene Expression Assays (FAM-labelled) and the SensiFASTTM Probe Hi-ROX kit (Bioline). The following TaqMan™ Gene Expression Assays were used ...
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bioRxiv - Cell Biology 2021Quote: ... and E-gene expression assessed using the SensiFAST Probe No-Rox One Step Kit (Bioline) and the following primers/probes ...
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bioRxiv - Bioengineering 2022Quote: ... E-gene expression was determined using the SensiFAST Probe No-Rox One Step Kit (Bioline) and the following primers/probes ...
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bioRxiv - Evolutionary Biology 2021Quote: ... qRT-PCR was performed on an Applied Biosystems StepOnePlus system using Sensifast Hi-Rox Sybr kit (Bioline) with the following PCR cycle ...
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bioRxiv - Cell Biology 2021Quote: ... RT-qPCR was performed using SensiFAST™ SYBR® Hi-ROX Kit (Bioline, cat. no. BIO-92020) in a 96-well white plate (Roche ...
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bioRxiv - Evolutionary Biology 2022Quote: ... qRT–PCR was performed on an Applied Biosystems StepOnePlus system using Sensifast Hi-Rox Sybr kit (Bioline) with 1 µl of complementary DNA (cDNA ...
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bioRxiv - Evolutionary Biology 2023Quote: ... qRT-PCR was carried out on 1:10 diluted cDNA using Sensifast Hi-Rox Sybr kit (Bioline). Two qRT-PCR reactions (technical replicates ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Quantitative analysis of the ERBB2 was performed using a SensiFAST™ SYBR Hi-ROX kit (Bioline, Canada). PCR amplification condition was applied as described previously [29] ...
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bioRxiv - Molecular Biology 2021Quote: ... qPCR quantification of DNA samples was carried out using the SensiFAST SYBR Hi-ROX Kit (Bioline, BIO-92005) in 96-well plates using a ABI PRISM 7000 system (Applied Biosystems) ...
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bioRxiv - Molecular Biology 2019Quote: ... Real-time PCRs were analyzed by SensiFAST™ SYBR® Hi-ROX Kit (BIO-92020, Bioline, London, UK). A PCR program was used as described ...
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bioRxiv - Microbiology 2021Quote: ... 1:10 dilutions of RNA samples were analyzed using SensiFast SYBR Hi-ROX One-Step kit (Bioline, UK) and 400 nM primer concentrations ...
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bioRxiv - Developmental Biology 2019Quote: ... 2.5 ng cDNA was amplified in a 10 μl reaction containing 1x Sensimix Sybr Hi-Rox reagents (Bioline) and 375 nM of both forward and reverse primer (Supplementary Table S1) ...
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bioRxiv - Developmental Biology 2019Quote: ... 20 ng DNA was used in a 10 μl reaction containing 1x Sensimix Sybr Hi-Rox reagents (Bioline) and 375 nM of both forward and reverse primer (Supplementary Table S1) ...
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bioRxiv - Genetics 2022Quote: The relative amount of PCR product was quantified by qPCR using SensiFASTTM SYBR® Hi -ROX Kit (Bioline). The sequences of primers used for the quantitative detection of the chromosomal loci are listed in Suppl Table ST6 ...
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bioRxiv - Physiology 2022Quote: ... qPCRs were carried out in 15 μl in a 384-well plate using SensiFASTTM SYBR Hi-ROX kit (Bioline), 0·5 μM of each forward and reverse primer and 2·5 μl of template DNA ...
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bioRxiv - Cancer Biology 2023Quote: ... 0.25uM forward and reverse oligo primers (see supplementary table 6) and SensiFAST SYBR Hi-ROX Kit (Bioline, BIO-92005) in 384-well plates with the LightCycler 480 Instrument II (Roche ...
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bioRxiv - Neuroscience 2023Quote: ... Real-time RT-PCR was performed using a SensiFastTM SYBR® Hi-ROX kit (#BIO-92020; Bioline USA Inc.) in an Applied Biosystems QuantStudio 7 Flex Real-Time PCR system (Thermo Fisher Scientific Inc.) ...
