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• Paratope mapping of tilvestamab, an anti-AXL function blocking antibody, using high-throughput bacterial expression of secreted scFv-ompY fusion proteins

  Eleni Christakou, Petri Kursula, David Micklem, bioRxiv - Biochemistry 2024

  Quote: ... joined together by a short flexible peptide linker AKTTPPKLEEGEFSEARV (adapted from the vector pOPE101, Progen, Genbank, accession Y14585). The scFv was connected by a short linker RADAAPTVSAA to the gene coding for the bacterial osmotically inducible protein Y (osmY) ...
• Piezo1 balances membrane tension and cortical contractility to stabilize intercellular junctions and maintain epithelial barrier integrity

  Ahsan Javed, et al., bioRxiv - Cell Biology 2024

  Quote: ... guineapig polyclonal anti-Keratin K14 (Progen GP-CK14; 1:500), rat monoclonal anti-F4/80 (BMA Biomedicals T-2028 ...
• Small-molecule inhibitor of C-terminal HSP90 dimerization modulates autophagy and functions synergistically with mTOR inhibition to kill cisplatin-resistant cancer cells

  Céline David, et al., bioRxiv - Cell Biology 2024

  Quote: ... primary antibodies against SQSTM1/p62 (PROGEN Biotechnik, #GP62-C), LC3 (Cell Signaling Technology ...
• Molecular and Mechanical Signatures Contributing to Epidermal Differentiation and Barrier Formation

  Alexandra Prado-Mantilla, et al., bioRxiv - Developmental Biology 2024

  Quote: ... guinea pig anti-keratin 10 (Progen, GP-K10), rat anti-β4 integrin (BD Biosciences ...
• Transcription arrest induces formation of protective RNA granules in mitochondria

  Katja G. Hansen, et al., bioRxiv - Cell Biology 2024

  Quote: ... ds/ssDNA (PROGEN, #61014) used in a 1:100 dilution ...
• Chronic metabolic stress drives developmental programs and loss of tissue functions in non-transformed liver that mirror tumor states and stratify survival

  Constantine N. Tzouanas, et al., bioRxiv - Systems Biology 2023

  Quote: ... A primary antibody already targeting K8/18 was used instead of mixing K8 & K18 1:1 (Progen, GP11; 1:400), detected with ImmPACT AMEC Red per manufacturer instructions (Vector Laboratories ...
• Spatial genomics of AAVs reveals mechanism of transcriptional crosstalk that enables targeted delivery of large genetic cargo

  Gerard M. Coughlin, et al., bioRxiv - Bioengineering 2023

  Quote: ... a mouse anti-AAV VP1/VP2/VP3 monoclonal antibody conjugated to Alexa Fluor 488 was used (Clone B1, Progen, #61059-488). Following poly(dT ...
• Rupture Strength of Living Cell Monolayers

  Julia Duque, et al., bioRxiv - Biophysics 2023

  Quote: ... The antibodies used were anti-desmoplakin (Progen) and with anti-GAPDH as a loading control (Abcam) ...
• A fluorogenic complementation tool kit for interrogating lipid droplet-organelle interaction

  Xiao Li, et al., bioRxiv - Cell Biology 2023

  Quote: ... anti-Plin 5 (Progen Cat# GP31), anti-VAPA (Sigma Cat# HPA009174) ...
• Electrostatic interactions control the adsorption of extracellular vesicles onto supported lipid bilayers

  Andrea Ridolfi, et al., bioRxiv - Biophysics 2023

  Quote: ... 6.5 ml of the milk 10000 xg supernatant was loaded on top of a 60% – 10% Optiprep gradient (OptiprepTM, Progen Biotechnik GmbH, Heidelberg, Germany) made in a SW40 tube ...
• New insights underlying the early events of dopaminergic dysfunction in Parkinson’s Disease

  Hannah L. Dela Cruz, et al., bioRxiv - Neuroscience 2023

  Quote: ... The sections were immunostained with the primary antibody: 1/500 diluted guinea pig anti-p62/SQSTM-1 (ProGen, GP62-C) and 1/1500 chicken anti-tyrosine hydroxylase (Encor ...
• Calcium signals tune AMPK activity and mitochondrial homeostasis in dendrites of developing neurons

