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Citations for Bioline :
251 - 300 of 393 citations for 5M Betaine Solution PCR Grade since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: Total RNA was extracted from ∼50 mg of snap frozen cryopulverised quadriceps muscle (n=6 mice per group) in 1 ml of TRIsure solution (Bioline). Following this ...
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bioRxiv - Evolutionary Biology 2021Quote: ... qRT-PCR was performed on an Applied Biosystems StepOnePlus system using Sensifast Hi-Rox Sybr kit (Bioline) with the following PCR cycle ...
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bioRxiv - Plant Biology 2020Quote: ... Quantitative Real-Time PCR was performed using a SensiFAST™ SYBR® No-ROX Kit (Bioline; www.bioline.com). Fluorescence was monitored with a CFX384 thermal cycler (Bio-Rad) ...
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bioRxiv - Microbiology 2020Quote: ... Real-time RT PCR was performed using the SensiFAST™ Probe Lo-ROX one-step kit (Bioline). In each reaction the primers final concentration was 600nM and the probe concentration was 300nM ...
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bioRxiv - Plant Biology 2021Quote: ... qRT-PCR of protonema samples was conducted using the SensiFastTM SYBR No-ROX Kit (Bioline, Luckenwalde, Germany) in a LightCycler 480 (Roche ...
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bioRxiv - Plant Biology 2020Quote: ... Table 1) were used in a pre-optimised PCR master mix (BioMix™, Bioline, Meridian Bioscience; Australia) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... Real-time RT-PCR was conducted with SensiFAST™ Probe Lo-ROX One-Step Kit (Bioline, 78005) and analyzed with the 7500 Real Time PCR System (Applied Biosystems) ...
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bioRxiv - Cell Biology 2021Quote: ... For conventional PCR we used a commercial master mix 2x My Taq HS Mix (Bioline Bio-25046). PCR amplifications were carried out in the T-100 Thermal Cycler (Biorad) ...
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bioRxiv - Cancer Biology 2022Quote: PCR reactions were performed in 25 μl reactions using the MyTaq Red Mix (Bioline, Meridian Life Science). The RT-PCR reaction conditions were optimised for each primer pair and are designated as Condition A to D ...
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bioRxiv - Microbiology 2022Quote: ... mutants were selected by PCR screening of colonies grown on LBN + 5% sucrose using Taq polymerase (Bioline) and the primers RSTX_For (5’-GTCACGGGTTGATTGATTCGCAT-3’ ...
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bioRxiv - Molecular Biology 2021Quote: ... cell lysates were diluted and 5 μl was used for genotyping PCR using MyTaq DNA polymerase (Bioline) with indicated DNA primers ...
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bioRxiv - Genetics 2022Quote: Genotyping was performed using standard PCR protocols for MyTaq HotStart Red Mix (Bioline BIO- 25048, Memphis, TN) with primers listed in Supplementary Table 7.
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bioRxiv - Molecular Biology 2020Quote: ... Both PCR reactions were carried out with 25 µl MyTaq Red mix (Bioline cat. no. BIO-25044), 0.5 µM of each primer and 50 µl final volume ...
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bioRxiv - Molecular Biology 2021Quote: ... 8 μl of DpnII-digested products was amplified by PCR with MyTaq Red Mix (Bioline #BIO-25044) and 1.25 μM primers Adr-PCR-Rand1 in a total volume of 40 μl ...
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bioRxiv - Immunology 2021Quote: ... RT-PCR was carried out using the Bioline SensiFASTTM Probe Lo-ROX Kit (Bioline, Catalogue no.-84005) and Stratagene Mx3005P (Agilent Technologies) ...
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bioRxiv - Evolutionary Biology 2022Quote: ... qRT–PCR was performed on an Applied Biosystems StepOnePlus system using Sensifast Hi-Rox Sybr kit (Bioline) with 1 µl of complementary DNA (cDNA ...
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bioRxiv - Synthetic Biology 2022Quote: ... Plasmids were generated by PCR amplification of sequences of interest with Velocity DNA Polymerase (Bioline, #BIO-21099) using primers synthesized by IDT or restriction enzyme digestion with NEB High-Fidelity enzymes ...
