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1 - 50 of 62 citations for Recombinant Human High Mobility Group Box 1 His tagged since 2019

• Human NAIP/NLRC4 and NLRP3 inflammasomes detect Salmonella type III secretion system activities to restrict intracellular bacterial replication

  Nawar Naseer, et al., bioRxiv - Microbiology 2021

  Quote: ... and 50 ng/ml recombinant human M-CSF (Gemini Bio-Products) for 6 days to promote differentiation into hMDMs ...
• Inflammasomes primarily restrict cytosolic Salmonella replication within human macrophages

  Marisa S. Egan, et al., bioRxiv - Microbiology 2023

  Quote: ... and 50 ng/ml recombinant human M-CSF (Gemini Bio-Products). Cells were cultured for 4 days in 10 mL of media in at a concentration of 4-5 × 105 cells/mL in 10 cm-dishes ...
• Yersinia Type III-Secreted Effectors Evade the Caspase-4 Inflammasome in Human Cells

  Jenna Zhang, Igor E. Brodsky, Sunny Shin, bioRxiv - Microbiology 2023

  Quote: ... and 50 ng/ml recombinant human M-CSF (Gemini Bio-Products) for 6 days ...
• TLR priming licenses NAIP inflammasome activation by immunoevasive ligands

  James P. Grayczyk, et al., bioRxiv - Molecular Biology 2023

  Quote: ... heat inactivated FBS + 2 mM L-glutamine + 100 IU/mL penicillin + 100 μg/mL streptomycin + 50 ng/mL recombinant human M-CSF (Gemini Bio-Products) for 6 days at 37°C ...
• Regulation of CNS regeneration-associated genes is driven by a temporally changing cast of transcription factors

  Sumona P. Dhara, et al., bioRxiv - Neuroscience 2019

  Quote: ... Reactions were quenched in heat inactivated fetal calf serum (HI-FCS; Gemini Bio-Products) in DMEM/F12 (Gibco ...
• Intra-peritoneal transplantation for efficient and easy generation of experimental acute myeloid leukemia in mice

  Fenghua Qian, et al., bioRxiv - Cancer Biology 2022

  Quote: ... 25 ng/mL mouse recombinant (mr) stem cell factor (Gemini bio-products), 25 ng/mL mrFlt3L (Pepro Tech) ...
• Genome-wide characterization of T cell responses to Bordetella pertussis reveals broad reactivity and similar polarization irrespective of childhood vaccination profiles

  Ricardo da Silva Antunes, et al., bioRxiv - Immunology 2023

  Quote: ... in 5% human serum (Gemini Bio-Products) for 24 h ...
• Oleic acid Induces Tissue Resident FoxP3 Regulatory T cell Lineage Stability and Suppressive Functions

  Saige Lorraine Pompura, et al., bioRxiv - Immunology 2020

  Quote: ... and 5% human AB serum (Gemini Bio-Products). 96-well round bottom plates (Corning ...
• Type I Interferon Transcriptional Network Regulates Expression of Coinhibitory Receptors in Human T cells

  Tomokazu S. Sumida, et al., bioRxiv - Immunology 2020

  Quote: ... and 5% human AB serum (Gemini Bio-Products). Cells were seeded (30,000-50,000/wells ...
• An Autoimmune Transcriptional Circuit Driving Foxp3⁺ Regulatory T cell Dysfunction

  Tomokazu S. Sumida, et al., bioRxiv - Immunology 2022

  Quote: ... and 5% human AB serum (Gemini Bio-Products). Cells were seeded (30,000-50,000/well ...
• CD47 cross-dressing by extracellular vesicles expressing CD47 inhibits phagocytosis without transmitting cell death signals

  Yang Li, et al., bioRxiv - Immunology 2021

  Quote: ... supplemented with 10% AB human serum (Gemini Bio-products, Inc.) containing 10ng/ml human M-CSF (PeproTech ...
• Antigen-driven CD8⁺ T cell clonal expansion is a prominent feature of MASH in humans and mice

  Abbigayl E.C. Burtis, et al., bioRxiv - Immunology 2024

  Quote: ... containing 10% Human Serum AB (Gemini Bio-products, West Sacramento, CA) and 1% DNASE (MP Biomedicals ...
• Differential effects of the second SARS-CoV-2 mRNA vaccine dose on T cell immunity in naïve and COVID-19 recovered individuals

