Labshake search
Citations for Bioline :
601 - 650 of 797 citations for RNA Amplification kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2019Quote: ... Quantitative RT-PCR reactions were carried out using SYBR Green (SensiMix SYBR Low-ROX Kit) (BIOLINE) on 7500 MicroAmp Fast Optical 96-Well Reaction Plate (Applied Biosystems) ...
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bioRxiv - Cancer Biology 2021Quote: ... Quantitative RT-PCR was performed using SensiMix SYBR Low-ROX Kit (Bioline; annealing temperature – 60°C) in a Lightcycler 480 384-well plate (Roche) ...
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bioRxiv - Neuroscience 2021Quote: ... Conversion to cDNA was carried out using a SensiFAST™ cDNA synthesis kit (Bioline, London, UK). For qRT-PCR ...
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bioRxiv - Molecular Biology 2021Quote: ... Genomic DNA was isolated three days after transduction (ISOLATE II Genomic DNA kit, Bioline BIO-52067), without stabilization of the Dam fusions to keep expression at low levels ...
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bioRxiv - Microbiology 2020Quote: Genomic DNA was isolated using 200 μL of parasite culture (Isolate II Genomic DNA kit, Bioline). Candidate actinonin resistance genes were amplified using the primers listed in Supplementary Table 3 ...
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bioRxiv - Microbiology 2021Quote: ... qPCR reactions of all DNA samples were performed using the SensiFAST SYBR No-ROX Kit (Bioline) on a Mic (Magnetic Induction Cycler ...
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bioRxiv - Microbiology 2021Quote: qRT-PCR was performed using the SensiFAST Probe Lo-ROX One-Step kit (Bioline Meridian, Australia) with a reaction volume of 20µl ...
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bioRxiv - Cell Biology 2021Quote: ... The cDNA (100 ng) was analysed by quantitative PCR using SensiFASTTM SYBR Lo-ROX Kit (Bioline) in the 7500 Fast Real-Time PCR System (Applied Biosystems) ...
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bioRxiv - Neuroscience 2020Quote: ... after which cDNA was synthesized using the SensiFAST(tm) cDNA Synthesis Kit (Bioline, Taunton, MA, USA).
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bioRxiv - Evolutionary Biology 2022Quote: ... qPCR was performed on an Applied Biosystems StepOnePlus system using Sensifast Hi-Rox Sybr kit (Bioline) with the following PCR cycle ...
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bioRxiv - Genomics 2022Quote: ... frozen samples were harvested and extracted using the ISOLATE II Plant miRNA kit (Bioline, Sydney, Australia) as per the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... and was reverse transcribed to cDNA using the SensiFastTM cDNA synthesis kit (Bioline, GmbH, Luckenwalde, Germany). The primers for qRT-PCR were designed using the NCBI tool Primer-BLAST–NCBI–NIH software ...
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bioRxiv - Molecular Biology 2023Quote: ... and diluted 1:10 before quantification using the SensiFAST SYBR No-Rox kit (Bioline, BIO-98005). qRT-PCR was performed with 1 μM of each primer (a common reverse primer for both Trm1 isoforms and isoform-specific forward primers ...
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bioRxiv - Genetics 2023Quote: ... The RT-qPCR reactions were set up using SensiFAST SYBR No-ROX kit (Bioline, BIO-98005) in a Light Cycler 480 II system (Roche) ...
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bioRxiv - Genomics 2023Quote: Genomic DNA was extracted using either the Bioline Isolate II Genomic DNA Kit (Bioline, Eveleigh, Australia) following the manufacturer’s protocols ...
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bioRxiv - Biochemistry 2023Quote: ... using TaqMan™ Gene Expression Assays (FAM-labelled) and the SensiFASTTM Probe Hi-ROX kit (Bioline). The following TaqMan™ Gene Expression Assays were used ...
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bioRxiv - Plant Biology 2020Quote: ... the SensiFAST SYBR No-ROX Kit was used according to manufacturer’s recommendations (Bioline Reagents Ltd., London, UK). Primers used for semi quantitative RT-PCR can be seen in Table 2.
