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Citations for GE Life Sciences :
1 - 50 of 3945 citations for tert Butyl 10 oxo 4 9 diazaspiro 4.5 decane 4 carboxylate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... and stirred overnight (O/N) at 4°C with 4.5 g CM Sephadex C-25 beads (GE Healthcare) preequilibrated for 3 h with 100 mL of buffer A ...
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bioRxiv - Synthetic Biology 2024Quote: The OVA protein was first attached to an oligonucleotide using a succinimidyl 4-(N-maleimidomethyl) cyclohexane-1-carboxylate (SMCC) linker and purified using a NAP column (GE Healthcare Life Sciences). Unconjugated oligonucleotides were removed by 30K Amicon filtration (Sigma) ...
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bioRxiv - Biochemistry 2022Quote: ... hydrophobic interaction (Hitrap Butyl HP, GE Healthcare) and gel filtration (Superdex 200 Increase 10/300 ...
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bioRxiv - Biochemistry 2020Quote: ... Recombinant proteins in 10 mM acetate buffer (pH 4.5, GE Healthcare) was then injected over the chip surface at a flow rate of 30 μl/min to couple the amino groups of the recombinant proteins to the carboxymethyl dextran matrix ...
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bioRxiv - Biophysics 2021Quote: ... Nesprin-4 and Nesprin-4-WD/AA were purified via size exclusion chromatography using Superdex 200 10/300 GL (GE Healthcare) column equilibrated in lysis buffer ...
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bioRxiv - Immunology 2023Quote: ... covered in 4+4 Whatman papers (GE Healthcare, UK) soaked in transfer buffer (192 mM glycine ...
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bioRxiv - Physiology 2021Quote: 200 μl of pooled plasma from gender and genotype matched mice (n = 4-9) was loaded onto a Superose 6 column (GE Healthcare) calibrated with elution buffer (0.15 M NaCl ...
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bioRxiv - Biochemistry 2023Quote: ... followed by hydrophobic interaction chromatography (Butyl Sepharose HP, GE Healthcare), and size exclusion chromatography (Superdex 200 16/600 ...
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bioRxiv - Neuroscience 2020Quote: ... Scans were performed using a 4-10 MHz endocavitary transducer (GE Healthcare, Milwaukee, WI). Patients were in lithotomy position with empty bladder ...
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bioRxiv - Biochemistry 2023Quote: ... and subjected to hydrophobic interaction chromatography (Butyl Sepharose HP, GE Healthcare) following a second size exclusion chromatography run in 50 mM Tris-HCl pH 7.9 ...
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bioRxiv - Biochemistry 2023Quote: ... The fractions eluted from anionic exchange column were incubated with ammonium sulfate to a final concentration of 1M and loaded on a Butyl Sepharose High Performance HiLoad16/10 (GE Healthcare) equilibrated with buffer C (25 mM Tris-HCl pH 8.0 ...
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bioRxiv - Molecular Biology 2022Quote: ... for 10 min at 4 °C and injected into Superdex 200 Increase 10/300 GL size-exclusion columns (GE Healthcare). Fractions containing both hZnT7 and Fab were collected and concentrated with a 100-kDa cutoff Amicon filter to around 10 mg ml-1 for cryo-EM measurements.
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bioRxiv - Immunology 2020Quote: ... and incubated overnight at 4°C with Protein G Sepharose 4 Fast Flow (GE Healthcare) overnight ...
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bioRxiv - Biophysics 2023Quote: ... The solution was bound to a HiTrap Butyl HP column (GE Healthcare), washed with 30 cv of high salt buffer (1.5 M NH4SO4 ...
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bioRxiv - Plant Biology 2020Quote: Experiments were conducted at 4°C using a Superdex 75 10/300 GL column (GE Healthcare) equilibrated in running buffer (20 mM HEPES pH 7.5 ...
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A resource for extracellular vesicles from activated CD4 T cells that relay pro-inflammatory signalsbioRxiv - Immunology 2024Quote: ... loaded to Sepharose 4 Fast Flow affinity resin column (10 mm x 300 mm; GE Healthcare) mounted to ÄKTA pure system (GE Healthcare ...
