Labshake search
Citations for Bioline :
601 - 650 of 865 citations for DNA Purification kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... 2 µL 1:1 diluted cDNA and SensiFAST SYBR No-ROX Kit (#BIO-98005, Bioline). Knockdown was assessed using ΔΔCt analysis as described by Pfaffl [92].
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bioRxiv - Cancer Biology 2023Quote: ... 400 ng of total RNA was retrotranscribed to cDNA using SensiFASTTM cDNA Synthesis Kit (Bioline), according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: ... and qPCR performed using SensiFAST™ SYBR® Lo-ROX Kit following manufacturer instructions (Bioline). The qPCR reaction was performed in triplicate for each sample on QuantStudioTM 5 Real-Time PCR system with SensiFAST™ SYBR® Lo-ROX kit-compatible cycling conditions ...
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bioRxiv - Immunology 2024Quote: ... gondii 529 bp repeat region using the SensiFAST Probe No-ROX Kit (Bioline, BIO-86005) and CFX384 Real-Time System (Bio-Rad ...
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bioRxiv - Developmental Biology 2024Quote: Snap frozen cell pellets had total RNA extracted using ISOLATE II RNA mini kit (Bioline)
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bioRxiv - Plant Biology 2020Quote: The RT-qPCR experiments were carried out using SensiFAST™ SYBR No-ROX Kit (Bioline, Australia). Each reaction contains 5 µl SensiFAST mix ...
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bioRxiv - Genetics 2021Quote: ... PCR products were extracted from the agarose gel with ISOLATE II PCR and Gel Kit (Bioline) and Sanger sequenced.
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bioRxiv - Evolutionary Biology 2020Quote: ... Total RNA was extracted using a Bioline ISOLATE II RNA Plant Kit (Bioline Ltd, London, UK), following manufacturer’s instructions ...
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CRISPR-SID: identifying EZH2 as a druggable target for desmoid tumors via in vivo dependency mappingbioRxiv - Cancer Biology 2021Quote: ... Real-time quantitative PCR was performed using the SensiFAST™ SYBR® No-ROX Kit (Bioline) combined with a LightCycler® 480 Real-Time PCR System (Roche) ...
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bioRxiv - Molecular Biology 2021Quote: ... cDNA was synthesized from 1 μg of purified RNA with a SensiFast cDNA synthesis kit (Bioline). The primers used are listed in Table S2 (Supplementary Material) ...
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bioRxiv - Developmental Biology 2020Quote: ... total RNA was isolated using the ISOLATE II RNA Mini Kit (BIO-52O72, Bioline, London, UK) according to manufacturer’s instructions.
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bioRxiv - Plant Biology 2020Quote: ... Fluorescence reflecting target genes expression was determined by the SensiFAST SYBR No-ROX Kit (Bioline, Australia) using gene-specific primers (Table S1 ...
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bioRxiv - Neuroscience 2020Quote: ... 0.5-1 μg of total RNA was retrotranscribed using SensiFAST cDNA Synthesis Kit (BioLine, London, UK). Real-time PCR was performed using iTaq qPCR master mix according to manufacturer’s instructions (Bio-Rad ...
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bioRxiv - Biochemistry 2020Quote: ... 2 µg of total RNA was used to prepare cDNA with SensiFast cDNA synthesis kit (Bioline) according to the manufacturer’s instructions and diluted 1:6 with DNAse/RNAse-free water (Thermo Fisher ...
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bioRxiv - Cell Biology 2021Quote: RNA was extracted from cells seeded on hydrogels using the ISOLATE II RNA Mini Kit (Bioline) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... prat2 expression was assessed with the SensiFAST™ SYBR® Hi-ROX One-Step Kit (Bioline) according to manufacturer’s recommendations with specific primers PP25361 (Forward ...