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bioRxiv - Microbiology 2022Quote: ... RT-PCR reaction was setup using SensiFast Probe No-ROX One- Step Kit (Bioline, Cat. BIO-76005) using the following primers/probes ...
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bioRxiv - Neuroscience 2022Quote: ... Multiplex PCR amplification was performed in a 10 μL reaction containing 1X MyTaq HS Red Mix (Bioline), 10 ng DNA template ...
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bioRxiv - Plant Biology 2023Quote: ... and real time PCR reactions were completed in duplicate for each sample using a SensiMix SYBR (Bioline); 95◻ for 10 min ...
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bioRxiv - Molecular Biology 2022Quote: ... Real- time PCR was performed using 2x SensiMix SYBR and Fluorescein Kit (Bioline, QT615-05, Luckenwalde, Germany), 20ng cDNA and pre-tested gene-specific primer sets (listed in Supplementary table 3) ...
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bioRxiv - Evolutionary Biology 2023Quote: ... qRT-PCR was carried out on 1:10 diluted cDNA using Sensifast Hi-Rox Sybr kit (Bioline). Two qRT-PCR reactions (technical replicates ...
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bioRxiv - Neuroscience 2023Quote: ... RT-PCR was performed with the Bio-Rad CFX96 cycler using the SensiFAST™ SYBR® (Bioline). Gene expression for occludin (Ocln) ...
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bioRxiv - Microbiology 2023Quote: ... both samples were subjected to PCR with human GAPDH-specific primers41 and MyTaq polymerase (Bioline, Taunton, MA). Resulting PCR amplicons were visualized by agarose gel electrophoresis ...
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bioRxiv - Microbiology 2024Quote: ... qRT-PCR analysis was performed on 50 ng of cDNA using the SensiFAST SYBR & Fluorescein kit (Bioline) with a Roche Lightcycler 96 ...
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bioRxiv - Cancer Biology 2024Quote: Quantitative RT-PCR analyses were performed in technical triplicates with SensiMix SYBR kit (Bioline, Cat# QT605–20) using the ViiA™ 7 Real Time PCR System (Life Technologies) ...
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bioRxiv - Genomics 2020Quote: ... Realtime RT-PCR was carried out on an Applied Biosystems 7900HT using SensiMix SYBR green master mix (Bioline). 300nM each primer were added to 10μl reactions in 384-well plates ...
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bioRxiv - Microbiology 2020Quote: ... The full-length genome or the nsp14-coding region were amplified by PCR using MyTaq DNA polymerase (Bioline) and after purification the PCR product was sequenced by Sanger sequencing ...
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bioRxiv - Neuroscience 2020Quote: ... Each PCR reaction (20 μL) contained 2X SensiFAST SYBR® No-ROX mix (Bioline, BIO-98005, 10 μL), 10μM primers ...
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bioRxiv - Cell Biology 2021Quote: ... Bands of correct size were cut out and purified using the Isolate II PCR and gel kit (Bioline). 50 ng of vector and 150 ng of each fragment were mixed with 3 μl of HiFi DNA Master Mix (NEB ...
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bioRxiv - Microbiology 2021Quote: ... The PCR products were analysed by 0.8% agarose-gel electrophoresis with 1 kb ladder (Bioline, Cat#H1-819101A), run at 100 V for 60 min ...
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bioRxiv - Genomics 2020Quote: ... The normalized library pools were re-amplified in 100μl PCR reactions using MyTaq (Meridian Bioline, Memphis, Tennessee, USA). For each pool ...
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bioRxiv - Microbiology 2021Quote: ... Amplicons of appropriate size were excised and purified using an Isolate II PCR and Gel kit (Bioline, UK). The purified amplicons were included in cycle sequence reactions ...
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bioRxiv - Immunology 2021Quote: ... Real time quantitative PCR (RT-qPCR) analysis was carried out using the SensiMix SYBR No-Rox kit (Bioline), using primers and conditions shown in Supplemental Table 1 ...
