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Citations for GE Life Sciences :
1 - 50 of 4158 citations for Zika Virus VLP E prM M Proteins since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2019Quote: ... The VLP recombinant proteins were further purified by size exclusion chromotogrophy (Superose 6, 10/300 GL;GE Healthcare). The level of endotoxin in all the purified VLPs was measured with an ELISA-based endotoxin detection assay (Hyglos ...
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bioRxiv - Cell Biology 2020Quote: M-PER mammalian protein extraction reagent (GE Healthcare) was used to extract protein from mOSE cells and run on NuPAGE 4-12% Bis-Tris gradient gels (Life Technologies).
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bioRxiv - Immunology 2021Quote: ... Purification of E-301 and E-301-LOF was performed directly from transfection harvests using a HiTrap Protein A affinity column (GE Healthcare) and eluted with 1M arginine pH 3.9 ...
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bioRxiv - Microbiology 2021Quote: ... Fixed VLPs were filtered onto 0.02 μm filter membranes in triplicate (Anodisc, GE Healthcare, USA) and were stained with SYBR-Gold (2.5X concentration) ...
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bioRxiv - Microbiology 2023Quote: ... VLP genomic DNA underwent amplification using the Illustra GenomiPhi V2 DNA Amplification Kit (formerly GE Healthcare Life Sciences but now Cytiva ...
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bioRxiv - Immunology 2021Quote: ... The supernatant was then loaded onto the protein A column and the target protein was eluted with 0.1 M acetic acid on ÄKTA pure (GE Healthcare). The collected protein was added to 1 mM Edetate disodium (EDTA) ...
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bioRxiv - Immunology 2020Quote: ... and VLPs were isolated by Ni-NTA chromatography using a prepacked HisTrap™ HP column (GE Healthcare). SpyCatcher VLPs were eluted with 2.0 M imidazole ...
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bioRxiv - Biochemistry 2020Quote: ... 0.3 M NaCl using a Fast Protein Liquid Chromatography (FPLC) system (ÄKTA Purifier, GE Healthcare Life Sciences). Prior to loading ...
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bioRxiv - Bioengineering 2022Quote: ... His-tagged Ri-boswitch-VLPs were purified using a Ni beads gravity column loaded with high-performance Ni beads (GE Healthcare). Briefly ...
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bioRxiv - Immunology 2020Quote: ... Conjugated VLPs or NPs were then separated from free HA trimers by SEC on a Superose 6 10/300 (GE Healthcare) column equilibrated with PBS (20 mM sodium phosphate pH 7.5 ...
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bioRxiv - Biophysics 2023Quote: ... Protein was purified using a 20 mL NiNTA column (Cytiva) attached to an Akta M FPLC (GE Healthcare) and with a 300 mM imidazole elution buffer ...
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bioRxiv - Cell Biology 2019Quote: ... and 0.5 mM PMSF. Mouse anti-Spt6 antibody (25C3; E. Kremmer/A. Schepers) was coupled to Protein A-Sepharose CL-4B (GE Healthcare) beads and stored at 4°C until actual pulldown ...
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bioRxiv - Genomics 2024Quote: ... WSC3-e cells (1.6 µg protein µL-1) was loaded onto a Superdex 200 10/300 column (GE Healthcare, Chicago, USA), which was equilibrated with a buffer containing 50 mM Tris-HCl (pH 7.84) ...
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bioRxiv - Plant Biology 2020Quote: For NMR structure determination the proteins were buffer exchanged using PD-10 columns (Sephadex G-25 M, GE Healthcare) or via gel filtration chromatography in the final purification step to 20mM MES 6.5 ...
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bioRxiv - Biophysics 2020Quote: ... Protein fractions (eluted at ~ 1 M NaCl) were then purified using a HiLoad 16/60 Superdex 75 (GE Healthcare) column (SEC ...
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bioRxiv - Biochemistry 2021Quote: ... the M-Lipxtal protein was further purified by SEC using a HiLoad Superdex 75 26/600 column (GE Healthcare) equilibrated with SEC Buffer ...
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bioRxiv - Microbiology 2024Quote: ... the hexa-histidine tagged H-Rrm4-M protein was purified using Nickel-based affinity chromatography (HisTrap HP, GE Healthcare) on Akta primeplus FPLC system (GE Healthcare) ...
