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Citations for Bioline :
501 - 550 of 732 citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... RT-PCR reaction was setup using SensiFast Probe No-ROX One- Step Kit (Bioline, Cat. BIO-76005) using the following primers/probes ...
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bioRxiv - Cancer Biology 2023Quote: Total RNA was extracted from PCa cells using the Isolate II RNA Mini Kit (Bioline, London, UK) and was reverse transcribed to cDNA using the SensiFastTM cDNA synthesis kit (Bioline ...
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bioRxiv - Immunology 2023Quote: ... and on-column DNase treatment and purification were performed using the ISOLATE II RNA mini kit (Bioline) or RNeasy Mini kit (Qiagen ...
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bioRxiv - Evolutionary Biology 2023Quote: ... qRT-PCR was carried out on 1:10 diluted cDNA using Sensifast Hi-Rox Sybr kit (Bioline). Two qRT-PCR reactions (technical replicates ...
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Quantitative Comparison of Presenilin Protein Expression Reveals Greater Activity of PS2-γ-SecretasebioRxiv - Cell Biology 2023Quote: ... 0.5 µg of RNA was used to generate cDNA using Tetro cDNA synthesis kit (Bioline BIO-65043) in a 10 µl reaction using a 1:1 ratio of random hexamer and oligo (dT)18 primer mix as per manufacturer instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... and 1 µg of RNA was reverse transcribed to cDNA using SensiFAST cDNA Synthesis Kit (Bioline #65054). qPCR reactions were set up using PowerUp SYBR Green Master Mix (Applied Biosystems #A25742 ...
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bioRxiv - Cancer Biology 2024Quote: Quantitative RT-PCR analyses were performed in technical triplicates with SensiMix SYBR kit (Bioline, Cat# QT605–20) using the ViiA™ 7 Real Time PCR System (Life Technologies) ...
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bioRxiv - Microbiology 2024Quote: ... qRT-PCR analysis was performed on 50 ng of cDNA using the SensiFAST SYBR & Fluorescein kit (Bioline) with a Roche Lightcycler 96 ...
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bioRxiv - Cancer Biology 2024Quote: ... and 500 ng RNA per sample was used as input to the SensiFAST cDNA Synthesis Kit (Bioline), including -RT controls ...
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bioRxiv - Cell Biology 2020Quote: Total RNA was extracted from treated cells via an ISOLATE II RNA mini kit (Bioline, Cat: BIO-52073) following manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... Purified RNA (500 ng) was transcribed into cDNA with Tetro cDNA Synthesis Kit (Bioline Reagents Limited, London, UK) to analyze genes expression ...
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bioRxiv - Cancer Biology 2022Quote: ... The purified DNAs were amplified with SYBR Green SensiFAST™ SYBR® No-ROX Kit (Bioline, London, UK) by real-time qPCR.
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bioRxiv - Molecular Biology 2021Quote: ... qPCR quantification of DNA samples was carried out using the SensiFAST SYBR Hi-ROX Kit (Bioline, BIO-92005) in 96-well plates using a ABI PRISM 7000 system (Applied Biosystems) ...
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bioRxiv - Microbiology 2020Quote: ... PCR products were purified using the Bioline Isolate II PCR and Gel kit (Bioline, Cat. No. BIO-52060). Samples were sequenced using the Illumina Miseq platform ...
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bioRxiv - Cell Biology 2020Quote: ... cDNA was retrotranscribed from 0.3 µg to 1 µg using the SensiFAST cDNA Synthesis Kit (Bioline, BIO-65054). Real-time PCR was performed on the CFX384 Touch Real-time PCR Detection System (Bio-Rad ...
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bioRxiv - Plant Biology 2020Quote: ... RNA was used for reverse transcription according to the protocol of the SensiFASTtm cDNA synthesis kit from Bioline. PCR reactions were carried out with 5 μL of 100x diluted cDNA solution ...
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bioRxiv - Microbiology 2020Quote: ... cDNA was synthesised using SuperScript III reverse transcriptase from RNA extracted using ISOLATE II RNA Mini Kit (Bioline) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... Bands of correct size were cut out and purified using the Isolate II PCR and gel kit (Bioline). 50 ng of vector and 150 ng of each fragment were mixed with 3 μl of HiFi DNA Master Mix (NEB ...
