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Citations for Bioline :
651 - 700 of 744 citations for Human Gap Junction Alpha 8 Protein CX50 GJA8 ELISA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Evolutionary Biology 2023Quote: The relative quantity of the reverse transcribed target cDNA was assessed using magnetic induction cycler (micPCR, Bio Molecular Systems) and SensiFAST SYBR Lo-ROX Kit (Bioline). For each gene ...
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bioRxiv - Cell Biology 2023Quote: ... RNA was isolated from lung homogenates from mice exposed to E-cigarettes (with and without nicotine) and room air (SHAM) using Isolate II RNA Mini Kit (Bioline) and cDNA was synthesized using SensiFAST cDNA Synthesis kit (Bioline) ...
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bioRxiv - Cell Biology 2023Quote: ... 150 ng of total RNA was converted to complementary DNA (cDNA) using SensiFASTTM cDNA synthesis kit following manufacturer instructions (Bioline) and qPCR performed using SensiFAST™ SYBR® Lo-ROX Kit following manufacturer instructions (Bioline) ...
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bioRxiv - Microbiology 2023Quote: ... 0.2 ml chloroform was added for phase separation using phase lock gel heavy tubes and RNA isolation from the aqueous phase was obtained by using ISOLATE II RNA Mini Kit (Bioline). RNA quality was verified with Agilent 2100 Bioanalyzer System using RNA Nano Chips (Agilent Technologies) ...
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bioRxiv - Microbiology 2023Quote: ... 0.2 ml chloroform was added for phase separation using phase lock gel heavy tubes and RNA isolation from the aqueous phase was obtained by using ISOLATE II RNA Mini Kit (Bioline). RNA quality was verified with Agilent 2100 Bioanalyzer System using RNA Nano Chips (Agilent Technologies) ...
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bioRxiv - Plant Biology 2023Quote: ... and the 200 to 400 bp range was purified by gel extraction using Isolate II PCR and Gel Kit (Bioline). Libraries were sequenced (100 bp paired-end ...
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bioRxiv - Microbiology 2023Quote: ... If a band was visualised at ∼480 bp the PCR product was purified using an ISOLATE II PCR and Gel Kit (Bioline).
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bioRxiv - Microbiology 2023Quote: ... PCR products that produced bands of the correct size were purified using the ISOLATE II PCR and Gel Kit (Bioline) as per manufacturer’s protocol and submitted for sequencing using an Applied Biosystems 3730xl capillary analyser (Macrogen) ...
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bioRxiv - Microbiology 2023Quote: ... was performed in technical triplicate with 50 ng of cDNA per reaction using the SensiFAST SYBR & Fluorescein Kit from Bioline, on a Roche Lightcycler 96 ...
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bioRxiv - Microbiology 2024Quote: ... The PCR products were run on 1% agarose gel and the corresponding band was gel extracted using PCR gel extraction kit (Bioline) and further sequenced by Sanger method using one of the PCR primers ...
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bioRxiv - Microbiology 2024Quote: ... The PCR products were run on 1% agarose gel and the corresponding band was gel extracted using PCR gel extraction kit (Bioline) and further sequenced by Sanger method using one of the PCR primers ...
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bioRxiv - Neuroscience 2024Quote: ... or 1 μg (4 weeks of treatment) of the total RNA was reverse-transcribed into cDNA using a Tetro cDNA synthesis kit (BIO-65042, Bioline). Quantitative PCR (RT-qPCR ...
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bioRxiv - Microbiology 2024Quote: ... was used to quantify transcript levels of T3SS genes in different regulatory conditions and was performed using the SensiFast SYBR Hi-ROX One-Step Kit (Bioline) according to the manufacturer’s guidelines ...
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bioRxiv - Neuroscience 2024Quote: ... Samples were DNase treated and subsequent reverse transcription to obtain cDNA was performed using the SensiFAST cDNA synthesis kit (BioLine). cDNA samples were then used as templates for real-time PCR assays performed on a Bio-Rad CFX system using the SensiFAST SYBR No-ROX Kit (BioLine) ...
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bioRxiv - Neuroscience 2024Quote: ... cDNA samples were then used as templates for real-time PCR assays performed on a Bio-Rad CFX system using the SensiFAST SYBR No-ROX Kit (BioLine). Alpha-tubulin mRNA was used as a reference gene for normalization ...
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bioRxiv - Neuroscience 2024Quote: ... cDNA samples were then used as templates for real-time PCR assays performed on a Bio-Rad CFX system using the SensiFAST SYBR No-ROX Kit (BioLine). Beta-actin mRNA was used as reference gene for normalization ...