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bioRxiv - Cell Biology 2020Quote: ... e Image-Quant LAS 4000 (GE Healthcare). Densitometry analysis of the gels was performed using the Image J software (version 1.37) ...
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bioRxiv - Biochemistry 2021Quote: ... anti-EGFR antibody (#MI-12-1, MBL), anti-GFP antibody (#M-048-3, MBL) and Protein A-Sepharose (GE Healthcare) were used for immunoprecipitation ...
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bioRxiv - Plant Biology 2021Quote: ... 2 M thiourea (GE Healthcare), 1% DTT ...
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bioRxiv - Biochemistry 2021Quote: A mixture of 0.5 μM GST or GST tagged cargo receptors and different ATG proteins was incubated with 10 μL Glutathione Sepharose beads (GE Healthcare) in a reaction buffer containing 20 mM HEPES at pH 8.0 ...
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bioRxiv - Microbiology 2020Quote: ... and the soluble His6-M protein was purified on an Nickel sepharose column (HiTrap™ 5 ml IMAC HP; GE Healthcare). The bound protein was washed extensively with loading buffer plus 25 mM imidazole and eluted with a 25 to 250 mM imidazole gradient ...
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bioRxiv - Biophysics 2021Quote: ... was purified by 1 M NaCl containing Tris buffer using AKTATM Pure protein purification system with Hi-Trap Q-FF anion exchange column (GE healthcare). 0.3% H2O2 was added to the purified proteins ...
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bioRxiv - Biochemistry 2023Quote: ... and the soluble His6-M protein was purified on a Nickel sepharose column (HiTrap™ 5 ml IMAC HP; GE Healthcare). The bound protein was washed extensively with loading buffer plus 25 mM imidazole and eluted with a 25 to 250 mM imidazole gradient ...
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bioRxiv - Biophysics 2020Quote: ... which contains DnaK protein was purified first with ion exchange chromatography using 0 to 1 M NaCl in a HiTrap column (HiTrap, GE Healthcare, Germany). Primary fraction of DnaK was eluted in 150 mM NaCl which was further purified using size exclusion chromatography using a superdex 200 column (GE Healthcare ...
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bioRxiv - Microbiology 2021Quote: ... cells were lysed using an M-110S Microfluidizer (Microfluidics) and proteins were purified using glutathione chromatography according to the manufacturer’s instructions (GE Healthcare Life Sciences). To confirm the size and purity of purified recombinant protein ...
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bioRxiv - Microbiology 2020Quote: ... The supernatant was filtered (0.45 μm) and the target protein purified via immobilized metalion affinity chromatography (IMAC) using a 5 mL HisTrap™ HP column (GE Healthcare, UK). The lysis buffer (50 mM Tris/HCl ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Buffer was added to the supernatants to a final concentration of 0.01 M HEPES/0.2 M NaCl (pH 7.4) and passed through a PD-10 desalting column (GE Healthcare) equilibrated with 25 ml of 0.01 M HEPES/0.5mM EDTA (pH 7.2 ...
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bioRxiv - Immunology 2023Quote: ... The peak of properly refolded HLA-G2 was collected and buffer exchanged into HBS-EP (0.01 M HEPES pH 7.4, 0.15 M NaCl, 3 mM EDTA and 0.005% [v/v] Surfactant P20, GE Healthcare) for surface plasmon resonance (SPR ...
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bioRxiv - Neuroscience 2021Quote: ... A Tracerlab FX M module (GE Healthcare) was used for automated methylation ...
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bioRxiv - Cancer Biology 2022Quote: ... M-016083-00-0005 (GE Healthcare Dharmacon). siRNA non-targeting pool #2 (GE Healthcare Dharmacon ...
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bioRxiv - Microbiology 2020Quote: ... Binding analyses were performed in HBS-P buffer (0.01 M HEPES, pH 7.4, 0.15 M NaCl, 0.005% surfactant P20; GE Healthcare Life Sciences) at 37°C with a flow rate of 30 μl/min ...
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bioRxiv - Cell Biology 2023Quote: ... The buffer was exchanged to PBS (0.05 M phosphate buffer, 0.150 M NaCl) using PD-10 desalting columns (GE Healthcare).
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bioRxiv - Cell Biology 2023Quote: ... The buffer was exchanged to PBS (0.05 M phosphate buffer, 0.150 M NaCl) using PD-10 desalting columns (GE Healthcare). Purity of the proteins were evaluated using SDS-PAGE electrophoresis stained with Coomassie blue.
