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Citations for Bioline :
151 - 195 of 195 citations for Nonanoic acid reaction products with diethanolamine since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: For each reaction 1μL of gDNA solution was suspended in a PCR tube with 2μL of MyTaq red reaction buffer (5x; Bioline), 1µL of pooled primers/oligonucleotides (final concentration of each primer 10μM or 5μM for Ubc-Cre) ...
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bioRxiv - Neuroscience 2024Quote: ... 96-well plates were loaded with 15μl reaction mixture (1:25 primers, 1:3 cDNA and 1:2 SYBR-Green SensiMix (Bioline)) ...
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bioRxiv - Developmental Biology 2024Quote: ... qPCRs were performed using 10 µl reactions consisting of 5 μl SensiFAST SBYR No-ROX Kit (#BIO-98005, Bioline), 0.2 μM of each primer and 2 μl of relevant cDNA (or H2O in the case of no template controls) ...
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bioRxiv - Molecular Biology 2020Quote: ... Nucleic acids were precipitated with three volumes of ethanol in the presence of a co-precipitating agent (PINK, Bioline) and dissolved in 20 μl of milliQ-grade water.
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bioRxiv - Microbiology 2021Quote: ... The resulting PCR products from slurry and syringe method control samples were purified as follows: 30 μl of PCR product per sample was mixed with 6 μl 5X loading dye (Bioline, London, UK) and separated using a 1.5% agarose gel stained with SYBR Safe DNA gel stain (Invitrogen ...
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bioRxiv - Developmental Biology 2020Quote: ... one μg of each template was synthesized into cDNA with a standard reaction mix (SensiFAST™ cDNA Synthesis Kit, Bioline) in a thermal cycler (Bio-Rad T100TM Thermal Cycler ...
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bioRxiv - Neuroscience 2021Quote: Genomic DNA extraction from tail snips and genotyping PCR reactions were performed using MyTaq Extract-PCR kit (Bioline, Taunton, MA). All the mice were genotyped by the fragment PCR method as indicated in the Jackson Laboratory website ...
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bioRxiv - Molecular Biology 2021Quote: ... a 10 μl qPCR reaction is set up using SensiFAST SYBR No-ROX kit (FroggaBio/Bioline, catalogue number BIO-98050), forward and reverse primers (Table 2) ...
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bioRxiv - Neuroscience 2021Quote: ... cDNA samples were run in triplicate in 96 well reaction plates using SYBR-Green-based qPCR (SensiFast, HI-ROX, Bioline) according to manufacturer’s instructions using a StepOnePlus System (Applied Biosystems ...
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bioRxiv - Plant Biology 2020Quote: ... Quantitative real-time PCR reactions using cDNA samples as template were carried out using a SensiFAST SYBR No-ROX Kit (Bioline) in a CFX96 Touch (BIO-RAD ...
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bioRxiv - Physiology 2020Quote: ... Genomic DNA extraction from tails snips and genotyping PCR reactions were performed using MyTaq Extract-PCR kit (Bioline, Taunton, MA). Mice were genotyped using a fragment PCR method with the primers shown in the table below and indicated on the Jackson Laboratory website ...
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bioRxiv - Microbiology 2020Quote: ... Real-time PCR reactions were performed in 12 μL mixtures containing 1 x SensiFAST SYBR No-ROX mix (Bioline, Australia), 400 nM of each forward and reverse primer (Table 3 ...
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bioRxiv - Cancer Biology 2021Quote: ... and qPCR reactions were conducted using gene-specific primers with SensiFAST SYBR No-ROX kit (Bioline, #Cat no: BIO-98005) as described previously (Sibai et al. ...
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bioRxiv - Molecular Biology 2022Quote: RT-qPCR was performed in 20.0 µl final reaction volume containing 10.0 µl of 2X SensiMix SYBR® No-ROX master mix (Bioline, UK), 0.3 µM of forward and reverse primers and 1.0 µl of cDNA ...
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bioRxiv - Molecular Biology 2020Quote: ... qPCRs were run using 2 µl cDNA (diluted 1:1) in a 10 µl reaction with 5 µl SensiFAST SBYR No-ROX Kit (Bioline) and 0.1 µM of each primer ...
