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Citations for Bioline :
1 - 50 of 136 citations for 6 OXO 9α 11α 15S TRIHYDROXY PROSTA 13E 17Z DIEN 1 OIC ACID since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2019Quote: ... [15] using MyTaqTM HS mix (Bioline, UK). Briefly ...
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bioRxiv - Microbiology 2020Quote: ... cDNA synthesis was performed with 0.25-1 µg of RNA in a 20 µL total reaction volume using a random hexamer/oligo dT strand synthesis kit as per the manufacturer’s instructions (10 min at 25°C; 15 min at 42°C; 15 min at 48°C; SensiFast, Bioline). All oligonucleotide sequences are listed in Table 1.
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bioRxiv - Microbiology 2020Quote: ... and cDNA synthesized with 0.25-1μg of RNA in a 20μL total reaction volume using a random hexamer/oligo dT strand synthesis kit in accordance with the manufacturer’s instructions (10 minutes at 25°C; 15 minutes at 42°C; 15 minutes at 48°C; SensiFast, Bioline). PCR amplification of HBV RNAs were performed using primers as previously described43 using a SYBR green real-time PCR protocol (qPCRBIO SyGreen ...
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bioRxiv - Immunology 2022Quote: ... DNA amplifications for the conTRT amplicons were performed similarly except Ranger DNA polymerase (1×10-6 approximate error/bp) from Bioline was utilized ...
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bioRxiv - Microbiology 2022Quote: ... 6 μl MyTaq Red DNA polymerase Mix (BioLine) and 1 μl of each primer (10 μmol) ...
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bioRxiv - Microbiology 2021Quote: ... 458/459/JL-D2) in TBE (Tris, boric acid, ethylenediaminetetraacetic acid) and product size approximated using HyperLadderII 50bp DNA marker (Bioline, Sydney, Australia).
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bioRxiv - Molecular Biology 2022Quote: ... 15 μl of MyTaq Red mix (Bioline, cat. no. BIO-25044) and 10 μl of 500 nM of each primer (TAC0009 and TAC0159 ...
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bioRxiv - Microbiology 2021Quote: ... the 15 μl PCR reaction mixture contained 1x MyTaq™ Red PCR Mix (Bioline, South Africa), 0.2 μM primers ...
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bioRxiv - Genomics 2023Quote: ... PCR2 was performed using 15 μL 2 x MyTaq Red Mix (Bioline, cat. no. BIO-25044) and 5 μL of each PCR1 product with well-specific Illumina PCR indexed primers (TAC0159 & TAC0009 ...
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bioRxiv - Plant Biology 2019Quote: ... The 15 μl PCR cocktail consisted of 7.5 μl of PCR MyTaq Red Mix (Bioline, GmbH, Germany), 1.0 μl of genomic DNA template (70 ng/μl) ...
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bioRxiv - Microbiology 2021Quote: ... The 15 µl PCR reaction mixture contained 1x of MyTaq ™ Red PCR Mix (Bioline, South Africa), primers at 0.2 µM ...
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bioRxiv - Microbiology 2020Quote: ... cells were collected by centrifugation at 4000 rpm for 15 minutes and pellets used for RNA extraction by RNeasy kit according to the manufacturer’s protocol (Bioline). cDNA synthesis and RT-qPCR was performed as previously described (60) ...
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bioRxiv - Evolutionary Biology 2020Quote: ... PCR reactions were carried out in a volume of 15 μL including 0.25-1.0 U of MyTaq™ HS DNA polymerase (Bioline), 2 μL of MyTaq™ 5x reaction buffer (Bioline) ...
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bioRxiv - Physiology 2022Quote: ... qPCRs were carried out in 15 μl in a 384-well plate using SensiFASTTM SYBR Hi-ROX kit (Bioline), 0·5 μM of each forward and reverse primer and 2·5 μl of template DNA ...
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bioRxiv - Microbiology 2019Quote: ... was used for preparation of cDNA-libraries which were amplified in a volume of 100 µl for 15 cycles using MyTaq (Bioline) and standard Illumina primers ...
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bioRxiv - Molecular Biology 2020Quote: ... Nucleic acids were precipitated with three volumes of ethanol in the presence of a co-precipitating agent (PINK, Bioline) and dissolved in 20 μl of milliQ-grade water.
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bioRxiv - Molecular Biology 2020Quote: ... A CCDC26 exon 6 DNA template was generated by performing a PCR using Red Mix (Bioline, Cat. No. BIO25043) WT K562 cDNA and CCDC26-specific primers that incorporated a T7 RNA polymerase promoter at the 5’-end of the DNA strand to be later transcribed (Supplementary Table 1) ...
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bioRxiv - Cancer Biology 2023Quote: ... 0.25uM forward and reverse oligo primers (see supplementary table 6) and SensiFAST SYBR Hi-ROX Kit (Bioline, BIO-92005) in 384-well plates with the LightCycler 480 Instrument II (Roche ...
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bioRxiv - Molecular Biology 2019Quote: ... Reactions were set up in total volumes of 10 µl each, containing 5× MyTaq reaction buffer (5 mM dNTPs, 15 mM MgCl2, stabilizers and enhancers) (Bioline, London, UK), 2 µM of each primer ...
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bioRxiv - Immunology 2022Quote: ... Purified nucleic acid was then immediately converted to cDNA by reverse transcription with random hexamers using the SensiFAST cDNA Synthesis Kit (Bioline Reagents, UK) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... The resulting PCR products from slurry and syringe method control samples were purified as follows: 30 μl of PCR product per sample was mixed with 6 μl 5X loading dye (Bioline, London, UK) and separated using a 1.5% agarose gel stained with SYBR Safe DNA gel stain (Invitrogen ...
