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Citations for Bioline :
601 - 650 of 720 citations for QuantiChrom Aldehyde Dehydrogenase Inhibitor Screening Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: qRT-PCR was used to quantify transcript levels of specific quorum sensing-controlled genes in DS40M4 and was performed using the SensiFast SYBR Hi-ROX One-Step Kit (Bioline) according to the manufacturer’s guidelines ...
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bioRxiv - Microbiology 2021Quote: Real-time qRT-PCR (quantitative reverse transcription PCR) was used to quantify transcript levels using the SensiFast SYBR Hi-ROX One-Step Kit (Bioline) according to the manufacturer’s guidelines ...
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bioRxiv - Cancer Biology 2021Quote: ... and qPCR reactions were conducted using gene-specific primers with SensiFAST SYBR No-ROX kit (Bioline, #Cat no: BIO-98005) as described previously (Sibai et al. ...
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bioRxiv - Immunology 2022Quote: ... Complementary DNA was synthesized using 2 µg of RNA and the Tetro cDNA synthesis kit (Bioline Reagents Ltd., London, UK) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... Extracted RNA was subsequently assayed for a short fragment of the influenza A matrix gene (Spackman et al. 2002) using the SensiFAST Probe Lo-Rox qPCR Kit (Bioline). A cycle threshold (Ct ...
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bioRxiv - Developmental Biology 2021Quote: ... washed in water and 100% ethanol prior to RNA extraction using the ISOLATE II RNA Mini Kit’s protocol (Bioline, UK). The extracted RNA was quantified using Nanodrop (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2020Quote: ... Detection and quantification of viruses was conducted by qRT-PCR using the SensiFAST probe no-ROX one step kit (Bioline). The assay for detection of PVM uses primers and probe targeting the SH gene (Forward primer ...
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bioRxiv - Molecular Biology 2020Quote: ... qPCRs were run using 2 µl cDNA (diluted 1:1) in a 10 µl reaction with 5 µl SensiFAST SBYR No-ROX Kit (Bioline) and 0.1 µM of each primer ...
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bioRxiv - Genetics 2019Quote: ... Ear punches taken to identify animals during routine colony maintenance were placed directly into Buffer A from the MyTaq Extract-PCR kit (Bioline).
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bioRxiv - Molecular Biology 2020Quote: ... The purified PCR product was run on a 1% agarose gel cut out to remove remaining primer dimers and cleaned with PCR Isolate II PCR and Gel Kit (Bioline). The purified libraries were sequenced on an Illumina HiSeq2500 or MiSeq depending on the expected complexity of the library.
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bioRxiv - Immunology 2021Quote: ... Complementary DNA was synthesized using 2 μg of RNA and the Tetro cDNA synthesis kit (Bioline Reagents Ltd., London, UK) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... Samples were ground to a fine powder in liquid nitrogen and total RNA was isolated with the isolate II RNA Mini Kit (Bioline). After DNase treatment (Promega) ...
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bioRxiv - Neuroscience 2021Quote: ... The cDNA was then diluted and used as template for qPCR, which was performed with a StepOnePlus instrument (Applied Biosystem, USA) and SensiFAST SYBR Hi-ROX Kit (Bioline) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... The real-time quantitative PCR (RT-qPCR) analysis was carried out by using the 2x SensiMix PLUS SYBR No-ROX Kit (Bioline) in a Rotor-Gene 6000 (Corbett ...
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bioRxiv - Cell Biology 2020Quote: Real-Time PCR analysis was performed using TaqMan gene expression assays using primers (see Resources Table) and SensiFast Probe Lo-ROX Kit (cat.# BIO-84020, Bioline) following manufacturer’s recommendation ...
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bioRxiv - Cell Biology 2021Quote: mRNA was isolated using NucleoSpin® RNA Plus (Machery-Nagel) and 1 µg of total mRNA was reverse-transcribed into cDNA using SensiFASTTM cDNA Synthesis Kit (Bioline) according to the manufacture’s protocol ...
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bioRxiv - Biochemistry 2022Quote: ... First-strand cDNA synthesis and subsequent real-time PCR was performed using SensiFast ™ SYBR No-Rox One-Step Kit (Cat.No. BIO- 72005 (Bioline)) ...
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bioRxiv - Genetics 2022Quote: ... 1 μg of RNA was then reverse transcribed with random hexamer and anchored oligo dT primers using SensiFast cDNA synthesis kit (Bioline). Quantitative PCR was performed on the diluted cDNA using SensiFast probe (Bioline ...
