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Citations for Bioline :
551 - 600 of 761 citations for Human Tropomyosin alpha 4 chain TPM4 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pathology 2019Quote: ... PCR amplicons of each sample were pulled together and purified using Isolate II PCR and gel Kit (BIOLINE) and about 100 ng of DNA was used in library preparation ...
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bioRxiv - Evolutionary Biology 2020Quote: ... DNA from historical museum toepad samples was extracted using the Bioline Isolate II Genomic DNA extraction kit (Bioline), but suspending tissues in 400 μL lysis buffer with 25 μL proteinase k and incubated overnight at 55 °C ...
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bioRxiv - Microbiology 2021Quote: ... Amplicons of appropriate size were excised and purified using an Isolate II PCR and Gel kit (Bioline, UK). The purified amplicons were included in cycle sequence reactions ...
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bioRxiv - Immunology 2021Quote: ... Real time quantitative PCR (RT-qPCR) analysis was carried out using the SensiMix SYBR No-Rox kit (Bioline), using primers and conditions shown in Supplemental Table 1 ...
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bioRxiv - Microbiology 2022Quote: Faecal DNA was extracted from FecalSwabTM media (200µL) using the ISOLATE II Fecal DNA Kit (Bioline, Sydney, Australia) following the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2022Quote: Isolation of total RNA and cDNA synthesis was performed by using the Isolate II RNA Mini Kit (Bioline) and SensiFAST cDNA Synthesis Kit (Bioline) ...
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bioRxiv - Cell Biology 2022Quote: ... Real-time PCR analysis was performed according to the manufacturer’s protocol (SensiMix™ SYBR® & Fluorescein Kit, Bioline) with a MyiQ Single-Color Real-Time PCR detection system (Bio-Rad ...
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bioRxiv - Genetics 2022Quote: The relative amount of PCR product was quantified by qPCR using SensiFASTTM SYBR® Hi -ROX Kit (Bioline). The sequences of primers used for the quantitative detection of the chromosomal loci are listed in Suppl Table ST6 ...
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bioRxiv - Microbiology 2022Quote: ... conventional PCR assays were replaced with qPCR assays with SensiFAST™ SYBR® No-ROX Kit (Bioline, Australia). The cycling conditions included an initial denaturation at 95°C for 3 min followed by 28 -40 cycles of 95°C for 5 sec ...
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bioRxiv - Plant Biology 2023Quote: ... The total RNA was extracted from ground root samples using an ISOLATE II Plant Mini Kit (Bioline, Australia), cDNA was synthesised from 1ug of RNA using a SensiFAST cDNA Synthesis Kit (Bioline ...
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bioRxiv - Cancer Biology 2022Quote: ... transcripts were amplified directly on RNA samples using a one-step qRT-PCR kit with SYBR green (Bioline). Primers used against DDIT3 (CHOP ...
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bioRxiv - Neuroscience 2024Quote: ... and qPCR performed in technical triplicate using SensiFAST™ SYBR® Lo-ROX Kit following manufacturer instructions (Bioline) on QuantStudioTM 5 Real-Time PCR system as previously described (34,35,37,41) ...
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bioRxiv - Cancer Biology 2022Quote: ... cDNA was prepared for RT-qPCR using the SensiFAST cDNA preparation kit according to manufacturer instructions (Bioline #65054). 1μL of cDNA was used per RT-qPCR reaction prepared with SYBR Lo-ROX (Bioline #94020) ...
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bioRxiv - Molecular Biology 2023Quote: ... RNA was reverse-transcribed (1 mg each experimental point) by using SensiFAST cDNA Synthesis Kit (BIO-65053, Bioline) and qPCR was performed as described (18 ...
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bioRxiv - Genetics 2023Quote: ... 1 µg of RNA was used to prepare cDNA using the Sensifast cDNA synthesis kit (Bioline, London, UK). 2 µl aliquots of cDNA (corresponding to 20 ng of cDNA ...
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bioRxiv - Plant Biology 2023Quote: Measurements were carried out in 96-well plates using the SensiFAST™ SYBR® Kit (Bioline, Luckenwalde, Germany) in a LightCycler® 480 (Roche) ...
