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Citations for Bioline :
601 - 650 of 857 citations for Rat EGF Latrophilin And Seven Transmembrane Domain Containing Protein 1 ELTD1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... and on-column DNase treatment and purification were performed using the ISOLATE II RNA mini kit (Bioline) or RNeasy Mini kit (Qiagen ...
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Quantitative Comparison of Presenilin Protein Expression Reveals Greater Activity of PS2-γ-SecretasebioRxiv - Cell Biology 2023Quote: ... 0.5 µg of RNA was used to generate cDNA using Tetro cDNA synthesis kit (Bioline BIO-65043) in a 10 µl reaction using a 1:1 ratio of random hexamer and oligo (dT)18 primer mix as per manufacturer instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... and 500 ng RNA per sample was used as input to the SensiFAST cDNA Synthesis Kit (Bioline), including -RT controls ...
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bioRxiv - Microbiology 2024Quote: ... qRT-PCR analysis was performed on 50 ng of cDNA using the SensiFAST SYBR & Fluorescein kit (Bioline) with a Roche Lightcycler 96 ...
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bioRxiv - Cancer Biology 2024Quote: Quantitative RT-PCR analyses were performed in technical triplicates with SensiMix SYBR kit (Bioline, Cat# QT605–20) using the ViiA™ 7 Real Time PCR System (Life Technologies) ...
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bioRxiv - Microbiology 2021Quote: ... using 5 μl product with 1 μl 5X loading dye (BioLine) to verify bands of approximately 450 bp ...
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bioRxiv - Synthetic Biology 2023Quote: ... Molecular weight markers used were the 1 kb HyperLadder (from Bioline). T4 DNA ligase and buffer were purchased from New England Biolabs ...
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bioRxiv - Molecular Biology 2023Quote: ... The resultant bands were compared to a 1 Kb HyperLadder (Bioline) using the expected sizes per gene region as follows ...
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bioRxiv - Cell Biology 2020Quote: Total RNA was extracted from treated cells via an ISOLATE II RNA mini kit (Bioline, Cat: BIO-52073) following manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: Plasmodium screening of human subjects was done in the regions surrounding Mbita using RDT kits (SD Bioline, UK). Microscopy was carried out on RDT-positive samples to confirm the presence of P ...
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bioRxiv - Cell Biology 2022Quote: ... Purified RNA (500 ng) was transcribed into cDNA with Tetro cDNA Synthesis Kit (Bioline Reagents Limited, London, UK) to analyze genes expression ...
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bioRxiv - Cancer Biology 2022Quote: ... The purified DNAs were amplified with SYBR Green SensiFAST™ SYBR® No-ROX Kit (Bioline, London, UK) by real-time qPCR.
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bioRxiv - Molecular Biology 2021Quote: ... qPCR quantification of DNA samples was carried out using the SensiFAST SYBR Hi-ROX Kit (Bioline, BIO-92005) in 96-well plates using a ABI PRISM 7000 system (Applied Biosystems) ...
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bioRxiv - Microbiology 2020Quote: ... PCR products were purified using the Bioline Isolate II PCR and Gel kit (Bioline, Cat. No. BIO-52060). Samples were sequenced using the Illumina Miseq platform ...
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bioRxiv - Molecular Biology 2019Quote: ... Real-time PCRs were analyzed by SensiFAST™ SYBR® Hi-ROX Kit (BIO-92020, Bioline, London, UK). A PCR program was used as described ...
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bioRxiv - Plant Biology 2020Quote: ... RNA was used for reverse transcription according to the protocol of the SensiFASTtm cDNA synthesis kit from Bioline. PCR reactions were carried out with 5 μL of 100x diluted cDNA solution ...
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bioRxiv - Microbiology 2020Quote: ... cDNA was synthesised using SuperScript III reverse transcriptase from RNA extracted using ISOLATE II RNA Mini Kit (Bioline) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... Bands of correct size were cut out and purified using the Isolate II PCR and gel kit (Bioline). 50 ng of vector and 150 ng of each fragment were mixed with 3 μl of HiFi DNA Master Mix (NEB ...