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bioRxiv - Genomics 2020Quote: ... 2 □1 water and 5□1 MyTaq™ HS Mix (Bioline). RT-PCR was performed in the following conditions ...
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bioRxiv - Immunology 2020Quote: ... 1 ml of Trisure (Bioline) was added and mixed ...
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bioRxiv - Microbiology 2023Quote: ... and HyperlLadder 1 kb (Bioline) was used for size determination.
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bioRxiv - Evolutionary Biology 2023Quote: ... and 1 kb Hyperladder (Bioline). PCR products were purified using Mag-Bind RXNPurePlus beads (OMEGA Biotek ...
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bioRxiv - Molecular Biology 2023Quote: ... 2 µL 1:1 diluted cDNA and SensiFAST SYBR No-ROX Kit (#BIO-98005, Bioline). Knockdown was assessed using ΔΔCt analysis as described by Pfaffl [92].
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bioRxiv - Evolutionary Biology 2020Quote: ... 1 mM dNTP (Bioline, Boston, MA), 250 nmol forward and reverse primers (IDT ...
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bioRxiv - Genomics 2021Quote: ... 1 × MyTaq™ Red Mix (Bioline), 10 pmoles each primer and 0.25 μL (1.25 U ...
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bioRxiv - Microbiology 2022Quote: ... with a 1 kb HyperLadder (Bioline) marker ...
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bioRxiv - Evolutionary Biology 2023Quote: ... and a 1 kbp HyperLadder (Bioline). Genomic DNA extraction from new tissue samples was conducted using the MagJet gDNA kit (Thermo Fisher Scientific ...
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bioRxiv - Evolutionary Biology 2020Quote: ... 1 unit Biolase Taq (Bioline, Boston, MA), and 1 μl of template DNA ...
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bioRxiv - Microbiology 2019Quote: ... 1 µl of 10 mM dNTP’s (BioLine), and 0.25 µl iProof (BioRad) ...
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bioRxiv - Cell Biology 2021Quote: ... run with Hyperladder 1 DNA marker (Bioline). Selected clones were confirmed to have centrosomal localisation as expected by Airyscan confocal imaging ...
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bioRxiv - Molecular Biology 2020Quote: ... qPCRs were run using 2 µl cDNA (diluted 1:1) in a 10 µl reaction with 5 µl SensiFAST SBYR No-ROX Kit (Bioline) and 0.1 µM of each primer ...
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bioRxiv - Developmental Biology 2019Quote: ... PCR reactions were resolved in 1 % agarose (Bioline) gels containing 0.5 μg/ml Ethidium Bromide (Sigma ...
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bioRxiv - Cancer Biology 2020Quote: ... DNA weight marker Hyperladder™ 1-kb (BIoline) and UltraRanger 1kb (Norgen Biotek ...
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bioRxiv - Genomics 2023Quote: ... 1’ 72°C) by MyTaq Red mix (Bioline) using primers that add overhangs with EcoRI (MT024 ...
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bioRxiv - Genetics 2023Quote: ... Hyperladder 50 bp or Hyperladder 1 kb (Bioline) served as DNA fragment size markers.
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bioRxiv - Immunology 2023Quote: ... The total qPCR reaction volume was 1.5 μl and consisted of 0.5 μl of cDNA (dilution 1/12) and 1 μl of SensiFAST™ SYBR® No-ROX Kit (Bioline) containing 0.4 μM of PCR primer (Eurogenetec SA)(primer list in sup ...
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bioRxiv - Microbiology 2019Quote: ... A second round of amplification was performed by transferring 5μl of 1:100 diluted Round 1 product into 15μl Bioline MyFi™ Mix (BIOLINE GmbH, Luckenwalde, Germany) reactions.
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bioRxiv - Immunology 2023Quote: ... the aqueous phase was mixed 1:1 v/v with 70% ethanol and loaded into a column of the Isolate II RNA Mini kit (Bioline, Cincinnati, OH). Total RNA was purified following the manufacturer’s recommendations ...
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bioRxiv - Microbiology 2020Quote: ... 1 ng human genomic DNA (Bioline, Cat. # BIO-35025) was used as negative control ...
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bioRxiv - Molecular Biology 2021Quote: ... The final volume contained 1× SensiMix (Bioline, London, UK), 800 nM of each primer and 200nM of each probe and the PCR cycling conditions included an initial denaturation at 95°C for 10 minutes ...
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bioRxiv - Genomics 2023Quote: ... Samples were compared to 1 Kb hype ladder (Bioline). The primer sequences are listed in Table S9.
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bioRxiv - Cell Biology 2023Quote: ... and 1 unit of Taq polymerase (Bioline, Meridian Bioscience). The PCR conditions were an initial melting step of 94°C for 4 min ...
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bioRxiv - Microbiology 2019Quote: ... The 1 kb molecular weight marker (HyperLadder IV, Bioline, UK) was used in together with the amplified products ...
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bioRxiv - Microbiology 2020Quote: ... for 1 minute in 0.2 mL TRIsure (Bioline: BIO-38033). Homogenized tissue was brought to 1 mL volume with 0.8 mL TRIsure ...
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bioRxiv - Genetics 2020Quote: ... and 1 Unit MyTaqTM (Bioline; Meridian Bioscience Inc., London, UK) by incubation for 2 min at 96°C ...
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bioRxiv - Genomics 2023Quote: ... supplemented with 1:100 10 mg/mL Proteinase K (Bioline). TIDE was performed as described before (40) ...
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bioRxiv - Microbiology 2021Quote: ... using 5 μl product with 1 μl 5X loading dye (BioLine) to verify bands of approximately 450 bp ...