Labshake search
Citations for Bioline :
151 - 200 of 947 citations for RT PCR kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
CRISPR-SID: identifying EZH2 as a druggable target for desmoid tumors via in vivo dependency mappingbioRxiv - Cancer Biology 2021Quote: ... Real-time quantitative PCR was performed using the SensiFAST™ SYBR® No-ROX Kit (Bioline) combined with a LightCycler® 480 Real-Time PCR System (Roche) ...
-
bioRxiv - Microbiology 2021Quote: qRT-PCR was performed using the SensiFAST Probe Lo-ROX One-Step kit (Bioline Meridian, Australia) with a reaction volume of 20µl ...
-
bioRxiv - Cell Biology 2021Quote: ... The cDNA (100 ng) was analysed by quantitative PCR using SensiFASTTM SYBR Lo-ROX Kit (Bioline) in the 7500 Fast Real-Time PCR System (Applied Biosystems) ...
-
bioRxiv - Synthetic Biology 2021Quote: ... We performed colony PCR using BioRed PCR mix (Bioline) to screen for genome modifications ...
-
bioRxiv - Evolutionary Biology 2021Quote: ... qRT-PCR was performed on an Applied Biosystems StepOnePlus system using Sensifast Hi-Rox Sybr kit (Bioline) with the following PCR cycle ...
-
bioRxiv - Plant Biology 2020Quote: ... Quantitative Real-Time PCR was performed using a SensiFAST™ SYBR® No-ROX Kit (Bioline; www.bioline.com). Fluorescence was monitored with a CFX384 thermal cycler (Bio-Rad) ...
-
bioRxiv - Plant Biology 2021Quote: ... qRT-PCR of protonema samples was conducted using the SensiFastTM SYBR No-ROX Kit (Bioline, Luckenwalde, Germany) in a LightCycler 480 (Roche ...
-
bioRxiv - Evolutionary Biology 2022Quote: ... qRT–PCR was performed on an Applied Biosystems StepOnePlus system using Sensifast Hi-Rox Sybr kit (Bioline) with 1 µl of complementary DNA (cDNA ...
-
bioRxiv - Molecular Biology 2022Quote: ... Real- time PCR was performed using 2x SensiMix SYBR and Fluorescein Kit (Bioline, QT615-05, Luckenwalde, Germany), 20ng cDNA and pre-tested gene-specific primer sets (listed in Supplementary table 3) ...
-
bioRxiv - Evolutionary Biology 2023Quote: ... qRT-PCR was carried out on 1:10 diluted cDNA using Sensifast Hi-Rox Sybr kit (Bioline). Two qRT-PCR reactions (technical replicates ...
-
bioRxiv - Molecular Biology 2019Quote: ... Real-time PCRs were analyzed by SensiFAST™ SYBR® Hi-ROX Kit (BIO-92020, Bioline, London, UK). A PCR program was used as described ...
-
bioRxiv - Cell Biology 2021Quote: ... Bands of correct size were cut out and purified using the Isolate II PCR and gel kit (Bioline). 50 ng of vector and 150 ng of each fragment were mixed with 3 μl of HiFi DNA Master Mix (NEB ...
-
bioRxiv - Microbiology 2021Quote: ... Amplicons of appropriate size were excised and purified using an Isolate II PCR and Gel kit (Bioline, UK). The purified amplicons were included in cycle sequence reactions ...
-
bioRxiv - Cell Biology 2022Quote: ... Real-time PCR analysis was performed according to the manufacturer’s protocol (SensiMix™ SYBR® & Fluorescein Kit, Bioline) with a MyiQ Single-Color Real-Time PCR detection system (Bio-Rad ...
-
bioRxiv - Genetics 2022Quote: The relative amount of PCR product was quantified by qPCR using SensiFASTTM SYBR® Hi -ROX Kit (Bioline). The sequences of primers used for the quantitative detection of the chromosomal loci are listed in Suppl Table ST6 ...
-
bioRxiv - Microbiology 2022Quote: ... conventional PCR assays were replaced with qPCR assays with SensiFAST™ SYBR® No-ROX Kit (Bioline, Australia). The cycling conditions included an initial denaturation at 95°C for 3 min followed by 28 -40 cycles of 95°C for 5 sec ...