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bioRxiv - Neuroscience 2020Quote: ... 5 µl of (2x) SYBR green master mix (SensiFAST SYBR kit, Bioline, London, United Kingdom; BIO- 94005) was used and combined with 0.4 µl of a combined forward and reverse primer mix (10 uM of forward and reverse primers in the combined master mix ...
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bioRxiv - Evolutionary Biology 2021Quote: ... qRT-PCR was performed on an Applied Biosystems StepOnePlus system using Sensifast Hi-Rox Sybr kit (Bioline) with the following PCR cycle ...
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bioRxiv - Systems Biology 2020Quote: ... Naïve and primed marker expression was determined by qPCR using the Sensifast SYBR No Rox-Kit (Bioline). Primers are listed in Supplementary Table 6.
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bioRxiv - Plant Biology 2020Quote: ... Quantitative Real-Time PCR was performed using a SensiFAST™ SYBR® No-ROX Kit (Bioline; www.bioline.com). Fluorescence was monitored with a CFX384 thermal cycler (Bio-Rad) ...
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bioRxiv - Microbiology 2020Quote: ... Real-time RT PCR was performed using the SensiFAST™ Probe Lo-ROX one-step kit (Bioline). In each reaction the primers final concentration was 600nM and the probe concentration was 300nM ...
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bioRxiv - Plant Biology 2021Quote: ... qRT-PCR of protonema samples was conducted using the SensiFastTM SYBR No-ROX Kit (Bioline, Luckenwalde, Germany) in a LightCycler 480 (Roche ...
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bioRxiv - Microbiology 2020Quote: ... Real-time RT-PCR was conducted with SensiFAST™ Probe Lo-ROX One-Step Kit (Bioline, 78005) and analyzed with the 7500 Real Time PCR System (Applied Biosystems) ...
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bioRxiv - Genetics 2021Quote: Quantitative polymerase chain reaction (qPCR) was performed using SensiFAST™ SYBR No-ROX Kit (Bioline, BIO-98020) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: ... qPCR was performed with 500ng of cDNA and the SensiFast™ SYBR® No-ROX kit (Bioline) and a Lightcycler® 480/1536 (Roche ...
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bioRxiv - Immunology 2020Quote: ... (Aurogene, Rome, Italy) according to the manifacturer’s instructions and reverse transcripted to cDNA through SuperMix kit (Bioline). qPCR was carried out on a Rotor-Gene 6000 (Corbett ...
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bioRxiv - Cell Biology 2021Quote: ... RT-qPCR was performed using SensiFAST™ SYBR® Hi-ROX Kit (Bioline, cat. no. BIO-92020) in a 96-well white plate (Roche ...
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bioRxiv - Developmental Biology 2020Quote: ... All testing samples were used in qPCR according to SensiFASTTM SYBR® No-ROX Kit manual (Bioline). NvDsx qPCR primers were designed on the common region (exon 2-5 ...
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bioRxiv - Immunology 2021Quote: ... RT-PCR was carried out using the Bioline SensiFASTTM Probe Lo-ROX Kit (Bioline, Catalogue no.-84005) and Stratagene Mx3005P (Agilent Technologies) ...
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bioRxiv - Evolutionary Biology 2022Quote: ... qRT–PCR was performed on an Applied Biosystems StepOnePlus system using Sensifast Hi-Rox Sybr kit (Bioline) with 1 µl of complementary DNA (cDNA ...
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bioRxiv - Plant Biology 2022Quote: ... the SensiFAST SYBR No-ROX Kit was used according to manufacturer’s recommendations (Bioline Reagents Ltd., London, UK).
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bioRxiv - Microbiology 2022Quote: ... RT-PCR reaction was setup using SensiFast Probe No-ROX One- Step Kit (Bioline, Cat. BIO-76005) using the following primers/probes ...