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bioRxiv - Cancer Biology 2021Quote: ... The conversion of messenger RNA into cDNA was performed using the Sensifast cDNA synthesis kit (Bioline, BIO- 65053). cDNA was then amplified by PCR using specific primers for each gene designed with Primer-blast software (https://www.ncbi.nlm.nih.gov/tools/primer-blast/ ...
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bioRxiv - Plant Biology 2021Quote: ... Reverse transcription was performed in 20mL with 1mg of total RNA using the Tetro cDNA synthesis kit (Bioline). RT-negative (no enzyme ...
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bioRxiv - Microbiology 2021Quote: ... Two micrograms of total RNA was reverse-transcribed with Sensi-FAST™ cDNA Synthesis Kit (Bioline, Memphis, USA). The amounts of p16 ...
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bioRxiv - Molecular Biology 2019Quote: DNA was extracted from surface-sterilized whole insects using the ISOLATE II Genomic DNA Kit (Bioline, London, UK) according to the manufacturer’s instructions ...
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bioRxiv - Pathology 2019Quote: ... PCR amplicons of each sample were pulled together and purified using Isolate II PCR and gel Kit (BIOLINE) and about 100 ng of DNA was used in library preparation ...
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bioRxiv - Evolutionary Biology 2020Quote: ... DNA from historical museum toepad samples was extracted using the Bioline Isolate II Genomic DNA extraction kit (Bioline), but suspending tissues in 400 μL lysis buffer with 25 μL proteinase k and incubated overnight at 55 °C ...
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bioRxiv - Microbiology 2021Quote: ... Amplicons of appropriate size were excised and purified using an Isolate II PCR and Gel kit (Bioline, UK). The purified amplicons were included in cycle sequence reactions ...
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bioRxiv - Immunology 2021Quote: ... Real time quantitative PCR (RT-qPCR) analysis was carried out using the SensiMix SYBR No-Rox kit (Bioline), using primers and conditions shown in Supplemental Table 1 ...
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bioRxiv - Microbiology 2022Quote: Faecal DNA was extracted from FecalSwabTM media (200µL) using the ISOLATE II Fecal DNA Kit (Bioline, Sydney, Australia) following the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2022Quote: Isolation of total RNA and cDNA synthesis was performed by using the Isolate II RNA Mini Kit (Bioline) and SensiFAST cDNA Synthesis Kit (Bioline) ...
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bioRxiv - Cell Biology 2022Quote: ... Real-time PCR analysis was performed according to the manufacturer’s protocol (SensiMix™ SYBR® & Fluorescein Kit, Bioline) with a MyiQ Single-Color Real-Time PCR detection system (Bio-Rad ...
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bioRxiv - Genetics 2022Quote: The relative amount of PCR product was quantified by qPCR using SensiFASTTM SYBR® Hi -ROX Kit (Bioline). The sequences of primers used for the quantitative detection of the chromosomal loci are listed in Suppl Table ST6 ...
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bioRxiv - Microbiology 2022Quote: ... conventional PCR assays were replaced with qPCR assays with SensiFAST™ SYBR® No-ROX Kit (Bioline, Australia). The cycling conditions included an initial denaturation at 95°C for 3 min followed by 28 -40 cycles of 95°C for 5 sec ...
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bioRxiv - Plant Biology 2023Quote: ... The total RNA was extracted from ground root samples using an ISOLATE II Plant Mini Kit (Bioline, Australia), cDNA was synthesised from 1ug of RNA using a SensiFAST cDNA Synthesis Kit (Bioline ...
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bioRxiv - Cancer Biology 2022Quote: ... transcripts were amplified directly on RNA samples using a one-step qRT-PCR kit with SYBR green (Bioline). Primers used against DDIT3 (CHOP ...
