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Citations for GE Life Sciences :
101 - 150 of 281 citations for ssc mir 15a RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: Fixed samples were imaged using a 1.35na 100X objective on an Olympus DeltaVision RT microscope (Applied Precision) with 2×2 binning used for weaker samples and 0.2μm sectioning for z-stacks ...
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bioRxiv - Cell Biology 2022Quote: ... Yeast images were acquired using a Deltavision RT microscope system running softWoRx 3.5.1 software (Applied Precision, LLC), using an Olympus 100× ...
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bioRxiv - Molecular Biology 2023Quote: ... Yeast images were acquired using a Deltavision RT microscope system running softWoRx 3.5.1 software (Applied Precision, LLC), using an Olympus 100× ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Polymerase chain reactions (PCRs) were prepared using PuReTaq Ready-To- Go PCR Beads (GE Healthcare, Chicago, Illinois, USA). Each 25-μl reaction volume contained 19 μl distilled water (dH2O) ...
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bioRxiv - Microbiology 2019Quote: ... PCR products were purified for sequencing by Illustra GFX PCR DNA and Gel Band Purification kit (GE Healthcare). Primers and purified DNA were sent to GATC biotech (Lightrun method ...
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bioRxiv - Immunology 2020Quote: ... the PCR product was purified with the GFX PCR DNA and Gel Band Purification Kit (GE Healthcare, UK) and eluted in dH2O ...
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bioRxiv - Pathology 2020Quote: ... wt:wt) was determined by size exclusion chromatography in AKTA Primer Plus fast protein liquid chromatography system (GE Healthcare) with Superose™ 6 10/30 GL Column (GE Healthcare ...
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bioRxiv - Developmental Biology 2020Quote: ... Flag primary antibody (monoclonal M2 mouse IgG1) and enhanced chemiluminescence assay (Amersham ECL Primer, GE Healthcare Life Science) were used for detection.
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bioRxiv - Microbiology 2021Quote: ... The mAbs were purified from the harvested supernatants using a set of purification steps including MabSelect sure (rProtein A) resin (GE life sciences, now Cytivia), a hydrophobic interaction chromatography (GE lifesciences ...
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bioRxiv - Cell Biology 2020Quote: ... Live-cell imaging were performed using a DeltaVision RT wide-field fluorescence imaging system (Applied Precision, Issaquah, WA) equipped with a quantifiable laser module ...
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bioRxiv - Developmental Biology 2021Quote: ... Secondary antibodies were incubated for 1 hour at RT and signals were detected with ECL Select (GE Healthcare) and Odyssey Fc (Li-Cor) ...
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bioRxiv - Cell Biology 2024Quote: ... and anti-rabbit HRP antibody (50 μL/well of a 1:1000 dilution, GE Healthcare, 1h at RT), with washing steps as above ...
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bioRxiv - Microbiology 2019Quote: ... PCR products were visualized on agarose gel then purified using PCR DNA and Gel Band Purification kit (GE Healthcare, UK).
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bioRxiv - Biochemistry 2021Quote: ... and those containing GsI-IIC RT were pooled and loaded onto a 5-ml Heparin HP column (GE Healthcare) pre-equilibrated in Heparin Buffer A (20 mM Tris-HCl pH 8.5 ...
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bioRxiv - Molecular Biology 2023Quote: ... Z-stack images covering the entire thickness of the samples were acquired on a Delta Vision RT (Applied Precision) restoration microscope using a 100X/1.40 Plan Apo objective ...
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bioRxiv - Microbiology 2022Quote: ... The PCR products were cleaned with an Illustra GFX PCR DNA and gel band purification kit (GE Healthcare, Chicago, IL, USA) and sequenced by using Sanger technology in an ABI 3730XL sequencer (STAB-VIDA ...
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bioRxiv - Microbiology 2022Quote: ... DNA from PCRs and agarose gels was purified using the Illustra GFX PCR DNA and Gel Band Purification Kit (GE Healthcare). Polymerases ...
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bioRxiv - Animal Behavior and Cognition 2020Quote: ... The PCR product was gel purified by Illustra GFX PCR DNA and Gel Band Purification Kits (GE Healthcare, Chicago, Illinois, USA), cloned into pJET 1.2/blunt vector (Thermo Fisher Scientific ...