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bioRxiv - Genomics 2024Quote: ... 1 µg of the entry vector was also digested with EcoRI-HF and NheI-HF and the linearized product was purified from a 2% agarose gel using PCR Isolate II PCR and Gel Kit (Bioline). The digested and purified reporter pool was then ligated into 80 ng of the linearized entry vector using Takara ligation kit v1.0 (#6021 ...
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bioRxiv - Immunology 2024Quote: ... Quantitative PCR (qPCR) was performed using a SensiFAST™ SYBR® No-ROX Kit (Roche Bioline, cat. no. BIO-98020). Expression levels of all genes were normalized to the geometric mean of housekeeping genes ...
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bioRxiv - Evolutionary Biology 2024Quote: q(RT)-PCR was performed for each virus and the housekeeping gene RPL32 using the Sensifast Lo-Rox SYBR kit (Bioline) on an Applied Biosystems QuantStudio 3 (for primers and cycling conditions ...
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bioRxiv - Cell Biology 2020Quote: ... PCR bands were extracted from the agarose gel and purified using and Isolate II PCR and gel kit (Bioline, London, UK) and sent for Sanger sequencing (GATC Biotech ...
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bioRxiv - Microbiology 2021Quote: ... Each reaction (20 μL) consisted of 1 μL of cDNA substrate and 19 μL of a SensiFAST No-ROX Kit Master Mix (Bioline, UK). Following primer optimisation ...
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bioRxiv - Developmental Biology 2020Quote: cDNA was diluted 1:10 in nuclease-free water and subsequently applied for qRT-PCR using the SensiFast™SYBR HiROX Kit (Bioline) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2020Quote: ... Quantitative real-time RT–PCR (qRT–PCR) was carried out using the SensiFast™SYBR Hi-ROX One-Step Kit (Bioline) as described (Wobbe et al. ...
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bioRxiv - Genomics 2020Quote: ... total RNA of both uninfected and DENV1-infected samples were subjected cDNA synthesis using Tetro cDNA synthesis kit (Bioline, BIO-65042). Reverse oligo of DENV1 specific primer was used in cDNA synthesis ...
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bioRxiv - Neuroscience 2020Quote: ... Reverse transcription of 500 ng of motor cortex RNA was performed using the Tetro cDNA Synthesis kit (Bioline, Meridian Bioscience, USA) with random hexamer primers according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: ... RT-PCR reactions were set up in a 384-well format using 2X SensiFAST Probe No-ROX Kit (Bioline, BIO-86005) and 1 µl of cDNA per reaction in a total volume of 10 µl ...
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bioRxiv - Microbiology 2022Quote: ... A few microliters of each PCR product were run on an agarose gel to assess the success of the PCR reaction and the remains cleaned through an Isolate II PCR and Gel kit (Bioline, USA) and sent for sequencing with primer C1-N-2191 ...
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bioRxiv - Microbiology 2021Quote: ... the K13 locus was amplified directly from whole blood using the primer pair p26+p27 (Table S9) and the MyTaq™ Blood-PCR Kit (Bioline). Primer pairs p39+p40 and p50+p51 were used to amplify fd and mdr2 ...
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bioRxiv - Immunology 2022Quote: ... genomic DNA (gDNA) was isolated from mouse brains or dural meninges using the Isolate II Genomic DNA Kit (Bioline, BIO-52067) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... RT-qPCRs were carried out using the SensiFAST SYBR No-ROX kit according to the manufacturer’s instructions (Bioline, London, United Kingdom) on a CFX96™ real-time instrument (Bio-Rad) ...
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bioRxiv - Pathology 2020Quote: Wheat curl mite DNA was extracted from single mites using the MyTaq™ Extract-PCR Kit (Bioline Meridian Bioscience, London, UK)) according to the manufacturer’s recommendations with the exception of the amount of starting material being less than 3 mg ...
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bioRxiv - Molecular Biology 2020Quote: ... the cells were collected and genomic DNA was extracted using the ISOLATE II Genomic DNA kit (Bioline cat. no. BIO-52067). PCR was performed in two steps and pooled experiments were performed in triplicates for a higher coverage ...
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bioRxiv - Microbiology 2020Quote: Bacterial cultures were grown in Luria Bertani (LB) broth for 18-24h at 37 °C and DNA was extracted using the Isolate II Genomic DNA Kit (Bioline, UK) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... Primers were designed using NCBI primer blast (Table 3) and qRT-PCR was performed using SensiFAST™ SYBR Lo-ROX Kit (#BIO-94020, Bioline) on the BIO-RAD CFX38 Real time PCR machine ...
