Labshake search
Citations for Bioline :
501 - 550 of 716 citations for Rat Protein SET SET ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... PCR products were purified using the Bioline Isolate II PCR and Gel kit (Bioline, Cat. No. BIO-52060). Samples were sequenced using the Illumina Miseq platform ...
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bioRxiv - Molecular Biology 2019Quote: ... Real-time PCRs were analyzed by SensiFAST™ SYBR® Hi-ROX Kit (BIO-92020, Bioline, London, UK). A PCR program was used as described ...
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bioRxiv - Cell Biology 2020Quote: ... cDNA was retrotranscribed from 0.3 µg to 1 µg using the SensiFAST cDNA Synthesis Kit (Bioline, BIO-65054). Real-time PCR was performed on the CFX384 Touch Real-time PCR Detection System (Bio-Rad ...
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bioRxiv - Plant Biology 2020Quote: ... RNA was used for reverse transcription according to the protocol of the SensiFASTtm cDNA synthesis kit from Bioline. PCR reactions were carried out with 5 μL of 100x diluted cDNA solution ...
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bioRxiv - Microbiology 2020Quote: ... cDNA was synthesised using SuperScript III reverse transcriptase from RNA extracted using ISOLATE II RNA Mini Kit (Bioline) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... Bands of correct size were cut out and purified using the Isolate II PCR and gel kit (Bioline). 50 ng of vector and 150 ng of each fragment were mixed with 3 μl of HiFi DNA Master Mix (NEB ...
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bioRxiv - Cancer Biology 2021Quote: ... The conversion of messenger RNA into cDNA was performed using the Sensifast cDNA synthesis kit (Bioline, BIO- 65053). cDNA was then amplified by PCR using specific primers for each gene designed with Primer-blast software (https://www.ncbi.nlm.nih.gov/tools/primer-blast/ ...
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bioRxiv - Genetics 2021Quote: ... 1 µg of total RNA was reverse transcribed using SensiFAST cDNA Synthesis Kit following the manufacturer’s protocol (Bioline).
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bioRxiv - Plant Biology 2021Quote: ... Reverse transcription was performed in 20mL with 1mg of total RNA using the Tetro cDNA synthesis kit (Bioline). RT-negative (no enzyme ...
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bioRxiv - Microbiology 2021Quote: ... 1:10 dilutions of RNA samples were analyzed using SensiFast SYBR Hi-ROX One-Step kit (Bioline, UK) and 400 nM primer concentrations ...
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bioRxiv - Microbiology 2021Quote: ... Two micrograms of total RNA was reverse-transcribed with Sensi-FAST™ cDNA Synthesis Kit (Bioline, Memphis, USA). The amounts of p16 ...
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bioRxiv - Molecular Biology 2019Quote: DNA was extracted from surface-sterilized whole insects using the ISOLATE II Genomic DNA Kit (Bioline, London, UK) according to the manufacturer’s instructions ...
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bioRxiv - Pathology 2019Quote: ... PCR amplicons of each sample were pulled together and purified using Isolate II PCR and gel Kit (BIOLINE) and about 100 ng of DNA was used in library preparation ...
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bioRxiv - Evolutionary Biology 2020Quote: ... DNA from historical museum toepad samples was extracted using the Bioline Isolate II Genomic DNA extraction kit (Bioline), but suspending tissues in 400 μL lysis buffer with 25 μL proteinase k and incubated overnight at 55 °C ...
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bioRxiv - Microbiology 2021Quote: ... Amplicons of appropriate size were excised and purified using an Isolate II PCR and Gel kit (Bioline, UK). The purified amplicons were included in cycle sequence reactions ...
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bioRxiv - Immunology 2021Quote: ... Real time quantitative PCR (RT-qPCR) analysis was carried out using the SensiMix SYBR No-Rox kit (Bioline), using primers and conditions shown in Supplemental Table 1 ...
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bioRxiv - Microbiology 2022Quote: Faecal DNA was extracted from FecalSwabTM media (200µL) using the ISOLATE II Fecal DNA Kit (Bioline, Sydney, Australia) following the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2022Quote: Isolation of total RNA and cDNA synthesis was performed by using the Isolate II RNA Mini Kit (Bioline) and SensiFAST cDNA Synthesis Kit (Bioline) ...