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bioRxiv - Cell Biology 2020Quote: ... total RNA was extracted from the attached cells using an RNA Isolate II Mini Kit (BioLine) according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2020Quote: ... using TaqMan™ Gene Expression Assays (FAM-labelled) and the SensiFASTTM Probe Hi-ROX kit (Bioline). ΔCt was calculated as Ct [Target]-Geometric mean (Ct [HPRT1] ...
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bioRxiv - Plant Biology 2019Quote: ... Quantitative RT-PCR reactions were carried out using SYBR Green (SensiMix SYBR Low-ROX Kit) (BIOLINE) on 7500 MicroAmp Fast Optical 96-Well Reaction Plate (Applied Biosystems) ...
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bioRxiv - Microbiology 2020Quote: ... SARS-CoV-2 RNA was converted to cDNA using the SensiFAST cDNA kit (#BIO-65053, BioLine) with 10 μL of RNA extract per reaction following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... Quantitative RT-PCR was performed using SensiMix SYBR Low-ROX Kit (Bioline; annealing temperature – 60°C) in a Lightcycler 480 384-well plate (Roche) ...
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bioRxiv - Neuroscience 2021Quote: ... Conversion to cDNA was carried out using a SensiFAST™ cDNA synthesis kit (Bioline, London, UK). For qRT-PCR ...
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bioRxiv - Molecular Biology 2020Quote: ... 800 ng of RNA were used for reverse transcription with the SensiFASTTM cDNA Synthesis kit (Bioline) according to the standard protocol.
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bioRxiv - Molecular Biology 2021Quote: ... Schistosome cDNAs were reverse transcribed from the total RNAs using the SensiFAST cDNA Synthesis Kit (Bioline) according to the manufacturer’s instructions.
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bioRxiv - Microbiology 2021Quote: qRT-PCR was performed using the SensiFAST Probe Lo-ROX One-Step kit (Bioline Meridian, Australia) with a reaction volume of 20µl ...
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bioRxiv - Cell Biology 2021Quote: ... The cDNA (100 ng) was analysed by quantitative PCR using SensiFASTTM SYBR Lo-ROX Kit (Bioline) in the 7500 Fast Real-Time PCR System (Applied Biosystems) ...
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bioRxiv - Neuroscience 2020Quote: ... after which cDNA was synthesized using the SensiFAST(tm) cDNA Synthesis Kit (Bioline, Taunton, MA, USA).
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bioRxiv - Developmental Biology 2022Quote: RNA was isolated from cells on with the Isolate II RNA Mini Kit (Bioline, BIO-52702). 1 μg was converted to cDNA with qScript (Quanta ...
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bioRxiv - Evolutionary Biology 2022Quote: ... qPCR was performed on an Applied Biosystems StepOnePlus system using Sensifast Hi-Rox Sybr kit (Bioline) with the following PCR cycle ...
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bioRxiv - Neuroscience 2022Quote: ... after which RNA was converted to cDNA using SensiFAST™ cDNA synthesis kit (Bioline, London, UK) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2022Quote: ... frozen samples were harvested and extracted using the ISOLATE II Plant miRNA kit (Bioline, Sydney, Australia) as per the manufacturer’s instructions ...
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bioRxiv - Genomics 2023Quote: ... Two microliters of cDNA was used for subsequent amplification using SensiFAST SYBR No-Rox Kit (Bioline) on an Eco™ Real-Time PCR System (Ilumina) ...
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bioRxiv - Cancer Biology 2023Quote: ... and was reverse transcribed to cDNA using the SensiFastTM cDNA synthesis kit (Bioline, GmbH, Luckenwalde, Germany). The primers for qRT-PCR were designed using the NCBI tool Primer-BLAST–NCBI–NIH software ...
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bioRxiv - Cancer Biology 2022Quote: ... Isolated RNA was used (1 μg) to make cDNA using SensiFASTTM Kit (Bioline Cat. Bio-65053). qPCR master mix was prepared using iTaq universal SYBR green supermix (Bio-Rad Cat ...