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bioRxiv - Physiology 2021Quote: ... the K13 locus was amplified directly from whole blood using the primer pair p26+p27 (Supplementary file 5) and the MyTaq™ Blood-PCR Kit (Bioline). Primer pairs p39+p40 and p50+p51 were used to amplify fd and mdr2 ...
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bioRxiv - Immunology 2021Quote: ... The master mix was prepared with 2.5 mL of 2X buffer containing Taq-polymerase was prepared from the TaqMan RT-PCR kit (Bioline #BIO-78005). From the kit ...
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bioRxiv - Microbiology 2021Quote: ... The number of mycobacterial cells in the sample was quantified using the SensiFAST SYBR No-ROX qPCR kit (Bioline, London, UK) on bacterial DNA according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... cDNA was then synthesized from 2.5 μg RNA in a 20 μL reaction using both random hexamers and oligo(dT) primers provided with the Tetro cDNA Synthesis kit (Bioline™). Ten defense genes were quantified using the SYBR Green qRT-PCR kit on a ViiA™ 7 sequence detection system ...
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bioRxiv - Genetics 2024Quote: RT-qPCR was performed (in triplicate) using the Bio-Rad cycler CFX 96 and the SensiFAST SYBR Green Kit (Catalog Number BIO-98005, BIOLINE, USA). Gene expression levels were normalized to housekeeping genes pmp-3 ...
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bioRxiv - Plant Biology 2023Quote: ... The diluted cDNA was then used as a template for quantitative Real-Time PCR following the manufacturer’s instructions (SensiFAST™ SYBR® No-ROX Kit; Bioline). Samples were amplified following the manufacturer’s instructions and fluorescence was monitored with a CFX384 Touch™ Real-Time PCR Detection System (Bio-Rad Laboratories ...
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bioRxiv - Immunology 2023Quote: ... real time q-PCR was performed using the SensiFAST™ SYBR® Kit according to the manufacturer’s instruction (BIO-94050, Bioline) and two pairs of primers (NSP4/eGFP forward primer ...
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bioRxiv - Immunology 2023Quote: ... The total qPCR reaction volume was 1.5 μl and consisted of 0.5 μl of cDNA (dilution 1/12) and 1 μl of SensiFAST™ SYBR® No-ROX Kit (Bioline) containing 0.4 μM of PCR primer (Eurogenetec SA)(primer list in sup ...
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bioRxiv - Microbiology 2024Quote: ... A 1369F/1492R amplicon was generated from Aliivibrio finisterrensis LMG 23869 of 151 bp size using the MyTaq Direct PCR kit (Bioline Australia) and utilizing a PTC-200 Thermal Cycler (MJ Research) ...
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bioRxiv - Microbiology 2024Quote: ... 5’-[FAM]-CACAGGAGC-[ZEN]-CAAGAGTGAAGAACAGT-[3IABkFQ]-3’) was used for quantification using the SensiFAST™Probe Lo-ROX One-Step Kit (Bioline). 0.2 μL of 10 μM probes and 0.8 μL of 20 μM primers were used for both targets ...
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bioRxiv - Plant Biology 2024Quote: ... The total RNA was then converted to cDNA by reverse transcription with the SensiFAST™ cDNA Synthesis Kit (Bioline, https://www.bioline.com). For each quantitative real-time RT-PCR reaction ...
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bioRxiv - Developmental Biology 2024Quote: ... S3) along with 1.5 µl of PCR water and 5 µl SensiFAST™ SYBR® No-ROX Kit (BIO-98020, Bioline) or with TaqMan probes (Table S4) ...
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bioRxiv - Microbiology 2020Quote: ... 250-1,000 ng of extracted RNA was converted to cDNA in a reaction volume of 20 μl using the Tetro cDNA synthesis kit (BIO-65043, Bioline, London, UK). The cDNA was then amplified using an SYBR green RT-PCR kit (06924204001 ...
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bioRxiv - Developmental Biology 2020Quote: ... and mClover3-bElys constructs were cloned from bovine fibroblast or bovine oocyte cDNA libraries made using a SensiFAST cDNA synthesis kit (Bioline, BIO-65053). The primers (KASH5-DN cloning ...
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bioRxiv - Cancer Biology 2022Quote: ... mRNA expression was determined with a quantitative real time PCR SYBR Green SensiFAST™ SYBR® No-ROX Kit (Bioline, London, UK) on a Light Cycler® 480 Instrument II (Roche Life Sciences) ...
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bioRxiv - Molecular Biology 2020Quote: Complementary DNA (cDNA) used for in situ hybridisation and quantitative PCR (qPCR) was synthesised using the Tetro cDNA synthesis kit (Bioline, London, UK) with oligo-dT primers according to the manufacturers’ instructions.