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Citations for Bioline :
751 - 800 of 883 citations for Human Single stranded DNA binding protein 4 SSBP4 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... The qRT-PCR reaction was performed using 35 μL SensiFAST™ Probe LoROX One-Step Kit (Bioline, Taunton, MA) and 15 μL of extracted eluate ...
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bioRxiv - Neuroscience 2022Quote: ... RT-qPCR was performed with 1/10 diluted cDNA with the SensiFAST SYBR no-rox kit (Bioline, London, UK) and Lightcycler® 480 (Roche Molecular Biochemicals ...
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bioRxiv - Cancer Biology 2023Quote: ... 0.25uM forward and reverse oligo primers (see supplementary table 6) and SensiFAST SYBR Hi-ROX Kit (Bioline, BIO-92005) in 384-well plates with the LightCycler 480 Instrument II (Roche ...
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bioRxiv - Neuroscience 2023Quote: ... Real-time RT-PCR was performed using a SensiFastTM SYBR® Hi-ROX kit (#BIO-92020; Bioline USA Inc.) in an Applied Biosystems QuantStudio 7 Flex Real-Time PCR system (Thermo Fisher Scientific Inc.) ...
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bioRxiv - Microbiology 2023Quote: ... qRT-PCR analysis was performed in triplicate on 50 ng of cDNA using the SensiFAST SYBR & Fluorescein kit (Bioline) with a Roche Lightcycler 96 ...
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bioRxiv - Genomics 2023Quote: ... the k13 and pfcrt loci was amplified directly from recrudesced infected blood using the MyTaq Blood-PCR Kit (Bioline) (68) ...
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bioRxiv - Genetics 2023Quote: RNA was isolated from cells with Isolate II RNA Mini Kit according to the manufacturer’s instructions (Bioline, BIO-52702). 1 μg of RNA was converted to cDNA using qScript (Quanta ...
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bioRxiv - Biochemistry 2023Quote: ... 1μg of RNA was used in a 20-μl reaction mixture by using a SensiFast cDNA synthesis kit (Bioline). For real-time PCR analysis ...
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bioRxiv - Plant Biology 2023Quote: ... Real-time RT-PCR was performed on 1:10 diluted cDNA using the SensiFAST SYBR No-ROX Kit (Bioline). Three biological and technical replicates were analyzed per genotype ...
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bioRxiv - Developmental Biology 2024Quote: ... 0.25– 1 µg of RNA was reverse-transcribed into cDNA using the SensiFAST cDNA Synthesis Kit (Bioline, BIO-65054). Real-time PCR was performed on the CFX384 Touch real-time PCR detection system (Bio-Rad ...
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bioRxiv - Cell Biology 2020Quote: ... cDNA was used to analyse differences in gene expression by qRT-PCR employing SensiMix SYBR low-Rox KIT (Bioline, Germany) along with gene specific forward and reverse primers (250 nM ...
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bioRxiv - Cell Biology 2020Quote: ... The relative amount of PAK transcript variants was measured by quantitative real-time PCR (qPCR) using SensiFAST SYBR No-ROX Kit (Bioline) and calculated using Bio-Rad CFX Manager Software ...
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bioRxiv - Evolutionary Biology 2020Quote: ... For each gene a total of 8 clones from each plant were isolated from overnight LB cultures using an ISOLATE II Plasmid Mini Kit (Bioline) prior to sequencing with universal M13F and M13R primers by GATC Biotech (Konstanz ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Telomere length was measured using real-time quantitative PCR (qPCR) and a SensiMix SYBR No-ROX Kit (Bioline, Sydney, Australia). The telomere primers used were Tel1b (5’-CGGTTTGTTTGGGTTTGGGTTT GGGTTTGGGTTTGGGTT-3’ ...
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bioRxiv - Developmental Biology 2020Quote: ... one μg of each template was synthesized into cDNA with a standard reaction mix (SensiFAST™ cDNA Synthesis Kit, Bioline) in a thermal cycler (Bio-Rad T100TM Thermal Cycler ...
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bioRxiv - Genomics 2020Quote: ... Pluripotency and early differentiation marker as well as selected known imprinted genes expression was determined by qPCR using the Sensifast SYBR No Rox-Kit (Bioline).
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bioRxiv - Molecular Biology 2020Quote: ... For RT-qPCR cDNA was prepared from 1 µg of total RNA using the SensiFAST cDNA Synthesis Kit (BIO-65054, Bioline) and analysed by qPCR using the SensiFast SYBR Lo-ROX Kit (BIO-94050 ...
