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Citations for Bioline :
851 - 900 of 948 citations for Onion Yellow Dwarf Virus OYDV PCR Kits since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2022Quote: ... supplemented with 100 μg/ml ampicillin were processed with either the ISOLATE II Plasmid Mini Kit (Bioline, #BIO-52057) for sequence verification ...
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bioRxiv - Physiology 2022Quote: ... qPCRs were carried out in 15 μl in a 384-well plate using SensiFASTTM SYBR Hi-ROX kit (Bioline), 0·5 μM of each forward and reverse primer and 2·5 μl of template DNA ...
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bioRxiv - Genomics 2022Quote: ... 24 samples) were pooled and used for generation of full length cDNA using the Tetro cDNA synthesis kit (Bioline) according to manufacturer’s instructions and using only the oligo-dT primer ...
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bioRxiv - Neuroscience 2022Quote: ... RT-qPCR was performed with 1/10 diluted cDNA with the SensiFAST SYBR no-rox kit (Bioline, London, UK) and Lightcycler® 480 (Roche Molecular Biochemicals ...
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bioRxiv - Developmental Biology 2024Quote: ... 0.25– 1 µg of RNA was reverse-transcribed into cDNA using the SensiFAST cDNA Synthesis Kit (Bioline, BIO-65054). Real-time PCR was performed on the CFX384 Touch real-time PCR detection system (Bio-Rad ...
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bioRxiv - Microbiology 2023Quote: ... and a sample was taken for DNA extraction and purification using an Isolate II Genomic DNA purification kit (Bioline). Also ...
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bioRxiv - Cancer Biology 2023Quote: ... 0.25uM forward and reverse oligo primers (see supplementary table 6) and SensiFAST SYBR Hi-ROX Kit (Bioline, BIO-92005) in 384-well plates with the LightCycler 480 Instrument II (Roche ...
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bioRxiv - Molecular Biology 2023Quote: ... 4.2 µL purified DNA from chromatin immunoprecipitation was mixed with 5 µL 2x SensiFAST SYBR No-ROX kit (Bioline) and 0.4 µL forward and reverse primer (10 µM) ...
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bioRxiv - Genetics 2023Quote: RNA was isolated from cells with Isolate II RNA Mini Kit according to the manufacturer’s instructions (Bioline, BIO-52702). 1 μg of RNA was converted to cDNA using qScript (Quanta ...
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bioRxiv - Microbiology 2023Quote: ... the pellet was suspended in 100 μl of preheated elution buffer G (ISOLATE II Genomic DNA kit, Bioline Meridian). The DNA quality and quantity was checked by Nanodrop spectrophotometer (Thermo Scientific ...
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bioRxiv - Biochemistry 2023Quote: ... 1μg of RNA was used in a 20-μl reaction mixture by using a SensiFast cDNA synthesis kit (Bioline). For real-time PCR analysis ...
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bioRxiv - Cell Biology 2024Quote: ... These library pools were further qPCR-based quantified using the JetSeq Library Quantification Lo-ROX kit (BIO-68029, Bioline) according to the manufacturer’s guidelines ...
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bioRxiv - Molecular Biology 2024Quote: ... gDNA was extracted from the unsorted control cell pools using the ISOLATE II Genomic DNA Kit (BIO-52067, Bioline).
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bioRxiv - Cancer Biology 2024Quote: Cells were treated with 1µM and 10µM 4NQO for 6 hours and total RNA was extracted with Isolate II RNA mini kit (Bioline) following the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2024Quote: ... qPCRs were performed using 10 µl reactions consisting of 5 μl SensiFAST SBYR No-ROX Kit (#BIO-98005, Bioline), 0.2 μM of each primer and 2 μl of relevant cDNA (or H2O in the case of no template controls) ...
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bioRxiv - Evolutionary Biology 2020Quote: ... For each gene a total of 8 clones from each plant were isolated from overnight LB cultures using an ISOLATE II Plasmid Mini Kit (Bioline) prior to sequencing with universal M13F and M13R primers by GATC Biotech (Konstanz ...
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bioRxiv - Developmental Biology 2020Quote: ... one μg of each template was synthesized into cDNA with a standard reaction mix (SensiFAST™ cDNA Synthesis Kit, Bioline) in a thermal cycler (Bio-Rad T100TM Thermal Cycler ...
