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Citations for Bioline :
1 - 50 of 807 citations for Mouse Alpha 1 antitrypsin 1 3 SERPINA1C ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... 2 µL 1:1 diluted cDNA and SensiFAST SYBR No-ROX Kit (#BIO-98005, Bioline). Knockdown was assessed using ΔΔCt analysis as described by Pfaffl [92].
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bioRxiv - Microbiology 2019Quote: ... coli (alpha-select competent cells, Bioline or XL1-Blue, Stratagene) and plasmids were purified using Zyppy Plasmid Kits (Zymo Research Corporation) ...
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bioRxiv - Molecular Biology 2020Quote: ... qPCRs were run using 2 µl cDNA (diluted 1:1) in a 10 µl reaction with 5 µl SensiFAST SBYR No-ROX Kit (Bioline) and 0.1 µM of each primer ...
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bioRxiv - Immunology 2023Quote: ... The total qPCR reaction volume was 1.5 μl and consisted of 0.5 μl of cDNA (dilution 1/12) and 1 μl of SensiFAST™ SYBR® No-ROX Kit (Bioline) containing 0.4 μM of PCR primer (Eurogenetec SA)(primer list in sup ...
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bioRxiv - Neuroscience 2022Quote: ... Dehydrated samples were rehydrated through a series of methanol/PBS washes for 10 minutes each at room temperature with an additional 3 x 10 minutes PBSTw (0.1% Tween-20) and treated with proteinase K (1:1000, Bioline) for 20 minutes to increase permeabilization ...
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bioRxiv - Immunology 2023Quote: ... the aqueous phase was mixed 1:1 v/v with 70% ethanol and loaded into a column of the Isolate II RNA Mini kit (Bioline, Cincinnati, OH). Total RNA was purified following the manufacturer’s recommendations ...
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bioRxiv - Biochemistry 2022Quote: ... RNA samples (1 µg) were reverse transcribed using SensiFAST cDNA synthesis kit (Bioline) according to manufacturer’s protocol and subjected to Real-time quantitative PCR (RT-qPCR ...
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bioRxiv - Plant Biology 2020Quote: ... 1 μg of total RNA was reverse transcribed with SensiFAST cDNA Synthesis Kit (Bioline) according to the manufacturer’s recommendations ...
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bioRxiv - Biochemistry 2022Quote: ... cDNA was synthesized from 1 µg RNA samples using SensiFAST cDNA synthesis kit (Bioline) according to manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2021Quote: ... cDNA was synthesized from 1 µg total RNA using cDNA synthesis kit (Bioline, Sydney, Australia) and RT-PCR was performed using the SYBR® Select Master Mix (Thermofisher Scientific ...
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bioRxiv - Molecular Biology 2021Quote: ... cDNA was synthesized from 1 μg of purified RNA with a SensiFast cDNA synthesis kit (Bioline). The primers used are listed in Table S2 (Supplementary Material) ...
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bioRxiv - Neuroscience 2020Quote: ... 0.5-1 μg of total RNA was retrotranscribed using SensiFAST cDNA Synthesis Kit (BioLine, London, UK). Real-time PCR was performed using iTaq qPCR master mix according to manufacturer’s instructions (Bio-Rad ...
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bioRxiv - Cancer Biology 2022Quote: ... Isolated RNA was used (1 μg) to make cDNA using SensiFASTTM Kit (Bioline Cat. Bio-65053). qPCR master mix was prepared using iTaq universal SYBR green supermix (Bio-Rad Cat ...
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bioRxiv - Cancer Biology 2023Quote: ... 1 mg of total RNA was retrotranscribed in cDNA using the SensiFAST cDNA Synthesis KIT (Bioline). Specific sets of primer pairs were designed and tested with primerBLAST (NCBI ...
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bioRxiv - Molecular Biology 2023Quote: ... and diluted 1:10 before quantification using the SensiFAST SYBR No-Rox kit (Bioline, BIO-98005). qRT-PCR was performed with 1 μM of each primer (a common reverse primer for both Trm1 isoforms and isoform-specific forward primers ...
