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Citations for Bioline :
1 - 50 of 416 citations for PCR Tubes since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: For each reaction 1μL of gDNA solution was suspended in a PCR tube with 2μL of MyTaq red reaction buffer (5x; Bioline), 1µL of pooled primers/oligonucleotides (final concentration of each primer 10μM or 5μM for Ubc-Cre) ...
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bioRxiv - Synthetic Biology 2021Quote: ... We performed colony PCR using BioRed PCR mix (Bioline) to screen for genome modifications ...
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bioRxiv - Genomics 2023Quote: ... PCR products purified with Isolate II PCR and Gel Kit (BIOLINE) and cloned into the entry vector pCR®8/GW/TOPO (#250020 Invitrogen ...
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bioRxiv - Microbiology 2021Quote: ... After PCR verification in agarose gel and PCR clean-up using the ISOLATE II PCR and Gel Kit (Bioline), the remaining plasmid template was digested with DpnI at 37°C for 3h ...
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bioRxiv - Molecular Biology 2020Quote: ... and purified using the PCR Isolate II PCR and Gel Kit (Bioline) or by ExoSAP (for primers see Table S4) ...
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bioRxiv - Immunology 2020Quote: ... PCRs were performed with the IMMOLASE™ DNA polymerase PCR kit (Bioline), and the success of amplification was assessed by agarose gel electrophoresis ...
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bioRxiv - Microbiology 2021Quote: ... and quantitative real-time-PCR (qRT-PCR) reactions were performed in triplicate (Bioline) and monitored by a Roche Lightcycler 96 ...
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bioRxiv - Neuroscience 2020Quote: ... qRT-PCR was performed using the 2× SYBR Green PCR Master Mix (Bioline) with the CFX96 detection system and analyzed with CFX Manager softward (Bio-rad) ...
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bioRxiv - Molecular Biology 2021Quote: ... Resulting PCR products were purified on columns (Isolate II PCR Kit (BIO-52059, Bioline)) that are suitable for >50 bp DNA fragments ...
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bioRxiv - Microbiology 2021Quote: ... PCR products were purified by using the Isolate II PCR and gel kit (Bioline), analyzed by agarose gel electrophoresis and then sequenced (GATC Biotech ...
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bioRxiv - Developmental Biology 2022Quote: ... The PCR was performed using MyTaq polymerase (Bioline; PCR program in Supplementary Table 3). If embryos showed the desired deletions ...
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bioRxiv - Developmental Biology 2022Quote: ... The PCR was performed using MyTaq polymerase (Bioline; PCR program in Supplementary Table 3). If embryos showed deletions ...
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bioRxiv - Molecular Biology 2024Quote: ... real-time PCR was conducted using the SensiFAST SYBR real-time PCR kit (Bioline). The obtained data were normalized to the human endogenous control (GAPDH ...
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bioRxiv - Molecular Biology 2022Quote: ... The PCR amplicon band was cut from the gel and isolated by PCR Isolate II PCR and Gel Kit (Bioline, cat. no. BIO-52060) and lastly bead-purified ...
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bioRxiv - Microbiology 2021Quote: ... The PCR mix was composed of 5 μL of 5X MyTaq red PCR buffer (Bioline), 1.25 μL of 25 mM MgCl2 ...
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bioRxiv - Zoology 2019Quote: ... PCR products were purified with an ISOLATE II PCR and Gel Kit (Bioline, BIO-52059) according to the manufacturers guidelines ...
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bioRxiv - Genomics 2023Quote: ... PCR products were purified using the ISOLATE II PCR and Gel Kit (Bioline, BIO-52060) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... PCR products were purified using the ISOLATE II PCR and Gel Kit (Bioline, BIO-52060) according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2021Quote: ... PCR products were extracted from the agarose gel with ISOLATE II PCR and Gel Kit (Bioline) and Sanger sequenced.
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bioRxiv - Microbiology 2021Quote: ... the 15 μl PCR reaction mixture contained 1x MyTaq™ Red PCR Mix (Bioline, South Africa), 0.2 μM primers ...
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bioRxiv - Molecular Biology 2020Quote: ... and PCR amplification (Bioline, Taunton, MA). The libraries were sequenced by multiplexing 8 libraries per lane on the Illumina HiSeq2500 sequencer ...
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bioRxiv - Microbiology 2020Quote: ... MyTaq Red PCR Mix (Bioline, UK) and 200 nM sense/anti-sense primers and amplification following 35 cycles of 95°C ...
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bioRxiv - Plant Biology 2024Quote: ... PCR was performed using MyTaq (Bioline) DNA Polymerase in reactions containing 30ng of DNA template ...
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bioRxiv - Bioengineering 2023Quote: ... My Taq PCR master mix (Bioline) that included buffer ...
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bioRxiv - Plant Biology 2019Quote: ... The 15 μl PCR cocktail consisted of 7.5 μl of PCR MyTaq Red Mix (Bioline, GmbH, Germany), 1.0 μl of genomic DNA template (70 ng/μl) ...
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bioRxiv - Microbiology 2020Quote: PCR products were purified using a commercial kit (Isolate II PCR and Gel Kit, Bioline, London, UK) and sent for sequencing (Macrogen Europe ...
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bioRxiv - Microbiology 2020Quote: ... Quantitative PCR or RT-PCR analyses were performed using Brilliant III SYBR green QPCR master mix (Bioline) with gene-specific primers on a Applied Bioscience StepOnePlus qPCR machine (Life Technologies) ...
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bioRxiv - Microbiology 2021Quote: ... The 15 µl PCR reaction mixture contained 1x of MyTaq ™ Red PCR Mix (Bioline, South Africa), primers at 0.2 µM ...
