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Citations for Bioline :
101 - 150 of 194 citations for NCAM 1 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2023Quote: ... 1’ 72°C) by MyTaq Red mix (Bioline) using primers that add overhangs with EcoRI (MT024 ...
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bioRxiv - Genetics 2023Quote: ... Hyperladder 50 bp or Hyperladder 1 kb (Bioline) served as DNA fragment size markers.
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bioRxiv - Immunology 2023Quote: ... The total qPCR reaction volume was 1.5 μl and consisted of 0.5 μl of cDNA (dilution 1/12) and 1 μl of SensiFAST™ SYBR® No-ROX Kit (Bioline) containing 0.4 μM of PCR primer (Eurogenetec SA)(primer list in sup ...
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bioRxiv - Microbiology 2019Quote: ... A second round of amplification was performed by transferring 5μl of 1:100 diluted Round 1 product into 15μl Bioline MyFi™ Mix (BIOLINE GmbH, Luckenwalde, Germany) reactions.
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bioRxiv - Immunology 2023Quote: ... the aqueous phase was mixed 1:1 v/v with 70% ethanol and loaded into a column of the Isolate II RNA Mini kit (Bioline, Cincinnati, OH). Total RNA was purified following the manufacturer’s recommendations ...
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bioRxiv - Molecular Biology 2021Quote: ... The final volume contained 1× SensiMix (Bioline, London, UK), 800 nM of each primer and 200nM of each probe and the PCR cycling conditions included an initial denaturation at 95°C for 10 minutes ...
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bioRxiv - Genomics 2023Quote: ... Samples were compared to 1 Kb hype ladder (Bioline). The primer sequences are listed in Table S9.
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bioRxiv - Cell Biology 2023Quote: ... and 1 unit of Taq polymerase (Bioline, Meridian Bioscience). The PCR conditions were an initial melting step of 94°C for 4 min ...
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bioRxiv - Microbiology 2019Quote: ... The 1 kb molecular weight marker (HyperLadder IV, Bioline, UK) was used in together with the amplified products ...
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bioRxiv - Microbiology 2020Quote: ... for 1 minute in 0.2 mL TRIsure (Bioline: BIO-38033). Homogenized tissue was brought to 1 mL volume with 0.8 mL TRIsure ...
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bioRxiv - Genetics 2020Quote: ... and 1 Unit MyTaqTM (Bioline; Meridian Bioscience Inc., London, UK) by incubation for 2 min at 96°C ...
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bioRxiv - Genomics 2023Quote: ... supplemented with 1:100 10 mg/mL Proteinase K (Bioline). TIDE was performed as described before (40) ...
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bioRxiv - Microbiology 2021Quote: ... using 5 μl product with 1 μl 5X loading dye (BioLine) to verify bands of approximately 450 bp ...
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bioRxiv - Synthetic Biology 2023Quote: ... Molecular weight markers used were the 1 kb HyperLadder (from Bioline). T4 DNA ligase and buffer were purchased from New England Biolabs ...
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bioRxiv - Molecular Biology 2023Quote: ... The resultant bands were compared to a 1 Kb HyperLadder (Bioline) using the expected sizes per gene region as follows ...
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bioRxiv - Molecular Biology 2020Quote: ... buffer with 1 mg/ml Proteinase K (Bioline, Cat. No. BIO-37084) to pre-lyse the cells ...
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bioRxiv - Microbiology 2019Quote: ... A 1 kilobase molecular-weight size marker (Hyperladder 1KB from Bioline UK) was used to confirm that the 16S ribosomal RNA hypervariable region had been isolated (supplementary material) ...
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bioRxiv - Microbiology 2021Quote: ... 1% SDS (Sigma-Aldrich)] supplemented with 5 μL Proteinase K (20 mg mL−1; Bioline Australia) to lyse cells ...
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bioRxiv - Molecular Biology 2022Quote: ... supplemented with 1 mg/ml proteinase K (Bioline, cat. no. BIO-37084) by incubating them at 55 °C for at least 2 hours up to overnight ...
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bioRxiv - Molecular Biology 2020Quote: ... or HyperLadder 1 kb (range 200 bp to 10 kb, BIO-33053, Bioline) to determine product sizes.
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bioRxiv - Plant Biology 2020Quote: ... The PCR product was visualized on a 1% (w/v) agarose gel (Bioline) by electrophoresis at 90 V for 1 h and the 1.5 kb target band was collected for purification using NucleoSpin® Gel and PCR Clean-up kit (Macherey-Nagel ...
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bioRxiv - Synthetic Biology 2022Quote: ... each well contained 1 mL LB agar supplemented with 2 mM IPTG (Bioline), 5 μg/mL chloramphenicol (MilliporeSigma) ...
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bioRxiv - Biochemistry 2022Quote: ... RNA samples (1 µg) were reverse transcribed using SensiFAST cDNA synthesis kit (Bioline) according to manufacturer’s protocol and subjected to Real-time quantitative PCR (RT-qPCR ...
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bioRxiv - Plant Biology 2020Quote: ... 1 μg of total RNA was reverse transcribed with SensiFAST cDNA Synthesis Kit (Bioline) according to the manufacturer’s recommendations ...
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bioRxiv - Plant Biology 2020Quote: ... 2 μl of 1:10 diluted cDNA and BIOTAQ™ DNA polymerase (Bioline GmbH) were used in a CFX96™ Real-Time PCR Detection System (Bio-Rad Laboratories ...
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bioRxiv - Plant Biology 2020Quote: ... The final PCR products were visualized on a 1% (w/v) agarose gel (Bioline) by electrophoresis at 90 V for 1 h ...