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bioRxiv - Molecular Biology 2021Quote: ... and lysozyme were determined with a Real Time Thermal cycler qTower (Analytical Jena) system using a SensiFast SYBR kit (Bioline) according to the supplier’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... cDNA products were used as the template for quantitative reverse transcription-PCR (qRT-PCR) using the SensiMix SYBR No-ROX kit (Bioline). For relative quantification of gene expression ...
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bioRxiv - Molecular Biology 2021Quote: ... a 10 μl qPCR reaction is set up using SensiFAST SYBR No-ROX kit (FroggaBio/Bioline, catalogue number BIO-98050), forward and reverse primers (Table 2) ...
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bioRxiv - Microbiology 2019Quote: ... Reverse transcription and quantitative real-time PCRs (qRT-PCR) were performed using the SensiFast SYBR No-ROX One Step kit (Bioline) in a Rotor-Gene Q Lightcycler (Qiagen) ...
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bioRxiv - Developmental Biology 2019Quote: ... Embryos were dechorionated and washed with 100% ethanol prior to RNA extraction using the ISOLATE II RNA Mini Kit’s protocol (Bioline, UK). The extracted RNA was quantified using Nanodrop (Thermo Fisher Scientific) ...
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bioRxiv - Evolutionary Biology 2021Quote: ... qRT-PCR was carried out on 1:10 diluted cDNA on an Applied Biosystems StepOnePlus system using Sensifast Hi-Rox Sybr kit (Bioline). Cycle conditions were as follows ...
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bioRxiv - Plant Biology 2020Quote: ... Quantitative real-time PCR reactions using cDNA samples as template were carried out using a SensiFAST SYBR No-ROX Kit (Bioline) in a CFX96 Touch (BIO-RAD ...
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bioRxiv - Microbiology 2020Quote: ... Influenza virus M gene copy number per μL RNA was determined by qRT-PCR using the SensiFAST Probe Lo-ROX One-Step qRT-PCR System Kit (Bioline) on the ABI 7500 Real Time PCR System (Applied Biosystems ...
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bioRxiv - Neuroscience 2020Quote: ... Quantitative PCR (qPCR) was carried out using the cDNA generated previously employing the SensiFAST SYBR mix No-Rox Kit (Bioline, UK in a Rotor-Gene 6000 real-time PCR cycler (Corbett Research ...
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bioRxiv - Microbiology 2021Quote: ... Quantitative real time-PCR (qRT-PCR) analysis was performed in triplicate on 50 ng cDNA using the SensiFAST SYBR & Fluorescein kit (Bioline) and a Roche Lightcycler 96 ...
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bioRxiv - Neuroscience 2020Quote: ... The cDNA products were diluted 10 times and used for qPCR using a StepOnePlusTM instrument (Applied Biosystem, USA) and SensiFAST SYBR Hi-ROX Kit (Bioline) following the manufacturer’s guidelines ...
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bioRxiv - Microbiology 2021Quote: ... Viral vRNA and mRNA were detected by polarity-specific quantitative RT-PCR using the SensiFast Probe No-ROX one-step kit (Bioline). Each 20 µL reaction contained 5 μl of RNA ...
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bioRxiv - Microbiology 2021Quote: RNA from GP barley infected with Fg-IFA65 at 5 dpi and an uninfected control was extracted with the Isolate II plant miRNA kit (Bioline). 1 µg of RNA (>200 nt ...
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bioRxiv - Microbiology 2021Quote: qRT-PCR was used to quantify transcript levels of specific quorum sensing-controlled genes in DS40M4 and was performed using the SensiFast SYBR Hi-ROX One-Step Kit (Bioline) according to the manufacturer’s guidelines ...
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bioRxiv - Microbiology 2021Quote: Real-time qRT-PCR (quantitative reverse transcription PCR) was used to quantify transcript levels using the SensiFast SYBR Hi-ROX One-Step Kit (Bioline) according to the manufacturer’s guidelines ...
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bioRxiv - Cancer Biology 2021Quote: ... and qPCR reactions were conducted using gene-specific primers with SensiFAST SYBR No-ROX kit (Bioline, #Cat no: BIO-98005) as described previously (Sibai et al. ...
