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Citations for GE Life Sciences :
151 - 200 of 661 citations for Zika Virus Lysate DakArD 41662 strain since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... 1 mg lysate was incubated with glutathione agarose resin (GE Healthcare, Chicago, USA) for 2 hours at 4°C under gentle rotation ...
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bioRxiv - Cell Biology 2021Quote: Lysate was loaded on to a 5 ml HisTrap HP cassette (GE Healthcare) equilibrated in lysis buffer ...
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bioRxiv - Biochemistry 2022Quote: ... Clarified lysates were loaded onto a 5 mL HiTrap FF column (GE Healthcare), pre-loaded with Ni2+ and equilibrated with Buffer B (50 mM Tris-HCl ...
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bioRxiv - Microbiology 2022Quote: ... The lysate was loaded into a HisTrap HP 5 mL column (GE Healthcare) previously equilibrated with binding buffer (50 mM Tris ...
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bioRxiv - Cell Biology 2022Quote: ... Total lysate was loaded in a HisTrap HP Ni2+ Sepharose column (GE Healthcare). Column was then extensively washed using lysis buffer ...
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bioRxiv - Biochemistry 2020Quote: ... and lysates were purified by affinity chromatography via AKTA FPLC (GE Life Sciences).
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bioRxiv - Molecular Biology 2020Quote: ... and lysates were cycled over a 5 ml His-Trap column (GE Healthcare) at 4°C overnight ...
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bioRxiv - Cancer Biology 2021Quote: ... The lysates were then centrifuged in G-25 micro spin columns (GE Healthcare). Protein was then quantified from the eluent using the Bradford protein assay ...
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bioRxiv - Biochemistry 2021Quote: ... Cleared lysates were then incubated with Glutathione Sepharose 4 Fast Flow (GE Healthcare) to immobilize GST-fusion proteins.
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bioRxiv - Microbiology 2021Quote: ... the lysate was applied to a 5 ml HisTrap HP column (GE healthcare) and washed with 4 column volumes of 25 mM Tris-HCl pH 7.5 ...
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bioRxiv - Biochemistry 2020Quote: ... Lysates were passed over a 1 ml His-Trap HF column (GE Healthcare). The bound protein was washed with 15 ml high salt wash buffer (20 mM potassium HEPES ...
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bioRxiv - Cell Biology 2020Quote: ... The clarified lysate was separated on a cation exchange column (SP GE healthcare), and bound protein was eluted in cation elution buffer (500 mM NaCl ...
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bioRxiv - Microbiology 2019Quote: ... Cleared lysates were loaded onto a nickel affinity column (HisTrap FF, GE Healthcare) mounted on an Akta chromatography system ...
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bioRxiv - Immunology 2019Quote: ... Clarified lysates were incubated with Glutathione Sepharose 4B resin (GE Healthcare Life Sciences) at a ratio of 1:40 ...
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bioRxiv - Developmental Biology 2020Quote: ... Cell lysates were precleared with GST immobilized on glutathione–Sepharose 4B (GE Healthcare) and then incubated with the indicated GST fusion proteins (2–5 μg ...
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bioRxiv - Microbiology 2019Quote: ... cell lysates were incubated with a DNase/ RNase mixture (1:100, GE Healthcare) for 40 min at 37°C and cell debris was subsequently removed by centrifugation (8,000 g ...
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bioRxiv - Biochemistry 2019Quote: ... and the soluble lysate was purified over a glutathione affinity column (GE Healthcare). Bound complex was eluted via overnight on-column TEV cleavage to remove the GST-and His-tags ...
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bioRxiv - Biochemistry 2021Quote: ... Cleared lysate was applied on a 5 mL MBP-trap column (GE Healthcare) and extensively washed with lysis buffer ...
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bioRxiv - Immunology 2020Quote: ... lysate was affinity purified using Cobalt-charged His-Trap Chelating column (GE Healthcare) through imidazole gradient elution ...
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bioRxiv - Molecular Biology 2020Quote: ... the lysate was loaded on a 5 mL HisTrap HP column (GE Healthcare) washed with Ni wash buffer (25 mM HEPES pH 7.5 ...
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bioRxiv - Microbiology 2022Quote: ... The lysates were loaded into a HisTrap HP 5 mL column (GE Healthcare) previously equilibrated with binding buffer (50 mM Tris ...
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bioRxiv - Cancer Biology 2022Quote: ... Lysates were spun down and supernatant was loaded over Ni-NTA (GE Healthcare). For conversion to Fc-fusion ...
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bioRxiv - Microbiology 2022Quote: ... Clarified lysate was loaded onto a 5ml FFcrude prepacked nickel column (GE Healthcare) pre-equilibrated with buffer A ...
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bioRxiv - Microbiology 2022Quote: ... Cell lysate was loaded to a pre-equilibrated HisTrap Crude FF (GE Healthcare) column ...
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bioRxiv - Biochemistry 2022Quote: ... the soluble lysate was loaded onto a HiTrap XL SP column (GE Healthcare) for cation exchange chromatography and eluted with a 0-0.5 M NaCl gradient ...