-
bioRxiv - Cancer Biology 2022Quote: ... transcripts were amplified directly on RNA samples using a one-step qRT-PCR kit with SYBR green (Bioline). Primers used against DDIT3 (CHOP ...
-
bioRxiv - Molecular Biology 2023Quote: ... Correct sized amplicons were then purified using an Isolate II PCR and Gel Kit (Bioline, Meridian Biosciences, Australia) following the manufacturer’s instructions for PCR clean-up and sent for Sanger sequencing at the Victorian Clinical Genetics Services (VCGS ...
-
bioRxiv - Plant Biology 2020Quote: ... Real-time PCR reactions were performed in a 384-well microlitre using SensiFAST SYBR® No-ROX Kit (Bioline). The primers used here were designed using the open-source program QuantPrime-qPCR primer designed tool (Arvidsson et al. ...
-
bioRxiv - Microbiology 2019Quote: ... with total reaction volume of 10 μl containing 5 μl Rotor-Gene SYBR® Green PCR Kit (Bioline, Australia), 0.3 μl each of 10 μM forward and reverse primer and 2 μl of genomic DNA ...
-
bioRxiv - Cancer Biology 2021Quote: ... The relative amount of mRNA transcripts was measured by real-time PCR using SensiFAST SYBR N-ROX Kit (Bioline) and calculated by Bio-Rad CFX Manager Software ...
-
bioRxiv - Microbiology 2023Quote: ... The qRT-PCR reaction was performed using 35 μL SensiFAST™ Probe LoROX One-Step Kit (Bioline, Taunton, MA) and 15 μL of extracted eluate ...
-
bioRxiv - Microbiology 2023Quote: ... qRT-PCR analysis was performed in triplicate on 50 ng of cDNA using the SensiFAST SYBR & Fluorescein kit (Bioline) with a Roche Lightcycler 96 ...
-
bioRxiv - Genomics 2023Quote: ... the k13 and pfcrt loci was amplified directly from recrudesced infected blood using the MyTaq Blood-PCR Kit (Bioline) (68) ...
-
bioRxiv - Plant Biology 2023Quote: ... the extracts were reverse transcribed using random hexamers and Tetro RT enzyme (Bioline). The obtained cDNA was amplified with the MangoTaq™ DNA Polymerase and the primers described by Gaafar et al. ...
-
bioRxiv - Genomics 2024Quote: ... RT-qPCR was conducted using SensiMix SYBR Hi-ROX 2x Master Mix (Bioline) on the Rotor-Gene Q (Qiagen) ...
-
bioRxiv - Cell Biology 2020Quote: ... cDNA was used to analyse differences in gene expression by qRT-PCR employing SensiMix SYBR low-Rox KIT (Bioline, Germany) along with gene specific forward and reverse primers (250 nM ...
-
bioRxiv - Cell Biology 2020Quote: ... The relative amount of PAK transcript variants was measured by quantitative real-time PCR (qPCR) using SensiFAST SYBR No-ROX Kit (Bioline) and calculated using Bio-Rad CFX Manager Software ...
-
bioRxiv - Evolutionary Biology 2020Quote: ... Telomere length was measured using real-time quantitative PCR (qPCR) and a SensiMix SYBR No-ROX Kit (Bioline, Sydney, Australia). The telomere primers used were Tel1b (5’-CGGTTTGTTTGGGTTTGGGTTT GGGTTTGGGTTTGGGTT-3’ ...
-
bioRxiv - Plant Biology 2020Quote: ... Quantitative real-time PCR reactions using cDNA samples as template were carried out using a SensiFAST SYBR No-ROX Kit (Bioline) in a CFX96 Touch (BIO-RAD ...
-
bioRxiv - Neuroscience 2020Quote: ... Quantitative PCR (qPCR) was carried out using the cDNA generated previously employing the SensiFAST SYBR mix No-Rox Kit (Bioline, UK in a Rotor-Gene 6000 real-time PCR cycler (Corbett Research ...
-
bioRxiv - Microbiology 2021Quote: Real-time qRT-PCR (quantitative reverse transcription PCR) was used to quantify transcript levels using the SensiFast SYBR Hi-ROX One-Step Kit (Bioline) according to the manufacturer’s guidelines ...
-
bioRxiv - Microbiology 2020Quote: ... Detection and quantification of viruses was conducted by qRT-PCR using the SensiFAST probe no-ROX one step kit (Bioline). The assay for detection of PVM uses primers and probe targeting the SH gene (Forward primer ...