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Quantitative Comparison of Presenilin Protein Expression Reveals Greater Activity of PS2-γ-SecretasebioRxiv - Cell Biology 2023Quote: ... RNA was extracted using ISOLATE II RNA Mini kit as per manufacturer instructions (Bioline BIO-52072) and RNA concentration and quality determined by Nanodrop (Thermo Fisher) ...
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bioRxiv - Cancer Biology 2023Quote: ... 1 mg of total RNA was retrotranscribed in cDNA using the SensiFAST cDNA Synthesis KIT (Bioline). Specific sets of primer pairs were designed and tested with primerBLAST (NCBI ...
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bioRxiv - Molecular Biology 2023Quote: ... and diluted 1:10 before quantification using the SensiFAST SYBR No-Rox kit (Bioline, BIO-98005). qRT-PCR was performed with 1 μM of each primer (a common reverse primer for both Trm1 isoforms and isoform-specific forward primers ...
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bioRxiv - Molecular Biology 2023Quote: ... cDNA was made from purified total RNA using SensiFAST™ cDNA Synthesis Kit (#BIO-65053, Bioline) as per manufacturer’s protocol ...
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bioRxiv - Genetics 2023Quote: ... The RT-qPCR reactions were set up using SensiFAST SYBR No-ROX kit (Bioline, BIO-98005) in a Light Cycler 480 II system (Roche) ...
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Increased dosage of wild-type KRAS protein drives KRAS-mutant lung tumorigenesis and drug resistancebioRxiv - Cancer Biology 2024Quote: ... 500 ng of RNA was used for reverse transcription with a Sensifast cDNA synthesis kit (Bioline). RT-qPCR was performed with LightCycler 480 SYBR Green I Master reagent with the primers listed below.
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bioRxiv - Cancer Biology 2024Quote: ... cDNA was generated from 1 µg of RNA using the SensiFAST cDNA synthesis kit (Bioline, #BIO65054). cDNA was then diluted (1:5 ...
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bioRxiv - Biochemistry 2023Quote: ... using TaqMan™ Gene Expression Assays (FAM-labelled) and the SensiFASTTM Probe Hi-ROX kit (Bioline). The following TaqMan™ Gene Expression Assays were used ...
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bioRxiv - Plant Biology 2020Quote: ... the SensiFAST SYBR No-ROX Kit was used according to manufacturer’s recommendations (Bioline Reagents Ltd., London, UK). Primers used for semi quantitative RT-PCR can be seen in Table 2.
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bioRxiv - Neuroscience 2020Quote: ... 5 µl of (2x) SYBR green master mix (SensiFAST SYBR kit, Bioline, London, United Kingdom; BIO- 94005) was used and combined with 0.4 µl of a combined forward and reverse primer mix (10 uM of forward and reverse primers in the combined master mix ...
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bioRxiv - Evolutionary Biology 2021Quote: ... qRT-PCR was performed on an Applied Biosystems StepOnePlus system using Sensifast Hi-Rox Sybr kit (Bioline) with the following PCR cycle ...
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bioRxiv - Systems Biology 2020Quote: ... Naïve and primed marker expression was determined by qPCR using the Sensifast SYBR No Rox-Kit (Bioline). Primers are listed in Supplementary Table 6.
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bioRxiv - Immunology 2019Quote: ... cDNA was generated from 500 μg of RNA using the Tetro cDNA synthesis kit (Bioline, NSW, Australia), as per the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: ... and 10-50 ng of RNA were used for reverse transcription with SensiFAST cDNA synthesis kit (Bioline) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2020Quote: ... and 10-50 ng of RNA was reverse transcribed with Sensifast cDNA synthesis kit (Bioline, London, UK). DRG axonal purity was assessed by RT-PCR ...
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bioRxiv - Cell Biology 2021Quote: ... RNA was isolated from at least 10 midguts using the Isolate II RNA Mini kit (Bioline, UK). The extracted RNA was quantified using Nanodrop (Thermo Fisher Scientific) ...