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bioRxiv - Evolutionary Biology 2023Quote: ... primers at a final concentration of 800nM and 6μL of SensiFAST™ SYBR® Lo-ROX Kit (Bioline). qPCR conditions were ...
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bioRxiv - Evolutionary Biology 2023Quote: ... 600 ng of isolated RNA was reverse transcribed to complementary DNA (cDNA) with SensiFAST cDNA Synthesis -kit (Bioline) following the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... cDNA was prepared for RT-qPCR using the SensiFAST cDNA preparation kit according to manufacturer instructions (Bioline #65054). 1µL of cDNA was used per RT-qPCR reaction prepared with SYBR Lo-ROX (Bioline #94020) ...
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Mapping chromatin state and transcriptional response in CIC-DUX4 undifferentiated round cell sarcomabioRxiv - Cancer Biology 2023Quote: ... Total RNA (1000 ng) was used in a reverse transcriptase reaction with the SensiFAST cDNA Synthesis Kit (Bioline). Quantitative PCR included 3 replicates per cDNA sample ...
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bioRxiv - Plant Biology 2024Quote: ... This template was then used to quantify relative mRNA abundance using the SensiMix SYBR Low-ROX kit (Bioline), a LightCycler® 480 (Roche ...
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bioRxiv - Cell Biology 2024Quote: ... 1μg of total RNA was used to synthesize cDNA using the SensiFAST cDNA Synthesis Kit (Bioline, BIO-65054) following manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... Correct sized amplicons were then purified using an Isolate II PCR and Gel Kit (Bioline, Meridian Biosciences, Australia) following the manufacturer’s instructions for PCR clean-up and sent for Sanger sequencing at the Victorian Clinical Genetics Services (VCGS ...
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bioRxiv - Molecular Biology 2024Quote: ... Genomic DNA was extracted from parasite lines using an Isolate II Genomic DNA Kit (Bioline, Meridian Bioscience Australia), following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... cells were collected by centrifugation at 4000 rpm for 15 minutes and pellets used for RNA extraction by RNeasy kit according to the manufacturer’s protocol (Bioline). cDNA synthesis and RT-qPCR was performed as previously described (60) ...
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bioRxiv - Cell Biology 2020Quote: ... Then 5 μg of the extracted RNA was reversely transcribed by utilizing Tetro cDNA Synthesis Kit (Bioline, BIO-65043) and random Hexamer Primer according to the manufacturer’s protocols ...
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bioRxiv - Genetics 2021Quote: ... RNA was reverse-transcribed (1 µg each experimental point) by using SensiFAST™ cDNA Synthesis Kit (BIO-65053, Bioline) and qPCR was performed using SensiFast Sybr Lo-Rox Mix (BIO-94020 ...
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bioRxiv - Developmental Biology 2021Quote: ... cDNA was made from 1 ug total RNA using Tetro cDNA synthesis kit with DNase treatment according to manufacturer’s instructions (Bioline). cDNA was diluted into 100 ul and 2 ul of cDNA was used to perform qRT-PCR ...
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bioRxiv - Zoology 2019Quote: ... The amplification was performed in a final volume of 12.5 μl using the SensiMix SYBR Low-ROX Kit (Bioline) with each primer at 200 nM ...
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bioRxiv - Plant Biology 2020Quote: ... 200 ng total RNA was used in each single-step reaction according to the manufacturer’s instruction (HiRoxSensifast Kit, Bioline). Primers (LHCSR3 fw/rev ...
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bioRxiv - Biochemistry 2021Quote: ... RT-qPCR was carried out using SensiMix™ SYBR® and Fluorescein kit (QT615-05; Bioline, London, United Kingdom). The following primers were used ...
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bioRxiv - Plant Biology 2020Quote: ... Real-time PCR reactions were performed in a 384-well microlitre using SensiFAST SYBR® No-ROX Kit (Bioline). The primers used here were designed using the open-source program QuantPrime-qPCR primer designed tool (Arvidsson et al. ...