-
bioRxiv - Genetics 2019Quote: ... Ear punches taken to identify animals during routine colony maintenance were placed directly into Buffer A from the MyTaq Extract-PCR kit (Bioline).
-
bioRxiv - Biochemistry 2022Quote: ... First-strand cDNA synthesis and subsequent real-time PCR was performed using SensiFast ™ SYBR No-Rox One-Step Kit (Cat.No. BIO- 72005 (Bioline)) ...
-
bioRxiv - Molecular Biology 2022Quote: MtDNA copy number was quantified by quantitative PCR (qPCR) using the SensiFAST™ SYBR No-ROX Kit (BIO-98020, Bioline), by amplification of three mtDNA fragments (12S-rRNA ...
-
bioRxiv - Cell Biology 2023Quote: ... and used as a template for quantitative polymerase chain reaction with reverse transcription (qRT–PCR) analysis using SensiFAST SYBR Lo-ROX Kit (Bioline). Samples were analyzed using a QuantStudio 3 Real-Time PCR System (ThermoFisher) ...
-
bioRxiv - Microbiology 2023Quote: ... Quantitative reverse-transcription PCR (qRT-PCR) with was performed with three technical replicates using 50 ng cDNA and the SensiFAST SYBR & Fluorescein Kit (Bioline) on a Roche Lightcycler 96 instrument ...
-
bioRxiv - Cell Biology 2022Quote: ... The qRT-PCR reaction was performed using 35 µL SensiFAST™ Probe Lo- ROX One-Step Kit (Bioline, Taunton, MA) and 15 µL of extracted eluate ...
-
bioRxiv - Plant Biology 2023Quote: ... and the 200 to 400 bp range was purified by gel extraction using Isolate II PCR and Gel Kit (Bioline). Libraries were sequenced (100 bp paired-end ...
-
bioRxiv - Microbiology 2021Quote: ... and quantitative real-time-PCR (qRT-PCR) reactions were performed in triplicate (Bioline) and monitored by a Roche Lightcycler 96 ...
-
bioRxiv - Neuroscience 2020Quote: ... qRT-PCR was performed using the 2× SYBR Green PCR Master Mix (Bioline) with the CFX96 detection system and analyzed with CFX Manager softward (Bio-rad) ...
-
bioRxiv - Developmental Biology 2022Quote: ... The PCR was performed using MyTaq polymerase (Bioline; PCR program in Supplementary Table 3). If embryos showed the desired deletions ...
-
bioRxiv - Developmental Biology 2022Quote: ... The PCR was performed using MyTaq polymerase (Bioline; PCR program in Supplementary Table 3). If embryos showed deletions ...
-
bioRxiv - Cancer Biology 2019Quote: ... cDNA was amplified on quantitative PCR in a total volume of 5 μl with SensiFAST SYBR® No-ROX Kit (Bioline) and specific primers on a LightCycler 480 (Roche) ...
-
bioRxiv - Developmental Biology 2020Quote: cDNA was diluted 1:10 in nuclease-free water and subsequently applied for qRT-PCR using the SensiFast™SYBR HiROX Kit (Bioline) according to the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2021Quote: ... the K13 locus was amplified directly from whole blood using the primer pair p26+p27 (Table S9) and the MyTaq™ Blood-PCR Kit (Bioline). Primer pairs p39+p40 and p50+p51 were used to amplify fd and mdr2 ...
-
bioRxiv - Pathology 2020Quote: Wheat curl mite DNA was extracted from single mites using the MyTaq™ Extract-PCR Kit (Bioline Meridian Bioscience, London, UK)) according to the manufacturer’s recommendations with the exception of the amount of starting material being less than 3 mg ...
-
bioRxiv - Cancer Biology 2020Quote: ... Primers were designed using NCBI primer blast (Table 3) and qRT-PCR was performed using SensiFAST™ SYBR Lo-ROX Kit (#BIO-94020, Bioline) on the BIO-RAD CFX38 Real time PCR machine ...
-
bioRxiv - Physiology 2021Quote: ... the K13 locus was amplified directly from whole blood using the primer pair p26+p27 (Supplementary file 5) and the MyTaq™ Blood-PCR Kit (Bioline). Primer pairs p39+p40 and p50+p51 were used to amplify fd and mdr2 ...