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bioRxiv - Physiology 2022Quote: ... The membrane was incubated for 1 hour at RT in 2% enhanced chemiluminiscence (ECL) prime blocking agent (GE Healthcare, RPN418). Membranes were incubated overnight at 4⁰C in blocking buffer (4% bovine serum albumin (BSA ...
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bioRxiv - Neuroscience 2020Quote: ... washed three times with 0.1 M cacodylate and then incubated for 3-h at RT in streptavidin HRP conjugate (1:200; GE Healthcare). Sections were visualized after 1-min and 30-sec DAB (Sigma-Aldrich)/glucose oxidase reaction ...
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bioRxiv - Molecular Biology 2020Quote: ... They were then incubated for an hour at RT with 1:5,000 anti-rat horseradish peroxidase conjugated antibody (GE Healthcare). Protein bands were detected on X-ray film using SuperSignal West Pico Chemiluminescent Substrate (Thermo Scientific) ...
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bioRxiv - Microbiology 2021Quote: ... IgG secondary antibody for 1 h at RT and developed in Amersham ECL Western Blotting Detection Kit according to the manufacturer’s instructions (GE Healthcare).
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bioRxiv - Neuroscience 2024Quote: ... followed by 1 h incubation with the respective secondary antibody at RT (HRP-conjugated anti-rabbit, 1/4.000, #NA9340, GE Healthcare; HRP-conjugated anti-mouse ...
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bioRxiv - Neuroscience 2024Quote: ... followed by 1 h incubation with the respective secondary antibody at RT (HRP-conjugated anti-rabbit, 1/4.000, #NA9340, GE Healthcare; HRP-conjugated anti-mouse, 1/3.000, #NA9310, GE Healthcare). An immune signal was detected using Clarity Western ECL substrate (#1705061 ...
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bioRxiv - Bioengineering 2020Quote: ... GFX PCR purification kit was purchased from GE Healthcare.
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bioRxiv - Cancer Biology 2021Quote: ... was performed according to a published protocol 63 except that P32-labeled TS primers were used and the signals were quantified on a Phosphor scanner (FLA7000, GE Healthcare). To determine relative telomerase levels between samples ...
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bioRxiv - Microbiology 2019Quote: ... sidI was amplified using primer pairs SidIBamHI-F/SidINotI-F and cloned as a BamHI/NotI fragment into pGEX-6P-1 (GE Healthcare) and pT7HMT (55) ...
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bioRxiv - Cell Biology 2020Quote: ... was amplified by PCR using the primers (5’-GGTTCCGCGTGGATCCATGTCTCATGCAGCCGAGCCA-3’ and 5’-GGAATTCCGGGGATCCTCAGGACTCCTCTTCAATGCTGA-3’) and cloned into BamHI site of pGEX-4T-1 expression vector (GE Healthcare) using In-Fusion HD Cloning System (Clontech) ...
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bioRxiv - Cell Biology 2019Quote: ... was used for cDNA synthesis using the Ready-to-Go You-Prime It First-Strand beads and random primers (GE Healthcare). RT-PCR reactions were conducted using the GoTaq qPCR Master Mix (A6001 ...
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bioRxiv - Developmental Biology 2022Quote: ... in presence of 32P end-labeled telomeric primers that has been purified using a micro-spin G-25 column (GE healthcare). PCR reactions were resolved by 9% polyacrylamide gel electrophoresis at room temperature ...
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bioRxiv - Biochemistry 2020Quote: ... The amounts of 6-FAM fluorescence in unextended and extended primer bands were quantitated using a Typhoon 9400 scanner and ImageQuant software (GE Healthcare). Single-nucleotide incorporation data were fit to the single exponential equation using GraphPad Prism software ...
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bioRxiv - Microbiology 2022Quote: ... Membranes were probed with primers 32P-labelled with [alpha-P32]Deoxycytidine 5’-triphosphate (dCTP) using Megaprime DNA labelling system (GE Healthcare) (for primers ...
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bioRxiv - Molecular Biology 2020Quote: ... The gels were visualized by the 6-FAM label on the RNA primer using the Typhoon 9500 FLA Imager (GE Healthcare).
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bioRxiv - Molecular Biology 2020Quote: ... and RNA primer 5’-rCrArGrUrGrCrUrArUrGrUr GrArGrArUrUrArArGrUrUrArU-3’ were prepared by solid phase synthesis on an ÄKTA oligopilot plus 10 (GE Healthcare). RNAs were cleaved from the solid support by treating with 4 mL of a 1:1 mixture of 28% wt NH3/H2O solution and 33% wt CH3NH2/EtOH solution at 55°C for 30 minutes ...