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bioRxiv - Genetics 2022Quote: The frequency of proximity ligated fragments containing the Myc promoter and other downstream regions was then quantified on the BioRad C1000 Thermo cycler using the SensiMix SYBR NOROX kit (Bioline) with 60°C annealing temperature ...
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bioRxiv - Molecular Biology 2022Quote: MtDNA copy number was quantified by quantitative PCR (qPCR) using the SensiFAST™ SYBR No-ROX Kit (BIO-98020, Bioline), by amplification of three mtDNA fragments (12S-rRNA ...
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bioRxiv - Molecular Biology 2022Quote: ... ChIP DNA and input DNA were diluted 5X and amplified using 500 nM primers and SensiFAST SYBR (No-ROX Kit, Bioline) using a LightCycler 480 (Roche).
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bioRxiv - Microbiology 2022Quote: ... 500-ng portions of DNA extracted from each larva were subjected to qPCR using the SensiMic SybR Green kit (Bioline). Forward (5′-ACTTCCGCAATGGACGTTAC-3′ ...
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bioRxiv - Microbiology 2023Quote: ... Reverse transcription and quantitative real-time PCRs (qRT-PCR) were performed using the SensiFast SYBR No-ROX One Step kit (Bioline) in a Rotor-Gene Q lightcycler (Qiagen) ...
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bioRxiv - Cell Biology 2023Quote: ... and used as a template for quantitative polymerase chain reaction with reverse transcription (qRT–PCR) analysis using SensiFAST SYBR Lo-ROX Kit (Bioline). Samples were analyzed using a QuantStudio 3 Real-Time PCR System (ThermoFisher) ...
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bioRxiv - Cell Biology 2023Quote: ... RNA was isolated from lung homogenates from mice exposed to E-cigarettes (with and without nicotine) and room air (SHAM) using Isolate II RNA Mini Kit (Bioline) and cDNA was synthesized using SensiFAST cDNA Synthesis kit (Bioline) ...
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bioRxiv - Genomics 2023Quote: ... 1 μg of the entry vector was also digested with EcoRI-HF and NheI-HF and the linearised product was purified from a 2% agarose gel using PCR Isolate II PCR and Gel Kit (Bioline). The digested and purified reporter pool was then ligated into 80 ng of the linearised entry vector using Takara ligation kit v1.0 (#6021 ...
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bioRxiv - Cancer Biology 2023Quote: ... 1 μl of the dilution was mixed with 0.41 μM primers (Supplemental Table S4) and 1x Sensifast SYBR non-Rox kit (Bioline Bio98005). The real-time PCR program cycled as follows ...
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bioRxiv - Microbiology 2023Quote: ... Quantitative reverse-transcription PCR (qRT-PCR) with was performed with three technical replicates using 50 ng cDNA and the SensiFAST SYBR & Fluorescein Kit (Bioline) on a Roche Lightcycler 96 instrument ...
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bioRxiv - Cell Biology 2022Quote: ... The qRT-PCR reaction was performed using 35 µL SensiFAST™ Probe Lo- ROX One-Step Kit (Bioline, Taunton, MA) and 15 µL of extracted eluate ...
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bioRxiv - Cancer Biology 2023Quote: ... Isolated RNA (0.5-1 µg) was then used for reverse transcription into first strand cDNA via a SensiFast cDNA synthesis kit (Bioline). RNA spike I (TATAA ...
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bioRxiv - Evolutionary Biology 2022Quote: qPCR was performed on each of the samples using an Applied Biosystems StepOnePlus system with a Sensifast Hi-Rox Sybr kit (Bioline). Cycle conditions were as follows ...
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bioRxiv - Evolutionary Biology 2022Quote: ... qRT-PCR was carried out on 1:2 diluted cDNA on an Applied Biosystems StepOnePlus system using a Sensifast Hi-Rox SYBR kit (Bioline). Cycle conditions were as follows ...
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bioRxiv - Evolutionary Biology 2023Quote: The relative quantity of the reverse transcribed target cDNA was assessed using magnetic induction cycler (micPCR, Bio Molecular Systems) and SensiFAST SYBR Lo-ROX Kit (Bioline). For each gene ...
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bioRxiv - Plant Biology 2023Quote: ... and the 200 to 400 bp range was purified by gel extraction using Isolate II PCR and Gel Kit (Bioline). Libraries were sequenced (100 bp paired-end ...
