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Citations for Bioline :
51 - 94 of 94 citations for CTLA 4 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... Human genomic DNA purchased from Bioline Australia (Cat No ...
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bioRxiv - Evolutionary Biology 2021Quote: ... qRT-PCR was performed on an Applied Biosystems StepOnePlus system using Sensifast Hi-Rox Sybr kit (Bioline) with the following PCR cycle ...
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bioRxiv - Cell Biology 2021Quote: ... RT-qPCR was performed using SensiFAST™ SYBR® Hi-ROX Kit (Bioline, cat. no. BIO-92020) in a 96-well white plate (Roche ...
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bioRxiv - Evolutionary Biology 2022Quote: ... qRT–PCR was performed on an Applied Biosystems StepOnePlus system using Sensifast Hi-Rox Sybr kit (Bioline) with 1 µl of complementary DNA (cDNA ...
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bioRxiv - Evolutionary Biology 2023Quote: ... qRT-PCR was carried out on 1:10 diluted cDNA using Sensifast Hi-Rox Sybr kit (Bioline). Two qRT-PCR reactions (technical replicates ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Quantitative analysis of the ERBB2 was performed using a SensiFAST™ SYBR Hi-ROX kit (Bioline, Canada). PCR amplification condition was applied as described previously [29] ...
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bioRxiv - Molecular Biology 2021Quote: ... qPCR quantification of DNA samples was carried out using the SensiFAST SYBR Hi-ROX Kit (Bioline, BIO-92005) in 96-well plates using a ABI PRISM 7000 system (Applied Biosystems) ...
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bioRxiv - Molecular Biology 2019Quote: ... Real-time PCRs were analyzed by SensiFAST™ SYBR® Hi-ROX Kit (BIO-92020, Bioline, London, UK). A PCR program was used as described ...
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bioRxiv - Microbiology 2021Quote: ... 1:10 dilutions of RNA samples were analyzed using SensiFast SYBR Hi-ROX One-Step kit (Bioline, UK) and 400 nM primer concentrations ...
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bioRxiv - Developmental Biology 2019Quote: ... 2.5 ng cDNA was amplified in a 10 μl reaction containing 1x Sensimix Sybr Hi-Rox reagents (Bioline) and 375 nM of both forward and reverse primer (Supplementary Table S1) ...
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bioRxiv - Developmental Biology 2019Quote: ... 20 ng DNA was used in a 10 μl reaction containing 1x Sensimix Sybr Hi-Rox reagents (Bioline) and 375 nM of both forward and reverse primer (Supplementary Table S1) ...
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bioRxiv - Genetics 2022Quote: The relative amount of PCR product was quantified by qPCR using SensiFASTTM SYBR® Hi -ROX Kit (Bioline). The sequences of primers used for the quantitative detection of the chromosomal loci are listed in Suppl Table ST6 ...
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bioRxiv - Genetics 2020Quote: ... 4 mM MgCl2 (Bioline); 800nM dNTPs (Bioline) ...
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bioRxiv - Physiology 2022Quote: ... qPCRs were carried out in 15 μl in a 384-well plate using SensiFASTTM SYBR Hi-ROX kit (Bioline), 0·5 μM of each forward and reverse primer and 2·5 μl of template DNA ...
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bioRxiv - Cancer Biology 2023Quote: ... 0.25uM forward and reverse oligo primers (see supplementary table 6) and SensiFAST SYBR Hi-ROX Kit (Bioline, BIO-92005) in 384-well plates with the LightCycler 480 Instrument II (Roche ...
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bioRxiv - Neuroscience 2023Quote: ... Real-time RT-PCR was performed using a SensiFastTM SYBR® Hi-ROX kit (#BIO-92020; Bioline USA Inc.) in an Applied Biosystems QuantStudio 7 Flex Real-Time PCR system (Thermo Fisher Scientific Inc.) ...
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bioRxiv - Evolutionary Biology 2020Quote: ... We labeled each library with a sample-specific pair of barcodes and combined libraries in equimolar ratios based on qPCR using SensiFAST SYBR Hi-ROX master mix (Bioline), then sequenced the combined pools on four lanes of 50bp single end reads (Illumina HiSeq2500 ...
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bioRxiv - Evolutionary Biology 2021Quote: ... qRT-PCR was carried out on 1:10 diluted cDNA on an Applied Biosystems StepOnePlus system using Sensifast Hi-Rox Sybr kit (Bioline). Cycle conditions were as follows ...
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bioRxiv - Neuroscience 2021Quote: ... cDNA samples were run in triplicate in 96 well reaction plates using SYBR-Green-based qPCR (SensiFast, HI-ROX, Bioline) according to manufacturer’s instructions using a StepOnePlus System (Applied Biosystems ...
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bioRxiv - Neuroscience 2020Quote: ... The cDNA products were diluted 10 times and used for qPCR using a StepOnePlusTM instrument (Applied Biosystem, USA) and SensiFAST SYBR Hi-ROX Kit (Bioline) following the manufacturer’s guidelines ...
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bioRxiv - Microbiology 2021Quote: qRT-PCR was used to quantify transcript levels of specific quorum sensing-controlled genes in DS40M4 and was performed using the SensiFast SYBR Hi-ROX One-Step Kit (Bioline) according to the manufacturer’s guidelines ...