  Carmen Camara, et al., bioRxiv - Immunology 2021

  Quote: ... with 2.5% AB human serum (Gemini Bio-Products, Inc., West Sacramento, CA). PBMCs were unstimulated (DMSO ...
• Mycobacterium tuberculosis-specific CD4 T cells expressing transcription factors associate with bacterial control in granulomas

  Nicole L. Grant, et al., bioRxiv - Immunology 2022

  Quote: ... and 10% human A/B serum (Gemini Bio-Products, Cat. #100-512). Peptides were added to prepared plates at a final concentration of 1μg/mL-2μg/mL followed by 1x105-2x105 fresh or frozen (rested overnight ...
• Defining antigen targets to dissect vaccinia virus (VACV) and Monkeypox virus (MPXV)-specific T cell responses in humans

  Alba Grifoni, et al., bioRxiv - Immunology 2022

  Quote: ... and blocked for 15 minutes with 10% human serum (Gemini Bio-Products, Sacramento, CA) in saponin buffer ...
• SARS-CoV-2 mRNA vaccination elicits robust and persistent T follicular helper cell response in humans

  Philip A. Mudd, et al., bioRxiv - Immunology 2021

  Quote: ... suspended in RPMI 1640 supplemented with 10% human AB serum (Gemini Bio-Products, 100-512), 100 U/mL penicillin-streptomycin ...
• Single-genome analysis reveals heterogeneous association of the Herpes Simplex Virus genome with H3K27me2 and the reader PHF20L1 following infection of human fibroblasts

  Alison K Francois, et al., bioRxiv - Microbiology 2023

  Quote: ... and cultured with 5 ng/ml human fibroblast growth factor (FGF, Gemini Bio-Products 300-113P) throughout clonal selection ...
• CD4+CCR6+ T cells dominate the BCG-induced transcriptional signature

  Akul Singhania, et al., bioRxiv - Immunology 2021

  Quote: ... cryopreserved PBMCs were thawed in RPMI supplemented with 5% human serum (Gemini Bio-Products, West Sacramento, CA), 1% Glutamax (Gibco ...
• CD4+CCR6+ T cells dominate the BCG-induced transcriptional signature

  Akul Singhania, et al., bioRxiv - Immunology 2021

  Quote: ... Cryopreserved PBMCs were thawed in RPMI supplemented with 5% human serum (Gemini Bio-Products, West Sacramento, CA), 1% Glutamax (Gibco,Waltham,MA) ...
• Oncolytic virus treatment differentially affects the CD56^dim and CD56^bright NK cell subsets in vivo and regulates a spectrum of human NK cell activity

  Michelle Wantoch, et al., bioRxiv - Immunology 2020

  Quote: ... NK cells were cultured in Dulbecco’s Modified Eagle Medium (DMEM) supplemented with 10% human AB serum (Gemini Bio-Products) and 10% FCS at 37°C ...
• Dissecting the heterogeneity of DENV vaccine-elicited cellular immunity using single-cell RNA sequencing and cellular metabolic profiling

  Adam T. Waickman, et al., bioRxiv - Immunology 2019

  Quote: Cryopreserved PBMC were thawed and placed in RPMI 1640 medium supplemented with 10% heat-inactivated normal human serum (100-318, Gemini Bio-Products), L-glutamine ...
• Analysis of cell-associated DENV RNA by oligo(dT) primed 5' capture scRNAseq

  Mark A Sanborn, et al., bioRxiv - Microbiology 2020

  Quote: Cryopreserved PBMC were thawed and placed in RPMI 1640 medium supplemented with 10% heat-inactivated normal human serum (100–318, Gemini Bio-Products), L-glutamine ...
• Transcriptional analysis of peripheral memory T cells reveals Parkinson’s disease-specific gene signatures

  Rekha Dhanwani, et al., bioRxiv - Immunology 2021

  Quote: The cryopreserved PBMC were thawed and revived in prewarmed RPMI media supplemented with 5% human serum (Gemini Bio-Products, West Sacramento, CA), 1 % Glutamax (Gibco,Waltham ...
• All-optical electrophysiology in hiPSC-derived neurons with synthetic voltage sensors