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bioRxiv - Biochemistry 2024Quote: ... HRP activity was then detected using the Amersham ECL Primer Western Blotting Detection Reagent (Cytiva) and a LAS4000 chemiluminescence imager (GE Healthcare).
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bioRxiv - Molecular Biology 2019Quote: ... washed 3x with TBS-T and incubated for 1 hours at RT with the secondary antibody (horseradish peroxidase-conjugated sheep anti-mouse or donkey anti-rabbit IgG, GE Healthcare). Membranes were analyzed with a Fusion Solo (Vilber Lourmat) ...
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bioRxiv - Genetics 2021Quote: Images were taken using an Olympus UPlanS APO 100X objective lens (numerical aperture 1.40) on an Olympus IX-70 microscope equipped with the DeltaVision RT (Applied Precision, GE Healthcare) imaging system ...
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bioRxiv - Plant Biology 2021Quote: ... linked to horseradish peroxidase was diluted 1:10000 in TBS and used for one hour at RT before detection by chemiluminescence using AmershamTM ECLTM Prime Western Blotting Detection Reagent (GE Healthcare) following manufactures instructions.
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bioRxiv - Cancer Biology 2020Quote: ... Membranes were incubated with HRP-coupled secondary antibodies (santa cruz, cell signaling) for 1 h at RT and chemiluminescence detected with ECL Prime (GE Healthcare) at the LAS-1000 (Fujifilm) ...
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bioRxiv - Cell Biology 2021Quote: ... the coverslips were placed into a POC-R imaging chamber (Zeiss) within an environmental incubator (Solent Scientific) on an Olympus DeltaVision RT microscope (Applied Precision), with a quantifiable laser module including 488 nm and 405 nm lasers ...
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bioRxiv - Cell Biology 2020Quote: ... Micrographs in time frames of 10 min were acquired during 24 h in a Deltavision RT imaging system (Olympus IX70/71, Applied Precision) equipped with a Plan apochromatic 20X/1.42 N.A ...
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bioRxiv - Neuroscience 2021Quote: ... or 90 V (secreted fraction and serum) at room temperature (RT) and electro-transferred to 0.45 µm nitrocellulose membranes (Amersham, GE Healthcare, Germany) for 1h30-2h at 0.5 A at 4°C ...
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bioRxiv - Molecular Biology 2021Quote: ... 0.2% Triton X-100) and incubated 1 hour at RT or 16 hours at 4°C with HRP-coupled streptavidin (RPN1231VS, GE Healthcare) diluted 1:20000 in BSA blocking buffer ...
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bioRxiv - Microbiology 2022Quote: ... were incubated for 30 min at RT then loaded on a Superdex 200 Increase 10/300 GL column connected to an AKTA purification system (GE Healthcare). The column (bed volume 24 ml ...
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bioRxiv - Neuroscience 2022Quote: ... then cooled to room temperature (RT) and sterilized by filtering through a 0.22 μm syringe filter (GE Healthcare Life Sciences, WHA10462200); aliquots were stored at 4°C ...
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bioRxiv - Immunology 2023Quote: ... The reaction was mixed for 2 hours at room temperature (RT) and then 4M NaCl was added to the derivatized fVi for purification by PD-10 column (GE Healthcare) against water ...
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bioRxiv - Plant Biology 2020Quote: ... MKK7 ORF was PCR amplified from Arabidopsis genomic DNA using primers MKK7-F and MKK7-R1 (Table 2) and cloned into pGEX-5X-1 (GE Healthcare, USA) into BamHI-EcoRI restriction sites ...
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bioRxiv - Molecular Biology 2019Quote: ... random hexamer primers were used following the manufacturer’s instructions for the GenomiPhi V2 DNA Amplification Kit (catalogue # 45-001-221, GE Healthcare Life Sciences).
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bioRxiv - Evolutionary Biology 2022Quote: ... The PCR products were treated with ExoSAP-IT (GE Healthcare) to remove the PCR primers.
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bioRxiv - Zoology 2022Quote: ... PCR products were purified using ExoSAP-IT (GE Healthcare, Buckinghamshire). Purified DNA fragments were used for cycle sequencing reaction with a BigDye Terminator v 1.1 Cycle Sequencing Kit (Applied Biosystems ...