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bioRxiv - Bioengineering 2019Quote: ... coli DH5α was used as the cloning strain and performed transformations according to the manufacturer’s instructions (BIOLINE). E ...
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bioRxiv - Evolutionary Biology 2020Quote: ... We labeled each library with a sample-specific pair of barcodes and combined libraries in equimolar ratios based on qPCR using SensiFAST SYBR Hi-ROX master mix (Bioline), then sequenced the combined pools on four lanes of 50bp single end reads (Illumina HiSeq2500 ...
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bioRxiv - Evolutionary Biology 2021Quote: ... qRT-PCR was carried out on 1:10 diluted cDNA on an Applied Biosystems StepOnePlus system using Sensifast Hi-Rox Sybr kit (Bioline). Cycle conditions were as follows ...
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bioRxiv - Neuroscience 2021Quote: ... cDNA samples were run in triplicate in 96 well reaction plates using SYBR-Green-based qPCR (SensiFast, HI-ROX, Bioline) according to manufacturer’s instructions using a StepOnePlus System (Applied Biosystems ...
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bioRxiv - Neuroscience 2020Quote: ... The cDNA products were diluted 10 times and used for qPCR using a StepOnePlusTM instrument (Applied Biosystem, USA) and SensiFAST SYBR Hi-ROX Kit (Bioline) following the manufacturer’s guidelines ...
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bioRxiv - Microbiology 2021Quote: qRT-PCR was used to quantify transcript levels of specific quorum sensing-controlled genes in DS40M4 and was performed using the SensiFast SYBR Hi-ROX One-Step Kit (Bioline) according to the manufacturer’s guidelines ...
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bioRxiv - Microbiology 2021Quote: Real-time qRT-PCR (quantitative reverse transcription PCR) was used to quantify transcript levels using the SensiFast SYBR Hi-ROX One-Step Kit (Bioline) according to the manufacturer’s guidelines ...
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bioRxiv - Neuroscience 2021Quote: ... The cDNA was then diluted and used as template for qPCR, which was performed with a StepOnePlus instrument (Applied Biosystem, USA) and SensiFAST SYBR Hi-ROX Kit (Bioline) according to the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2022Quote: qPCR was performed on each of the samples using an Applied Biosystems StepOnePlus system with a Sensifast Hi-Rox Sybr kit (Bioline). Cycle conditions were as follows ...
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bioRxiv - Evolutionary Biology 2022Quote: ... qRT-PCR was carried out on 1:2 diluted cDNA on an Applied Biosystems StepOnePlus system using a Sensifast Hi-Rox SYBR kit (Bioline). Cycle conditions were as follows ...
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bioRxiv - Cell Biology 2020Quote: ... PDZ4 (1099–1203)) as well as Scribble mutants were expressed using Escherichia coli BL21 (DE3) pLysS cells (BIOLINE) as Glutathione S-transferase or Maltose Binding Protein fusions (Rautureau et al. ...
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bioRxiv - Neuroscience 2019Quote: ... The cDNA products were then diluted 10 times and applied for qPCR using a StepOnePlus™ instrument (Applied Biosystem, USA) and SensiFAST SYBR Hi-ROX Kit (Bioline) followed the protocol from the manufacturer ...
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bioRxiv - Plant Biology 2020Quote: ... Quantitative real-time RT–PCR (qRT–PCR) was carried out using the SensiFast™SYBR Hi-ROX One-Step Kit (Bioline) as described (Wobbe et al. ...
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bioRxiv - Microbiology 2023Quote: PCR products for the generation of different potential RNase E targets were amplified with MyTaq™ Red Mix 2x (Bioline), and Synechocystis genomic DNA as template (primers T01 to T12) ...
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bioRxiv - Cell Biology 2023Quote: ... RNA was isolated from lung homogenates from mice exposed to E-cigarettes (with and without nicotine) and room air (SHAM) using Isolate II RNA Mini Kit (Bioline) and cDNA was synthesized using SensiFAST cDNA Synthesis kit (Bioline) ...