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bioRxiv - Cell Biology 2022Quote: ... Real-time PCR analysis was performed according to the manufacturer’s protocol (SensiMix™ SYBR® & Fluorescein Kit, Bioline) with a MyiQ Single-Color Real-Time PCR detection system (Bio-Rad ...
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bioRxiv - Genetics 2022Quote: The relative amount of PCR product was quantified by qPCR using SensiFASTTM SYBR® Hi -ROX Kit (Bioline). The sequences of primers used for the quantitative detection of the chromosomal loci are listed in Suppl Table ST6 ...
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bioRxiv - Microbiology 2022Quote: ... conventional PCR assays were replaced with qPCR assays with SensiFAST™ SYBR® No-ROX Kit (Bioline, Australia). The cycling conditions included an initial denaturation at 95°C for 3 min followed by 28 -40 cycles of 95°C for 5 sec ...
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bioRxiv - Plant Biology 2023Quote: ... The total RNA was extracted from ground root samples using an ISOLATE II Plant Mini Kit (Bioline, Australia), cDNA was synthesised from 1ug of RNA using a SensiFAST cDNA Synthesis Kit (Bioline ...
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bioRxiv - Cancer Biology 2022Quote: ... transcripts were amplified directly on RNA samples using a one-step qRT-PCR kit with SYBR green (Bioline). Primers used against DDIT3 (CHOP ...
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bioRxiv - Cancer Biology 2022Quote: ... cDNA was prepared for RT-qPCR using the SensiFAST cDNA preparation kit according to manufacturer instructions (Bioline #65054). 1μL of cDNA was used per RT-qPCR reaction prepared with SYBR Lo-ROX (Bioline #94020) ...
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bioRxiv - Molecular Biology 2023Quote: ... RNA was reverse-transcribed (1 mg each experimental point) by using SensiFAST cDNA Synthesis Kit (BIO-65053, Bioline) and qPCR was performed as described (18 ...
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bioRxiv - Genetics 2023Quote: ... 1 µg of RNA was used to prepare cDNA using the Sensifast cDNA synthesis kit (Bioline, London, UK). 2 µl aliquots of cDNA (corresponding to 20 ng of cDNA ...
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bioRxiv - Plant Biology 2023Quote: Measurements were carried out in 96-well plates using the SensiFAST™ SYBR® Kit (Bioline, Luckenwalde, Germany) in a LightCycler® 480 (Roche) ...
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bioRxiv - Evolutionary Biology 2023Quote: ... primers at a final concentration of 800nM and 6μL of SensiFAST™ SYBR® Lo-ROX Kit (Bioline). qPCR conditions were ...
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bioRxiv - Evolutionary Biology 2023Quote: ... 600 ng of isolated RNA was reverse transcribed to complementary DNA (cDNA) with SensiFAST cDNA Synthesis -kit (Bioline) following the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... Genomic DNA was extracted from parasite lines using an Isolate II Genomic DNA Kit (Bioline, Meridian Bioscience Australia), following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... Correct sized amplicons were then purified using an Isolate II PCR and Gel Kit (Bioline, Meridian Biosciences, Australia) following the manufacturer’s instructions for PCR clean-up and sent for Sanger sequencing at the Victorian Clinical Genetics Services (VCGS ...
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bioRxiv - Molecular Biology 2023Quote: ... cDNA was prepared for RT-qPCR using the SensiFAST cDNA preparation kit according to manufacturer instructions (Bioline #65054). 1µL of cDNA was used per RT-qPCR reaction prepared with SYBR Lo-ROX (Bioline #94020) ...
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Mapping chromatin state and transcriptional response in CIC-DUX4 undifferentiated round cell sarcomabioRxiv - Cancer Biology 2023Quote: ... Total RNA (1000 ng) was used in a reverse transcriptase reaction with the SensiFAST cDNA Synthesis Kit (Bioline). Quantitative PCR included 3 replicates per cDNA sample ...
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bioRxiv - Plant Biology 2024Quote: ... This template was then used to quantify relative mRNA abundance using the SensiMix SYBR Low-ROX kit (Bioline), a LightCycler® 480 (Roche ...