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bioRxiv - Cancer Biology 2021Quote: ... The conversion of messenger RNA into cDNA was performed using the Sensifast cDNA synthesis kit (Bioline, BIO- 65053). cDNA was then amplified by PCR using specific primers for each gene designed with Primer-blast software (https://www.ncbi.nlm.nih.gov/tools/primer-blast/ ...
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bioRxiv - Genetics 2021Quote: ... 1 µg of total RNA was reverse transcribed using SensiFAST cDNA Synthesis Kit following the manufacturer’s protocol (Bioline).
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bioRxiv - Plant Biology 2021Quote: ... Reverse transcription was performed in 20mL with 1mg of total RNA using the Tetro cDNA synthesis kit (Bioline). RT-negative (no enzyme ...
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bioRxiv - Microbiology 2021Quote: ... 1:10 dilutions of RNA samples were analyzed using SensiFast SYBR Hi-ROX One-Step kit (Bioline, UK) and 400 nM primer concentrations ...
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bioRxiv - Microbiology 2021Quote: ... Two micrograms of total RNA was reverse-transcribed with Sensi-FAST™ cDNA Synthesis Kit (Bioline, Memphis, USA). The amounts of p16 ...
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bioRxiv - Evolutionary Biology 2020Quote: ... DNA from historical museum toepad samples was extracted using the Bioline Isolate II Genomic DNA extraction kit (Bioline), but suspending tissues in 400 μL lysis buffer with 25 μL proteinase k and incubated overnight at 55 °C ...
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bioRxiv - Microbiology 2021Quote: ... Amplicons of appropriate size were excised and purified using an Isolate II PCR and Gel kit (Bioline, UK). The purified amplicons were included in cycle sequence reactions ...
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bioRxiv - Immunology 2021Quote: ... Real time quantitative PCR (RT-qPCR) analysis was carried out using the SensiMix SYBR No-Rox kit (Bioline), using primers and conditions shown in Supplemental Table 1 ...
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bioRxiv - Cancer Biology 2022Quote: ... cDNA was prepared for RT-qPCR using the SensiFAST cDNA preparation kit according to manufacturer instructions (Bioline #65054). 1μL of cDNA was used per RT-qPCR reaction prepared with SYBR Lo-ROX (Bioline #94020) ...
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bioRxiv - Molecular Biology 2023Quote: ... RNA was reverse-transcribed (1 mg each experimental point) by using SensiFAST cDNA Synthesis Kit (BIO-65053, Bioline) and qPCR was performed as described (18 ...
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bioRxiv - Genetics 2023Quote: ... 1 µg of RNA was used to prepare cDNA using the Sensifast cDNA synthesis kit (Bioline, London, UK). 2 µl aliquots of cDNA (corresponding to 20 ng of cDNA ...
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bioRxiv - Plant Biology 2023Quote: Measurements were carried out in 96-well plates using the SensiFAST™ SYBR® Kit (Bioline, Luckenwalde, Germany) in a LightCycler® 480 (Roche) ...
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bioRxiv - Cancer Biology 2022Quote: ... transcripts were amplified directly on RNA samples using a one-step qRT-PCR kit with SYBR green (Bioline). Primers used against DDIT3 (CHOP ...
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bioRxiv - Neuroscience 2024Quote: ... and qPCR performed in technical triplicate using SensiFAST™ SYBR® Lo-ROX Kit following manufacturer instructions (Bioline) on QuantStudioTM 5 Real-Time PCR system as previously described (34,35,37,41) ...
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bioRxiv - Molecular Biology 2023Quote: ... cDNA was prepared for RT-qPCR using the SensiFAST cDNA preparation kit according to manufacturer instructions (Bioline #65054). 1µL of cDNA was used per RT-qPCR reaction prepared with SYBR Lo-ROX (Bioline #94020) ...