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bioRxiv - Cancer Biology 2024Quote: ... cDNA was generated from 1 µg of RNA using the SensiFAST cDNA synthesis kit (Bioline, #BIO65054). cDNA was then diluted (1:5 ...
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bioRxiv - Evolutionary Biology 2023Quote: ... from Mediterranean and temperate species had germinated we transplanted up to 20 seedlings each into 9 × 9 cm pots (one plant per pot) with a 3:1 mixture of potting soil (Einheitserde®, BioLine, Topfsubstrat Öko torffrei) and sand (0-2 mm play sand ...
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bioRxiv - Genomics 2020Quote: ... 2 □1 water and 5□1 MyTaq™ HS Mix (Bioline). RT-PCR was performed in the following conditions ...
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bioRxiv - Physiology 2021Quote: ... cDNA synthesis was performed with 1 µg RNA (SensiFAST™ cDNA Synthesis Kit, Bioline, Cat# BIO-65053), according to the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2023Quote: ... qRT-PCR was carried out on 1:10 diluted cDNA using Sensifast Hi-Rox Sybr kit (Bioline). Two qRT-PCR reactions (technical replicates ...
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bioRxiv - Cancer Biology 2023Quote: ... and 1 µg of RNA was reverse transcribed to cDNA using SensiFAST cDNA Synthesis Kit (Bioline #65054). qPCR reactions were set up using PowerUp SYBR Green Master Mix (Applied Biosystems #A25742 ...
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bioRxiv - Cell Biology 2020Quote: ... cDNA was retrotranscribed from 0.3 µg to 1 µg using the SensiFAST cDNA Synthesis Kit (Bioline, BIO-65054). Real-time PCR was performed on the CFX384 Touch Real-time PCR Detection System (Bio-Rad ...
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bioRxiv - Genetics 2021Quote: ... 1 µg of total RNA was reverse transcribed using SensiFAST cDNA Synthesis Kit following the manufacturer’s protocol (Bioline).
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bioRxiv - Microbiology 2021Quote: ... 1:10 dilutions of RNA samples were analyzed using SensiFast SYBR Hi-ROX One-Step kit (Bioline, UK) and 400 nM primer concentrations ...
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bioRxiv - Molecular Biology 2023Quote: ... RNA was reverse-transcribed (1 mg each experimental point) by using SensiFAST cDNA Synthesis Kit (BIO-65053, Bioline) and qPCR was performed as described (18 ...
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bioRxiv - Genetics 2023Quote: ... 1 µg of RNA was used to prepare cDNA using the Sensifast cDNA synthesis kit (Bioline, London, UK). 2 µl aliquots of cDNA (corresponding to 20 ng of cDNA ...
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bioRxiv - Genetics 2021Quote: ... RNA was reverse-transcribed (1 µg each experimental point) by using SensiFAST™ cDNA Synthesis Kit (BIO-65053, Bioline) and qPCR was performed using SensiFast Sybr Lo-Rox Mix (BIO-94020 ...
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bioRxiv - Developmental Biology 2021Quote: ... cDNA was made from 1 ug total RNA using Tetro cDNA synthesis kit with DNase treatment according to manufacturer’s instructions (Bioline). cDNA was diluted into 100 ul and 2 ul of cDNA was used to perform qRT-PCR ...
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bioRxiv - Neuroscience 2022Quote: ... RT-qPCR was performed with 1/10 diluted cDNA with the SensiFAST SYBR no-rox kit (Bioline, London, UK) and Lightcycler® 480 (Roche Molecular Biochemicals ...
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bioRxiv - Plant Biology 2023Quote: ... Real-time RT-PCR was performed on 1:10 diluted cDNA using the SensiFAST SYBR No-ROX Kit (Bioline). Three biological and technical replicates were analyzed per genotype ...
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bioRxiv - Developmental Biology 2024Quote: ... 0.25– 1 µg of RNA was reverse-transcribed into cDNA using the SensiFAST cDNA Synthesis Kit (Bioline, BIO-65054). Real-time PCR was performed on the CFX384 Touch real-time PCR detection system (Bio-Rad ...