  Francesca Puppo, et al., bioRxiv - Neuroscience 2021

  Quote: ... and 1% Penicillin streptomycin) supplemented with 1% fetal bovine serum (FBS, Gemini Bio-products), 1 μg/ml of laminin and 0.1% ROCK (Rho kinase ...
• Rescue of alveolar wall liquid secretion blocks fatal lung injury by influenza-staphylococcal coinfection

  Stephanie Tang, et al., bioRxiv - Physiology 2022

  Quote: ... 1% FBS (Gemini Bio-Products), and HEPES-buffered solution at pH 7.4 ...
• Sphingomyelin suppresses Hedgehog signaling by restricting cholesterol accessibility at the ciliary membrane

  Maia Kinnebrew, et al., bioRxiv - Cell Biology 2019

  Quote: ... 1% penicillin/streptomycin (Gemini Bio-Products), 2mM L-glutamine (Gemini Biosciences) ...
• NVX-CoV2373 vaccination induces functional SARS-CoV-2–specific CD4⁺ and CD8⁺ T cell responses

  Carolyn Rydyznski Moderbacher, et al., bioRxiv - Immunology 2022

  Quote: ... 1% penicillin/streptomycin)(Gemini Bio-products) and 2mM L-glutamine (Gibco ...
• Intra-peritoneal transplantation for efficient and easy generation of experimental acute myeloid leukemia in mice

  Fenghua Qian, et al., bioRxiv - Cancer Biology 2022

  Quote: ... 1% bovine serum albumin (Gemini bio-products), 10 mg/mL insulin (Sigma) ...
• SARS-CoV-2 nucleocapsid protein forms condensates with viral genomic RNA

  Amanda Jack, et al., bioRxiv - Biophysics 2021

  Quote: ... and 1% penicillin-streptomycin (Gemini Bio-products) to 2 million cells/mL ...
• Self-assembling short immunostimulatory duplex RNAs with broad spectrum antiviral activity

  Longlong Si, et al., bioRxiv - Immunology 2021

  Quote: ... 1% (v/v) penicillin/streptomycin (Gemini Bio-products) and 1% (v/v ...
• Human organ chip-enabled pipeline to rapidly repurpose therapeutics during viral pandemics

  Longlong Si, et al., bioRxiv - Microbiology 2020

  Quote: ... 1% (v/v) penicillin/streptomycin (Gemini Bio-products), and 1% (v/v ...
• Broad anti-coronaviral activity of FDA approved drugs against SARS-CoV-2 in vitro and SARS-CoV in vivo

  Stuart Weston, et al., bioRxiv - Microbiology 2020

  Quote: ... 1% (v/v) penicillin/streptomycin (Gemini Bio-products) and 1% (v/v ...
• An adjuvanted SARS-CoV-2 RBD nanoparticle elicits neutralizing antibodies and fully protective immunity in aged mice

  Francesco Borriello, et al., bioRxiv - Immunology 2021

  Quote: ... 1% (v/v) penicillin/streptomycin (Gemini Bio-products) and 1% (v/v ...
• Immunogenicity and In vivo protection of a variant nanoparticle vaccine that confers broad protection against emerging SARS-CoV-2 variants

  James Logue, et al., bioRxiv - Immunology 2021

  Quote: ... 1% (v/v) penicillin/streptomycin (Gemini Bio-products) and 1% (v/v ...
• Alum:CpG adjuvant enables SARS-CoV-2 RBD-induced protection in aged mice and synergistic activation of human elder type 1 immunity

  Etsuro Nanishi, et al., bioRxiv - Immunology 2021

  Quote: ... 1% (v/v) penicillin/streptomycin (Gemini Bio-products) and 1% (v/v ...
• Reduced SARS-CoV-2 mRNA vaccine immunogenicity and protection in mice with diet-induced obesity and insulin resistance

  Timothy R. O’Meara, et al., bioRxiv - Immunology 2022

  Quote: ... 1% (v/v) penicillin/streptomycin (Gemini Bio-products) and 1% (v/v ...
• Diet Induced Obesity and Diabetes Enhance Mortality and Reduces Vaccine Efficacy for SARS-CoV-2