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Mapping chromatin state and transcriptional response in CIC-DUX4 undifferentiated round cell sarcomabioRxiv - Cancer Biology 2023Quote: ... Total RNA (1000 ng) was used in a reverse transcriptase reaction with the SensiFAST cDNA Synthesis Kit (Bioline). Quantitative PCR included 3 replicates per cDNA sample ...
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bioRxiv - Plant Biology 2024Quote: ... This template was then used to quantify relative mRNA abundance using the SensiMix SYBR Low-ROX kit (Bioline), a LightCycler® 480 (Roche ...
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bioRxiv - Microbiology 2022Quote: Faecal DNA was extracted from FecalSwabTM media (200µL) using the ISOLATE II Fecal DNA Kit (Bioline, Sydney, Australia) following the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2022Quote: Isolation of total RNA and cDNA synthesis was performed by using the Isolate II RNA Mini Kit (Bioline) and SensiFAST cDNA Synthesis Kit (Bioline) ...
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bioRxiv - Cell Biology 2022Quote: ... Real-time PCR analysis was performed according to the manufacturer’s protocol (SensiMix™ SYBR® & Fluorescein Kit, Bioline) with a MyiQ Single-Color Real-Time PCR detection system (Bio-Rad ...
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bioRxiv - Genetics 2022Quote: The relative amount of PCR product was quantified by qPCR using SensiFASTTM SYBR® Hi -ROX Kit (Bioline). The sequences of primers used for the quantitative detection of the chromosomal loci are listed in Suppl Table ST6 ...
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bioRxiv - Plant Biology 2023Quote: ... The total RNA was extracted from ground root samples using an ISOLATE II Plant Mini Kit (Bioline, Australia), cDNA was synthesised from 1ug of RNA using a SensiFAST cDNA Synthesis Kit (Bioline ...
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bioRxiv - Evolutionary Biology 2023Quote: ... primers at a final concentration of 800nM and 6μL of SensiFAST™ SYBR® Lo-ROX Kit (Bioline). qPCR conditions were ...
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bioRxiv - Evolutionary Biology 2023Quote: ... 600 ng of isolated RNA was reverse transcribed to complementary DNA (cDNA) with SensiFAST cDNA Synthesis -kit (Bioline) following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2024Quote: ... 1μg of total RNA was used to synthesize cDNA using the SensiFAST cDNA Synthesis Kit (Bioline, BIO-65054) following manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... Genomic DNA was extracted from parasite lines using an Isolate II Genomic DNA Kit (Bioline, Meridian Bioscience Australia), following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... Correct sized amplicons were then purified using an Isolate II PCR and Gel Kit (Bioline, Meridian Biosciences, Australia) following the manufacturer’s instructions for PCR clean-up and sent for Sanger sequencing at the Victorian Clinical Genetics Services (VCGS ...
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bioRxiv - Microbiology 2020Quote: ... cells were collected by centrifugation at 4000 rpm for 15 minutes and pellets used for RNA extraction by RNeasy kit according to the manufacturer’s protocol (Bioline). cDNA synthesis and RT-qPCR was performed as previously described (60) ...
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bioRxiv - Cell Biology 2020Quote: ... Then 5 μg of the extracted RNA was reversely transcribed by utilizing Tetro cDNA Synthesis Kit (Bioline, BIO-65043) and random Hexamer Primer according to the manufacturer’s protocols ...
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bioRxiv - Genetics 2021Quote: ... RNA was reverse-transcribed (1 µg each experimental point) by using SensiFAST™ cDNA Synthesis Kit (BIO-65053, Bioline) and qPCR was performed using SensiFast Sybr Lo-Rox Mix (BIO-94020 ...
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bioRxiv - Developmental Biology 2021Quote: ... cDNA was made from 1 ug total RNA using Tetro cDNA synthesis kit with DNase treatment according to manufacturer’s instructions (Bioline). cDNA was diluted into 100 ul and 2 ul of cDNA was used to perform qRT-PCR ...
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bioRxiv - Plant Biology 2020Quote: ... 200 ng total RNA was used in each single-step reaction according to the manufacturer’s instruction (HiRoxSensifast Kit, Bioline). Primers (LHCSR3 fw/rev ...