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bioRxiv - Neuroscience 2024Quote: ... and qPCR performed in technical triplicate using SensiFAST™ SYBR® Lo-ROX Kit following manufacturer instructions (Bioline) on QuantStudioTM 5 Real-Time PCR system as previously described (34,35,37,41) ...
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bioRxiv - Microbiology 2020Quote: ... cells were collected by centrifugation at 4000 rpm for 15 minutes and pellets used for RNA extraction by RNeasy kit according to the manufacturer’s protocol (Bioline). cDNA synthesis and RT-qPCR was performed as previously described (60) ...
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bioRxiv - Cell Biology 2020Quote: ... Then 5 μg of the extracted RNA was reversely transcribed by utilizing Tetro cDNA Synthesis Kit (Bioline, BIO-65043) and random Hexamer Primer according to the manufacturer’s protocols ...
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bioRxiv - Genetics 2021Quote: ... RNA was reverse-transcribed (1 µg each experimental point) by using SensiFAST™ cDNA Synthesis Kit (BIO-65053, Bioline) and qPCR was performed using SensiFast Sybr Lo-Rox Mix (BIO-94020 ...
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bioRxiv - Developmental Biology 2021Quote: ... cDNA was made from 1 ug total RNA using Tetro cDNA synthesis kit with DNase treatment according to manufacturer’s instructions (Bioline). cDNA was diluted into 100 ul and 2 ul of cDNA was used to perform qRT-PCR ...
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bioRxiv - Zoology 2019Quote: ... The amplification was performed in a final volume of 12.5 μl using the SensiMix SYBR Low-ROX Kit (Bioline) with each primer at 200 nM ...
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bioRxiv - Plant Biology 2020Quote: ... 200 ng total RNA was used in each single-step reaction according to the manufacturer’s instruction (HiRoxSensifast Kit, Bioline). Primers (LHCSR3 fw/rev ...
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bioRxiv - Biochemistry 2021Quote: ... RT-qPCR was carried out using SensiMix™ SYBR® and Fluorescein kit (QT615-05; Bioline, London, United Kingdom). The following primers were used ...
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bioRxiv - Plant Biology 2020Quote: ... Real-time PCR reactions were performed in a 384-well microlitre using SensiFAST SYBR® No-ROX Kit (Bioline). The primers used here were designed using the open-source program QuantPrime-qPCR primer designed tool (Arvidsson et al. ...
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bioRxiv - Microbiology 2021Quote: ... After PCR verification in agarose gel and PCR clean-up using the ISOLATE II PCR and Gel Kit (Bioline), the remaining plasmid template was digested with DpnI at 37°C for 3h ...
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bioRxiv - Immunology 2022Quote: RNA was isolated from lung and blood using Isolate II RNA mini kit protocol (Bioline Reagents Ltd., London, UK) with slight modification ...
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bioRxiv - Molecular Biology 2020Quote: ... cDNA synthesis was carried out with 500 ng of RNA using the SensiFAST cDNA Synthesis Kit (Bioline, #BIO-65054) according to the manufacturer’s instructions.
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bioRxiv - Immunology 2019Quote: ... with first strand cDNA being synthesized from total RNA and reverse transcribed using a Tetro cDNA synthesis kit (Bioline). Quantitative real-time PCR was performed in a CFX Connect Real-Time Detection System (BioRad ...
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bioRxiv - Microbiology 2019Quote: ... with total reaction volume of 10 μl containing 5 μl Rotor-Gene SYBR® Green PCR Kit (Bioline, Australia), 0.3 μl each of 10 μM forward and reverse primer and 2 μl of genomic DNA ...
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bioRxiv - Plant Biology 2020Quote: ... First-strand cDNA was made from 2 μg of RNA using the Tetro cDNA synthesis kit with DNase treatment according to manufacturer’s instructions (Bioline). cDNA was diluted 1 to 5 into RNAse free water to a total of 100 μl ...
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bioRxiv - Microbiology 2021Quote: ... Genomic DNA was extracted from FecalSwab™ sample media (200μL) using the ISOLATE Fecal DNA kit (Bioline, Sydney, Australia) as per manufacturer’s instructions ...