  Akane Hatsuda, et al., bioRxiv - Neuroscience 2023

  Quote: ... hippocampal neurons transfected with GFP and mito-DsRed were fixed at DIV5 and immunostained with guinea pig anti-p62 primary antibody (1:100, Progen, GP62-C,) and goat anti-Guinea Pig Alexa Fluor 633 secondary antibody (1:400 ...
• DPM1 through SERPINB5 modulates desmosomal adhesion and epidermal differentiation

  Maitreyi Rathod, et al., bioRxiv - Cell Biology 2023

  Quote: ... mouse DSG1 ((#651111 Progen), rabbit keratin 10 (#905403 Biolegend) ...
• DPM1 through SERPINB5 modulates desmosomal adhesion and epidermal differentiation

  Maitreyi Rathod, et al., bioRxiv - Cell Biology 2023

  Quote: ... rabbit Dsg2 pAb (#610121 Progen), mouse Dsg3 mAb (clone 5G11 ...
• DPM1 through SERPINB5 modulates desmosomal adhesion and epidermal differentiation

  Maitreyi Rathod, et al., bioRxiv - Cell Biology 2023

  Quote: ... mouse Pkp2 mAb (#651101 Progen, Heidelberg, Germany), mouse PG mAb (clone PG5.1 ...
• DPM1 through SERPINB5 modulates desmosomal adhesion and epidermal differentiation

  Maitreyi Rathod, et al., bioRxiv - Cell Biology 2023

  Quote: ... The following primary antibodies were incubated in PBS at 4°C overnight: mouse DSP (#61003 Progen), rabbit DSG2 RB5 (# 610120 Progen Biotechnik) ...
• DPM1 through SERPINB5 modulates desmosomal adhesion and epidermal differentiation

  Maitreyi Rathod, et al., bioRxiv - Cell Biology 2023

  Quote: ... rabbit DSG2 RB5 (# 610120 Progen Biotechnik), Filaggrin (#ab218395 Abcam) ...
• DPM1 through SERPINB5 modulates desmosomal adhesion and epidermal differentiation

  Maitreyi Rathod, et al., bioRxiv - Cell Biology 2023

  Quote: ... mouse PG mAb (clone PG5.1, #61005 Progen), mouse DSP mAb (#sc-390975 Santa Cruz) ...
• HER2 overexpression initiates breast tumorigenesis non-cell-autonomously by inducing oxidative stress in the tissue microenvironment

  Sevim B. Gurler, et al., bioRxiv - Cancer Biology 2023

  Quote: ... anti-Cytokeratin-18 (61028, Progen), anti-cleaved caspase 3 (#9661 ...
• TECPR1 provides E3-ligase like activity to the ATG5-ATG12 complex to conjugate LC3/ATG8 to damaged lysosomes

  Yingxue Wang, et al., bioRxiv - Cell Biology 2023

  Quote: ... GP-p62 (Progen GP62-C), mouse-EEA1 (MBL M176-3) ...
• Expression of ALS-PFN1 impairs vesicular degradation in iPSC-derived microglia

  Salome Funes, et al., bioRxiv - Neuroscience 2023

  Quote: ... guinea pig anti-p62 (PROGEN, GP62-C); 1:500 for rabbit anti-TBC1D15 (Sigma-Aldrich ...
• LRRK2 regulates the activation of the unfolded protein response and antigen presentation in macrophages during inflammation

  Ahmed M. Fahmy, et al., bioRxiv - Immunology 2023

  Quote: ... Anti-DNA (61014) was from PROGEN.
• Engineering a highly durable adeno-associated virus receptor for analytical applications

  Kouhei Yoshida, et al., bioRxiv - Bioengineering 2023

  Quote: The AAV Titration ELISA Kit series (PROGEN Biotechnik GmbH) was used to quantify AAV capsid titers depending on the AAV serotype ...
• TAX1BP1 recruits ATG9 vesicles through SCAMP3 binding

  Yutaro Hama, et al., bioRxiv - Molecular Biology 2023

  Quote: ... and guinea pig polyclonal antibodies against p62 (GP62-C; PROGEN) as primary antibodies ...
• Non-ablative disease-modifying effects of magnetic resonance-guided focused ultrasound in neuromelanin-producing parkinsonian rodents

  Joan Compte, et al., bioRxiv - Neuroscience 2023

  Quote: ... guinea pig anti-p62 (1:1000, Progen, Cat#GP62).
• Two birds with one stone: human SIRPα nanobodies for functional modulation and in vivo imaging of myeloid cells