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bioRxiv - Microbiology 2020Quote: ... PCR products were purified using the Bioline Isolate II PCR and Gel kit (Bioline, Cat. No. BIO-52060). Samples were sequenced using the Illumina Miseq platform ...
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bioRxiv - Pathology 2019Quote: ... PCR amplicons of each sample were pulled together and purified using Isolate II PCR and gel Kit (BIOLINE) and about 100 ng of DNA was used in library preparation ...
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bioRxiv - Neuroscience 2021Quote: ... In short myTaq extract-PCR kit (Bioline) was used to amplify a 300 bp fraction of the ath5 gene containing the lakritz mutation using the following primers ...
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bioRxiv - Physiology 2020Quote: ... using SYBR Green PCR master mix (Bioline). Data is represented as 2^-ΔCt compared to average of PC1/3 in healthy group ...
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bioRxiv - Synthetic Biology 2020Quote: ... PCR reactions were conducted using MyTaq (Bioline) DNA polymerase ...
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bioRxiv - Genetics 2020Quote: ... or MyTaq™ Extract-PCR Kit (Bioline). gDNA was prepared from blastocysts in a 20 µL solution of 185.5 mM pH 8.3 Tris-HCl ...
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Psi promotes Drosophila wing growth through transcriptional repression of key developmental networksbioRxiv - Developmental Biology 2020Quote: ... PCR using MyTaq polymerase (Bioline BIO-21113) was performed with 3 long extension cycles followed by 17 short extension cycles as described (Vogel ...
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bioRxiv - Genetics 2020Quote: ... Genotyping was performed by PCR of genomic tail DNA using the Extract PCR kit (Bioline, cat. No. BIO-21127). All mice were kept on C57BL/6J (The Jackson Laboratory ...
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bioRxiv - Plant Biology 2022Quote: ... PCR products were purified from agarose gels using the ISOLATE II PCR and Gel Kit (Bioline, Memphis, TN, USA), and quantified in a NanoDrop One C Spectrophotometer (Thermo Scientific ...
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bioRxiv - Neuroscience 2021Quote: Genomic DNA extraction from tail snips and genotyping PCR reactions were performed using MyTaq Extract-PCR kit (Bioline, Taunton, MA). All the mice were genotyped by the fragment PCR method as indicated in the Jackson Laboratory website ...
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bioRxiv - Molecular Biology 2020Quote: ... cDNA products were used as the template for quantitative reverse transcription-PCR (qRT-PCR) using the SensiMix SYBR No-ROX kit (Bioline). For relative quantification of gene expression ...
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bioRxiv - Microbiology 2019Quote: ... Reverse transcription and quantitative real-time PCRs (qRT-PCR) were performed using the SensiFast SYBR No-ROX One Step kit (Bioline) in a Rotor-Gene Q Lightcycler (Qiagen) ...
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bioRxiv - Microbiology 2020Quote: ... Influenza virus M gene copy number per μL RNA was determined by qRT-PCR using the SensiFAST Probe Lo-ROX One-Step qRT-PCR System Kit (Bioline) on the ABI 7500 Real Time PCR System (Applied Biosystems ...
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bioRxiv - Microbiology 2021Quote: ... Quantitative real time-PCR (qRT-PCR) analysis was performed in triplicate on 50 ng cDNA using the SensiFAST SYBR & Fluorescein kit (Bioline) and a Roche Lightcycler 96 ...
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bioRxiv - Physiology 2020Quote: ... Genomic DNA extraction from tails snips and genotyping PCR reactions were performed using MyTaq Extract-PCR kit (Bioline, Taunton, MA). Mice were genotyped using a fragment PCR method with the primers shown in the table below and indicated on the Jackson Laboratory website ...
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bioRxiv - Microbiology 2020Quote: ... PCR products were generated in 25 µl reactions containing 12.5 µl Bioline PCR Master Mix (Bioline USA Inc, Taunton, MA), 10.0 µl MG H2O ...
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bioRxiv - Molecular Biology 2020Quote: ... The purified PCR product was run on a 1% agarose gel cut out to remove remaining primer dimers and cleaned with PCR Isolate II PCR and Gel Kit (Bioline). The purified libraries were sequenced on an Illumina HiSeq2500 or MiSeq depending on the expected complexity of the library.
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bioRxiv - Plant Biology 2024Quote: ... Quantitative Real-time PCR (qRT-PCR) was carried out using SensiFAST™ SYBR® No-ROX One-Step Kit (Bioline) and the results were analysed using the 2−ΔΔCt method (Livak & Schmittgen ...
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bioRxiv - Microbiology 2023Quote: ... Reverse transcription and quantitative real-time PCRs (qRT-PCR) were performed using the SensiFast SYBR No-ROX One Step kit (Bioline) in a Rotor-Gene Q lightcycler (Qiagen) ...
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bioRxiv - Genomics 2023Quote: ... 1 μg of the entry vector was also digested with EcoRI-HF and NheI-HF and the linearised product was purified from a 2% agarose gel using PCR Isolate II PCR and Gel Kit (Bioline). The digested and purified reporter pool was then ligated into 80 ng of the linearised entry vector using Takara ligation kit v1.0 (#6021 ...
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bioRxiv - Microbiology 2023Quote: ... If a band was visualised at ∼480 bp the PCR product was purified using an ISOLATE II PCR and Gel Kit (Bioline).
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bioRxiv - Microbiology 2023Quote: ... PCR products that produced bands of the correct size were purified using the ISOLATE II PCR and Gel Kit (Bioline) as per manufacturer’s protocol and submitted for sequencing using an Applied Biosystems 3730xl capillary analyser (Macrogen) ...