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bioRxiv - Pathology 2021Quote: ... 250 μg of total protein was loaded on a 13 cm Immobiline dry-strip (pH 4-7, M/s. GE Healthcare Life Sciences, Amersham, UK), on a rehydration tray ...
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bioRxiv - Neuroscience 2021Quote: ... the E domain was subcloned into pGEX-6P-1 (GE healthcare). The GST-gephyrin E domain fusion protein was purified from E ...
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bioRxiv - Biochemistry 2020Quote: ... Virus was purified by heparin affinity chromatography on an Akta FPLC (HiTrap, GE Healthcare), ultra-centrifuged overnight and resuspended in sterile Ringer’s solution ...
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bioRxiv - Biophysics 2021Quote: ... to 100% buffer B (6 M urea, 20 mM Tris pH 9.0, 1 M NaCl) of an AKTA FPLC system (GE Healthcare). Fractions containing full-length H1 were pooled and purified on a semi-preparative C18 HPLC column on a gradient of 0—70% buffer B and freeze-dried until use ...
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bioRxiv - Biochemistry 2021Quote: ... and STD samples were mixed and the rehydration solution (7 M urea, 2 M thiourea, 4% CHAPS, 0.5% IPG Buffer 4-7 [GE Healthcare]) was added before overnight IPG-strips passive rehydration (pH gradient 4-7 ...
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bioRxiv - Biochemistry 2021Quote: ... a hydrophobic interaction chromatography using a linear decreasing (NH4)2SO4 gradient (from 1.5 M to 0 M) (HiTrap HIC phenyl column, GE Healthcare) was applied to the mixture ...
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bioRxiv - Cell Biology 2022Quote: ... 6 µL of the reaction were diluted to 100 µL in 2x SSC (3 M NaCl, 0.3 M sodium citrate) and slot-blotted on a Hybond XL nylon membrane (GE Healthcare). For detection of ChIP samples ...
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bioRxiv - Cell Biology 2024Quote: ... The buffer was exchanged to PBS (0.05 M phosphate buffer and 0.150 M NaCl) using PD-10 desalting columns or Midi-Trap G-25 (GE Healthcare). The purity of the proteins was evaluated using SDS–PAGE electrophoresis stained with Coomassie blue.
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bioRxiv - Biochemistry 2020Quote: ... Purified 6-His-PI3Kγ catalytic subunit17,18 was used as the soluble analyte and was injected at different concentrations over the immobilized GRK2 in SPR buffer (0.01 M HEPES pH 7.4, 0.15 M NaCl, 3 mM EDTA, 0.005% surfactant P-20, GE Healthcare Life Sciences, Biacore Inc.). Binding experiments were performed at 25°C in SPR buffer with a flow rate of 20 μl/min ...
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bioRxiv - Biochemistry 2021Quote: ... and eluted with an elution buffer (20 mM NaK, 0.5 M NaCl, and 0.5 M imidazole) in an ÄKTA-Pure instrument (GE Healthcare, Piscataway, NJ). The buffer of the eluted proteins was replaced with a mesotrypsin buffer (100 mM tris(hydroxymethyl)aminomethane (Tris) ...
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bioRxiv - Microbiology 2023Quote: ... using an ÄKTA pure 25 M system (GE Healthcare) and eluted against the same buffer including 500 mM imidazole over 10 column volumes ...
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bioRxiv - Cell Biology 2019Quote: ... HUVECs were cultured in HyClone M-199 media (GE Healthcare) with 1% antibiotics-antimicrobial (Gibco) ...
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bioRxiv - Molecular Biology 2019Quote: ... Chromatography was performed on an AKTApure M system (GE Healthcare) at a flow rate of 0.05 ml/min at 4°C ...
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bioRxiv - Molecular Biology 2019Quote: ... Chromatography was performed on an AKTApure M system (GE Healthcare) at a flow rate of 0.03 ml/min at 4°C ...
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bioRxiv - Microbiology 2022Quote: ... Virus stocks were cleared of residual EdC using a PD-10 desalting column (GE Healthcare, Mississauga, ON) utilizing the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2023Quote: ... The virus-inactivated RPC eluate was loaded on to 0.10 L Macrocap SP Sepharose (GE Healthcare, USA) column to remove RPC buffers and rhEPO aggregates followed by reconstitution into pre-formulation buffer ...