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bioRxiv - Molecular Biology 2022Quote: ... Real- time PCR was performed using 2x SensiMix SYBR and Fluorescein Kit (Bioline, QT615-05, Luckenwalde, Germany), 20ng cDNA and pre-tested gene-specific primer sets (listed in Supplementary table 3) ...
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bioRxiv - Evolutionary Biology 2023Quote: ... qRT-PCR was carried out on 1:10 diluted cDNA using Sensifast Hi-Rox Sybr kit (Bioline). Two qRT-PCR reactions (technical replicates ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Quantitative analysis of the ERBB2 was performed using a SensiFAST™ SYBR Hi-ROX kit (Bioline, Canada). PCR amplification condition was applied as described previously [29] ...
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bioRxiv - Microbiology 2019Quote: Plasmodium screening of human subjects was done in the regions surrounding Mbita using RDT kits (SD Bioline, UK). Microscopy was carried out on RDT-positive samples to confirm the presence of P ...
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bioRxiv - Cancer Biology 2022Quote: ... The purified DNAs were amplified with SYBR Green SensiFAST™ SYBR® No-ROX Kit (Bioline, London, UK) by real-time qPCR.
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bioRxiv - Molecular Biology 2021Quote: ... qPCR quantification of DNA samples was carried out using the SensiFAST SYBR Hi-ROX Kit (Bioline, BIO-92005) in 96-well plates using a ABI PRISM 7000 system (Applied Biosystems) ...
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bioRxiv - Microbiology 2020Quote: ... PCR products were purified using the Bioline Isolate II PCR and Gel kit (Bioline, Cat. No. BIO-52060). Samples were sequenced using the Illumina Miseq platform ...
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bioRxiv - Molecular Biology 2019Quote: ... Real-time PCRs were analyzed by SensiFAST™ SYBR® Hi-ROX Kit (BIO-92020, Bioline, London, UK). A PCR program was used as described ...
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bioRxiv - Cell Biology 2020Quote: ... cDNA was retrotranscribed from 0.3 µg to 1 µg using the SensiFAST cDNA Synthesis Kit (Bioline, BIO-65054). Real-time PCR was performed on the CFX384 Touch Real-time PCR Detection System (Bio-Rad ...
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bioRxiv - Cell Biology 2021Quote: ... Bands of correct size were cut out and purified using the Isolate II PCR and gel kit (Bioline). 50 ng of vector and 150 ng of each fragment were mixed with 3 μl of HiFi DNA Master Mix (NEB ...
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bioRxiv - Molecular Biology 2019Quote: DNA was extracted from surface-sterilized whole insects using the ISOLATE II Genomic DNA Kit (Bioline, London, UK) according to the manufacturer’s instructions ...
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bioRxiv - Pathology 2019Quote: ... PCR amplicons of each sample were pulled together and purified using Isolate II PCR and gel Kit (BIOLINE) and about 100 ng of DNA was used in library preparation ...
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bioRxiv - Evolutionary Biology 2020Quote: ... DNA from historical museum toepad samples was extracted using the Bioline Isolate II Genomic DNA extraction kit (Bioline), but suspending tissues in 400 μL lysis buffer with 25 μL proteinase k and incubated overnight at 55 °C ...
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bioRxiv - Microbiology 2021Quote: ... Amplicons of appropriate size were excised and purified using an Isolate II PCR and Gel kit (Bioline, UK). The purified amplicons were included in cycle sequence reactions ...
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bioRxiv - Immunology 2021Quote: ... Real time quantitative PCR (RT-qPCR) analysis was carried out using the SensiMix SYBR No-Rox kit (Bioline), using primers and conditions shown in Supplemental Table 1 ...
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bioRxiv - Microbiology 2022Quote: Faecal DNA was extracted from FecalSwabTM media (200µL) using the ISOLATE II Fecal DNA Kit (Bioline, Sydney, Australia) following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2022Quote: ... Real-time PCR analysis was performed according to the manufacturer’s protocol (SensiMix™ SYBR® & Fluorescein Kit, Bioline) with a MyiQ Single-Color Real-Time PCR detection system (Bio-Rad ...