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bioRxiv - Genomics 2020Quote: ... Pluripotency and early differentiation marker as well as selected known imprinted genes expression was determined by qPCR using the Sensifast SYBR No Rox-Kit (Bioline).
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bioRxiv - Molecular Biology 2020Quote: ... For RT-qPCR cDNA was prepared from 1 µg of total RNA using the SensiFAST cDNA Synthesis Kit (BIO-65054, Bioline) and analysed by qPCR using the SensiFast SYBR Lo-ROX Kit (BIO-94050 ...
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bioRxiv - Molecular Biology 2021Quote: ... and lysozyme were determined with a Real Time Thermal cycler qTower (Analytical Jena) system using a SensiFast SYBR kit (Bioline) according to the supplier’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... a 10 μl qPCR reaction is set up using SensiFAST SYBR No-ROX kit (FroggaBio/Bioline, catalogue number BIO-98050), forward and reverse primers (Table 2) ...
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bioRxiv - Molecular Biology 2021Quote: ... The purified RNA was then reverse transcribed into complementary DNA (cDNA) with the SensiFAST™ complementary cDNA synthesis Kit (Bioline) and used in qPCR ...
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Genomic and phenotypic characterization of finger millet indicates a complex diversification historybioRxiv - Genetics 2021Quote: ... Genomic DNA (gDNA) was extracted from finely-ground leaf material using the ISOLATE II Genomic DNA Kit (Bioline Pty Ltd) and according to the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2021Quote: ... qRT-PCR was carried out on 1:10 diluted cDNA on an Applied Biosystems StepOnePlus system using Sensifast Hi-Rox Sybr kit (Bioline). Cycle conditions were as follows ...
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bioRxiv - Neuroscience 2020Quote: ... The cDNA products were diluted 10 times and used for qPCR using a StepOnePlusTM instrument (Applied Biosystem, USA) and SensiFAST SYBR Hi-ROX Kit (Bioline) following the manufacturer’s guidelines ...
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bioRxiv - Microbiology 2021Quote: RNA from GP barley infected with Fg-IFA65 at 5 dpi and an uninfected control was extracted with the Isolate II plant miRNA kit (Bioline). 1 µg of RNA (>200 nt ...
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bioRxiv - Microbiology 2021Quote: qRT-PCR was used to quantify transcript levels of specific quorum sensing-controlled genes in DS40M4 and was performed using the SensiFast SYBR Hi-ROX One-Step Kit (Bioline) according to the manufacturer’s guidelines ...
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bioRxiv - Cancer Biology 2021Quote: ... and qPCR reactions were conducted using gene-specific primers with SensiFAST SYBR No-ROX kit (Bioline, #Cat no: BIO-98005) as described previously (Sibai et al. ...
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bioRxiv - Immunology 2022Quote: ... Complementary DNA was synthesized using 2 µg of RNA and the Tetro cDNA synthesis kit (Bioline Reagents Ltd., London, UK) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... Extracted RNA was subsequently assayed for a short fragment of the influenza A matrix gene (Spackman et al. 2002) using the SensiFAST Probe Lo-Rox qPCR Kit (Bioline). A cycle threshold (Ct ...
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bioRxiv - Developmental Biology 2021Quote: ... washed in water and 100% ethanol prior to RNA extraction using the ISOLATE II RNA Mini Kit’s protocol (Bioline, UK). The extracted RNA was quantified using Nanodrop (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2020Quote: ... qPCRs were run using 2 µl cDNA (diluted 1:1) in a 10 µl reaction with 5 µl SensiFAST SBYR No-ROX Kit (Bioline) and 0.1 µM of each primer ...
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bioRxiv - Immunology 2021Quote: ... Complementary DNA was synthesized using 2 μg of RNA and the Tetro cDNA synthesis kit (Bioline Reagents Ltd., London, UK) according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... The cDNA was then diluted and used as template for qPCR, which was performed with a StepOnePlus instrument (Applied Biosystem, USA) and SensiFAST SYBR Hi-ROX Kit (Bioline) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: Real-Time PCR analysis was performed using TaqMan gene expression assays using primers (see Resources Table) and SensiFast Probe Lo-ROX Kit (cat.# BIO-84020, Bioline) following manufacturer’s recommendation ...