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bioRxiv - Cell Biology 2022Quote: ... Real-time PCR analysis was performed according to the manufacturer’s protocol (SensiMix™ SYBR® & Fluorescein Kit, Bioline) with a MyiQ Single-Color Real-Time PCR detection system (Bio-Rad ...
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bioRxiv - Genetics 2022Quote: The relative amount of PCR product was quantified by qPCR using SensiFASTTM SYBR® Hi -ROX Kit (Bioline). The sequences of primers used for the quantitative detection of the chromosomal loci are listed in Suppl Table ST6 ...
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bioRxiv - Microbiology 2022Quote: ... conventional PCR assays were replaced with qPCR assays with SensiFAST™ SYBR® No-ROX Kit (Bioline, Australia). The cycling conditions included an initial denaturation at 95°C for 3 min followed by 28 -40 cycles of 95°C for 5 sec ...
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bioRxiv - Cancer Biology 2022Quote: ... transcripts were amplified directly on RNA samples using a one-step qRT-PCR kit with SYBR green (Bioline). Primers used against DDIT3 (CHOP ...
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bioRxiv - Microbiology 2023Quote: ... The PCR products were visualised by electrophoresis in TAE buffer on a 1.5% agarose gel (Bioline, London, UK) stained with 1x SYBR Safe (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2023Quote: ... Genotyping PCR on genomic DNA was performed using the MyTaq DNA Polymerase system (Bioline, cat. no. BIO-21105). Briefly ...
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bioRxiv - Cell Biology 2023Quote: ... Quantitative real-time PCR reactions were performed with different sets of primers and Sensifast SYBR (Bioline, Taunton, MA) on a CFX96 or CFX384 Real-Time PCR Detection System (Bio-Rad ...
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bioRxiv - Molecular Biology 2024Quote: ... Correct sized amplicons were then purified using an Isolate II PCR and Gel Kit (Bioline, Meridian Biosciences, Australia) following the manufacturer’s instructions for PCR clean-up and sent for Sanger sequencing at the Victorian Clinical Genetics Services (VCGS ...
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bioRxiv - Molecular Biology 2022Quote: ... The PCR reactions were carried out using 1.5 mM MgCl2 and 1X NH4 buffers for 1U of BioTaq polymerase (Bioline), 0.2 mM dNTP mix ...
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bioRxiv - Molecular Biology 2021Quote: ... RT-PCR and RT-qPCR were performed according to the manufacturer’s protocols using MyTaq™ Red Mix (Bioline/BIOCAT) for RT-PCR and GoTaq qPCR Master Mix (Promega ...
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bioRxiv - Plant Biology 2020Quote: ... Real-time PCR reactions were performed in a 384-well microlitre using SensiFAST SYBR® No-ROX Kit (Bioline). The primers used here were designed using the open-source program QuantPrime-qPCR primer designed tool (Arvidsson et al. ...
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bioRxiv - Molecular Biology 2020Quote: ... The genomic regions flanking the nuclease target sites were PCR amplified using MyTaq™ DNA Polymerase (Bioline, https://www.bioline.com/) and primers listed on Table S3 ...
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bioRxiv - Microbiology 2021Quote: ... 2μL were used as template for genomic PCRs in a 50 μL reaction with Velocity DNA Polymerase (Bioline#21098) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... A CCDC26 exon 6 DNA template was generated by performing a PCR using Red Mix (Bioline, Cat. No. BIO25043) WT K562 cDNA and CCDC26-specific primers that incorporated a T7 RNA polymerase promoter at the 5’-end of the DNA strand to be later transcribed (Supplementary Table 1) ...
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bioRxiv - Cancer Biology 2021Quote: ... The relative amount of mRNA transcripts was measured by real-time PCR using SensiFAST SYBR N-ROX Kit (Bioline) and calculated by Bio-Rad CFX Manager Software ...
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bioRxiv - Microbiology 2023Quote: ... The qRT-PCR reaction was performed using 35 μL SensiFAST™ Probe LoROX One-Step Kit (Bioline, Taunton, MA) and 15 μL of extracted eluate ...