  Teresa R. Wagner, et al., bioRxiv - Immunology 2023

  Quote: ... followed by adding the M13-HRP-labeled detection antibody (Progen, 1:2000 Dilution) for 1 h ...
• Characterization of CAR T Cells Manufactured using Genetically Engineered Artificial Antigen Presenting Cells

  Ali Sayadmanesh, et al., bioRxiv - Immunology 2023

  Quote: ... human PBMCs (peripheral blood mononuclear cells) were isolated from healthy donors using Ficoll-Paque density gradient centrifugation (Progen, 1114544). In each well of a 24-well plate ...
• UBAP2L drives scaffold assembly of nuclear pore complexes at the intact nuclear envelope

  Yongrong Liao, et al., bioRxiv - Cell Biology 2023

  Quote: ... guinea pig polyclonal anti-p62 (Progen, GP62-C), rabbit monoclonal anti-DNAJB6 (Abcam ...
• Peptide ligands for the affinity purification of adeno-associated viruses from HEK 293 cell lysates

  Wenning Chu, et al., bioRxiv - Bioengineering 2023

  Quote: ... The AAV ELISA kits were purchased from Progen (Wayne, PA, USA) while the HEK 293 ELISA kits were purchased from Cygnus (Southport ...
• Peptide ligands for the affinity purification of adeno-associated viruses from HEK 293 cell lysates

  Wenning Chu, et al., bioRxiv - Bioengineering 2023

  Quote: ... and elution fractions collected as described in Section 2.5 and Section 2.6 was measured using a AAV Titration ELISA kit (PROGEN, Wayne, PA) following the manufacturer’s protocol ...
• A mechanism of melanogenesis mediated by E-cadherin downregulation and its involvement in solar lentigines

  Daigo Inoue, et al., bioRxiv - Cell Biology 2023

  Quote: ... anti-Desmoglein-1 (651110,Progen), anti-Desmocollin-1 antibody (HPA012891 ...
• Medium depth influences O₂ availability and metabolism in cultured RPE cells

  Daniel T. Hass, et al., bioRxiv - Cell Biology 2023

  Quote: ... clone AP125 (Progen 610102), 1:10 dilution in 1% BSA in PBS ...
• Corynebacterium diphtheriae causes keratinocyte-intrinsic ribotoxic stress and NLRP1 inflammasome activation in a model of cutaneous diphtheria

  Kim S. ROBINSON, et al., bioRxiv - Immunology 2023

  Quote: ... or plakoglobin (Progen #61005S) using standard DAB staining method.
• Mitochondrial translocation of TFEB regulates complex I and inflammation

  Chiara Calabrese, et al., bioRxiv - Cell Biology 2023

  Quote: ... cells were incubated overnight at 4°C with the primary antibody against mtDNA (61014, Progen), prepared in 3% BSA in PBS ...
• Catalytic antibodies in arrhythmogenic cardiomyopathy patients cleave desmoglein 2 and N-cadherin and impair cardiomyocyte cohesion

  Sunil Yeruva, et al., bioRxiv - Cell Biology 2023

  Quote: ... or polyclonal rabbit anti-DSG2 (Progen, #610121), and monoclonal mouse anti-N-CAD (BD Transduction ...
• Multiparametric domain insertional profiling of Adeno-Associated Virus VP1

  Mareike D. Hoffmann, et al., bioRxiv - Biochemistry 2023

  Quote: ... A primary antibody detecting either all three VP proteins (anti-AAV VP1/VP2/VP3 mouse monoclonal, B1, supernatant, Progen) or only VP1 (anti-AAV VP1 mouse monoclonal ...
• Multiparametric domain insertional profiling of Adeno-Associated Virus VP1

  Mareike D. Hoffmann, et al., bioRxiv - Biochemistry 2023

  Quote: ... or only VP1 (anti-AAV VP1 mouse monoclonal, A, lyophilized, purified, Progen) was diluted 1:250 in 5 % skim milk solution in TBS-T and incubated overnight at 4 °C ...
• Loss of genome maintenance accelerates podocyte damage and aging

  Fabian Braun, et al., bioRxiv - Cell Biology 2023

  Quote: ... Primary antibodies (anti-γH2A.X #2577s – Cell Signalling Technology, anti-nephrin #GP-N2 – Progen, anti-synaptopodin #65294 – Progen ...
• Loss of genome maintenance accelerates podocyte damage and aging