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bioRxiv - Biochemistry 2022Quote: ... cDNA was synthesized from 1 µg RNA samples using SensiFAST cDNA synthesis kit (Bioline) according to manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2022Quote: Whole frozen iBAT samples were homogenised in 1 ml TRIsure (Bioline, Memphis, Tennessee, USA) per animal using a tabletop homogeniser (FastPrep-24 5G ...
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bioRxiv - Genomics 2023Quote: ... supplied with 1:100 10 mg/mL Proteinase K (Bioline, cat. no. BIO-37084). The cells were lysed by incubating for 3h at 55°C and the Proteinase K was inactivated for 10 min at 95°C ...
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bioRxiv - Genomics 2023Quote: ... for 1 hour at 37°C and proteinase K (40 ug, Bioline BIO-37084) for 2 hours at 55°C ...
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bioRxiv - Developmental Biology 2021Quote: ... cDNA was synthesized from 1 µg total RNA using cDNA synthesis kit (Bioline, Sydney, Australia) and RT-PCR was performed using the SYBR® Select Master Mix (Thermofisher Scientific ...
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bioRxiv - Immunology 2020Quote: ... Reverse transcription of 1 μg of total RNA was performed using Tetro reverse transcriptase (Bioline). For reverse transcription ...
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bioRxiv - Cancer Biology 2022Quote: ... fresh frozen tumor tissue were placed in 1 ml of TRIsure reagent (#BIO-38032, Bioline) and tissue lysis was achieved by high-speed shaking with stainless steel beads for 10 min ...
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bioRxiv - Molecular Biology 2021Quote: ... cDNA was synthesized from 1 μg of purified RNA with a SensiFast cDNA synthesis kit (Bioline). The primers used are listed in Table S2 (Supplementary Material) ...
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bioRxiv - Microbiology 2019Quote: ... The size and quality of extracted DNA was checked by electrophoresis in 1% agarose gel (Bioline) including GelRed™ (Biotium) ...
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bioRxiv - Neuroscience 2020Quote: ... 0.5-1 μg of total RNA was retrotranscribed using SensiFAST cDNA Synthesis Kit (BioLine, London, UK). Real-time PCR was performed using iTaq qPCR master mix according to manufacturer’s instructions (Bio-Rad ...
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bioRxiv - Cancer Biology 2021Quote: ... For qPCR reaction 1 μg of cDNA was used with SensiFast Sybr Lo-Rox mix (Bioline) and respective primer pair ...
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bioRxiv - Cancer Biology 2023Quote: ... which were subsequently separated using 1% agarose gels prepared with molecular grade agarose (Bioline, BIO-41025), Tris base-acetic acid-EDTA (TAE ...
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bioRxiv - Cancer Biology 2022Quote: ... Isolated RNA was used (1 μg) to make cDNA using SensiFASTTM Kit (Bioline Cat. Bio-65053). qPCR master mix was prepared using iTaq universal SYBR green supermix (Bio-Rad Cat ...
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bioRxiv - Cancer Biology 2023Quote: ... 1 mg of total RNA was retrotranscribed in cDNA using the SensiFAST cDNA Synthesis KIT (Bioline). Specific sets of primer pairs were designed and tested with primerBLAST (NCBI ...
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bioRxiv - Molecular Biology 2023Quote: ... and diluted 1:10 before quantification using the SensiFAST SYBR No-Rox kit (Bioline, BIO-98005). qRT-PCR was performed with 1 μM of each primer (a common reverse primer for both Trm1 isoforms and isoform-specific forward primers ...
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bioRxiv - Cancer Biology 2024Quote: ... cDNA was generated from 1 µg of RNA using the SensiFAST cDNA synthesis kit (Bioline, #BIO65054). cDNA was then diluted (1:5 ...
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Safety and efficacy of C9ORF72-repeat RNA nuclear export inhibition in amyotrophic lateral sclerosisbioRxiv - Systems Biology 2021Quote: ... Cells were then lysed in hypotonic lysis buffer containing 0.16 U µl−1 Ribosafe RNase inhibitors (Bioline), 2 mM PMSF and SIGMAFAST Protease Inhibitor Cocktail tablets ...
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bioRxiv - Molecular Biology 2020Quote: ... including 10 μl of 10 mg/ml BSA solution and 5 μl of HyperLadder 1 kb (BIOLINE), 200 μl of the filtered cell lysates was injected onto the pre-equilibrated SEC column ...
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bioRxiv - Physiology 2021Quote: ... cDNA synthesis was performed with 1 µg RNA (SensiFAST™ cDNA Synthesis Kit, Bioline, Cat# BIO-65053), according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2020Quote: ... Table 1) were used in a pre-optimised PCR master mix (BioMix™, Bioline, Meridian Bioscience; Australia) following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... and 1 µg of RNA was reverse transcribed to cDNA using SensiFAST cDNA Synthesis Kit (Bioline #65054). qPCR reactions were set up using PowerUp SYBR Green Master Mix (Applied Biosystems #A25742 ...
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bioRxiv - Cell Biology 2020Quote: ... cDNA was retrotranscribed from 0.3 µg to 1 µg using the SensiFAST cDNA Synthesis Kit (Bioline, BIO-65054). Real-time PCR was performed on the CFX384 Touch Real-time PCR Detection System (Bio-Rad ...
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bioRxiv - Genetics 2021Quote: ... 1 µg of total RNA was reverse transcribed using SensiFAST cDNA Synthesis Kit following the manufacturer’s protocol (Bioline).
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bioRxiv - Microbiology 2021Quote: ... The PCR products were analysed by 0.8% agarose-gel electrophoresis with 1 kb ladder (Bioline, Cat#H1-819101A), run at 100 V for 60 min ...