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bioRxiv - Biophysics 2024Quote: ... for 45 min at 4 °C and loaded on an empty PD-10 column (GE Healthcare). The column was washed with 15 column volumes of wash buffer containing 20 mM HEPES ...
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bioRxiv - Cell Biology 2021Quote: ... 10 µg of mESC lysate were separated on 4–12% or 10% SDS-PAGE gels (pre-cast NuPage® GE healthcare or Mini-Protean TGX Stain free precast gels ...
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bioRxiv - Biophysics 2024Quote: ... The sample was centrifuged (10 min, 20000g, 4 °C) and applied to Superdex 200 10/300 gel-filtration column (GE Healthcare) equilibrated with 20 mM Tris-HCl ...
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bioRxiv - Cell Biology 2020Quote: ... 2 µL of a 10 mg/mL mixture of hydrophilic and hydrophobic carboxylate coated paramagnetic beads (SeraMag Speed Beads, #44152105050250 and #24152105050250, GE Healthcare) were added to each sample ...
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bioRxiv - Biochemistry 2023Quote: ... Glutathione-sepharose 4 beads (GE Healthcare) were added to remove the cleaved tags and non-cleaved protein for another 2 h at 4°C in a column under gravity flow ...
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bioRxiv - Neuroscience 2024Quote: ... 2 µg carboxylate modified magnetic beads (GE Healthcare Sera-Mag™) at 1:1 (hydrophilic/hydrophobic ...
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bioRxiv - Microbiology 2024Quote: ... and Carboxylate-Modified Magnetic Particles (35) (GE Life Sciences; GE65152105050250, GE45152105050250). Bead conditioning was done with 3 washes with water at a concentration of 1 µg/µl ...
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bioRxiv - Systems Biology 2024Quote: ... two types of carboxylate-modified SeraMag Speed beads (GE Life Sciences) were combined 1:1 (v/v) ...
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bioRxiv - Cell Biology 2024Quote: ... two types of carboxylate-modified SeraMag Speed beads (GE Life Sciences) were combined in a ratio of 1:1 (v/v) ...
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bioRxiv - Biochemistry 2021Quote: ... pH 7.5 by gel filtration over Sephadex G25 (GE Healthcare, 4 x HiPrep Desalting 26/10 columns).
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bioRxiv - Biochemistry 2021Quote: ... the samples were re-adsorbed onto a Glutathione Sepharose 4 Fast Flow 16/10 column (GE Healthcare) and the flow-through was purified by size exclusion chromatography using a Superdex 200 10/300 GL column (GE Healthcare) ...
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bioRxiv - Biochemistry 2022Quote: ... The resulting supernatant was incubated for 4-5 h at 4 °C on glutathione sepharose 4B (GE Healthcare) (3 mL per 2 L expression) ...
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Targeting properdin - Structure and function of a novel family of tick-derived complement inhibitorsbioRxiv - Immunology 2021Quote: ... The sample was loaded onto a 1-mL HiTrap Butyl HP column (GE Healthcare) and washed with 5 CV of 1.7 M (NH4)2SO4 ...
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bioRxiv - Biochemistry 2020Quote: ... and GEHE65152105050250 Sera-Mag SpeedBead Carboxylate-Modified Magnetic Particles (Hydrophobic, GE Healthcare)).
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bioRxiv - Microbiology 2023Quote: ... 8 µl of SpeedBeads™ magnetic carboxylate modified particles (50% GE Healthcare 45152105050250 and 50% Thermo Scientific 65152105050250 after two times washing with milli-Q water ...
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bioRxiv - Biochemistry 2024Quote: Speedbead magnetic carboxylate-modified particles (GE45152105050250 and GE65152105050250, GE Healthcare, Chicago, IL) were combined in a 1:1 (v/v ...
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bioRxiv - Biochemistry 2021Quote: ... The supernatant was applied and circulated on a Glutathione Sepharose 4 Fast Flow 16/10 column (GE Healthcare) for 16 h ...
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bioRxiv - Microbiology 2021Quote: ... with 4.5 mM L-glutamine (GE Life Sciences) supplemented with 10% FBS (Hyclone ...