  Fabian Braun, et al., bioRxiv - Cell Biology 2023

  Quote: ... Primary antibodies (anti-γH2A.X #2577s – Cell Signalling Technology, anti-nephrin #GP-N2 – Progen, anti-synaptopodin #65294 – Progen, anti-Dach1 #HPA012672 – Sigma Aldrich (43) ...
• Crucial neuroprotective roles of the metabolite BH4 in dopaminergic neurons

  Shane J. F. Cronin, et al., bioRxiv - Neuroscience 2023

  Quote: ... anti-VMAT2 (Progen, 16085), anti-4-HNE (JaICA ...
• HER2 overexpression initiates breast tumorigenesis non-cell-autonomously by inducing oxidative stress in the tissue microenvironment

  Sevim B. Gurler, et al., bioRxiv - Cancer Biology 2023

  Quote: ... and Anti-Cytokeratin-18 (61028, Progen); with secondary antibodies Donkey Anti-Rabbit AlexaFluor 488 (#A21206 ...
• Parkin is not required to sustain OXPHOS function in adult mammalian tissues

  Roberta Filograna, et al., bioRxiv - Cell Biology 2023

  Quote: ... and anti-DNA (1:200, Progen). Sections were then incubated with secondary antibodies conjugated with Alexa 488 and Alexa 594 at a dilution of 1:500 for 2 hours at RT ...
• Antigen-level resolution of commensal-specific B cell responses enabled by phage-display screening and B cell tetramers

  Sheenam Verma, et al., bioRxiv - Immunology 2023

  Quote: ... 2.5 × 1011 colony forming units (cfu) of the M13K07ΔgIII helper phage “Hyperphage”(Progen) for 30 min at 37 °C ...
• The non-structural proteins NS3 and NS5A of Hepatitis C Virus (HCV) are degraded by two host proteolysis systems

  Sara Mohamed, Jörg Soppa, bioRxiv - Molecular Biology 2023

  Quote: ... and anti-LAMP2 (Guinea pig-polyclonal, Progen Biotechnik, Heidelberg, DE) were used as primary antibodies.
• Evolving Membrane-Associated Accessory Protein Variants for Improved Adeno-associated Virus Production

  Adam J. Schieferecke, et al., bioRxiv - Bioengineering 2023

  Quote: Fully assembled capsids of AAV2 were quantified using sandwich ELISA (PROGEN, AAV Titration ELISA 2.0R) according to manufacturer’s protocol ...
• MAFB drives differentiation by permitting WT1 binding to podocyte 1 specific promoters

  Filippo M Massa, et al., bioRxiv - Developmental Biology 2023

  Quote: ... anti-SYNPO (PROGEN Biotechnik, ref 65194).
• A mitochondrial quality control mechanism reverses the phagosome maturation arrest caused by Mycobacterium tuberculosis

  Surbhi Verma, et al., bioRxiv - Microbiology 2023

  Quote: ... Human MDMs (hMDMs) were isolated from human PBMCs and separated from healthy human donors’ blood using PolymorphPrep (PROGEN,1895) layering and centrifugation ...
• Illuminating the complete ß-cell mass of the human pancreas - signifying a new view on the islets of Langerhans

  Joakim Lehrstrand, et al., bioRxiv - Cell Biology 2023

  Quote: ... Primary antibodies used consisted of guinea pig anti-insulin/pro-insulin (INS; Cat. No. 16049; Progen Biotechnik, Germany; diluted 1:3000) and rabbit anti-glucagon/pro-glucagon (GCG ...
• Heart generation via blastocyst complementation in Mesp1/2-deficient mice

  Yuto Abe, et al., bioRxiv - Cell Biology 2023

  Quote: ... and Vimentin (GP53, Progen, 1:100, and ab45939, Abcam, 1:100) followed by secondary antibodies conjugated with Alexa 488 or 546 and DAPI (D1306 ...
• ALS-related p97 R155H mutation disrupts lysophagy in iPSC-derived motor neurons

  Jacob A. Klickstein, et al., bioRxiv - Neuroscience 2023

  Quote: ... and p62 (GP62-C Progen Biotechnik). Alex Fluor-conjugated secondary antibodies were from Molecular Probes ...