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bioRxiv - Microbiology 2021Quote: Real-time qRT-PCR (quantitative reverse transcription PCR) was used to quantify transcript levels using the SensiFast SYBR Hi-ROX One-Step Kit (Bioline) according to the manufacturer’s guidelines ...
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bioRxiv - Neuroscience 2021Quote: ... The cDNA was then diluted and used as template for qPCR, which was performed with a StepOnePlus instrument (Applied Biosystem, USA) and SensiFAST SYBR Hi-ROX Kit (Bioline) according to the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2022Quote: qPCR was performed on each of the samples using an Applied Biosystems StepOnePlus system with a Sensifast Hi-Rox Sybr kit (Bioline). Cycle conditions were as follows ...
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bioRxiv - Evolutionary Biology 2022Quote: ... qRT-PCR was carried out on 1:2 diluted cDNA on an Applied Biosystems StepOnePlus system using a Sensifast Hi-Rox SYBR kit (Bioline). Cycle conditions were as follows ...
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bioRxiv - Microbiology 2020Quote: ... 1 ng human genomic DNA (Bioline, Cat. # BIO-35025) was used as negative control ...
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bioRxiv - Neuroscience 2019Quote: ... The cDNA products were then diluted 10 times and applied for qPCR using a StepOnePlus™ instrument (Applied Biosystem, USA) and SensiFAST SYBR Hi-ROX Kit (Bioline) followed the protocol from the manufacturer ...
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bioRxiv - Plant Biology 2020Quote: ... Quantitative real-time RT–PCR (qRT–PCR) was carried out using the SensiFast™SYBR Hi-ROX One-Step Kit (Bioline) as described (Wobbe et al. ...
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bioRxiv - Biochemistry 2021Quote: ... Quantitation of the OP91 transcript was done by quantitative PCR using the SensiFAST SYBR® Hi-ROX Kit (Bioline, Memphis, TN, USA) and the StepOnePlus™ Real-Time PCR detection system (Applied biosystems by Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2020Quote: ... 25 ng cDNA were used in a total reaction mix of 20 μl containing 10 ul Sensi Fast SYBR Green Hi-Rox (Bioline, London, UK) as well as 400 nM forward and reverse primer (Supplementary Material and Methods ...
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bioRxiv - Immunology 2023Quote: ... Quantitative PCRs (qPCRs) were performed on cDNA samples using SensiFAST™ SYBR® Hi-ROX or SensiFAST™ Probe No-ROX kits (Bioline) on a StepOnePlus real-time PCR apparatus (Applied Biosystems) ...
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bioRxiv - Microbiology 2020Quote: ... 2uL of the DNA extracted from sputum samples was used as a template in 20-mL qPCR reactions containing 1× SensiFAST™ Probe Hi-ROX (Bioline, Cat. No. BIO-82020), 200 nM probe ...
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bioRxiv - Microbiology 2022Quote: ... 2 μL was used as a template in 20 μL qPCR reactions containing 10 μL SensiFAST™ Probe Hi-ROX (Bioline, Cat. No. BIO-82020), 200 nM probe ...
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bioRxiv - Molecular Biology 2021Quote: ... 4 μl of 5X Mytaq reaction buffer (Bioline, London, UK), 0.2 μl of MyTaq DNA polymerase (Bioline ...
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bioRxiv - Molecular Biology 2020Quote: ... and 4 μl of 5X MyTaq reaction buffer (Bioline, London, UK). The amplification products were analyzed by electrophoresis using a 2% agarose gel.
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bioRxiv - Microbiology 2022Quote: ... Quantification of SFB in human tissue biopsies was performed using the MasterMix SensiFAST™ SYBR (BIOLINE) and 3 different primer sets ...
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bioRxiv - Microbiology 2023Quote: ... both samples were subjected to PCR with human GAPDH-specific primers41 and MyTaq polymerase (Bioline, Taunton, MA). Resulting PCR amplicons were visualized by agarose gel electrophoresis ...
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bioRxiv - Plant Biology 2022Quote: ... then resuspended using the vortex in 4 mL of Trisure (Bioline Iberia, Spain) and the lysis was performed after incubation for 5 min at room temperature ...
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bioRxiv - Microbiology 2019Quote: Plasmodium screening of human subjects was done in the regions surrounding Mbita using RDT kits (SD Bioline, UK). Microscopy was carried out on RDT-positive samples to confirm the presence of P ...
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bioRxiv - Molecular Biology 2020Quote: ... Stain was also added to the markers Hyperladder 1kb™ and Hyperladder 4™ (Bioline) to give final concentrations of 10x and 100x.
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bioRxiv - Genetics 2020Quote: Separation of the amplified product was accomplished in a 4% (w/v) agarose (Bioline, London) gel in 1% (w/v ...
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bioRxiv - Cell Biology 2020Quote: ... Tissue pieces were digested at 4 °C on an orbital shaker-incubator at 200 × rpm (Bioline Global, New South Wales, Australia). After overnight digestion ...
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bioRxiv - Pathology 2024Quote: ... then incubated overnight at 37°C with staining buffer (5-bromo-4-chloro-3-indolyl β-D-galactopyranoside [X-gal, Bioline, London ...
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bioRxiv - Molecular Biology 2024Quote: ... the Methyl Indexed PCR was performed by mixing 4 µL of ligated DNA with x-Gen Dual combinatorial Indexes (IDT) (125 nM final concentration) and MyTaq RedMix (Bioline, BIO-25048) in a final volume of 40 µL ...