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bioRxiv - Microbiology 2021Quote: ... After PCR verification in agarose gel and PCR clean-up using the ISOLATE II PCR and Gel Kit (Bioline), the remaining plasmid template was digested with DpnI at 37°C for 3h ...
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bioRxiv - Immunology 2022Quote: RNA was isolated from lung and blood using Isolate II RNA mini kit protocol (Bioline Reagents Ltd., London, UK) with slight modification ...
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bioRxiv - Molecular Biology 2020Quote: ... cDNA synthesis was carried out with 500 ng of RNA using the SensiFAST cDNA Synthesis Kit (Bioline, #BIO-65054) according to the manufacturer’s instructions.
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bioRxiv - Immunology 2019Quote: ... with first strand cDNA being synthesized from total RNA and reverse transcribed using a Tetro cDNA synthesis kit (Bioline). Quantitative real-time PCR was performed in a CFX Connect Real-Time Detection System (BioRad ...
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bioRxiv - Microbiology 2019Quote: ... with total reaction volume of 10 μl containing 5 μl Rotor-Gene SYBR® Green PCR Kit (Bioline, Australia), 0.3 μl each of 10 μM forward and reverse primer and 2 μl of genomic DNA ...
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bioRxiv - Plant Biology 2020Quote: ... First-strand cDNA was made from 2 μg of RNA using the Tetro cDNA synthesis kit with DNase treatment according to manufacturer’s instructions (Bioline). cDNA was diluted 1 to 5 into RNAse free water to a total of 100 μl ...
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bioRxiv - Microbiology 2021Quote: ... Genomic DNA was extracted from FecalSwab™ sample media (200μL) using the ISOLATE Fecal DNA kit (Bioline, Sydney, Australia) as per manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... Genomic DNA was DNA extracted from the sub-samples using the ISOLATE II Genomic DNA Extraction Kit (Bioline, UK) following the manufacturer’s instructions.
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bioRxiv - Microbiology 2020Quote: ... with first strand cDNA being synthesized from total RNA and reverse transcribed using a Tetro cDNA synthesis kit (Bioline). Quantitative realtime PCR was performed in a CFX Connect Real-Time Detection System (BioRad ...
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bioRxiv - Genetics 2020Quote: ... Genotyping was performed by PCR of genomic tail DNA using the Extract PCR kit (Bioline, cat. No. BIO-21127). All mice were kept on C57BL/6J (The Jackson Laboratory ...
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bioRxiv - Immunology 2021Quote: RNA was isolated from lung and blood using Isolate II RNA mini kit protocol (Bioline Reagents Ltd., London, UK) with slight modification ...
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bioRxiv - Cancer Biology 2021Quote: ... The relative amount of mRNA transcripts was measured by real-time PCR using SensiFAST SYBR N-ROX Kit (Bioline) and calculated by Bio-Rad CFX Manager Software ...
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bioRxiv - Microbiology 2020Quote: ... After three washes with ice-cold PBS genomic DNA was isolated using the ISOLATE II Genomic DNA Kit (Bioline) according to manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2021Quote: ... cDNA was 1:5 diluted and 0.5 µl were used per 10µl qPCR reaction with SensiFAST™ SYBR(R) No-ROX kit (Bioline) and 125 nM forward and reverse primer ...
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bioRxiv - Genetics 2020Quote: ... cDNA was diluted 16-fold with deionized water and amplified using the SensiFAST SYBR Lo-ROX kit (Bioline, USA) for qRT-PCR analysis ...
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bioRxiv - Plant Biology 2022Quote: ... PCR products were purified from agarose gels using the ISOLATE II PCR and Gel Kit (Bioline, Memphis, TN, USA), and quantified in a NanoDrop One C Spectrophotometer (Thermo Scientific ...
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bioRxiv - Synthetic Biology 2022Quote: ... supplemented with 100 μg/ml ampicillin were processed with either the ISOLATE II Plasmid Mini Kit (Bioline, #BIO-52057) for sequence verification ...
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bioRxiv - Physiology 2022Quote: ... qPCRs were carried out in 15 μl in a 384-well plate using SensiFASTTM SYBR Hi-ROX kit (Bioline), 0·5 μM of each forward and reverse primer and 2·5 μl of template DNA ...