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bioRxiv - Immunology 2020Quote: ... 1 ml of Trisure (Bioline) was added and mixed ...
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bioRxiv - Microbiology 2023Quote: ... and HyperlLadder 1 kb (Bioline) was used for size determination.
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bioRxiv - Evolutionary Biology 2023Quote: ... and 1 kb Hyperladder (Bioline). PCR products were purified using Mag-Bind RXNPurePlus beads (OMEGA Biotek ...
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bioRxiv - Molecular Biology 2020Quote: ... For RT-qPCR cDNA was prepared from 1 µg of total RNA using the SensiFAST cDNA Synthesis Kit (BIO-65054, Bioline) and analysed by qPCR using the SensiFast SYBR Lo-ROX Kit (BIO-94050 ...
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bioRxiv - Evolutionary Biology 2021Quote: ... qRT-PCR was carried out on 1:10 diluted cDNA on an Applied Biosystems StepOnePlus system using Sensifast Hi-Rox Sybr kit (Bioline). Cycle conditions were as follows ...
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bioRxiv - Cell Biology 2021Quote: mRNA was isolated using NucleoSpin® RNA Plus (Machery-Nagel) and 1 µg of total mRNA was reverse-transcribed into cDNA using SensiFASTTM cDNA Synthesis Kit (Bioline) according to the manufacture’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... Isolated RNA (0.5-1 µg) was then used for reverse transcription into first strand cDNA via a SensiFast cDNA synthesis kit (Bioline). RNA spike I (TATAA ...
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bioRxiv - Evolutionary Biology 2022Quote: ... qRT-PCR was carried out on 1:2 diluted cDNA on an Applied Biosystems StepOnePlus system using a Sensifast Hi-Rox SYBR kit (Bioline). Cycle conditions were as follows ...
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bioRxiv - Evolutionary Biology 2020Quote: ... 1 mM dNTP (Bioline, Boston, MA), 250 nmol forward and reverse primers (IDT ...
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bioRxiv - Genomics 2021Quote: ... 1 × MyTaq™ Red Mix (Bioline), 10 pmoles each primer and 0.25 μL (1.25 U ...
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bioRxiv - Microbiology 2022Quote: ... with a 1 kb HyperLadder (Bioline) marker ...
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bioRxiv - Evolutionary Biology 2023Quote: ... and a 1 kbp HyperLadder (Bioline). Genomic DNA extraction from new tissue samples was conducted using the MagJet gDNA kit (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2021Quote: ... Each reaction (20 μL) consisted of 1 μL of cDNA substrate and 19 μL of a SensiFAST No-ROX Kit Master Mix (Bioline, UK). Following primer optimisation ...
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bioRxiv - Developmental Biology 2020Quote: cDNA was diluted 1:10 in nuclease-free water and subsequently applied for qRT-PCR using the SensiFast™SYBR HiROX Kit (Bioline) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... Primers were designed using NCBI primer blast (Table 3) and qRT-PCR was performed using SensiFAST™ SYBR Lo-ROX Kit (#BIO-94020, Bioline) on the BIO-RAD CFX38 Real time PCR machine ...
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bioRxiv - Neuroscience 2023Quote: ... 1 ng of the total RNA was reverse-transcribed into cDNA using a Tetro cDNA synthesis kit (BIO-65042, Bioline, London, UK). Quantitative PCR (RT-qPCR ...
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bioRxiv - Evolutionary Biology 2020Quote: ... 1 unit Biolase Taq (Bioline, Boston, MA), and 1 μl of template DNA ...
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bioRxiv - Microbiology 2019Quote: ... 1 µl of 10 mM dNTP’s (BioLine), and 0.25 µl iProof (BioRad) ...
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bioRxiv - Cell Biology 2021Quote: ... run with Hyperladder 1 DNA marker (Bioline). Selected clones were confirmed to have centrosomal localisation as expected by Airyscan confocal imaging ...