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bioRxiv - Biophysics 2022Quote: ... overnight at 4°C then purified over a Sephadex 200 Increase 10/300 (GE Healthcare Life Sciences, Pittsburgh, PA) size exclusion column in Buffer A with 0.02% DDM.
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bioRxiv - Plant Biology 2023Quote: ... Dried eluates were dissolved in 4 M urea with 0.1% ampholytes (pH 3–10; GE Healthcare, Chicago, IL, USA) and fractionated by off-gel focusing ...
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bioRxiv - Plant Biology 2024Quote: Analytical SEC experiments were carried out at 4 °C using a Superdex 75 10/300 GL column (GE Healthcare) equilibrated in SEC running buffer ...
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bioRxiv - Microbiology 2022Quote: ... incubated at 4 °C for 1 hour and further purified by Superose 6 increase 10/300 column (GE Healthcare). The peak tube was concentrated to 0.4-0.5 mg/mL in 20 mM Tris pH8.0 ...
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bioRxiv - Microbiology 2022Quote: ... incubated at 4 °C for 2 hour and further purified by Superose 6 increase 10/300 column (GE Healthcare). The peak tube was concentrated to 0.4-0.5 mg/mL in 20 mM Tris pH8.0 ...
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bioRxiv - Bioengineering 2024Quote: ... 10% glycerol (v/v) at 4°C and re-loaded onto a Ni-NTA column (HisTrapTM HP, GE Healthcare). An imidazole gradient was applied (from 20mM to 500mM ...
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bioRxiv - Microbiology 2024Quote: ... The complex was resolved by gel filtration at 4 °C with a Superdex 200 10/300 column (GE Healthcare) equilibrated with 75 mM KCl ...
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bioRxiv - Biochemistry 2020Quote: ... hydrophobic interaction chromatography using a Hi-Trap Butyl-Sepharose column (GE Healthcare, Cat. No. 28411001) with a flow rate of 1 ml/min and a gradient from 0.9 M (NH4)2SO4 in PBS to PBS over 5 CV was performed ...
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bioRxiv - Biochemistry 2021Quote: ... After centrifugation at 27.000 xg for 30 min at 4 °C the supernatant was incubated with 4 mL Ni2+-Sepharose resin (GE Healthcare) for 1 hour at 4 °C under gentle agitation ...
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bioRxiv - Biochemistry 2021Quote: ... and STD samples were mixed and the rehydration solution (7 M urea, 2 M thiourea, 4% CHAPS, 0.5% IPG Buffer 4-7 [GE Healthcare]) was added before overnight IPG-strips passive rehydration (pH gradient 4-7 ...
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bioRxiv - Cell Biology 2021Quote: ... Immunoprecipitation assays were performed using nProtein A Sepharose 4 Fast Flow or nProtein G Sepharose 4 Fast Flow (GE Healthcare) according to the manufacturer’s instructions ...
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bioRxiv - Systems Biology 2021Quote: ... 300 μg of magnetic beads (1 micron, SpeedBead Magnetic Carboxylate; GE Healthcare UK) was suspended in each sample and protein aggregation was induced by adjusting the sample to 70% acetonitrile ...
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bioRxiv - Immunology 2022Quote: ... and purified using SpeedBead™ carboxylate magnetic bead solution (GE Healthcare, Marlborough, MA). The amplicons were quantified using KAPA Library Quantitation kit (KAPA ...
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bioRxiv - Biochemistry 2021Quote: ... SpeedBeads™ magnetic carboxylate modified particles 45152105050250 and 65152105050250 from Cytiva (GE Healthcare); Protein LoBind® tubes from Eppendorf ...
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bioRxiv - Microbiology 2020Quote: ... DNA bound to Sera-Mag™ SpeedBead magnetic carboxylate-modified particles (GE Healthcare), washed 3 times with ethanol and eluted with 10 mM Tris-HCl pH 8 ...
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bioRxiv - Microbiology 2023Quote: ... 2 µl of carboxylate-modified paramagnetic beads (Sera-Mag Speed Beads, GE Healthcare; Chicago ...