  Robert M Johnson, et al., bioRxiv - Microbiology 2023

  Quote: ... 1% (v/v) penicillin/streptomycin (Gemini Bio-products), and 1% (v/v ...
• Diet Induced Obesity and Diabetes Enhance Mortality and Reduces Vaccine Efficacy for SARS-CoV-2

  Robert M Johnson, et al., bioRxiv - Microbiology 2023

  Quote: ... 1% (v/v) penicillin/streptomycin (Gemini Bio-products) and 1% (v/v ...
• Metastatic breast cancer cells have reduced calcium and actin response after ATP-P2Y2 signaling

  Makenzy L. Mull, et al., bioRxiv - Cancer Biology 2023

  Quote: ... 1% Pen/Strep (Gemini Bio-products, 400-109), Recombinant Human EGF (Invitrogen ...
• Single cell susceptibility to SARS-CoV-2 infection is driven by variable cell states

  Sam Reffsin, et al., bioRxiv - Bioengineering 2023

  Quote: ... 1% (v/v) penicillin–streptomycin (Gemini Bio-products) and 1% (v/v ...
• SIRT-1 is required for release of enveloped enteroviruses

  Alagie Jassey, et al., bioRxiv - Microbiology 2023

  Quote: ... 1% (vol/vol) penicillin-streptomycin (Gemini Bio-Products) and 1% (vol/vol ...
• CNP blocks mitochondrial depolarization and inhibits SARS-CoV-2 replication in vitro and in vivo

  James Logue, et al., bioRxiv - Microbiology 2023

  Quote: ... 1% (vol/vol) penicillin–streptomycin (Gemini Bio-Products), 1% (vol/vol ...
• Tunable Fibrin-Alginate Interpenetrating Network Hydrogels to Guide Cell Behavior

  Charlotte E. Vorwald, et al., bioRxiv - Bioengineering 2019

  Quote: ... and 1% penicillin/streptomycin (Gemini Bio-Products, Sacramento, CA). MSCs were cultured under standard conditions until use at passage 5 ...
• Human CD8⁺ T-cells Require Glycolysis to Elicit Effector Function

  Scott E. Stimpson, et al., bioRxiv - Immunology 2020

  Quote: ... 1% penicillin-streptomycin (Gemini Bio-Products, West Sacramento, CA) solution ...
• Human CD8⁺ T-cells Require Glycolysis to Elicit Effector Function

  Scott E. Stimpson, et al., bioRxiv - Immunology 2020

  Quote: ... 1% penicillin-streptomycin (Gemini Bio-Products, West Sacramento, CA) solution ...
• A new pipeline SPICE identifies novel JUN-IKZF1 composite elements

  Peng Li, et al., bioRxiv - Systems Biology 2023

  Quote: ... and 1% penicillin-streptomycin (Gemini Bio-Products, 400-109). Mouse splenic B cells were isolated using the EasySepTM Mouse B cell isolation Kit (STEMCELL technologies ...
• SIRT-1 is required for release of enveloped enteroviruses

  Alagie Jassey, et al., bioRxiv - Microbiology 2023

  Quote: ... and 1% (vol/vol) penicillin-streptomycin (Gemini Bio-Products). For induction of autophagy by starvation before infection ...
• Interferon-Induced Transmembrane Protein 3 Blocks Fusion of Diverse Enveloped Viruses by Locally Altering Mechanical Properties of Cell Membranes

  Xiangyang Guo, et al., bioRxiv - Biophysics 2020

  Quote: ... and 1% penicillin/streptomycin (Gemini Bio-products, West Sacramento, CA). For HEK 293T/17 cells ...
• Evidence for in vitro extensive proliferation of adult hepatocytes and biliary epithelial cells

  Takeshi Katsuda, et al., bioRxiv - Cell Biology 2023

  Quote: ... and 1× antibiotics (anti-anti (Thermo) or gentamycin (Gemini Bio-Products)) ...
• Neurons burdened by DNA double strand breaks incite microglia activation through antiviral-like signaling in neurodegeneration

  Gwyneth Welch, et al., bioRxiv - Neuroscience 2021

  Quote: ... 1% Penicillin/Streptomycin Solution (cat no. 400-109, Gemini Bio-Products, Sacramento CA), 10% FBS) ...