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bioRxiv - Microbiology 2023Quote: ... 0.2 ml chloroform was added for phase separation using phase lock gel heavy tubes and RNA isolation from the aqueous phase was obtained by using ISOLATE II RNA Mini Kit (Bioline). RNA quality was verified with Agilent 2100 Bioanalyzer System using RNA Nano Chips (Agilent Technologies) ...
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bioRxiv - Microbiology 2023Quote: ... If a band was visualised at ∼480 bp the PCR product was purified using an ISOLATE II PCR and Gel Kit (Bioline).
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bioRxiv - Microbiology 2023Quote: ... PCR products that produced bands of the correct size were purified using the ISOLATE II PCR and Gel Kit (Bioline) as per manufacturer’s protocol and submitted for sequencing using an Applied Biosystems 3730xl capillary analyser (Macrogen) ...
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bioRxiv - Microbiology 2023Quote: ... was performed in technical triplicate with 50 ng of cDNA per reaction using the SensiFAST SYBR & Fluorescein Kit from Bioline, on a Roche Lightcycler 96 ...
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bioRxiv - Neuroscience 2023Quote: ... or 1 μg (4 weeks of treatment) of the total RNA was reverse-transcribed into cDNA using a Tetro cDNA synthesis kit (BIO-65042, Bioline). Quantitative PCR (RT-qPCR ...
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bioRxiv - Microbiology 2023Quote: ... 0.2 ml chloroform was added for phase separation using phase lock gel heavy tubes and RNA isolation from the aqueous phase was obtained by using ISOLATE II RNA Mini Kit (Bioline). RNA quality was verified with Agilent 2100 Bioanalyzer System using RNA Nano Chips (Agilent Technologies) ...
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bioRxiv - Microbiology 2023Quote: ... The reactions (10 μL total volume) contained 5 μL of 2 × SensiFAST™ SYBR No-ROX Kit (Bioline, London, UK), 0.2 μM of each arsM gene primer ...
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bioRxiv - Cell Biology 2023Quote: ... 150 ng of total RNA was converted to complementary DNA (cDNA) using SensiFASTTM cDNA synthesis kit following manufacturer instructions (Bioline) and qPCR performed using SensiFAST™ SYBR® Lo-ROX Kit following manufacturer instructions (Bioline) ...
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bioRxiv - Plant Biology 2024Quote: ... Quantitative Real-time PCR (qRT-PCR) was carried out using SensiFAST™ SYBR® No-ROX One-Step Kit (Bioline) and the results were analysed using the 2−ΔΔCt method (Livak & Schmittgen ...
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bioRxiv - Neuroscience 2024Quote: ... 150 ng of total RNA was converted to complementary DNA (cDNA) using SensiFASTTM cDNA synthesis kit following manufacturer instructions (Bioline) and qPCR performed in technical triplicate using SensiFAST™ SYBR® Lo-ROX Kit following manufacturer instructions (Bioline ...
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bioRxiv - Cell Biology 2020Quote: ... PCR bands were extracted from the agarose gel and purified using and Isolate II PCR and gel kit (Bioline, London, UK) and sent for Sanger sequencing (GATC Biotech ...
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bioRxiv - Microbiology 2021Quote: ... Each reaction (20 μL) consisted of 1 μL of cDNA substrate and 19 μL of a SensiFAST No-ROX Kit Master Mix (Bioline, UK). Following primer optimisation ...
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bioRxiv - Genetics 2019Quote: ... cDNA was diluted in nuclease-free water and stored at −20°C until used in qPCR with the SensiFastTM SYBR® No-ROX kit (Bioline) and a Lightcycler® 480/1536 (Roche ...
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bioRxiv - Cancer Biology 2019Quote: ... cDNA was amplified on quantitative PCR in a total volume of 5 μl with SensiFAST SYBR® No-ROX Kit (Bioline) and specific primers on a LightCycler 480 (Roche) ...
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bioRxiv - Developmental Biology 2020Quote: cDNA was diluted 1:10 in nuclease-free water and subsequently applied for qRT-PCR using the SensiFast™SYBR HiROX Kit (Bioline) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2019Quote: ... The amount of M and NA segment was determined with one-step RT-qPCR using SensiFAST™ Probe Lo-ROX One-Step Kit (Bioline). The sequences of primers and probe for M segment detection are AGA TGA GYC TTC TAA CCG AGG TCG (forward primer) ...