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bioRxiv - Molecular Biology 2020Quote: ... and 1 μL of DNA was used in a PCR reaction using 0.3 μΜ primers and 5U of MyTaq DNA polymerase (Bioline).
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bioRxiv - Cell Biology 2023Quote: ... and used as a template for quantitative polymerase chain reaction with reverse transcription (qRT–PCR) analysis using SensiFAST SYBR Lo-ROX Kit (Bioline). Samples were analyzed using a QuantStudio 3 Real-Time PCR System (ThermoFisher) ...
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bioRxiv - Microbiology 2023Quote: ... was performed in technical triplicate with 50 ng of cDNA per reaction using the SensiFAST SYBR & Fluorescein Kit from Bioline, on a Roche Lightcycler 96 ...
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bioRxiv - Microbiology 2023Quote: ... The reactions (10 μL total volume) contained 5 μL of 2 × SensiFAST™ SYBR No-ROX Kit (Bioline, London, UK), 0.2 μM of each arsM gene primer ...
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bioRxiv - Molecular Biology 2022Quote: ... PCR amplification was performed in a total reaction mixture of 10 μL containing 1x SensiFast master mix (Bioline, Meridian Bioscience), 0.5 μM of reverse and forward primers ...
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bioRxiv - Cell Biology 2022Quote: ... The qRT-PCR reaction was performed using 35 µL SensiFAST™ Probe Lo- ROX One-Step Kit (Bioline, Taunton, MA) and 15 µL of extracted eluate ...
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bioRxiv - Plant Biology 2024Quote: ... 2 μL of purified genomic DNA (50 ng μL-1) were used directly in 10 μL reaction volumes containing 5 μL of SensiFAST HRM mix (Bioline), 1.6 μL sterile distilled water and 0.7 μL of each primer (100 nM) ...
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bioRxiv - Neuroscience 2020Quote: ... RT-PCR reactions were set up in a 384-well format using 2X SensiFAST Probe No-ROX Kit (Bioline, BIO-86005) and 1 µl of cDNA per reaction in a total volume of 10 µl ...
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bioRxiv - Microbiology 2022Quote: ... A few microliters of each PCR product were run on an agarose gel to assess the success of the PCR reaction and the remains cleaned through an Isolate II PCR and Gel kit (Bioline, USA) and sent for sequencing with primer C1-N-2191 ...
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bioRxiv - Neuroscience 2021Quote: ... qRT-PCR reactions were performed in triplicate using a Corbett Rotorgene 6000 Real-Time PCR machine with Sensimix SYBR No-Rox (Bioline, UK) and intron-spanning primers (Table S1) ...
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bioRxiv - Neuroscience 2021Quote: ... We synthesized complementary DNA (cDNA) from 100ng of RNA in a 40μl reaction containing 100 units of Tetro Reverse transcriptase (Bioline, Taunton, MA), 8μl of 5X RT buffer ...
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bioRxiv - Developmental Biology 2021Quote: ... and 7μl of reaction mix containing primers (forward and reverse) at a final concentration of 300nM and Sensifast SYBR®Low-ROX Mix (Bioline). qPCR assays were performed on a Mic qPCR instrument (Bio Molecular Systems ...
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bioRxiv - Microbiology 2020Quote: ... cDNA was then synthesized from 2.5 μg RNA in a 20 μL reaction using both random hexamers and oligo(dT) primers provided with the Tetro cDNA Synthesis kit (Bioline™). Ten defense genes were quantified using the SYBR Green qRT-PCR kit on a ViiA™ 7 sequence detection system ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... We synthesized complementary DNA (cDNA) from 100 ng of RNA in a 40µl reaction containing 100 units of Tetro Reverse transcriptase (Bioline, Taunton, MA), 8µl of 5X RT buffer ...
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bioRxiv - Genomics 2023Quote: ... The PCR product was cut from gel to remove any other undesired products and cleaned with the PCR Isolate II PCR and Gel Kit (Bioline, cat. no. BIO-52060). The isolated samples were sequenced by Illumina MiSeq for screen 1 (14.3 million reads ...
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bioRxiv - Microbiology 2020Quote: ... 250-1,000 ng of extracted RNA was converted to cDNA in a reaction volume of 20 μl using the Tetro cDNA synthesis kit (BIO-65043, Bioline, London, UK). The cDNA was then amplified using an SYBR green RT-PCR kit (06924204001 ...