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bioRxiv - Cell Biology 2021Quote: mRNA was isolated using NucleoSpin® RNA Plus (Machery-Nagel) and 1 µg of total mRNA was reverse-transcribed into cDNA using SensiFASTTM cDNA Synthesis Kit (Bioline) according to the manufacture’s protocol ...
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bioRxiv - Genetics 2022Quote: ... 1 μg of RNA was then reverse transcribed with random hexamer and anchored oligo dT primers using SensiFast cDNA synthesis kit (Bioline). Quantitative PCR was performed on the diluted cDNA using SensiFast probe (Bioline ...
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bioRxiv - Genetics 2022Quote: The frequency of proximity ligated fragments containing the Myc promoter and other downstream regions was then quantified on the BioRad C1000 Thermo cycler using the SensiMix SYBR NOROX kit (Bioline) with 60°C annealing temperature ...
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bioRxiv - Molecular Biology 2022Quote: ... ChIP DNA and input DNA were diluted 5X and amplified using 500 nM primers and SensiFAST SYBR (No-ROX Kit, Bioline) using a LightCycler 480 (Roche).
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bioRxiv - Microbiology 2022Quote: ... 500-ng portions of DNA extracted from each larva were subjected to qPCR using the SensiMic SybR Green kit (Bioline). Forward (5′-ACTTCCGCAATGGACGTTAC-3′ ...
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bioRxiv - Neuroscience 2024Quote: ... 150 ng of total RNA was converted to complementary DNA (cDNA) using SensiFASTTM cDNA synthesis kit following manufacturer instructions (Bioline) and qPCR performed in technical triplicate using SensiFAST™ SYBR® Lo-ROX Kit following manufacturer instructions (Bioline ...
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bioRxiv - Microbiology 2023Quote: ... The reactions (10 μL total volume) contained 5 μL of 2 × SensiFAST™ SYBR No-ROX Kit (Bioline, London, UK), 0.2 μM of each arsM gene primer ...
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bioRxiv - Cancer Biology 2023Quote: ... 1 μl of the dilution was mixed with 0.41 μM primers (Supplemental Table S4) and 1x Sensifast SYBR non-Rox kit (Bioline Bio98005). The real-time PCR program cycled as follows ...
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bioRxiv - Cancer Biology 2023Quote: ... Isolated RNA (0.5-1 µg) was then used for reverse transcription into first strand cDNA via a SensiFast cDNA synthesis kit (Bioline). RNA spike I (TATAA ...
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bioRxiv - Evolutionary Biology 2022Quote: qPCR was performed on each of the samples using an Applied Biosystems StepOnePlus system with a Sensifast Hi-Rox Sybr kit (Bioline). Cycle conditions were as follows ...
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bioRxiv - Evolutionary Biology 2022Quote: ... qRT-PCR was carried out on 1:2 diluted cDNA on an Applied Biosystems StepOnePlus system using a Sensifast Hi-Rox SYBR kit (Bioline). Cycle conditions were as follows ...
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bioRxiv - Evolutionary Biology 2023Quote: The relative quantity of the reverse transcribed target cDNA was assessed using magnetic induction cycler (micPCR, Bio Molecular Systems) and SensiFAST SYBR Lo-ROX Kit (Bioline). For each gene ...
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bioRxiv - Cell Biology 2023Quote: ... RNA was isolated from lung homogenates from mice exposed to E-cigarettes (with and without nicotine) and room air (SHAM) using Isolate II RNA Mini Kit (Bioline) and cDNA was synthesized using SensiFAST cDNA Synthesis kit (Bioline) ...
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bioRxiv - Cell Biology 2023Quote: ... 150 ng of total RNA was converted to complementary DNA (cDNA) using SensiFASTTM cDNA synthesis kit following manufacturer instructions (Bioline) and qPCR performed using SensiFAST™ SYBR® Lo-ROX Kit following manufacturer instructions (Bioline) ...
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bioRxiv - Microbiology 2023Quote: ... 0.2 ml chloroform was added for phase separation using phase lock gel heavy tubes and RNA isolation from the aqueous phase was obtained by using ISOLATE II RNA Mini Kit (Bioline). RNA quality was verified with Agilent 2100 Bioanalyzer System using RNA Nano Chips (Agilent Technologies) ...