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bioRxiv - Neuroscience 2023Quote: ... the lysate was purified using glutathione S-transferase beads (Ge healthcare, #17-5132). The proteins were eluted and concentrated with Amicon Centrifugal Filter Units 10K (Millipore ...
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bioRxiv - Molecular Biology 2022Quote: ... The lysate was fractionated on a HisTrapTM HP 1 mL column (GE Healthcare) using a linear gradient from sonication buffer to elution buffer (sonication buffer with 250 mM imidazole) ...
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bioRxiv - Biochemistry 2022Quote: ... The lysates were loaded onto a 1 ml HisTrap HP column (GE healthcare) coupled to an ÄKTA Pure FPLC system (GE healthcare) ...
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bioRxiv - Molecular Biology 2023Quote: ... Lysates were pre-cleared with protein A-Agarose beads (GE Healthcare, Chicago, USA) for 1 h in a wheel shaker at 4°C ...
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bioRxiv - Microbiology 2023Quote: Clarified lysate was applied to a 5 ml HisTrap HP Column (GE Healthcare) equilibrated with buffer A (50 mM Tris-HCl at the appropriate pH ...
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bioRxiv - Molecular Biology 2023Quote: ... Filtered lysates were loaded onto a 5 mL HisTrap HP column (GE Healthcare), which was washed with four CV of low salt buffer ...
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bioRxiv - Cancer Biology 2023Quote: ... cell lysates were directly incubated with GlutathioneSepharose 4B (52-2303-00; GE Healthcare) overnight at 4°C ...
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bioRxiv - Biochemistry 2023Quote: ... The lysate was loaded onto a 5 ml HisTrap FF column (GE Healthcare), equilibrated in His-A buffer (50 mM HEPES-NaOH pH 8.5 ...
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bioRxiv - Cell Biology 2022Quote: ... Cell lysate supernatant was loaded on a Glutathione Sepharose 4B column (GE Healthcare). The column was washed with TBS (20 mM Tris ...
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bioRxiv - Cell Biology 2023Quote: ... The protein was purified from the lysate using Glutathione-sepharose 4B (GE Healthcare). His-tagged protein expression was induced by shifting to 15°C and adding 0.1 mM IPTG ...
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bioRxiv - Microbiology 2023Quote: ... The lysates were loaded into a HisTrap HP 5 mL column (GE Healthcare) previously equilibrated with binding buffer (50 mM Tris ...
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bioRxiv - Molecular Biology 2023Quote: ... The lysate was applied to a 1 ml HisTrap HP column (GE Healthcare) and eluted with 300 mM imidazole in the same buffer without lysozyme ...
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bioRxiv - Molecular Biology 2024Quote: ... The lysates were loaded onto a 1 ml HisTrap HP column (GE Healthcare) coupled to an ÄKTA Pure FPLC system (GE Healthcare) ...
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bioRxiv - Microbiology 2023Quote: ... Cleared lysate was incubated with washed IgG Sepharose 6 Fast-Flow (GE Healthcare) for 3 hours at 4 °C ...
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bioRxiv - Immunology 2024Quote: ... Lysate pre-cleaning steps were performed using Protein G Sepharose beads (GE Healthcare). To measure the protein content of the lysate a Qubit 4 fluorometer was used using the Qubit protein assay kit (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2022Quote: ... the PCR amplified eleven positive virus genomes were amplified by RCA (rolling circle amplification) using the RCA-based TempliPhi DNA amplification kit (GE Healthcare) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... The lysate was then incubated with Glutathione Sepharose 4B beads (GE Healthcare Life Sciences) previously washed 3 times with cold PBS ...
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bioRxiv - Cell Biology 2020Quote: The clarified lysates were loaded onto 5mL HisTrap FF Ni-NTA columns (GE Healthcare) equilibrated in 4°C column buffer containing 50mM Tris-HCl pH 8.0 ...
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bioRxiv - Molecular Biology 2021Quote: ... Cleared lysate was loaded onto a 5 mL HisTrap sepharose HP column (GE Healthcare) equilibrated with buffer A ...
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bioRxiv - Biophysics 2022Quote: ... The cleared lysate was applied into glutathione-Sepharose 4B resin (GE Healthcare, Piscataway, NJ) overnight in 4°C on a nutator ...
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bioRxiv - Cancer Biology 2019Quote: ... Cleared lysates were incubated with protein G Mag Sepharose™ Xtra beads (GE Healthcare) and 1µg of antibody anti-ISG15 per sample for 4h ...
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bioRxiv - Biochemistry 2019Quote: ... The cleared lysate was loaded onto a pre-equilibrated HisTrap Crude FF (GE Healthcare) column ...
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bioRxiv - Biochemistry 2019Quote: ... The cleared lysate was loaded onto a 5 ml HisTrap HP column (GE Healthcare), and was washed with stepwise increases of imidazole in Buffer A ...
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bioRxiv - Molecular Biology 2019Quote: ... chromatin lysates were incubated for 4 hours with protein-G sepharose beads (GE Healthcare). Beads were washed 3× with low-salt buffer (150 mM NaCl ...
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bioRxiv - Cell Biology 2019Quote: ... the lysate was loaded on a HisTrap FF Crude column (GE healthcare Life Sciences). The column was then washed with a speed of 1 ml/min with guanidium buffer ...