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bioRxiv - Neuroscience 2020Quote: ... cDNA was prepared from 300 ng of RNA in a total reaction volume of 20 µl using the Sensi-FAST cDNA synthesis kit (Bioline, BIO-65053). RT-PCR reactions were set up in a 384-well format using 2X SensiFAST Probe No-ROX Kit (Bioline ...
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bioRxiv - Cancer Biology 2020Quote: Real-time RT-PCR reactions were performed in 20ml volumes with 10 ml of SensiFAST SYBR Lo-ROX Kit (Bioline, Taunton, MA) 2ml of cDNA template and 0.5ml each of the forward and reverse primers of the gene of interest (GOI) ...
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bioRxiv - Cell Biology 2020Quote: ... 25 ng cDNA were used in a total reaction mix of 20 μl containing 10 ul Sensi Fast SYBR Green Hi-Rox (Bioline, London, UK) as well as 400 nM forward and reverse primer (Supplementary Material and Methods ...
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bioRxiv - Microbiology 2020Quote: All PCRs were carried out in 25 μl reaction volumes containing 0.05 units of MyTaq™ DNA Polymerase (Bioline Laboratories, London, UK), 1x MyTaq™ Mix (Bioline ...
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bioRxiv - Microbiology 2023Quote: ... SdhC and SdhD was performed with primers listed in Table 2 and the following reaction conditions: PCR amplification used MyTaq DNA Polymerase (Bioline, London, UK), with 0.25 μM each forward and reverse primer ...
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bioRxiv - Immunology 2022Quote: ... Purified nucleic acid was then immediately converted to cDNA by reverse transcription with random hexamers using the SensiFAST cDNA Synthesis Kit (Bioline Reagents, UK) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... Amplification was carried out in duplicate in 25 µL reactions prepared with 1X SensiFAST™ SYBR® Lo-ROX Kit (Bioline, Meridian Bioscience), 0.4 µL of the forward and reverse primer (IDT ...
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bioRxiv - Neuroscience 2022Quote: ... Samples were prepared for qPCR in technical triplicates in 5 µL reaction volumes using SensiFAST SYBR Lo-ROX 2X master mix (Bioline; Cat. No. BIO-94005), custom qPCR primers from Integrated DNA Technologies used at a final concentration of 0.2 µM ...
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bioRxiv - Microbiology 2021Quote: The origin of viral genes present in samples were determined by gene-specific reverse transcription polymerase chain reaction (RT-PCR) using a SensiFast Probe No-ROX one-step reverse transcription kit (Bioline, Meridian Biosciences, Ohio, USA). Each 20 µL reaction contained 5 μl of virus sample in 0.05% Triton-X 100 ...
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bioRxiv - Genomics 2020Quote: ... 10 μl of each of the 96 Libraries were separately amplified in 20 μl PCR reactions using MyTaq™ (Meridian Bioline, Memphis, Tennessee, USA) and standard Illumina TrueSeq amplification primers (Illumina ...
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bioRxiv - Neuroscience 2024Quote: ... Samples were prepared for qPCR in technical triplicates in 10-µl reaction volumes using SensiFAST SYBR Lo-ROX 2X Master Mix (Bioline; Cat. No. BIO-94005), custom qPCR primers from Integrated DNA Technologies used at a final concentration of 0.2LµM and cDNA diluted at 1:3 ...
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bioRxiv - Microbiology 2020Quote: ... 2uL of the DNA extracted from sputum samples was used as a template in 20-mL qPCR reactions containing 1× SensiFAST™ Probe Hi-ROX (Bioline, Cat. No. BIO-82020), 200 nM probe ...
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bioRxiv - Microbiology 2022Quote: ... 2 μL was used as a template in 20 μL qPCR reactions containing 10 μL SensiFAST™ Probe Hi-ROX (Bioline, Cat. No. BIO-82020), 200 nM probe ...
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bioRxiv - Cell Biology 2023Quote: ... Cas9 target sites within the AAVS1 locus were PCR amplified in a 25 or 50 μL reaction volume per sample using high-performance RANGER PCR Mix (Bioline USA, Inc., Memphis, TN, USA) or PlatinumTM SuperFiTM PCR Master Mix (Thermo Fisher Scientific ...