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bioRxiv - Plant Biology 2024Quote: ... This template was then used to quantify relative mRNA abundance using the SensiMix SYBR Low-ROX kit (Bioline), a LightCycler® 480 (Roche ...
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bioRxiv - Neuroscience 2024Quote: ... and qPCR performed in technical triplicate using SensiFAST™ SYBR® Lo-ROX Kit following manufacturer instructions (Bioline) on QuantStudioTM 5 Real-Time PCR system as previously described (34,35,37,41) ...
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bioRxiv - Cancer Biology 2022Quote: ... cDNA was prepared for RT-qPCR using the SensiFAST cDNA preparation kit according to manufacturer instructions (Bioline #65054). 1μL of cDNA was used per RT-qPCR reaction prepared with SYBR Lo-ROX (Bioline #94020) ...
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bioRxiv - Plant Biology 2023Quote: Measurements were carried out in 96-well plates using the SensiFAST™ SYBR® Kit (Bioline, Luckenwalde, Germany) in a LightCycler® 480 (Roche) ...
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bioRxiv - Evolutionary Biology 2023Quote: ... primers at a final concentration of 800nM and 6μL of SensiFAST™ SYBR® Lo-ROX Kit (Bioline). qPCR conditions were ...
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bioRxiv - Evolutionary Biology 2023Quote: ... 600 ng of isolated RNA was reverse transcribed to complementary DNA (cDNA) with SensiFAST cDNA Synthesis -kit (Bioline) following the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... cDNA was prepared for RT-qPCR using the SensiFAST cDNA preparation kit according to manufacturer instructions (Bioline #65054). 1µL of cDNA was used per RT-qPCR reaction prepared with SYBR Lo-ROX (Bioline #94020) ...
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Mapping chromatin state and transcriptional response in CIC-DUX4 undifferentiated round cell sarcomabioRxiv - Cancer Biology 2023Quote: ... Total RNA (1000 ng) was used in a reverse transcriptase reaction with the SensiFAST cDNA Synthesis Kit (Bioline). Quantitative PCR included 3 replicates per cDNA sample ...
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bioRxiv - Cell Biology 2024Quote: ... 1μg of total RNA was used to synthesize cDNA using the SensiFAST cDNA Synthesis Kit (Bioline, BIO-65054) following manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... Correct sized amplicons were then purified using an Isolate II PCR and Gel Kit (Bioline, Meridian Biosciences, Australia) following the manufacturer’s instructions for PCR clean-up and sent for Sanger sequencing at the Victorian Clinical Genetics Services (VCGS ...
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bioRxiv - Molecular Biology 2024Quote: ... Genomic DNA was extracted from parasite lines using an Isolate II Genomic DNA Kit (Bioline, Meridian Bioscience Australia), following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... buffer with 1 mg/ml Proteinase K (Bioline, Cat. No. BIO-37084) to pre-lyse the cells ...
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bioRxiv - Microbiology 2019Quote: ... A 1 kilobase molecular-weight size marker (Hyperladder 1KB from Bioline UK) was used to confirm that the 16S ribosomal RNA hypervariable region had been isolated (supplementary material) ...
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bioRxiv - Microbiology 2021Quote: ... 1% SDS (Sigma-Aldrich)] supplemented with 5 μL Proteinase K (20 mg mL−1; Bioline Australia) to lyse cells ...
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bioRxiv - Molecular Biology 2022Quote: ... supplemented with 1 mg/ml proteinase K (Bioline, cat. no. BIO-37084) by incubating them at 55 °C for at least 2 hours up to overnight ...
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bioRxiv - Genetics 2024Quote: ... Hyperladder 50 bp and 1 kb (both from Bioline, Tennessee, United States) were used as DNA fragment size markers.
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bioRxiv - Microbiology 2020Quote: ... cells were collected by centrifugation at 4000 rpm for 15 minutes and pellets used for RNA extraction by RNeasy kit according to the manufacturer’s protocol (Bioline). cDNA synthesis and RT-qPCR was performed as previously described (60) ...