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bioRxiv - Microbiology 2022Quote: ... Extracted RNA was subsequently assayed for a short fragment of the influenza A matrix gene (Spackman et al. 2002) using the SensiFAST Probe Lo-Rox qPCR Kit (Bioline). A cycle threshold (Ct ...
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bioRxiv - Developmental Biology 2021Quote: ... washed in water and 100% ethanol prior to RNA extraction using the ISOLATE II RNA Mini Kit’s protocol (Bioline, UK). The extracted RNA was quantified using Nanodrop (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2020Quote: ... Detection and quantification of viruses was conducted by qRT-PCR using the SensiFAST probe no-ROX one step kit (Bioline). The assay for detection of PVM uses primers and probe targeting the SH gene (Forward primer ...
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bioRxiv - Molecular Biology 2020Quote: ... qPCRs were run using 2 µl cDNA (diluted 1:1) in a 10 µl reaction with 5 µl SensiFAST SBYR No-ROX Kit (Bioline) and 0.1 µM of each primer ...
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bioRxiv - Genetics 2019Quote: ... Ear punches taken to identify animals during routine colony maintenance were placed directly into Buffer A from the MyTaq Extract-PCR kit (Bioline).
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bioRxiv - Molecular Biology 2020Quote: ... The purified PCR product was run on a 1% agarose gel cut out to remove remaining primer dimers and cleaned with PCR Isolate II PCR and Gel Kit (Bioline). The purified libraries were sequenced on an Illumina HiSeq2500 or MiSeq depending on the expected complexity of the library.
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bioRxiv - Microbiology 2019Quote: ... Samples were ground to a fine powder in liquid nitrogen and total RNA was isolated with the isolate II RNA Mini Kit (Bioline). After DNase treatment (Promega) ...
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bioRxiv - Neuroscience 2021Quote: ... The cDNA was then diluted and used as template for qPCR, which was performed with a StepOnePlus instrument (Applied Biosystem, USA) and SensiFAST SYBR Hi-ROX Kit (Bioline) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... The real-time quantitative PCR (RT-qPCR) analysis was carried out by using the 2x SensiMix PLUS SYBR No-ROX Kit (Bioline) in a Rotor-Gene 6000 (Corbett ...
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bioRxiv - Cell Biology 2020Quote: Real-Time PCR analysis was performed using TaqMan gene expression assays using primers (see Resources Table) and SensiFast Probe Lo-ROX Kit (cat.# BIO-84020, Bioline) following manufacturer’s recommendation ...
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bioRxiv - Cell Biology 2021Quote: mRNA was isolated using NucleoSpin® RNA Plus (Machery-Nagel) and 1 µg of total mRNA was reverse-transcribed into cDNA using SensiFASTTM cDNA Synthesis Kit (Bioline) according to the manufacture’s protocol ...
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bioRxiv - Biochemistry 2022Quote: ... First-strand cDNA synthesis and subsequent real-time PCR was performed using SensiFast ™ SYBR No-Rox One-Step Kit (Cat.No. BIO- 72005 (Bioline)) ...
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bioRxiv - Genetics 2022Quote: ... 1 μg of RNA was then reverse transcribed with random hexamer and anchored oligo dT primers using SensiFast cDNA synthesis kit (Bioline). Quantitative PCR was performed on the diluted cDNA using SensiFast probe (Bioline ...
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bioRxiv - Genetics 2022Quote: The frequency of proximity ligated fragments containing the Myc promoter and other downstream regions was then quantified on the BioRad C1000 Thermo cycler using the SensiMix SYBR NOROX kit (Bioline) with 60°C annealing temperature ...
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bioRxiv - Molecular Biology 2022Quote: MtDNA copy number was quantified by quantitative PCR (qPCR) using the SensiFAST™ SYBR No-ROX Kit (BIO-98020, Bioline), by amplification of three mtDNA fragments (12S-rRNA ...
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bioRxiv - Microbiology 2023Quote: ... Reverse transcription and quantitative real-time PCRs (qRT-PCR) were performed using the SensiFast SYBR No-ROX One Step kit (Bioline) in a Rotor-Gene Q lightcycler (Qiagen) ...
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bioRxiv - Cell Biology 2023Quote: ... and used as a template for quantitative polymerase chain reaction with reverse transcription (qRT–PCR) analysis using SensiFAST SYBR Lo-ROX Kit (Bioline). Samples were analyzed using a QuantStudio 3 Real-Time PCR System (ThermoFisher) ...